公开(公告)号：US20100159586A1

公开(公告)日：2010-06-24

申请号：US12440015

申请日：2007-09-07

申请人： Feng Tian ,  Thea Norman ,  Stephanie Chu

发明人： Feng Tian ,  Thea Norman ,  Stephanie Chu

IPC分类号： C12N5/071 ,  C07H21/02

CPC分类号： C12P21/02 ,  C12N9/93 ,  C12N15/11

摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in vertebrate cells. The components include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNA's/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in vertebrate cells are also provided. The present invention provides vertebrate cells with translation components, e.g., pairs of orthogonal aminoacyl-tRNA synthetases (O-RSs) and orthogonal tRNA's (O-tRNA's) and individual components thereof, that are used in vertebrate protein biosynthetic machinery to incorporate an unnatural amino acid in a growing polypeptide chain, in a vertebrate cell.

摘要翻译： 本发明提供用于生产翻译成分的组合物和方法，其扩展脊椎动物细胞中遗传编码氨基酸的数目。 组分包括正交tRNA，正交氨酰-tRNA合成酶，tRNA /合成酶和非天然氨基酸的正交对。 还提供了在脊椎动物细胞中产生具有非天然氨基酸的蛋白质的蛋白质和方法。 本发明提供具有翻译成分的脊椎动物细胞，例如正交氨基-tRNA合成酶（O-RS）和正交tRNA（O-tRNA）及其各自成分的对，其用于脊椎动物蛋白质生物合成机制以掺入非天然氨基 在生长的多肽链中，在脊椎动物细胞中。

公开(公告)号：US20100159585A1

公开(公告)日：2010-06-24

申请号：US12440013

申请日：2007-09-07

申请人： Feng Tian ,  Thea Norman ,  Stephanie Chu

发明人： Feng Tian ,  Thea Norman ,  Stephanie Chu

IPC分类号： C12N5/071 ,  C12N15/74 ,  C12N5/10

CPC分类号： C12N15/85 ,  C12N9/93 ,  C12N15/11 ,  C12P21/02

摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in vertebrate cells. The components include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNA's/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in vertebrate cells are also provided.

摘要翻译： 本发明提供用于生产翻译成分的组合物和方法，其扩展脊椎动物细胞中遗传编码氨基酸的数目。 组分包括正交tRNA，正交氨酰-tRNA合成酶，tRNA /合成酶和非天然氨基酸的正交对。 还提供了在脊椎动物细胞中产生具有非天然氨基酸的蛋白质的蛋白质和方法。

公开(公告)号：US07632823B2

公开(公告)日：2009-12-15

申请号：US11293629

申请日：2005-12-01

申请人： Feng Tian

发明人： Feng Tian

IPC分类号： A61K48/00 ,  C07K14/61 ,  C07H21/02 ,  C12P21/06

CPC分类号： C12N15/11 ,  C12N9/93 ,  C12P21/02

摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.

摘要翻译： 提供了包括正交tRNA，正交氨酰-tRNA合成酶和正交对tRNA /合成酶的蛋白质生物合成机理组分的组合物和方法。 还提供用于鉴定这些正交对的方法以及使用这些正交对产生蛋白质的方法。

公开(公告)号：US20090137736A1

公开(公告)日：2009-05-28

申请号：US12092265

申请日：2006-11-08

申请人： Feng Tian ,  Zhenwei Miao

发明人： Feng Tian ,  Zhenwei Miao

IPC分类号： C07K1/107

CPC分类号： C07K14/61 ,  C07K1/1077

摘要： Disclosed herein are accelerants for the formation of oxime-containing compounds from the reaction of a carbonyl-containing compound and a hydroxylamine-containing compound. The oxime-containing compound, the carbonyl-containing compound and the hydroxylamine-containing compound can each be a non-natural amino acid or a non-natural amino acid polypeptide. Also disclosed is the use of such accelerants to form oxime-containing compounds, the resulting oxime-containing compounds, and reaction mixtures containing such accelerants.

摘要翻译： 本文公开了用于由含羰基化合物和含羟胺化合物的反应形成含肟化合物的促进剂。 含肟化合物，含羰基化合物和含羟胺化合物各自可以是非天然氨基酸或非天然氨基酸多肽。 还公开了使用这种促进剂来形成含肟化合物，所得到的含肟化合物和含有这种促进剂的反应混合物。

公开(公告)号：US20090018029A1

公开(公告)日：2009-01-15

申请号：US12092791

申请日：2006-11-16

申请人： Zhenwei Miao ,  Feng Tian ,  Anna-Maria A. Hays Putnam ,  Ying Buechler

发明人： Zhenwei Miao ,  Feng Tian ,  Anna-Maria A. Hays Putnam ,  Ying Buechler

IPC分类号： C40B40/10 ,  G01N33/53 ,  G01N33/68 ,  C40B30/00 ,  C07K1/14 ,  C40B30/04 ,  C12Q1/68

CPC分类号： G01N33/6812

摘要： Disclosed herein are methods of detecting non-natural amino acids and polypeptides that include at least one non-natural amino acid. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of functionalities, including but not limited to oxime, carbonyl, and/or hydroxylamine groups. Also disclosed herein are non-natural amino acid polypeptides that are further modified post-translationally, and methods for detecting such polypeptides.

摘要翻译： 本文公开了检测包括至少一种非天然氨基酸的非天然氨基酸和多肽的方法。 非天然氨基酸本身或作为多肽的一部分可以包括宽范围的官能团，包括但不限于肟，羰基和/或羟胺基团。 本文还公开了在翻译后进一步修饰的非天然氨基酸多肽，以及用于检测这些多肽的方法。

公开(公告)号：US20080179948A1

公开(公告)日：2008-07-31

申请号：US12024734

申请日：2008-02-01

申请人： Siddharth P. Nagarkatti ,  Yevgeniy Barskiy ,  Feng Tian ,  Ilya Bystryak

发明人： Siddharth P. Nagarkatti ,  Yevgeniy Barskiy ,  Feng Tian ,  Ilya Bystryak

IPC分类号： H02J1/10 ,  H03G3/20 ,  H01J15/04

CPC分类号： H03F3/20 ,  H01J37/32082 ,  H01J37/32183 ,  H03F1/565 ,  H03F3/195 ,  H03F2200/321 ,  H03F2200/387 ,  H03F2200/391 ,  H03F2200/393 ,  H03F2200/451 ,  H03H7/40 ,  Y10T307/305

摘要： A system and method are provided for delivering power to a dynamic load. The system includes a power supply providing DC power having a substantially constant power open loop response, a power amplifier for converting the DC power to RF power, a sensor for measuring voltage, current and phase angle between voltage and current vectors associated with the RF power, an electrically controllable impedance matching system to modify the impedance of the power amplifier to at least a substantially matched impedance of a dynamic load, and a controller for controlling the electrically controllable impedance matching system. The system further includes a sensor calibration measuring module for determining power delivered by the power amplifier, an electronic matching system calibration module for determining power delivered to a dynamic load, and a power dissipation module for calculating power dissipated in the electrically controllable impedance matching system.

摘要翻译： 提供了一种将功率传递给动态负载的系统和方法。 该系统包括提供具有基本上恒定的功率开环响应的DC电力的电源，用于将DC功率转换为RF功率的功率放大器，用于测量与RF功率相关联的电压和电流矢量之间的电压，电流和相位角的传感器 ，电可控阻抗匹配系统，用于将功率放大器的阻抗修改为动态负载的至少基本匹配的阻抗;以及用于控制电可控阻抗匹配系统的控制器。 该系统还包括用于确定由功率放大器输出的功率的传感器校准测量模块，用于确定输送到动态负载的功率的电子匹配系统校准模块，以及用于计算在电气可控阻抗匹配系统中消耗的功率的功率耗散模块。

公开(公告)号：US20080139794A1

公开(公告)日：2008-06-12

申请号：US11875741

申请日：2007-10-19

申请人： Feng Tian ,  Zhenwei Miao

发明人： Feng Tian ,  Zhenwei Miao

IPC分类号： C07K2/00

CPC分类号： C07K14/61 ,  C07K1/1077

摘要： Disclosed herein are accelerants for the formation of oxime-containing compounds from the reaction of a carbonyl-containing compound and a hydroxylamine-containing compound. The oxime-containing compound, the carbonyl-containing compound and the hydroxylamine-containing compound can each be a non-natural amino acid or a non-natural amino acid polypeptide. Also disclosed is the use of such accelerants to form oxime-containing compounds, the resulting oxime-containing compounds, and reaction mixtures containing such accelerants.

摘要翻译： 本文公开了用于由含羰基化合物和含羟胺化合物的反应形成含肟化合物的促进剂。 含肟化合物，含羰基化合物和含羟胺化合物各自可以是非天然氨基酸或非天然氨基酸多肽。 还公开了使用这种促进剂来形成含肟化合物，所得到的含肟化合物和含有这种促进剂的反应混合物。

公开(公告)号：US09133495B2

公开(公告)日：2015-09-15

申请号：US12440015

申请日：2007-09-07

申请人： Feng Tian ,  Thea Norman ,  Stephanie Chu

发明人： Feng Tian ,  Thea Norman ,  Stephanie Chu

IPC分类号： C12N15/85 ,  C12P21/02 ,  C12N9/00 ,  C12N15/11

CPC分类号： C12P21/02 ,  C12N9/93 ,  C12N15/11

摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in vertebrate cells. The components include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNA's/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in vertebrate cells are also provided. The present invention provides vertebrate cells with translation components, e.g., pairs of orthogonal aminoacyl-tRNA synthetases (O-RSs) and orthogonal tRNA's (O-tRNA's) and individual components thereof, that are used in vertebrate protein biosynthetic machinery to incorporate an unnatural amino acid in a growing polypeptide chain, in a vertebrate cell.

摘要翻译： 本发明提供用于生产翻译成分的组合物和方法，其扩展脊椎动物细胞中遗传编码氨基酸的数目。 组分包括正交tRNA，正交氨酰-tRNA合成酶，tRNA /合成酶和非天然氨基酸的正交对。 还提供了在脊椎动物细胞中产生具有非天然氨基酸的蛋白质的蛋白质和方法。 本发明提供具有翻译成分的脊椎动物细胞，例如正交氨基-tRNA合成酶（O-RS）和正交tRNA（O-tRNA）及其各自成分的对，其用于脊椎动物蛋白质生物合成机制以掺入非天然氨基 在生长的多肽链中，在脊椎动物细胞中。

公开(公告)号：US08943259B2

公开(公告)日：2015-01-27

申请号：US12946971

申请日：2010-11-16

申请人： Boris Weissman ,  Aleksandr V. Mirgorodskiy ,  Ganesh Venkitachalam ,  Feng Tian

发明人： Boris Weissman ,  Aleksandr V. Mirgorodskiy ,  Ganesh Venkitachalam ,  Feng Tian

IPC分类号： G06F9/455 ,  G06F12/02 ,  G06F17/30

CPC分类号： G06F9/45558 ,  G06F12/023 ,  G06F17/3048 ,  G06F2009/45583 ,  G06F2212/151 ,  G06F2212/163

摘要： Memory of a database management system (DBMS) that is running in a virtual machine is managed using techniques that integrate DBMS memory management with virtual machine memory management. Because of the integration, the effectiveness of DBMS memory management is preserved even though the physical memory allocated to the virtual machine may change during runtime as a result of varying memory demands of other applications, e.g., instances of other virtual machines, running on the same host computer as the virtual machine.

摘要翻译： 使用将DBMS内存管理与虚拟机内存管理集成的技术来管理在虚拟机中运行的数据库管理系统（DBMS）的内存。 由于集成，即使分配给虚拟机的物理内存可能在运行时间内由于其他应用程序的不同内存需求（例如，运行在其上的其他虚拟机的实例）的结果而改变，DBMS内存管理的有效性也将保持不变 主机作为虚拟机。

公开(公告)号：US20140228256A1

公开(公告)日：2014-08-14

申请号：US14345671

申请日：2012-01-10

申请人： Jianguang Zhang ,  Yang Gao ,  Yanbin Shi ,  Di Chen ,  Feng Tian

发明人： Jianguang Zhang ,  Yang Gao ,  Yanbin Shi ,  Di Chen ,  Feng Tian

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N15/1093 ,  C12Q2521/525 ,  C12Q2525/191 ,  C12Q2535/122

摘要： The disclosure relates a method and a kit for constructing a plasma Deoxyribonucleic acid (DNA) sequencing library. The method provided by the disclosure includes: extracting a plasma DNA; making the plasma DNA ligate to a sequencing linker, and purifying a ligation product; performing Polymerase Chain Reaction (PCR) amplification for the purified ligation product, purifying the PCR amplification product, and obtaining the plasma DNA sequencing library, wherein, the method does not include the step of performing 5′-terminus phosphorylation for the plasma DNA. The kit provided by the disclosure includes: a reagent which ligates a plasma DNA to a sequencing linker, including the sequencing linker, a ligase and a ligation buffer; and reagents and instruments for purifying the ligation product; a reagent which performs PCR amplification for a purified ligation product, and reagents and instruments for purifying the PCR amplification product; wherein, the kit does not include the reagent which performs 5′-terminus phosphorylation for the plasma DNA. The disclosure simplifies the construction flow of a plasma DNA sequencing library, simplifies the experimental procedures, and makes the construction of the plasma sample library have lower cost, higher efficiency and faster speed, and is convenient for large-scale application.

摘要翻译： 本公开涉及用于构建等离子体脱氧核糖核酸（DNA）测序文库的方法和试剂盒。 本公开提供的方法包括：提取血浆DNA; 使血浆DNA与测序接头连接，并纯化连接产物; 对纯化的连接产物进行聚合酶链反应（PCR）扩增，纯化PCR扩增产物，获得血浆DNA测序文库，其中不包括对血浆DNA进行5'-末端磷酸化的步骤。 由本公开提供的试剂盒包括：将血浆DNA连接到测序接头的试剂，包括测序接头，连接酶和连接缓冲液; 和用于纯化连接产物的试剂和仪器; 用于纯化连接产物进行PCR扩增的试剂，以及用于纯化PCR扩增产物的试剂和仪器; 其中，试剂盒不包括对血浆DNA进行5'-末端磷酸化的试剂。 本发明简化了血浆DNA测序文库的构建流程，简化了实验程序，使得等离子体样品库的构建成本更低，效率更高，速度更快，方便大规模应用。