公开(公告)号：US20130196416A1

公开(公告)日：2013-08-01

申请号：US13796263

申请日：2013-03-12

申请人： THE CATHOLIC UNIVERSITY OF AMERICA

发明人： Venigalla B. Rao

IPC分类号： C12N15/88

CPC分类号： C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  A61K47/48776 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2795/10042 ,  C12N2795/10122 ,  C12N2795/10142 ,  C12N2795/10152

摘要： Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.

摘要翻译： 复杂病毒通过顺序和不可逆组装由简单蛋白质亚基组装。 在噬菌体的基因组包装过程中，一个强大的分子马达组装在一个空头颅的特殊门户顶点，开始包装。 本发明的一个方面涉及高度混杂的噬菌体T4包装机，将DNA转运到成品噬菌体头以及pro头中。 单个电机可以将外源DNA以与头孢子相同的速率强制进入噬菌体头，并且噬菌体头经历反复的引发，将多个DNA分子包装在相同的头中。 这表明噬菌体DNA包装机具有不寻常的构象可塑性，将DNA提供到明显被动的衣壳容器中，包括高度稳定的病毒壳，直至其充满。 这些特征允许设计一种新型的纳米框输送车辆。

公开(公告)号：US09765122B2

公开(公告)日：2017-09-19

申请号：US14652645

申请日：2014-01-20

申请人： University of Washington through its Center for Commercialization

发明人： Carlos Enrique Catalano ,  Jenny Ren-Jye Chang

IPC分类号： A61K38/16 ,  C12N7/00 ,  C12N7/01 ,  C12N15/00 ,  C07K14/005 ,  A61K38/17

CPC分类号： C07K14/005 ,  A61K38/177 ,  C07K2319/735 ,  C12N7/00 ,  C12N2795/10023 ,  C12N2795/10042

摘要： The present invention provides viral-based nanoparticles for therapeutic and diagnostic use, and methods for making and using the nanoparticles. Specifically, such nanoparticles comprise decoration-competent viral particles shells such as expanded capsids of phages, stabilized with engineered decoration proteins that have been linked to one or more compounds not naturally occurring on a wild type viral capsid.

公开(公告)号：US20160348097A1

公开(公告)日：2016-12-01

申请号：US15167224

申请日：2016-05-27

申请人： Radiant Genomics, Inc.

发明人： Oliver LIU ,  Jeffrey Hoon KIM

IPC分类号： C12N15/10 ,  C12N7/00 ,  C12N15/76

CPC分类号： C12N15/1072 ,  C12N7/00 ,  C12N15/63 ,  C12N15/76 ,  C12N2795/10042 ,  C12N2795/10043

摘要： The disclosure provides compositions and methods for producing natural products in microorganisms that are otherwise unexpressed, poorly expressed or poorly transcribed. In particular aspects, the disclosure provides compositions and methods for activating a silent gene or gene cluster with a bacteriophage and/or Streptomyces Antibiotic Regulatory Protein (SARP) transcription factor.

摘要翻译： 本公开提供了用于在微生物中生产天然产物的组合物和方法，否则其未表达，表达差或转录不良。 在具体方面，本公开提供了用噬菌体和/或抗链霉菌抗生素调节蛋白（SARP）转录因子激活沉默基因或基因簇的组合物和方法。

公开(公告)号：US08802418B2

公开(公告)日：2014-08-12

申请号：US13082466

申请日：2011-04-08

申请人： Venigalla B. Rao

发明人： Venigalla B. Rao

IPC分类号： C12Q1/70 ,  C12N7/00

CPC分类号： C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  A61K47/48776 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2795/10042 ,  C12N2795/10122 ,  C12N2795/10142 ,  C12N2795/10152

摘要： Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.

摘要翻译： 复杂病毒通过顺序和不可逆组装由简单蛋白质亚基组装。 在噬菌体的基因组包装过程中，一个强大的分子马达组装在一个空头颅的特殊门户顶点，开始包装。 本发明的一个方面涉及高度混杂的噬菌体T4包装机，将DNA转运到成品噬菌体头以及pro头中。 单个电机可以将外源DNA以与头孢子相同的速率强制进入噬菌体头，并且噬菌体头经历反复引发，将多个DNA分子包装在相同的头中。 这表明噬菌体DNA包装机具有不寻常的构象可塑性，将DNA提供到明显被动的衣壳容器中，包括高度稳定的病毒壳，直至其充满。 这些特征允许设计一种新型的纳米框输送车辆。

公开(公告)号：US09523101B2

公开(公告)日：2016-12-20

申请号：US14322097

申请日：2014-07-02

申请人： The Catholic University of America

发明人： Venigalla B. Rao

IPC分类号： C12N15/86 ,  C12N15/87 ,  C07K14/005 ,  C12N7/00 ,  C12N15/88 ,  A61K47/48 ,  A61K31/7088 ,  A61K31/711 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  A61K47/48776 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2795/10042 ,  C12N2795/10122 ,  C12N2795/10142 ,  C12N2795/10152

摘要： Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.

公开(公告)号：US20140335158A1

公开(公告)日：2014-11-13

申请号：US14322097

申请日：2014-07-02

申请人： The Catholic University of America

发明人： Venigalla B. Rao

IPC分类号： C12N15/86 ,  A61K47/48 ,  C12N7/00

CPC分类号： C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  A61K47/48776 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2795/10042 ,  C12N2795/10122 ,  C12N2795/10142 ,  C12N2795/10152

摘要： Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.

摘要翻译： 复杂病毒通过顺序和不可逆组装由简单蛋白质亚基组装。 在噬菌体的基因组包装过程中，一个强大的分子马达组装在一个空头颅的特殊门户顶点，开始包装。 本发明的一个方面涉及高度混杂的噬菌体T4包装机，将DNA转运到成品噬菌体头以及pro头中。 单个电机可以将外源DNA以与头孢子相同的速率强制进入噬菌体头，并且噬菌体头经历反复引发，将多个DNA分子包装在相同的头中。 这表明噬菌体DNA包装机具有不寻常的构象可塑性，将DNA提供到明显被动的衣壳容器中，包括高度稳定的病毒壳，直至其充满。 这些特征允许设计一种新型的纳米框输送车辆。

公开(公告)号：US20180057542A1

公开(公告)日：2018-03-01

申请号：US15692909

申请日：2017-08-31

申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

发明人： CARLOS ENRIQUE CATALANO ,  JENNY REN-JYE CHANG

IPC分类号： C07K14/005 ,  C12N7/00 ,  A61K38/17

CPC分类号： C07K14/005 ,  A61K38/177 ,  C07K2319/735 ,  C12N7/00 ,  C12N2795/10023 ,  C12N2795/10042

摘要： The present invention provides viral-based nanoparticles for therapeutic and diagnostic use, and methods for making and using the nanoparticles. Specifically, such nanoparticles comprise decoration-competent viral particles shells such as expanded capsids of phages, stabilized with engineered decoration proteins that have been linked to one or more compounds not naturally occurring on a wild type viral capsid.

公开(公告)号：US09365867B2

公开(公告)日：2016-06-14

申请号：US13796263

申请日：2013-03-12

申请人： THE CATHOLIC UNIVERSITY OF AMERICA

发明人： Venigalla B. Rao

IPC分类号： C12N7/00 ,  C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  C12N15/87 ,  C12N15/88 ,  C07K14/005 ,  A61K47/48 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  A61K47/48776 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2795/10042 ,  C12N2795/10122 ,  C12N2795/10142 ,  C12N2795/10152

摘要： Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.

摘要翻译： 复杂病毒通过顺序和不可逆组装由简单蛋白质亚基组装。 在噬菌体的基因组包装过程中，一个强大的分子马达组装在一个空头颅的特殊门户顶点，开始包装。 本发明的一个方面涉及高度混杂的噬菌体T4包装机，将DNA转运到成品噬菌体头以及pro头中。 单个电机可以将外源DNA以与头孢子相同的速率强制进入噬菌体头，并且噬菌体头经历反复引发，将多个DNA分子包装在相同的头中。 这表明噬菌体DNA包装机具有不寻常的构象可塑性，将DNA提供到明显被动的衣壳容器中，包括高度稳定的病毒壳，直至其充满。 这些特征允许设计一种新型的纳米框输送车辆。

公开(公告)号：US20150337014A1

公开(公告)日：2015-11-26

申请号：US14652645

申请日：2014-01-20

申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

发明人： Carlos Enrique CATALANO ,  Jenny Ren-Jye CHANG

IPC分类号： C07K14/005

CPC分类号： C07K14/005 ,  A61K38/177 ,  C07K2319/735 ,  C12N7/00 ,  C12N2795/10023 ,  C12N2795/10042

摘要： The present invention provides viral-based nanoparticles for therapeutic and diagnostic use, and methods for making and using the nanoparticles. Specifically, such nanoparticles comprise decoration-competent viral particles shells such as expanded capsids of phages, stabilized with engineered decoration proteins that have been linked to one or more compounds not naturally occurring on a wild type viral capsid.

摘要翻译： 本发明提供用于治疗和诊断用途的基于病毒的纳米颗粒，以及制备和使用纳米颗粒的方法。 具体地，这样的纳米颗粒包括装饰性病毒颗粒壳，例如扩增的噬菌体衣壳，用工程改造的装饰蛋白稳定，其已经连接到一种或多种天然存在于野生型病毒衣壳上的化合物。

公开(公告)号：US20110250263A1

公开(公告)日：2011-10-13

申请号：US13082466

申请日：2011-04-08

申请人： Venigalla B. RAO

发明人： Venigalla B. RAO

IPC分类号： A61K9/127 ,  C12N7/00 ,  A61K31/7088 ,  A61P43/00 ,  C12N5/00 ,  C12N5/04 ,  A61K31/711 ,  C12N1/00 ,  A61K47/42

CPC分类号： C12N15/86 ,  A61K31/7088 ,  A61K31/711 ,  A61K47/48776 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2795/10042 ,  C12N2795/10122 ,  C12N2795/10142 ,  C12N2795/10152

摘要： Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.

摘要翻译： 复杂病毒通过顺序和不可逆组装由简单蛋白质亚基组装。 在噬菌体的基因组包装过程中，一个强大的分子马达组装在一个空头颅的特殊门户顶点，开始包装。 本发明的一个方面涉及高度混杂的噬菌体T4包装机，将DNA转运到成品噬菌体头以及pro头中。 单个电机可以将外源DNA以与头孢子相同的速率强制进入噬菌体头，并且噬菌体头经历反复引发，将多个DNA分子包装在相同的头中。 这表明噬菌体DNA包装机具有不寻常的构象可塑性，将DNA提供到明显被动的衣壳容器中，包括高度稳定的病毒壳，直至其充满。 这些特征允许设计一种新型的纳米框输送车辆。