Probe Based Nucleic Acid Detection
    62.
    发明申请
    Probe Based Nucleic Acid Detection 有权
    基于探针的核酸检测

    公开(公告)号:US20130045881A1

    公开(公告)日:2013-02-21

    申请号:US13467933

    申请日:2012-05-09

    IPC分类号: G01N21/64 C40B30/04

    CPC分类号: C12Q1/6818 C12Q2525/151

    摘要: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.

    摘要翻译: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法,提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中,提供具有解链温度Tm2的调节性寡核苷酸,其包含与调节序列至少部分互补的序列片段,在PCR扩增中扩增标记的靶核酸序列 使用探针寡核苷酸作为引物的反应,检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

    METHOD AND APPARATUS FOR UPDATING PRICES FOR KEYWORD PHRASES
    64.
    发明申请
    METHOD AND APPARATUS FOR UPDATING PRICES FOR KEYWORD PHRASES 审中-公开
    用于更新关键词的价格的方法和装置

    公开(公告)号:US20120310843A1

    公开(公告)日:2012-12-06

    申请号:US13152946

    申请日:2011-06-03

    IPC分类号: G06Q99/00

    CPC分类号: G06Q10/00

    摘要: A method for updating prices for phrases may begin by receiving, at a computing device, a real-time data feed comprising a plurality of messages. The method may continue by detecting, by the computing device, a topic associated with a message in the plurality of messages using natural language processing. The topic may indicate the subject of the message. The method may continue by calculating, by the computing device, a score for the topic using temporal data associated with the message. The score may indicate the popularity of the topic. The method may continue by extracting, by the computing device, a keyword phrase from the topic. The method may conclude by determining, by the computing device, a price associated with the keyword phrase using the score.

    摘要翻译: 用于更新短语价格的方法可以开始于在计算设备处接收包括多个消息的实时数据馈送。 该方法可以通过由计算设备通过使用自然语言处理检测与多个消息中的消息相关联的主题来继续。 主题可能表示消息的主题。 该方法可以通过计算设备使用与消息相关联的时间数据来计算主题的得分来继续。 该分数可能表明该主题的受欢迎程度。 该方法可以通过由计算设备提取来自主题的关键字短语来继续。 该方法可以通过由计算设备通过使用该分数来确定与关键词短语相关联的价格来得出结论。

    FLUIDIC DEVICE
    65.
    发明申请
    FLUIDIC DEVICE 审中-公开
    流体装置

    公开(公告)号:US20120276635A1

    公开(公告)日:2012-11-01

    申请号:US13537182

    申请日:2012-06-29

    IPC分类号: C12N13/00 C12N15/02

    CPC分类号: C12M35/02 C12M23/16 C12M47/06

    摘要: A fluidic device for cell electroporation, cell lysis, and cell electrofusion based on constant DC voltage and geometric variation is provided. The fluidic device can be used with prokaryotic or eukaryotic cells. In addition, the device can be used for electroporative delivery of compounds, drugs, and genes into prokaryotic and eukaryotic cells on a microfluidic platform.

    摘要翻译: 提供了一种用于细胞电穿孔,细胞裂解和基于恒定直流电压和几何变化的细胞电融合的流体装置。 流体装置可以与原核或真核细胞一起使用。 此外,该装置可用于电化学递送化合物,药物和基因至微流体平台上的原核和真核细胞。

    Late call forwarding method in IP multimedia core network subsystem centralized service
    67.
    发明授权
    Late call forwarding method in IP multimedia core network subsystem centralized service 有权
    IP多媒体核心网子系统集中式服务中的延迟呼叫转移方法

    公开(公告)号:US08289887B2

    公开(公告)日:2012-10-16

    申请号:US12679739

    申请日:2007-11-29

    IPC分类号: H04B7/00 H04L12/66 G06F15/16

    摘要: A late call forwarding method in the IMS centralized service. During a call setting up, an ICCF receives a call request from the CSCF serving the called user, called session A, and the ICCF acquires a called user's roaming number and initiates a call to the called user. After receiving the call request, the VMSC serving the called user triggers the late call forwarding service according its determination. A CAMEL service is triggered when the VMSC initiates a call to the forwarding target. The VMSC exchanges messages with the ICCF and informs the ICCE about session associated information and the forwarding reason. When the ICCF determines that the forwarding is needed, it sends a response to the session A does not send the response message, after a telecom application server (TAS) receives the indication, the TAS initiates a regular forwarding service.

    摘要翻译: IMS集中式服务中的后期呼叫转移方法。 在呼叫建立期间,ICCF接收来自被叫用户(称为会话A)的CSCF的呼叫请求,ICCF获取被叫用户的漫游号码并发起对被叫用户的呼叫。 在接收到呼叫请求后,服务于被叫用户的VMSC根据其确定触发延迟呼叫转移服务。 当VMSC发起对转发目标的呼叫时,CAMEL服务被触发。 VMSC与ICCF交换消息,并通知ICCE会话关联信息和转发原因。 当ICCF确定需要转发时,在电信应用服务器(TAS)接收到该指示后,TAS发起定期转发业务,发送响应给会话A不发送响应消息。

    CARRIER AGGREGATION FOR EVOLVED MULTIMEDIA BROADCAST MULTICAST SERVICE ENHANCEMENT
    68.
    发明申请
    CARRIER AGGREGATION FOR EVOLVED MULTIMEDIA BROADCAST MULTICAST SERVICE ENHANCEMENT 有权
    促进多媒体广播组播服务增强的载体聚合

    公开(公告)号:US20120213130A1

    公开(公告)日:2012-08-23

    申请号:US13402845

    申请日:2012-02-22

    摘要: Carrier aggregation to enhance Evolved Multimedia Broadcast Multicast Service (eMBMS) includes transmitting unicast signaling for a unicast service on an anchor carrier to mobile entities, transmitting eMBMS signaling on a second carrier different from the anchor carrier to the mobile entities for use with the unicast signaling, and various techniques for practical application of carrier aggregation for eMBMS enhancement. In addition, allocating subframes used for MBMS on a Single Frequency Network (MBSFN) includes allocating at least a portion of one or more subframes otherwise reserved for unicast subframes on a mixed carrier to provide an increased allocation of subframes carrying MBSFN information, transmitting MBSFN signals on the increased allocation of subframes, and more detailed aspects.

    摘要翻译: 用于增强演进的多媒体广播多播服务(eMBMS)的载波聚合包括将锚定载波上的单播服务的单播信令发送到移动实体,在不同于该锚定载波的第二载波上将eMBMS信令发送到移动实体以与单播信令一起使用 ,以及用于eMBMS增强的载波聚合的实际应用的各种技术。 此外,在单频网络(MBSFN)上分配用于MBMS的子帧包括:在混合载波上分配另外保留用于单播子帧的一个或多个子帧的至少一部分,以提供携带MBSFN信息的子帧的增加分配,发送MBSFN信号 对子帧的分配增加,以及更详细的方面。

    Symbol interleaving and channel mapping device and method and mobile communication system
    69.
    发明授权
    Symbol interleaving and channel mapping device and method and mobile communication system 有权
    符号交织和信道映射设备及方法及移动通信系统

    公开(公告)号:US08243841B2

    公开(公告)日:2012-08-14

    申请号:US12175255

    申请日:2008-07-17

    IPC分类号: H04B7/02 H04L1/02

    摘要: The present invention discloses a symbol interleaving and channel mapping device and method as well as a mobile communication system. The symbol interleaving and channel mapping device is used in a transmitter of a mobile communication system, said transmitter receiving the channel state information fed back from a receiver of the mobile communication system; the symbol interleaving and channel mapping device comprises a symbol classification unit for classifying symbols according to their importance; a symbol sub-carrier mapping unit for performing a sub-carrier mapping according to the classification result of said symbol classification unit and the channel state information such that the important symbols correspond to the non deep fading sub-carrier as much as possible.

    摘要翻译: 本发明公开了一种符号交织和信道映射设备及方法以及移动通信系统。 符号交织和信道映射设备用于移动通信系统的发射机,所述发射机接收从移动通信系统的接收机反馈的信道状态信息; 符号交织和信道映射设备包括:符号分类单元,用于根据其符号对符号进行分类; 符号子载波映射单元,用于根据所述符号分类单元的分类结果和信道状态信息执行子载波映射,使得重要符号尽可能地对应于非深衰落子载波。

    Battery cover and portable electronic device using the same
    70.
    发明授权
    Battery cover and portable electronic device using the same 失效
    电池盖和使用其的便携式电子设备

    公开(公告)号:US08241782B2

    公开(公告)日:2012-08-14

    申请号:US12332535

    申请日:2008-12-11

    IPC分类号: H01M2/08 H01M2/10

    CPC分类号: H01M2/0404 H01M2/1022

    摘要: A battery cover which detachably mounted on a portable electronic device to protect the battery of the portable electronic device. The battery cover includes a main cover and a sliding cover, the sliding cover is slidably mounted on the main cover. Two opposite ends of the main cover and the sliding cover each includes at least one tab or projection protruding there from respectively. The battery cover has a simple structure, and is easy for operating. The invention also includes a portable electronic device using the battery cover.

    摘要翻译: 一种可拆卸地安装在便携式电子设备上以保护便携式电子设备的电池的电池盖。 电池盖包括主盖和滑盖,滑盖可滑动地安装在主盖上。 主盖和滑动盖的两个相对端分别包括至少一个突出在其上的突片或凸起。 电池盖结构简单,操作方便。 本发明还包括使用电池盖的便携式电子设备。