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    Method to screen phage display libraries with different ligands
    63.
    发明申请
    Method to screen phage display libraries with different ligands 审中-公开
    用不同配体筛选噬菌体展示文库的方法

    公开(公告)号：US20070111282A1

    公开(公告)日：2007-05-17

    申请号：US11469556

    申请日：2006-09-01

    申请人： Ian Tomlinson ,  Gregory Winter

    发明人： Ian Tomlinson ,  Gregory Winter

    IPC分类号： C07K16/18 ,  C12P21/06 ,  C12N5/06

    CPC分类号： C07K1/047 ,  C07K14/705 ,  C07K14/70503 ,  C07K14/7051 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/21 ,  C07K2317/622 ,  C12N15/1037 ,  C40B30/04 ,  C40B40/02 ,  G01N33/6845 ,  G01N33/6854

    摘要： The invention provides a method for selecting, from a repertoire of polypeptides, a population of functional polypeptides which bind a target ligand in a first binding site and a generic ligand in a second binding site, which generic ligand is capable of binding functional members of the repertoire regardless of target ligand specificity, comprising the steps of: a) contacting the repertoire with the generic ligand and selecting functional polypeptides bound thereto; and b) contacting the selected functional polypeptides with the target ligand and selecting a population of polypeptides which bind to the target ligand. The invention accordingly provides a method by which a polypeptide repertoire is preselected, according to functionality as determined by the ability to bind the generic ligand, and the subset of polypeptides obtained as a result of such preselection is then employed for further selection according to the ability to bind the target ligand.

    摘要翻译： 本发明提供了从多肽的所有组成部分中选择结合第一结合位点中的靶配体和第二结合位点中的通用配体的功能性多肽群体的方法，该通用配体能够结合 包括以下步骤：a）使所述谱系与通用配体接触并选择与其结合的功能性多肽; 和b）使所选择的功能性多肽与靶配体接触并选择结合靶配体的多肽群体。 因此，本发明提供了根据通过结合通用配体的能力确定的官能度来预先选择多肽集合的方法，然后根据该预选的能力进一步选择作为这种预选结果的多肽获得的子集 以结合靶配体。

    Method to screen phage display libraries with different ligands
    69.
    发明授权
    Method to screen phage display libraries with different ligands 有权
    用不同配体筛选噬菌体展示文库的方法

    公开(公告)号：US06846634B1

    公开(公告)日：2005-01-25

    申请号：US09511939

    申请日：2000-02-24

    申请人： Ian Tomlinson ,  Greg Winter

    发明人： Ian Tomlinson ,  Greg Winter

    IPC分类号： G01N33/50 ,  C07K1/04 ,  C07K14/705 ,  C07K14/725 ,  C07K16/00 ,  C12N15/09 ,  C12N15/10 ,  C12P21/08 ,  C40B30/04 ,  C40B40/02 ,  G01N33/15 ,  G01N33/68 ,  G01N33/53 ,  C07H21/04 ,  C12Q1/70

    CPC分类号： C07K1/047 ,  C07K14/705 ,  C07K14/70503 ,  C07K14/7051 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/21 ,  C07K2317/622 ,  C12N15/1037 ,  C40B30/04 ,  C40B40/02 ,  G01N33/6845 ,  G01N33/6854

    摘要： The invention provides a method for selecting, from a repertoire of polypeptides, a population of functional polypeptides which bind a target ligand in a first binding site and a generic ligand in a second binding site, which generic ligand is capable of binding functional members of the repertoire regardless of target ligand specificity, comprising the steps of: a) contacting the repertoire with the generic ligand and selecting functional polypeptides bound thereto; and b) contacting the selected functional polypeptides with the target ligand and selecting a population of polypeptides which bind to the target ligand. The invention accordingly provides a method by which a polypeptide repertoire is preselected, according to functionality as determined by the ability to bind the generic ligand, and the subset of polypeptides obtained as a result of such preselection is then employed for further selection according to the ability to bind the target ligand.

    摘要翻译： 本发明提供了从多肽的所有组成部分中选择结合第一结合位点中的靶配体和第二结合位点中的通用配体的功能性多肽群体的方法，该通用配体能够结合 包括以下步骤：a）使所述谱系与通用配体接触并选择与其结合的功能性多肽; 和b）使所选择的功能性多肽与靶配体接触并选择结合靶配体的多肽群体。 因此，本发明提供了根据通过结合通用配体的能力所确定的官能度来预先选择多肽集合的方法，然后根据能力进一步选择作为这种预选的结果的多肽获得的子集 以结合靶配体。

    Direct screening method
    70.
    发明申请
    Direct screening method 审中-公开
    直接筛选方法

    公开(公告)号：US20030039958A1

    公开(公告)日：2003-02-27

    申请号：US10161145

    申请日：2002-05-31

    申请人： Domantis Limited

    发明人： Lucy Jessica Holt ,  Rudolph Maria Theodora De Wildt ,  Ian Michael Tomlinson

    IPC分类号： C12Q001/70 ,  C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  C07K016/00

    CPC分类号： C40B30/04 ,  G01N33/6842 ,  G01N33/6845 ,  G01N33/6854

    摘要： The invention concerns a method for screening a repertoire of polypeptides to identify one otr more members thereof which interact with one or more target molecules, comprising: a) immobilising the target molecule(s) on a support; b) arranging a plurality of nucleic acis molecules encoding the repertoire of polypeptides in an array; c) juxtaposing the target molecule(s) and the arrayed nucleic acid molecules; d) expressing the arrayed nucleic acid molecules to produce the polypeptides such that said polypeptides come into contact with the target molecule(s) on the support and a subset of the polypeptides interacts with the target molecules; and e) detecting the interaction of the polypeptides with target molecules on the support. The invention also provides a high density antibody array consisting of thousands of different polypeptide features, spatially arranged on a solid support for screening against different target ligands.

    摘要翻译： 本发明涉及一种用于筛选多肽的所有组成成分以识别与一个或多个靶分子相互作用的其它成员的方法，其包括：a）将靶分子固定在载体上; b）将编码多肽的所有组成成分的多个核酸分子排列成阵列; c）并置目标分子和排列的核酸分子; d）表达排列的核酸分子以产生多肽，使得所述多肽与载体上的靶分子接触，并且多肽的一个子集与靶分子相互作用; 和e）检测多肽与载体上靶分子的相互作用。 本发明还提供了由数千种不同多肽特征组成的高密度抗体阵列，空间上排列在用于筛选不同靶配体的固体支持物上。