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    Probe Based Nucleic Acid Detection
    1.
    发明申请
    Probe Based Nucleic Acid Detection 有权
    基于探针的核酸检测

    公开(公告)号：US20130045881A1

    公开(公告)日：2013-02-21

    申请号：US13467933

    申请日：2012-05-09

    申请人： Kenneth J. Livak ,  Stacey N. Myers ,  Jun Wang ,  Xiaohui Wang

    发明人： Kenneth J. Livak ,  Stacey N. Myers ,  Jun Wang ,  Xiaohui Wang

    IPC分类号： G01N21/64 ,  C40B30/04

    CPC分类号： C12Q1/6818 ,  C12Q2525/151

    摘要： The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.

    摘要翻译： 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列 使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

    Probe based nucleic acid detection
    2.
    发明授权
    Probe based nucleic acid detection 有权
    基于探针的核酸检测

    公开(公告)号：US08809238B2

    公开(公告)日：2014-08-19

    申请号：US13467933

    申请日：2012-05-09

    申请人： Kenneth J. Livak ,  Stacey N. Meyers ,  Jun Wang ,  Xiaohui Wang

    发明人： Kenneth J. Livak ,  Stacey N. Meyers ,  Jun Wang ,  Xiaohui Wang

    IPC分类号： G01N21/64 ,  C40B30/04 ,  C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q2525/151

    摘要： The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.

    摘要翻译： 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列 使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

    SELF-CHECKOUT METHOD AND APPARATUS FOR UNPACKAGED PRODUCTS
    4.
    发明申请
    SELF-CHECKOUT METHOD AND APPARATUS FOR UNPACKAGED PRODUCTS 有权

    公开(公告)号：US20210383341A1

    公开(公告)日：2021-12-09

    申请号：US17151686

    申请日：2021-01-19

    申请人： Jun Wang ,  Weijun Zhang

    发明人： Jun Wang ,  Weijun Zhang

    IPC分类号： G06Q20/18 ,  G06Q20/20 ,  G06Q20/32

    摘要： A method and a vending apparatus for self-checkout unpackaged products is disclosed. The vending apparatus holds goods on one or more product trays. Load cells are mounted under each product tray and can measure the weight of the goods on the product tray in real time. A user may use a credit/debit card or an online payment app to unlock the vending apparatus and to initiate a purchase. The user may take any desired amount of goods. The apparatus automatically detects the type of goods and the weight amount taken by the user and calculates the total cost. After the user payment, the apparatus automatically resets the initial weight of the goods and is ready for the next transaction. The present invention improves the vending machine technology and expands its application to offering unpackaged products.

    Methods and apparatus for calling party number display solutions for 1xCSFB
    7.
    发明授权
    Methods and apparatus for calling party number display solutions for 1xCSFB 有权
    1xCSFB呼叫方号码显示解决方案和方法

    公开(公告)号：US09374739B2

    公开(公告)日：2016-06-21

    申请号：US13014583

    申请日：2011-01-26

    申请人： Masakazu Shirota ,  Jun Wang ,  George Cherian ,  Arvind Swaminathan ,  Srinivasan Balasubramanian ,  Ravindra Manohar Patwardhan

    发明人： Masakazu Shirota ,  Jun Wang ,  George Cherian ,  Arvind Swaminathan ,  Srinivasan Balasubramanian ,  Ravindra Manohar Patwardhan

    IPC分类号： H04W4/00 ,  H04W36/00 ,  H04W68/12

    CPC分类号： H04W36/0022 ,  H04W68/12

    摘要： Controller and method for Handover (HO) based 1×CSFB communicates Calling Party Number (CPN) to User Equipment (UE) a in a cellular communication system. A first solution provides Interworking Solution (IWS) adding CPN sent to target Base Station Controller (BSC) via Mobile Switching Center (MSC) in Interoperability Specification (IOS) HO messages. A second solution provides that IWS sends CPN with Air Interface HO message and stores the CPN until UE transition to the target Radio Access Network and acquires a traffic channel. A third solution provides that IWS receives CPN from MSC with a paging message and stores the CPN until UE transition to the target Radio Access Network and acquires a traffic channel. A fourth solution provides sending AWI message to the target Radio Access Network from the MSC. A fifth solution provides that IWS sends CPN via Feature Notification Message (FNM) data tunneled to UE before the paging message to let user decide whether to accept a call before leaving 3G/4G cellular communication.

    摘要翻译： 基于移动（HO）的1×CSFB的控制器和方法在蜂窝通信系统中向用户设备（UE）a通信呼叫方号码（CPN）a。 第一个解决方案提供互通解决方案（IWS），通过移动交换中心（MSC）在互操作性规范（IOS）HO消息中添加发送到目标基站控制器（BSC）的CPN。 第二个解决方案规定，IWS向CPN发送空中接口HO消息，并存储CPN，直到UE转移到目标无线接入网络并获取业务信道。 第三个解决方案提供IWS从MSC接收CPN寻呼消息，并存储CPN，直到UE转移到目标无线接入网络并获取业务信道。 第四个解决方案提供从MSC向目标无线电接入网发送AWI消息。 第五个解决方案提供IWS在寻呼消息之前通过隧道传输到UE的功能通知消息（FNM）数据发送CPN，以便在离开3G / 4G蜂窝通信之前决定是否接受呼叫。

    Method and system for implementing interconnection between internet protocol version 4 network and new network
    8.
    发明授权
    Method and system for implementing interconnection between internet protocol version 4 network and new network 有权
    实现互联网协议版本4网络与新网络互联的方法和系统

    公开(公告)号：US09191317B2

    公开(公告)日：2015-11-17

    申请号：US13500940

    申请日：2009-12-24

    申请人： Jun Wang

    发明人： Jun Wang

    IPC分类号： G06F15/16 ,  H04L12/56 ,  H04L29/12

    CPC分类号： H04L45/741 ,  H04L61/1511 ,  H04L61/2076 ,  H04L61/251 ,  H04L61/2517 ,  H04L61/2528 ,  H04L61/2592

    摘要： A method and system are provided for implementing intercommunication between an Internet Protocol Version 4 (IPV4) network and a new network. The new network adopts an addressing format different from that of the IPV4 network. The method includes an IPV4 network host sending a message to a new network host and a new network host sending a message to the IPV4 network host. The system includes an IPV4 network host, an intercommunication functional unit and a new network host. A method is also provided for implementing intercommunication between a first network and a second network.

    摘要翻译： 提供了一种实现互联网协议版本4（IPV4）网络和新网络之间的互通的方法和系统。 新网络采用与IPV4网络不同的寻址格式。 该方法包括向新的网络主机发送消息的IPV4网络主机和向IPV4网络主机发送消息的新网络主机。 该系统包括一个IPV4网络主机，一个互通功能单元和一个新的网络主机。 还提供了一种用于实现第一网络和第二网络之间的相互通信的方法。

    Method for manufacturing liquid crystal panel
    9.
    发明授权
    Method for manufacturing liquid crystal panel 有权
    液晶面板制造方法

    公开(公告)号：US09151980B2

    公开(公告)日：2015-10-06

    申请号：US13512590

    申请日：2012-05-02

    申请人： Jun Wang

    发明人： Jun Wang

    IPC分类号： H01L33/08 ,  G02F1/1335 ,  G02F1/13

    CPC分类号： G02F1/133514 ,  G02F2001/1316

    摘要： The present invention relates to a method for manufacturing a liquid crystal panel, which comprises steps of: forming a plurality of array thin films on a first glass substrate in turn to form an array substrate; cleaning the array substrate by ultrasound to eliminate bubbles and dirt between the array thin films. The present invention can change surface properties of each thin film of the array substrate for tightening the combination between the thin films and efficiently decreasing or minimizing bubbles and dirt between the thin films of the liquid crystal panel.

    摘要翻译： 液晶面板的制造方法技术领域本发明涉及液晶面板的制造方法，其特征在于，包括以下步骤：依次在第一玻璃基板上形成多个阵列薄膜，形成阵列基板; 通过超声清洗阵列基板以消除阵列薄膜之间的气泡和污物。 本发明可以改变阵列基板的每个薄膜的表面特性，以紧固薄膜之间的组合，并有效地降低或最小化液晶面板的薄膜之间的气泡和污物。