公开(公告)号：US08911945B2

公开(公告)日：2014-12-16

申请号：US14318352

申请日：2014-06-27

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia Van Eijk ,  Anker Preben Sørensen ,  Marco Gerardus Maria Van Schriek

IPC: C12Q1/68 ,  C12P19/34 ,  C40B20/00 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6874 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2565/301 ,  C12Q2535/138 ,  C12Q2535/122 ,  C12Q2527/107

Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.

Abstract translation: 本发明涉及一种用于一种或多种样品中一种或多种遗传标记物的高通量发现，检测和基因分型的方法，其包括以下步骤：DNA的限制性内切核酸酶消化，衔接子连接，任选的预扩增，选择性扩增，合并 的扩增产物，对文库以足够的冗余进行测序，聚类，然后鉴定文库内和/或文库之间的遗传标记和确定遗传标记的（共）优势基因型。

公开(公告)号：US20140274744A1

公开(公告)日：2014-09-18

申请号：US14253806

申请日：2014-04-15

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia Van EIJK ,  Henricus Johannes Adam Van Der Poel

IPC: C12Q1/68

CPC classification number: G06F19/22 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q2600/156 ,  C40B30/04 ,  C40B30/10 ,  C12Q2539/107 ,  C12Q2531/113 ,  C12Q2525/191

Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.

公开(公告)号：US20140259223A1

公开(公告)日：2014-09-11

申请号：US14352608

申请日：2012-10-19

Applicant: Keygene N.V.

Inventor： Hendrik Jan Bouwmeester ,  Maurice Gerard Leon Henquet ,  Maarten Anthonie Jongsma

IPC: C12N9/04 ,  C12N15/82 ,  C12P7/02 ,  C12P17/04

CPC classification number: C12P7/02 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/16 ,  C12N15/8241 ,  C12N15/8286 ,  C12P17/04

Abstract: Described is a nucleic acid sequence isolated from Persicaria hydropiper and encoding a drimenol oxidase protein, expression vectors comprising such nucleic acid sequence, chimeric genes comprising such nucleic acid sequence, host cells or host organisms altered to harbour the drimenol oxidase nucleic acid sequence, and the drimenol oxidase protein itself. Methods for producing cinnamolide and/or drimendiol and/or enhanced levels of cinnamolide and/or drimendiol, in a cell or organism harbouring such nucleic acid sequence are provided. Transgenic organisms comprising the nucleic acid sequence or a chimeric gene of the invention are also provided. The present invention especially relates to transgenic plants with enhanced resistance to insects and enhanced insect antifeedant properties.

Abstract translation: 描述了从Persicaria hydropiper分离的核酸序列，并编码一种石蜡氧化酶蛋白，包含该核酸序列的表达载体，包含该核酸序列的嵌合基因，被改变为含有该二醇氧化酶核酸序列的宿主细胞或宿主生物，以及 石蜡氧化酶蛋白本身。 提供了在含有这种核酸序列的细胞或生物体中生产肉桂酸酯和/或烈im醇和/或增强的肉桂酸酯和/或烈end醇水平的方法。 还提供了包含本发明的核酸序列或嵌合基因的转基因生物。 本发明特别涉及具有增强的对昆虫的抗性和增强的昆虫抗食性质的转基因植物。

公开(公告)号：US12215329B2

公开(公告)日：2025-02-04

申请号：US16485806

申请日：2018-02-15

Applicant: Keygene N.V.

Inventor： Paul Bundock

IPC: C12N15/82 ,  C12N9/22 ,  C12N9/78 ,  C12N15/11

Abstract: The current invention relates to methods of targeted genetic alteration in cells, preferably plant cells, as well as to plant cells and plants thus obtained using at least a fusion protein comprising a site-specific nuclease domain and a deaminase domain, or a construct encoding the same. The method also provides for a composition and a kit comprising a combination of a first fusion protein comprising a cytosine deaminase domain and a second fusion protein comprising an adenine deaminase domain, preferably for use in the method of the invention. The method provides for targeted alteration of a DNA duplex in plant cells with increased efficacy.

公开(公告)号：US20240324531A1

公开(公告)日：2024-10-03

申请号：US18737058

申请日：2024-06-07

Applicant: Keygene N.V.

Inventor： Natalia CARRENO-QUINTERO ,  Tatyana Mitkova RADOEVA ,  René Johannes Maria HOFSTEDE

IPC: A01H1/02 ,  A01G7/00 ,  A01H5/10

CPC classification number: A01H1/027 ,  A01G7/00 ,  A01H5/10

Abstract: The invention pertains to a method for increasing plant pollen viability using a MRN-ATM pathway inhibitor. The increased pollen viability preferably results in increased plant viability. The invention further pertains to an MRN-ATM pathway inhibitor for increasing plant pollen viability. A preferred MRN-ATM pathway inhibitor for use in the invention is 2-Amino-5-[(4-hydroxyphenyl)methylene]-4(5H)-thiazolone. The invention also pertains to a method for developing a mature fertile plant graft comprising contacting an isolated plant part comprising an immature flower bud with a (hazardous or toxic) compound.

公开(公告)号：US20240002904A1

公开(公告)日：2024-01-04

申请号：US18038381

申请日：2021-11-24

Applicant: KEYGENE N.V.

Inventor： Theodorus Frank Maria ROELOFS ,  René Cornelis Josephus HOGERS

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: The current invention pertains to a method for sequencing of a target nucleic acid fragment from a nucleic acid sample, comprising the steps of cleaving the nucleic acid sample with a first and a second RNA guided or DNA guided endonuclease complex, preferably a first and a second gRNA-CAS complex, thereby generating the target nucleic acid fragment and at least one non-target nucleic acid fragment. The generated fragments are subsequently contacted with an exonuclease, wherein the exonuclease digests only the non-target nucleic acid fragments. Subsequently said target nucleic acid fragment is sequenced using nanopore selective sequencing. The invention further pertains to the use of the enriched target nucleic acid fragments for nanopore selective sequencing the target nucleic acid fragment.

公开(公告)号：US20230383308A1

公开(公告)日：2023-11-30

申请号：US18133392

申请日：2023-04-11

Applicant: Keygene N.V.

Inventor： Rik Hubertus Martinus OP DEN CAMP ,  Peter Johannes VAN DIJK

IPC: C12N15/82 ,  A01H1/02

CPC classification number: C12N15/8287 ,  A01H1/022

Abstract: The invention provides a method to produce a mutant gene, wherein said gen comprises a modified promoter and wherein said gene is capable of inducing the parthenogenesis phenotype to a plant. The invention further provides said mutant gene, isolated nucleic acid molecule, construct or vector comprising the same. Also, the invention provides for a method to produce a parthenogenetic plant comprising the mutant gene, and the parthenogenetic plant thus obtained.

公开(公告)号：US20230304030A1

公开(公告)日：2023-09-28

申请号：US17984938

申请日：2022-11-10

Applicant: Keygene N.V.

Inventor： Diana RIGOLA ,  René Johannes Maria HOFSTEDE ,  Magdalena Barbara LASTDRAGER ,  Jamila Maguy CHAÏB

IPC: C12N15/82 ,  C12Q1/6895 ,  A01H5/08

CPC classification number: C12N15/8282 ,  A01H5/08 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: The present invention relates to genetic elements comprising powdery mildew-conferring QTLs derived from a plant of the species Cucumis melo, or powdery mildew-conferring part or variant thereof. The invention also relates to markers for identification of said QTLs, use thereof and methods for producing plants with increased resistance to powdery mildew and the plants thus obtained.

公开(公告)号：US11649494B2

公开(公告)日：2023-05-16

申请号：US16719863

申请日：2019-12-18

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia Van Eijk ,  Adrianus Johannes Van Tunen

IPC: C12Q1/6874 ,  G16B30/00 ,  C12Q1/6858 ,  C12Q1/6869 ,  G16B30/10 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6851

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  C12Q1/6869 ,  G16B30/00 ,  G16B30/10 ,  C12Q2600/13 ,  C12Q1/6858 ,  C12Q2525/155 ,  C12Q2563/179 ,  C12Q1/6869 ,  C12Q2563/155 ,  C12Q2537/143

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

公开(公告)号：US11519003B2

公开(公告)日：2022-12-06

申请号：US17037506

申请日：2020-09-29

Applicant: Keygene N.V.

Inventor： Diana Rigola ,  René Johannes Maria Hofstede ,  Magdalena Barbara Lastdrager ,  Jamila Maguy Chaïb

IPC: C12N15/82 ,  A01H5/08 ,  C12Q1/6895

Abstract: The present invention relates to genetic elements comprising powdery mildew-conferring QTLs derived from a plant of the species Cucumis melo, or powdery mildew-conferring part or variant thereof. The invention also relates to markers for identification of said QTLs, use thereof and methods for producing plants with increased resistance to powdery mildew and the plants thus obtained.