利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

72 条记录 (耗时 0.038 秒)

    Methods of targeting T-cells to tumors
    61.
    发明授权
    Methods of targeting T-cells to tumors 有权
    将T细胞靶向肿瘤的方法

    公开(公告)号：US09561291B2

    公开(公告)日：2017-02-07

    申请号：US13838667

    申请日：2013-03-15

    申请人： Imre Kovesdi ,  Tibor Bakács ,  Miklós Szabó

    发明人： Imre Kovesdi ,  Tibor Bakács ,  Miklós Szabó

    IPC分类号： A61K47/48

    CPC分类号： A61K47/48746 ,  A61K47/48615 ,  A61K47/4863 ,  A61K47/48638 ,  A61K47/48753 ,  A61K47/6863 ,  A61K47/6867 ,  A61K47/6869 ,  A61K47/6898

    摘要： The invention provides a method of targeting T-cells to tumor cells using a tumor-associated antigen (TAA) specific antibody and a T-cell specific antibody, wherein the two antibodies can bind to each other through a high affinity avidin/biotin or streptavidin/biotin connection. The invention further provides methods to target activated T-cells to different tumor types by using a T-cell specific antibody that is specific to an activated T-cell surface molecule like CTLA-4.

    摘要翻译： 本发明提供了使用肿瘤相关抗原（TAA）特异性抗体和T细胞特异性抗体将T细胞靶向肿瘤细胞的方法，其中两种抗体可以通过高亲和素亲和素/生物素或链霉抗生物素蛋白 /生物素连接。 本发明还提供了通过使用对活化的T细胞表面分子如CTLA-4特异性的T细胞特异性抗体来将活化的T细胞靶向不同肿瘤类型的方法。

    Materials and methods for treating ocular-related disorders
    62.
    发明申请
    Materials and methods for treating ocular-related disorders 审中-公开
    用于治疗眼部相关疾病的材料和方法

    公开(公告)号：US20050220768A1

    公开(公告)日：2005-10-06

    申请号：US11138931

    申请日：2005-05-26

    申请人： Duncan McVey ,  Douglas Brough ,  Imre Kovesdi ,  Lisa Wei

    发明人： Duncan McVey ,  Douglas Brough ,  Imre Kovesdi ,  Lisa Wei

    IPC分类号： A61K20060101 ,  A61K9/00 ,  A61K31/203 ,  A61K31/7088 ,  A61K38/55 ,  A61K45/06 ,  A61K48/00 ,  C12N15/85 ,  C12N15/86 ,  C12N15/861

    CPC分类号： A61K48/005 ,  A61K9/0048 ,  A61K31/203 ,  A61K31/7088 ,  A61K38/57 ,  A61K45/06 ,  A61K48/00 ,  A61K48/0058 ,  A61K48/0075 ,  A61K48/0083 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2799/022 ,  A61K2300/00

    摘要： The invention is directed to a method of delivering a gene product to an animal. The method comprises administering an expression vector comprising a nucleic acid sequence operably linked to a promoter and encoding a gene product, and upregulating transcription of the nucleic acid sequence in the ocular cell. The expression vector can be an adenoviral vector. The invention further provides a method of prophylactically or therapeutically treating an animal for at least one ocular-related disorder. The method comprises contacting an ocular cell with an expression vector comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and/or a neurotrophic agent. In one aspect, the method further comprises upregulating transcription of the nucleic acid sequence. Preferably, if 2×108 adenoviral particles of the inventive method are administered to a mouse, the level of expression of the nucleic acid sequence is not diminished more than ten-fold at 28 days post-administration.

    摘要翻译： 本发明涉及将基因产物递送至动物的方法。 该方法包括施用包含与启动子可操作地连接并编码基因产物的核酸序列的表达载体，以及上调眼细胞中核酸序列的转录。 表达载体可以是腺病毒载体。 本发明还提供了预防性或治疗性地治疗动物至少一种眼部相关疾病的方法。 该方法包括使眼细胞与包含编码血管发生抑制剂和/或神经营养剂的核酸序列的表达载体接触。 在一个方面，该方法还包括上调核酸序列的转录。 优选地，如果向小鼠施用本发明方法的2×10 8个腺病毒颗粒，则在给药后28天，核酸序列的表达水平不会降低10倍以上。

    Method of enhancing bone density
    63.
    发明授权
    Method of enhancing bone density 失效
    提高骨密度的方法

    公开(公告)号：US06939540B1

    公开(公告)日：2005-09-06

    申请号：US09629074

    申请日：2000-07-31

    申请人： Ronald G. Crystal ,  Chisa Hidaka ,  Oheneba Boachie-Adjei ,  Bernard A. Rawlins ,  Imre Kovesdi

    发明人： Ronald G. Crystal ,  Chisa Hidaka ,  Oheneba Boachie-Adjei ,  Bernard A. Rawlins ,  Imre Kovesdi

    IPC分类号： A61K38/19 ,  A61K48/00 ,  A61P19/08 ,  A61P19/10 ,  C12N5/28 ,  A01N63/00 ,  A01N43/04 ,  C12N5/00 ,  C12N15/00

    CPC分类号： A61K38/1875 ,  A61K38/18 ,  A61K38/1841 ,  A61K38/1866 ,  A61K48/00 ,  C12N2799/022 ,  A61K2300/00

    摘要： The present invention is directed to a method for enhancing bone density or formation. In accordance with the method, a nucleic acid encoding an angiogenic protein is administered to a cell in a region of a bone such that the nucleic acid is expressed to produce the angiogenic protein, whereby bone density or formation is enhanced within the region. Optionally, a nucleic acid encoding an osteogenic protein is administered to a cell within the same region such that the nucleic acid is expressed to produce the osteogenic protein. The method can be employed to produce a bone graft having a cell harboring an exogenous nucleic acid encoding an angiogenic protein and, optionally, a cell harboring a nucleic acid encoding an osteogenic protein. To facilitate the inventive method, the invention also pertains to a recombinant viral vector having a nucleic acid encoding an angiogenic protein and a nucleic acid encoding an osteogenic protein.

    摘要翻译： 本发明涉及一种增强骨密度或形成的方法。 根据该方法，将编码血管发生蛋白的核酸施用于骨的区域中的细胞，使得所述核酸被表达以产生血管生成蛋白，从而在该区域内增强骨密度或形成。 任选地，将编码成骨蛋白的核酸施用于相同区域内的细胞，使得所述核酸被表达以产生成骨蛋白。 该方法可以用于产生具有含有编码血管生成蛋白的外源核酸的细胞的骨移植物，以及任选的含有编码成骨蛋白的核酸的细胞。 为了促进本发明的方法，本发明还涉及具有编码血管生成蛋白的核酸和编码成骨蛋白的核酸的重组病毒载体。

    Method of identifying a gene product
    65.
    发明申请
    Method of identifying a gene product 审中-公开

    公开(公告)号：US20050130219A1

    公开(公告)日：2005-06-16

    申请号：US11048574

    申请日：2005-02-01

    申请人： Miguel Carrion ,  Imre Kovesdi ,  Duncan McVey

    发明人： Miguel Carrion ,  Imre Kovesdi ,  Duncan McVey

    IPC分类号： C12N15/10 ,  C12N15/861 ,  C12Q1/68 ,  C12Q1/70

    CPC分类号： C12N15/86 ,  C12N15/1055 ,  C12N2710/10343 ,  C12Q1/6816 ,  C12Q1/6897 ,  C12Q2563/167

    摘要： The present invention provides a method of identifying a gene product. The method comprises providing a multiplicity of cells comprising a first gene product. Preferably, the first gene product is produced in the multiplicity of cells by expressing a first exogenous nucleic acid sequence encoding the first gene product. A library of second nucleic acid sequences encoding second gene products is then introduced into the multiplicity of cells. The second nucleic acid sequences are expressed in the multiplicity of cells to produce the second gene products such that the first gene product and at least one of the second gene products contact. The method further comprises causing a complex to form between the first gene product, an affinity molecule that binds the first gene product, and at least one of the second gene products, and subsequently retrieving the complex. At least one second gene product of the complex then is identified.

    Efficient purification of adenovirus
    66.
    发明授权
    Efficient purification of adenovirus 有权

    公开(公告)号：US06383795B1

    公开(公告)日：2002-05-07

    申请号：US09296962

    申请日：1999-04-22

    申请人： Miguel E. Carrión ,  Marilyn Menger ,  Imre Kovesdi

    发明人： Miguel E. Carrión ,  Marilyn Menger ,  Imre Kovesdi

    IPC分类号： C12N702

    CPC分类号： C07K14/005 ,  B01D15/345 ,  B01D15/363 ,  B01J41/20 ,  C12N7/00 ,  C12N2710/10322 ,  C12N2710/10351 ,  C12Q1/06 ,  G01N30/06 ,  G01N30/96 ,  G01N2030/625 ,  G01N2030/8813 ,  G01N2333/075

    摘要： A method of enriching a solution of an adenovirus comprising applying a mixed solution comprising an adenovirus and at least one undesired type of biomolecule to an anion exchange chromatography resin containing a binding moiety selected from the group consisting of dimethylaminopropyl, dimethylaminobutyl, dimethylaminoisobutyl, and dimethylaminopentyl and eluting the adenovirus from the chromatography resin. Also provided is a method of purifying an adenovirus from adenovirus-infected cells comprising lysing such cells, applying the lysate to a single chromatography resin, eluting the adenovirus from the chromatography resin, and collecting a fraction containing adenovirus that is substantially as pure as triple CsCl density gradient-purified adenovirus. The present method further provides a method of accurately quantifying the number of adenoviral particles in a solution of adenovirus comprising applying to and eluting from an anion exchange chromatography resin a sample solution of adenovirus, comparing the absorbance of the sample solution of adenovirus and the absorbance of a standard solution of adenovirus, and quantifying the number of adenoviral particles in the sample solution.

    Methods and compositions for preserving adenoviral vectors
    67.
    发明授权
    Methods and compositions for preserving adenoviral vectors 有权
    用于保存腺病毒载体的方法和组合物

    公开(公告)号：US06225289B1

    公开(公告)日：2001-05-01

    申请号：US09208666

    申请日：1998-12-10

    申请人： Imre Kovesdi ,  Stephen C. Ransom

    发明人： Imre Kovesdi ,  Stephen C. Ransom

    IPC分类号： A61K3170

    CPC分类号： C12N15/86 ,  A61K47/183 ,  A61K47/26 ,  A61K47/32 ,  A61K48/0091 ,  C12N2710/10343 ,  C12N2710/10351

    摘要： The present invention provides a method and composition for preserving an adenoviral vector. Both the method and composition involve the use of an adenoviral gene transfer vector, a pharmaceutically acceptable liquid carrier, and a stabilizing agent, which enables the adenoviral vector to be maintained at a temperature above 0° C. for at least 7 days.

    摘要翻译： 本发明提供了用于保存腺病毒载体的方法和组合物。 方法和组合都涉及使用腺病毒基因转移载体，药学上可接受的液体载体和稳定剂，其使腺病毒载体能够保持在0℃以上的温度下至少7天。

    MATERIALS AND METHODS FOR TREATING OCULAR-RELATED DISORDERS
    68.
    发明申请
    MATERIALS AND METHODS FOR TREATING OCULAR-RELATED DISORDERS 审中-公开
    用于治疗与眼睛有关的疾病的材料和方法

    公开(公告)号：US20070098692A1

    公开(公告)日：2007-05-03

    申请号：US11610364

    申请日：2006-12-13

    申请人： Imre Kovesdi ,  Douglas Brough ,  Lisa Wei ,  Duncan McVey

    发明人： Imre Kovesdi ,  Douglas Brough ,  Lisa Wei ,  Duncan McVey

    IPC分类号： A61K48/00

    CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/71 ,  C07K14/811 ,  C12N2710/10343 ,  C12N2799/022

    摘要： The present invention is directed to a method of prophylactically or therapeutically treating an animal for at least one ocular-related disorder, e.g., ocular neovascularization or age-related macular degeneration. The method comprises contacting an ocular cell with an expression vector comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and the same or different nucleic acid sequence encoding a neurotrophic agent. The method also can comprise contacting an ocular cell with different expression vectors, each comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and/or a nucleic acid sequence encoding a neurotrophic agent. In addition, the present invention provides a viral vector comprising a nucleic acid sequence encoding pigment epithelium-derived factor (PEDF) or a therapeutic fragment thereof.

    摘要翻译： 本发明涉及一种预防性或治疗性地治疗动物至少一种与眼部相关的疾病，例如眼睛新生血管形成或年龄相关性黄斑变性的方法。 该方法包括使眼细胞与包含编码血管生成抑制剂的核酸序列和编码神经营养剂的相同或不同核酸序列的表达载体接触。 该方法还可以包括使眼细胞与不同的表达载体接触，每种表达载体包含编码血管生成抑制剂的核酸序列和/或编码神经营养剂的核酸序列。 此外，本发明提供了包含编码色素上皮衍生因子（PEDF）的核酸序列或其治疗片段的病毒载体。

    Method of identifying a gene product
    70.
    发明授权
    Method of identifying a gene product 有权
    识别基因产物的方法

    公开(公告)号：US06861229B2

    公开(公告)日：2005-03-01

    申请号：US10336702

    申请日：2003-01-03

    申请人： Miguel E. Carrión ,  Imre Kovesdi

    发明人： Miguel E. Carrión ,  Imre Kovesdi

    IPC分类号： C12N15/10 ,  C12N15/861 ,  C12Q1/68 ,  G01N33/53 ,  C12N15/63 ,  C12N15/70 ,  C11N15/861

    CPC分类号： C12N15/86 ,  C12N15/1055 ,  C12N2710/10343 ,  C12Q1/6816 ,  C12Q1/6897 ,  C12Q2563/167

    摘要： The present invention provides a method of identifying a gene product. The method comprises providing a multiplicity of cells comprising a first gene product. Preferably, the first gene product is produced in the multiplicity of cells by expressing a first exogenous nucleic acid sequence encoding the first gene product. A library of second nucleic acid sequences encoding second gene products is then introduced into the multiplicity of cells. The second nucleic acid sequences are expressed in the multiplicity of cells to produce the second gene products such that the first gene product and at least one of the second gene products contact. The method further comprises causing a complex to form between the first gene product, an affinity molecule that binds the first gene product, and at least one of the second gene products, and subsequently retrieving the complex. At least one second gene product of the complex then is identified.

    摘要翻译： 本发明提供鉴定基因产物的方法。 该方法包括提供包含第一基因产物的多个细胞。 优选地，通过表达编码第一基因产物的第一外源核酸序列，在多种细胞中产生第一基因产物。 然后将编码第二基因产物的第二核酸序列的文库导入多个细胞。 第二核酸序列在多重细胞中表达以产生第二基因产物，使得第一基因产物和第二基因产物中的至少一个接触。 所述方法还包括使第一基因产物，结合第一基因产物的亲和分子与至少一种第二基因产物形成复合物，随后检索该复合物。 然后鉴定该复合物的至少一个第二基因产物。