公开(公告)号：US09365867B2

公开(公告)日：2016-06-14

申请号：US13796263

申请日：2013-03-12

Applicant: THE CATHOLIC UNIVERSITY OF AMERICA

Inventor： Venigalla B. Rao

IPC: C12N7/00 ,  C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  C12N15/87 ,  C12N15/88 ,  C07K14/005 ,  A61K47/48 ,  A61K48/00

CPC classification number: C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  A61K47/48776 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2795/10042 ,  C12N2795/10122 ,  C12N2795/10142 ,  C12N2795/10152

Abstract: Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.

Abstract translation: 复杂病毒通过顺序和不可逆组装由简单蛋白质亚基组装。 在噬菌体的基因组包装过程中，一个强大的分子马达组装在一个空头颅的特殊门户顶点，开始包装。 本发明的一个方面涉及高度混杂的噬菌体T4包装机，将DNA转运到成品噬菌体头以及pro头中。 单个电机可以将外源DNA以与头孢子相同的速率强制进入噬菌体头，并且噬菌体头经历反复引发，将多个DNA分子包装在相同的头中。 这表明噬菌体DNA包装机具有不寻常的构象可塑性，将DNA提供到明显被动的衣壳容器中，包括高度稳定的病毒壳，直至其充满。 这些特征允许设计一种新型的纳米框输送车辆。

公开(公告)号：US09187765B2

公开(公告)日：2015-11-17

申请号：US14337545

申请日：2014-07-22

Applicant: The Catholic University of America

Inventor： Venigalla B. Rao

IPC: C12Q1/68 ,  C12N15/87

CPC classification number: C12N15/87 ,  C07K2319/10 ,  C07K2319/40 ,  C07K2319/61 ,  C12N2795/10122 ,  C12N2795/10123 ,  C12N2795/10142 ,  C12N2810/6009 ,  C12N2810/85 ,  C12N2810/859

Abstract: Described is T4 DNA packaging machine comprising: one or more DNA molecules packaged in a head of the T4 DNA packaging machine, one or more Hoc-fused proteins displayed on the head of the T4 DNA packaging machine, and one or more Soc-fused proteins displayed on the head of the T4 DNA packaging machine. Also described are methods of making and using such a T4 DNA packaging machine.

Abstract translation: 描述的是T4 DNA包装机，其包括：包装在T4 DNA包装机的头部中的一个或多个DNA分子，显示在T4 DNA包装机头部上的一种或多种Hoc融合蛋白，以及一种或多种Soc-融合蛋白 显示在T4 DNA包装机的头上。 还描述了制备和使用这种T4 DNA包装机的方法。

公开(公告)号：US09163262B2

公开(公告)日：2015-10-20

申请号：US14096238

申请日：2013-12-04

Applicant: The Catholic University of America

Inventor： Venigalla B. Rao

IPC: C12Q1/68 ,  C12N15/87

CPC classification number: C12N15/87 ,  C07K2319/10 ,  C07K2319/40 ,  C07K2319/61 ,  C12N2795/10122 ,  C12N2795/10123 ,  C12N2795/10142 ,  C12N2810/6009 ,  C12N2810/85 ,  C12N2810/859

Abstract: Described is T4 DNA packaging machine comprising: one or more DNA molecules packaged in a head of the T4 DNA packaging machine, one or more Hoc-fused proteins displayed on the head of the T4 DNA packaging machine, and one or more Soc-fused proteins displayed on the head of the T4 DNA packaging machine. Also described are methods of making and using such a T4 DNA packaging machine.

Abstract translation: 描述的是T4 DNA包装机，其包括：包装在T4 DNA包装机的头部中的一个或多个DNA分子，显示在T4 DNA包装机头部上的一种或多种Hoc融合蛋白，以及一种或多种Soc-融合蛋白 显示在T4 DNA包装机的头上。 还描述了制备和使用这种T4 DNA包装机的方法。

公开(公告)号：US20150190783A1

公开(公告)日：2015-07-09

申请号：US14412892

申请日：2013-08-20

Applicant: UNIVERSITY OF MARYLAND ,  THE CATHOLIC UNIVERSITY OF AMERICA

Inventor： Chanel Tissot ,  Aaron Barkatt ,  Mohamad I. Al-Sheikhly

IPC: B01J20/30 ,  C02F1/28 ,  B01J20/26 ,  C08G69/00

CPC classification number: B01J20/3085 ,  B01J20/264 ,  B01J20/265 ,  B01J20/28023 ,  B01J20/28038 ,  B01J20/2804 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3278 ,  B01J20/3425 ,  B01J20/3475 ,  C02F1/285 ,  C02F1/683 ,  C02F2101/006 ,  C02F2103/08 ,  C02F2303/16 ,  C08G69/00 ,  C22B60/0265 ,  G21F9/12

Abstract: Complexing or chelating agents that offer strong, selective bonding with uranium as well as a broad pH range of effectiveness, specifically including the pH range around 8.2, together with the acrylic double bonds required for radiation-induced grafting on polymers to remove uranium from a solution such as seawater. The novel adsorbing species are phosphorus-containing molecules, in particular organic phosphates, phosphonates and phosphoric acids. Organic phosphorus compounds, for example, organic phosphates, phosphonates, and phosphoric acids, are attached to polymer fibers to form fibers, fiber fabrics or membranes that are effective, or show activity, in uranium adsorption.

Abstract translation: 提供强大的选择性结合铀的复合或螯合剂以及广泛的pH范围的有效性，特别是包括pH范围在8.2左右，以及辐射诱导接枝聚合物从溶液中除去铀所需的丙烯酸双键 如海水。 新颖的吸附物质是含磷分子，特别是有机磷酸盐，膦酸盐和磷酸。 将有机磷化合物，例如有机磷酸盐，膦酸盐和磷酸连接到聚合物纤维上以形成在铀吸附中有效或显示活性的纤维，纤维织物或膜。

公开(公告)号：US20140264140A1

公开(公告)日：2014-09-18

申请号：US14193001

申请日：2014-02-28

Applicant: The Catholic University of America

Inventor： Weiliang GONG ,  Werner LUTZE ,  Ian L. PEGG

IPC: C04B28/02

CPC classification number: C04B38/02 ,  C04B28/006 ,  Y02P40/165 ,  Y02W30/92 ,  Y02W30/94 ,  Y02W30/95 ,  C04B12/04 ,  C04B14/06 ,  C04B14/106 ,  C04B14/28 ,  C04B14/303 ,  C04B18/08 ,  C04B18/141 ,  C04B18/162 ,  C04B20/002 ,  C04B20/0048 ,  C04B20/008 ,  C04B22/04 ,  C04B22/06 ,  C04B38/08 ,  C04B2103/0088 ,  C04B2103/22 ,  C04B2103/58

Abstract: A composite binder comprises: one or more Class F fly ash materials, one or more gelation enhancers, and one or more hardening enhancers, wherein each of the one or more Class F fly ash materials comprises 15 wt. % or less calcium oxide, and wherein the composite binder is a Portland cement-free binder for concrete. Also provided are Geopolymer Composite Cellular Concretes (GCCCs) including the composite binder and methods of making these GCCCs.

Abstract translation: 复合粘合剂包括：一种或多种F级飞灰材料，一种或多种胶凝增强剂和一种或多种硬化增强剂，其中所述一种或多种F级飞灰材料中的每一种包含15wt。 ％以下的氧化钙，并且其中复合粘合剂是用于混凝土的波特兰无水泥粘合剂。 还提供了包括复合粘合剂的地质聚合物复合蜂窝混凝土（GCCC）和制备这些GCCC的方法。