公开(公告)号：US20090004711A1

公开(公告)日：2009-01-01

申请号：US12189906

申请日：2008-08-12

申请人： Robert DiCosimo ,  Anna Panova ,  Jeffery Scott Thompson ,  Robert D. Fallon ,  Thomas Foo ,  F. Glenn Gallagher ,  Xu Li ,  George C. Fox ,  Joseph J. Zaher ,  Mark S. Payne ,  Daniel P. O'Keefe

发明人： Robert DiCosimo ,  Anna Panova ,  Jeffery Scott Thompson ,  Robert D. Fallon ,  Thomas Foo ,  F. Glenn Gallagher ,  Xu Li ,  George C. Fox ,  Joseph J. Zaher ,  Mark S. Payne ,  Daniel P. O'Keefe

IPC分类号： C12P7/42

CPC分类号： C07C51/48 ,  C07C51/42 ,  C07C51/487 ,  C12P7/42 ,  C07C59/06

摘要： A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.

摘要翻译： 提供从甲醛和氰化氢生产乙醇酸的方法。 更具体地，使热处理的甲醛和氰化氢反应以制备具有低浓度杂质的乙醇腈。 随后使用具有源自Acidovorax facilis 72W（ATCC 57746）的腈水解酶活性的酶催化剂将乙醇腈转化为乙醇酸铵水溶液。 使用本文所述的各种方法，从乙醇酸水溶液中以酸或盐的形式回收乙醇酸。

公开(公告)号：US20090004710A1

公开(公告)日：2009-01-01

申请号：US12189890

申请日：2008-08-12

申请人： Robert DiCosimo ,  Anna Panova ,  Jeffery Scott Thompson ,  Robert D. Fallon ,  Thomas Foo ,  F. Glenn Gallagher ,  Xu Li ,  George C. Fox ,  Joseph J. Zaher ,  Mark S. Payne ,  Daniel P. O'Keefe

发明人： Robert DiCosimo ,  Anna Panova ,  Jeffery Scott Thompson ,  Robert D. Fallon ,  Thomas Foo ,  F. Glenn Gallagher ,  Xu Li ,  George C. Fox ,  Joseph J. Zaher ,  Mark S. Payne ,  Daniel P. O'Keefe

IPC分类号： C12P7/42

CPC分类号： C07C51/48 ,  C07C51/42 ,  C07C51/487 ,  C12P7/42 ,  C07C59/06

摘要： A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.

公开(公告)号：US20090004709A1

公开(公告)日：2009-01-01

申请号：US12189884

申请日：2008-08-12

申请人： Robert DiCosimo ,  Anna Panova ,  Jeffery Scott Thompson ,  Robert D. Fallon ,  Thomas Foo ,  F. Glenn Gallagher ,  Xu Li ,  George C. Fox ,  Joseph J. Zaher ,  Mark S. Payne ,  Daniel P. O'Keefe

发明人： Robert DiCosimo ,  Anna Panova ,  Jeffery Scott Thompson ,  Robert D. Fallon ,  Thomas Foo ,  F. Glenn Gallagher ,  Xu Li ,  George C. Fox ,  Joseph J. Zaher ,  Mark S. Payne ,  Daniel P. O'Keefe

IPC分类号： C12P7/42

CPC分类号： C07C51/48 ,  C07C51/42 ,  C07C51/487 ,  C12P7/42 ,  C07C59/06

摘要： A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.

摘要翻译： 提供从甲醛和氰化氢生产乙醇酸的方法。 更具体地，使热处理的甲醛和氰化氢反应以制备具有低浓度杂质的乙醇腈。 随后使用具有源自Acidovorax facilis 72W（ATCC 57746）的腈水解酶活性的酶催化剂将乙醇腈转化为乙醇酸铵水溶液。 使用本文所述的各种方法，从乙醇酸水溶液中以酸或盐的形式回收乙醇酸。

公开(公告)号：US20090004707A1

公开(公告)日：2009-01-01

申请号：US12189869

申请日：2008-08-12

申请人： Robert DiCosimo ,  Anna Panova ,  Jeffery Scott Thompson ,  Robert D. Fallon ,  Thomas Foo ,  F. Glenn Gallagher ,  Xu Li ,  George C. Fox ,  Joseph J. Zaher ,  Mark S. Payne ,  Daniel P. O'Keefe

发明人： Robert DiCosimo ,  Anna Panova ,  Jeffery Scott Thompson ,  Robert D. Fallon ,  Thomas Foo ,  F. Glenn Gallagher ,  Xu Li ,  George C. Fox ,  Joseph J. Zaher ,  Mark S. Payne ,  Daniel P. O'Keefe

IPC分类号： C12P7/42

CPC分类号： C07C51/48 ,  C07C51/42 ,  C07C51/487 ,  C12P7/42 ,  C07C59/06

摘要： A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.

摘要翻译： 提供从甲醛和氰化氢生产乙醇酸的方法。 更具体地，使热处理的甲醛和氰化氢反应以制备具有低浓度杂质的乙醇腈。 随后使用具有源自Acidovorax facilis 72W（ATCC 57746）的腈水解酶活性的酶催化剂将乙醇腈转化为乙醇酸铵水溶液。 使用本文所述的各种方法，从乙醇酸水溶液中以酸或盐的形式回收乙醇酸。

公开(公告)号：US07405064B2

公开(公告)日：2008-07-29

申请号：US11601156

申请日：2006-11-16

申请人： Mark S. Payne ,  Robert DiCosimo ,  John E. Gavagan ,  Robert D. Fallon

发明人： Mark S. Payne ,  Robert DiCosimo ,  John E. Gavagan ,  Robert D. Fallon

IPC分类号： C12P13/00 ,  C12P13/02 ,  C07H21/04 ,  C12N9/80 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N9/88 ,  C07K14/195 ,  C12P13/02 ,  C12P17/12 ,  C12P21/02

摘要： The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitriles to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.

摘要翻译： 本发明涉及编码来自Comamonas睾丸酮5-MGAM-4D的腈水合酶（NHase）或酰胺酶（Am）的基因的分离，测序和重组表达，其中NH酶可用于催化腈水合至相应的 酰胺，并且酰胺酶可用于将酰胺水解成相应的羧酸。 还提供了转化宿主细胞，其含有用于从睾丸酮睾酮5-MGAM-4D中表达腈水合酶或酰胺酶的多核苷酸。

公开(公告)号：US07285410B2

公开(公告)日：2007-10-23

申请号：US11585036

申请日：2006-10-23

申请人： Michael G. Bramucci ,  Robert DiCosimo ,  Sarita Chauhan ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

发明人： Michael G. Bramucci ,  Robert DiCosimo ,  Sarita Chauhan ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

IPC分类号： C12N1/20 ,  C12N9/78 ,  C12N9/88 ,  C12N15/00 ,  C12Q1/34 ,  C07K14/00 ,  C07H21/04

CPC分类号： C12N9/88 ,  C12N9/80 ,  C12P7/40 ,  C12P41/006

摘要： A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.

摘要翻译： 已经开发了一种由（E，Z）-2-甲基-2-丁烯腈（2M 2 BN）的混合物通过区域选择性制备（E） - 和（Z）-2-甲基-2-丁烯酸（2M 2 BA）的方法 使用具有腈水解酶活性或腈水合酶和酰胺酶活性的组合的酶催化剂将（E）-2M2BN水解成（E）-2-甲基-2-丁烯酸（2M2BA）。 该方法提供高产率而不显着转化（Z）-2M2BN至（Z）-2M2BA。 （E）-2M2BN至（E）-2M2BA的区域选择性水解使（E）-2M2BA与（Z）-2M2BN或（Z）-2-甲基-2-丁烯酰胺（2M2BAm）的容易分离成为可能， 随后将（Z）-2M2BN或（Z）-2M2BAm转化为（Z）-2M2BA。

公开(公告)号：US07285406B2

公开(公告)日：2007-10-23

申请号：US11601230

申请日：2006-11-16

申请人： Mark S. Payne ,  Robert DiCosimo ,  John E. Gavagan ,  Robert D. Fallon

发明人： Mark S. Payne ,  Robert DiCosimo ,  John E. Gavagan ,  Robert D. Fallon

IPC分类号： C12N9/00 ,  C12N9/80 ,  C12N9/06 ,  C12N15/74 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/88 ,  C07K14/195 ,  C12P13/02 ,  C12P17/12 ,  C12P21/02

摘要： The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitrites to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.

公开(公告)号：US07220562B2

公开(公告)日：2007-05-22

申请号：US11338471

申请日：2006-01-24

申请人： Michael G. Bramucci ,  Robert DiCosimo ,  Sarita Chauhan ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

发明人： Michael G. Bramucci ,  Robert DiCosimo ,  Sarita Chauhan ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

IPC分类号： C12P13/00 ,  C12P7/40 ,  C12Q1/34 ,  C12N11/14 ,  C12N11/02 ,  C12N1/20 ,  C12N9/78 ,  C12N15/00 ,  C07H21/04 ,  C07K14/00

CPC分类号： C12N9/88 ,  C12N9/80 ,  C12P7/40 ,  C12P41/006

摘要： A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.

公开(公告)号：US06383786B1

公开(公告)日：2002-05-07

申请号：US09948535

申请日：2001-09-07

申请人： Sarita Chauhan ,  Robert Dicosimo ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

发明人： Sarita Chauhan ,  Robert Dicosimo ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

IPC分类号： C12P740

CPC分类号： C12P7/42 ,  C12N9/78

摘要： The present invention relates to a method for producing &agr;-hydroxy acids using an enzyme catalyst having nitrilase activity. More specifically, the invention pertains to use of Acidovorax facilis 72W (ATCC 55746) nitrilase to hydrolyze glycolonitrile to glycolic acid. Glycolonitrile is reacted in an aqueous mixture with a catalyst having Acidovorax facilis 72W nitrilase activity to give glycolic acid selectively, and at high concentration and high yield.

摘要翻译： 本发明涉及使用具有腈水解酶活性的酶催化剂生产α-羟基酸的方法。 更具体地，本发明涉及使用Acidovorax facilis 72W（ATCC 55746）腈水解酶将乙醇腈水解成乙醇酸。 乙醇腈在含水混合物中与具有酸性酵母72W腈水解酶活性的催化剂反应，选择性地得到乙醇酸，并以高浓度和高产率。

公开(公告)号：US5728556A

公开(公告)日：1998-03-17

申请号：US615365

申请日：1996-03-14

申请人： Robert DiCosimo ,  Barry Stieglitz ,  Robert D. Fallon

发明人： Robert DiCosimo ,  Barry Stieglitz ,  Robert D. Fallon

IPC分类号： C12P13/02

CPC分类号： C12P13/02 ,  Y10S435/877

摘要： Applicants have provided methods for obtaining aliphatic omega-cyanocaboximides of Formula I NC--CH(R.sub.1)(CH).sub.n CH(R.sub.2)C(O)NH.sub.2 wherein n=1-8 and R.sub.1 or R.sub.2 are either H or CH.sub.3, from dinitriles of Formula II NC--CH(R.sub.1)(CH).sub.n CH(R.sub.2)CN wherein n=1-8 and R.sub.1 or R.sub.2 are either H or CH.sub.3, using biocatalysts which have regioselective nitrile hydratase activity and which are derived from members of the bacterial species Pseudomonas putida.

摘要翻译： 申请人提供了获得式I的NC-CH（R 1）（CH）n CH（R 2）C（O）NH 2，n = 1-8和R 1或R 2为H或CH 3的脂肪族ω-氰基邻苯二甲酰亚胺的方法， II NC-CH（R1）（CH）nCH（R2）CN，其中n = 1-8，R1或R2是H或CH3，使用具有区域选择性腈水合酶活性的生物催化剂，其衍生自恶臭假单胞菌（Pseudomonas putida）的成员 。