公开(公告)号：US07329491B2

公开(公告)日：2008-02-12

申请号：US10962916

申请日：2004-10-12

申请人： Michael Kirchgesser ,  Frank Bergmann ,  Thomas Walter ,  Kurt Weindel ,  Ralf Zielenski ,  Emad Sarofim

发明人： Michael Kirchgesser ,  Frank Bergmann ,  Thomas Walter ,  Kurt Weindel ,  Ralf Zielenski ,  Emad Sarofim

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/1006 ,  C12N15/1003 ,  C12N15/1013

摘要： A method for purification of a nucleic acid comprising the steps of (a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aqueous buffer, (ii) salts in a high concentration, (iii) a water-miscible, non-acidic organic compound, and (iv) the nucleic acid; (b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid; (c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate; (d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid; and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.

摘要翻译： 一种用于纯化核酸的方法，包括以下步骤：（a）从含有（i）水性缓冲液，（ii）高浓度盐，（iii）水混溶性， 非酸性有机化合物，和（iv）核酸; （b）任选地用洗涤溶液洗涤具有吸附的核酸的底物; （c）与步骤（a）的组合物相比，使含有较低浓度盐的溶液与吸附的核酸接触，从而从底物解吸核酸; （d）将解离的核酸与底物分离，从而纯化核酸; 和任选地（e）从步骤（d）的溶液沉淀解吸的核酸并分离沉淀的核酸，由此进一步纯化核酸。

公开(公告)号：US20080032347A1

公开(公告)日：2008-02-07

申请号：US11777173

申请日：2007-07-12

申请人： Emad Sarofim ,  Goran Savatic

发明人： Emad Sarofim ,  Goran Savatic

IPC分类号： C12P19/34 ,  B01L7/00 ,  F25B29/00

CPC分类号： B01L7/52 ,  B01L3/5027 ,  B01L2200/147 ,  B01L2300/1827 ,  B01L2300/1844 ,  Y02P20/582

摘要： Subject of the present invention is a system comprising a cartridge and a device for heating and cooling a mixture in a controlled manner by sensing the temperature of the mixture in said cartridge with at least one sensor element, a device for heating a cartridge comprising a chamber, a method for conducting a thermal profile in a device, and a method for amplifying nucleic acids.

摘要翻译： 本发明的主题是一种系统，其包括用于通过用至少一个传感器元件感测所述盒中的混合物的温度以受控方式加热和冷却混合物的装置，用于加热包括腔室的盒的装置 ，用于在器件中进行热分布的方法，以及用于扩增核酸的方法。

公开(公告)号：US20080014615A1

公开(公告)日：2008-01-17

申请号：US11777203

申请日：2007-07-12

申请人： Emad Sarofim ,  Goran Savatic

发明人： Emad Sarofim ,  Goran Savatic

IPC分类号： C12P19/34 ,  B01J19/00 ,  C12M1/34

CPC分类号： B01L9/527 ,  B01J2219/00873 ,  B01L3/5027 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2300/185 ,  Y02P20/582

摘要： An instrument and method is provided that conveniently allows rapid amplification of nucleic acids. The instrument comprises in the following order at least one substantially flat temperature sensor element; a heat conducting substrate and a heater layer, whereby the heat conducting substrate comprises one or more channels permeable for fluid.

摘要翻译： 提供了方便地允许核酸快速扩增的仪器和方法。 仪器按以下顺序包括至少一个基本平坦的温度传感器元件; 导热衬底和加热器层，由此导热衬底包括可渗透流体的一个或多个通道。

公开(公告)号：US20080011062A1

公开(公告)日：2008-01-17

申请号：US11776367

申请日：2007-07-11

申请人： Emad Sarofim ,  Hans-Peter Wahl

发明人： Emad Sarofim ,  Hans-Peter Wahl

IPC分类号： G01N1/28

CPC分类号： B01L3/502715 ,  B01L3/5025 ,  B01L2400/0622 ,  B01L2400/0633

摘要： A analytical device comprising a body comprising a fluidic unit comprising a first chamber having an outlet opening and a first channel exiting said outlet opening, wherein said first chamber further contains an insert comprising recesses between ribs touching the walls of said first chamber and a second channel, said insert being located in a first position wherein said insert is not engaged with said outlet opening, said insert being moveable from said first position to a second position within said first chamber, wherein said insert is engaged with said outlet opening such that said second channel extends said first channel into said first chamber and in the part pointing towards said outlet opening resembles the shape of said first chamber.

摘要翻译： 一种包括主体的分析装置，包括流体单元，所述流体单元包括具有出口开口的第一室和离开所述出口开口的第一通道，其中所述第一室还包含插入件，所述插入件包括接触所述第一室的壁的肋之间的凹部， 所述插入件位于第一位置，其中所述插入件不与所述出口开口接合，所述插入件可从所述第一位置移动到所述第一室内的第二位置，其中所述插入件与所述出口开口接合，使得所述第二 通道将所述第一通道延伸到所述第一室中，并且指向所述出口的部分类似于所述第一室的形状。

公开(公告)号：US20050079535A1

公开(公告)日：2005-04-14

申请号：US10962916

申请日：2004-10-12

申请人： Michael Kirchgesser ,  Frank Bergmann ,  Thomas Walter ,  Kurt Weindel ,  Ralf Zielenski ,  Emad Sarofim

发明人： Michael Kirchgesser ,  Frank Bergmann ,  Thomas Walter ,  Kurt Weindel ,  Ralf Zielenski ,  Emad Sarofim

IPC分类号： C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/1006 ,  C12N15/1003 ,  C12N15/1013

摘要： A method for purification of a nucleic acid comprising the steps of (a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aqueous buffer, (ii) salts in a high concentration, (iii) a water-miscible, non-acidic organic compound, and (iv) the nucleic acid; ( b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid; (c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate; (d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid; and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.

摘要翻译： 一种用于纯化核酸的方法，包括以下步骤：（a）从含有（i）水性缓冲液，（ii）高浓度盐的组合物，（iii）水混溶性， 非酸性有机化合物，和（iv）核酸; （b）任选地用洗涤溶液洗涤具有吸附的核酸的底物; （c）与步骤（a）的组合物相比，使含有较低浓度盐的溶液与吸附的核酸接触，从而从底物解吸核酸; （d）将解离的核酸与底物分离，从而纯化核酸; 和任选地（e）从步骤（d）的溶液沉淀解吸的核酸并分离沉淀的核酸，由此进一步纯化核酸。