Method for the recombinant production of 1,3-propanediol
    63.
    发明授权
    Method for the recombinant production of 1,3-propanediol 失效
    1,3-丙二醇重组生产方法

    公开(公告)号:US06953684B2

    公开(公告)日:2005-10-11

    申请号:US09308207

    申请日:1997-11-13

    摘要: The present invention provides a microorganism for the production of 1,3-propanediol from a variety of carbon sources in an organism capable of 1,3-propanediol production and comprising a) at least one gene encoding a dehydratase activity; b) at least one gene encoding a glycerol-3-phosphatase; and c) at least one gene encoding protein X. The protein X may be derived from a Klebsiella or Citrobacter gene cluster. The recombinant microorganism may further comprise d) at least one gene encoding a protein having at least 50% similarity to a protein selected from the group consisting of protein 1 (SEQ ID NO:60 or SEQ ID NO:61), of protein 2 (SEQ ID NO:62 or SEQ ID NO:63) and of protein 3 (SEQ ID NO:64 or SEQ ID NO:65) from Klebsiella or Citrobacter.

    摘要翻译: 本发明提供一种用于在能够进行1,3-丙二醇生产的生物体中从多种碳源生产1,3-丙二醇的微生物,其包含:a)至少一种编码脱水酶活性的基因; b)至少一种编码甘油-3-磷酸酶的基因; 和c)至少一个编码蛋白X的基因。蛋白X可以来自克雷伯菌或柠檬酸杆菌基因簇。 重组微生物还可以包含d)至少一种编码与蛋白质2(SEQ ID NO:60或SEQ ID NO:61)蛋白质具有至少50%相似性的蛋白质的基因,蛋白质2(SEQ ID NO:60或SEQ ID NO:61) SEQ ID NO:62或SEQ ID NO:63)和来自克雷伯杆菌或柠檬酸杆菌的蛋白质3(SEQ ID NO:64或SEQ ID NO:65)。

    Cell culture compositions
    64.
    发明授权
    Cell culture compositions 有权
    细胞培养组合物

    公开(公告)号:US08674062B2

    公开(公告)日:2014-03-18

    申请号:US11938954

    申请日:2007-11-13

    IPC分类号: C07K7/08

    摘要: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl6 (SEQ ID NO:1 encodes the full length endoglucanase; SEQ ID NO:4 encodes the mature form), and the corresponding endoglucanase VI amino acid sequence (“EGVI”; SEQ ID NO:3 is the signal sequence; SEQ ID NO:2 is the mature sequence). The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVI, recombinant EGVI proteins and methods for producing the same.

    摘要翻译: 本发明提供了一种命名为egl6(SEQ ID NO:1编码全长内切葡聚糖酶; SEQ ID NO:4编码成熟形式)的新型内切葡聚糖酶核酸序列和相应的内切葡聚糖酶VI氨基酸序列(“EGVI”; SEQ ID NO: ID NO:3是信号序列; SEQ ID NO:2是成熟序列)。 本发明还提供了包含编码EGVI的核酸序列,重组EGVI蛋白的表达载体和宿主细胞及其制备方法。