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公开(公告)号：US07198926B2
公开(公告)日：2007-04-03
申请号：US10431965
申请日：2003-05-08
申请人： Michael G. Bramucci , Robert Dicosimo , John E. Gavagan , Mark S. Payne , Robert D. Fallon , Sarita Chauhan
发明人： Michael G. Bramucci , Robert Dicosimo , John E. Gavagan , Mark S. Payne , Robert D. Fallon , Sarita Chauhan
IPC分类号： C12P13/00 , C12P7/40 , C12Q1/34 , C12N11/14 , C12N11/02 , C12N1/20 , C12N9/78 , C12N15/00 , C07H21/04 , C07K14/00
CPC分类号： C12N9/88 , C12N9/80 , C12P7/40 , C12P41/006
摘要： A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.
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公开(公告)号：US07285410B2
公开(公告)日：2007-10-23
申请号：US11585036
申请日：2006-10-23
申请人： Michael G. Bramucci , Robert DiCosimo , Sarita Chauhan , Robert D. Fallon , John E. Gavagan , Mark S. Payne
发明人： Michael G. Bramucci , Robert DiCosimo , Sarita Chauhan , Robert D. Fallon , John E. Gavagan , Mark S. Payne
CPC分类号： C12N9/88 , C12N9/80 , C12P7/40 , C12P41/006
摘要： A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.
摘要翻译： 已经开发了一种由（E，Z）-2-甲基-2-丁烯腈（2M 2 BN）的混合物通过区域选择性制备（E） - 和（Z）-2-甲基-2-丁烯酸（2M 2 BA）的方法 使用具有腈水解酶活性或腈水合酶和酰胺酶活性的组合的酶催化剂将（E）-2M2BN水解成（E）-2-甲基-2-丁烯酸（2M2BA）。 该方法提供高产率而不显着转化（Z）-2M2BN至（Z）-2M2BA。 （E）-2M2BN至（E）-2M2BA的区域选择性水解使（E）-2M2BA与（Z）-2M2BN或（Z）-2-甲基-2-丁烯酰胺（2M2BAm）的容易分离成为可能， 随后将（Z）-2M2BN或（Z）-2M2BAm转化为（Z）-2M2BA。
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公开(公告)号：US07220562B2
公开(公告)日：2007-05-22
申请号：US11338471
申请日：2006-01-24
申请人： Michael G. Bramucci , Robert DiCosimo , Sarita Chauhan , Robert D. Fallon , John E. Gavagan , Mark S. Payne
发明人： Michael G. Bramucci , Robert DiCosimo , Sarita Chauhan , Robert D. Fallon , John E. Gavagan , Mark S. Payne
IPC分类号： C12P13/00 , C12P7/40 , C12Q1/34 , C12N11/14 , C12N11/02 , C12N1/20 , C12N9/78 , C12N15/00 , C07H21/04 , C07K14/00
CPC分类号： C12N9/88 , C12N9/80 , C12P7/40 , C12P41/006
摘要： A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.
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4.发明授权公开(公告)号：US06455730B1
公开(公告)日：2002-09-24
申请号：US09632419
申请日：2000-08-04
IPC分类号： C07C5108
摘要： This invention relates to a process for preparing dicarboxylic acid monoesters from cyanocarboxylic acid esters. More particularly, using the nitrilase from Acidovorax facilis 72W (ATCC 55745) in various forms as an enzyme catalyst, an aliphatic or aromatic cyanocarboxylic acid ester is converted to the corresponding dicarboxylic acid monoester with high chemoselectivity at 100% conversion. An embodiment of the invention also provides a process for obtaining high chemoselectively and high regioselectivity.
摘要翻译： 本发明涉及由氰基羧酸酯制备二羧酸单酯的方法。 更具体地，使用来自Acidovorax facilis 72W（ATCC 55745）的腈水解酶以各种形式作为酶催化剂，将脂族或芳族氰基羧酸酯在100％转化率下转化为具有高化学选择性的相应的二羧酸单酯。 本发明的实施方案还提供了获得高化学选择性和高区域选择性的方法。
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5.发明授权Isolation and expression of a gene for a nitrilase from Acidovorax faclilis 72W 失效从酸性酵母72W中分离和表达腈水解酶基因公开(公告)号：US07056709B2
公开(公告)日：2006-06-06
申请号：US10376653
申请日：2003-02-27
IPC分类号： C12P7/40 , C07H21/04 , C12Q1/68 , C07D211/60
CPC分类号： C12P13/00 , C12N9/78 , C12P7/40 , C12P13/002 , C12P13/02
摘要： Recombinant microbial strains are provided that express nitrilase enzyme and are useful as biocatalysts for the hydrolysis of nitrile-containing substrates. The recombinant cells are transformed with a foreign gene isolated from Acidovorax facilis 72W encoding a thermostable nitrilase enzyme that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the deduced amino acid sequence encoded by the nitrilase gene are provided.
摘要翻译： 提供重组微生物菌株，其表达腈水解酶，并且可用作用于含腈底物水解的生物催化剂。 将重组细胞用分离自Acidovorax facilis 72W的外源基因转化，其编码热稳定的腈水解酶，其在温和的反应条件下催化含腈底物水解成羧酸。 提供了腈水解酶基因的核苷酸序列和由腈水解酶基因编码的推导的氨基酸序列。
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6.发明授权Isolation and expression of a gene for a nitrilase from Acidovorax facilis 72W 有权从Acidovorax facilis 72W中分离和表达腈水解酶基因公开(公告)号：US06870038B2
公开(公告)日：2005-03-22
申请号：US09823373
申请日：2001-03-30
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/78 , C12P7/40 , C12P13/00 , C12P17/10 , C12R1/19 , C07H21/02 , C07H21/04 , C12Q1/68
CPC分类号： C12P13/00 , C12N9/78 , C12P7/40 , C12P13/002 , C12P13/02
摘要： Recombinant microbial strains are provided that express nitrilase enzyme and are useful as biocatalysts for the hydrolysis of nitrile-containing substrates. The recombinant cells are transformed with a foreign gene isolated from Acidovorax facilis 72W encoding a thermostable nitrilase enzyme that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the deduced amino acid sequence encoded by the nitrilase gene are provided.
摘要翻译： 提供重组微生物菌株，其表达腈水解酶，并且可用作用于含腈底物水解的生物催化剂。 将重组细胞用分离自Acidovorax facilis 72W的外源基因转化，其编码热稳定的腈水解酶，其在温和的反应条件下催化含腈底物水解成羧酸。 提供了腈水解酶基因的核苷酸序列和由腈水解酶基因编码的推导的氨基酸序列。
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7.发明授权公开(公告)号：US06416980B1
公开(公告)日：2002-07-09
申请号：US09791929
申请日：2001-02-23
IPC分类号： C12P740
摘要： The present invention relates to a method for producing &agr;-hydroxy acids using an enzyme catalyst having nitrilase activity. More specifically, the invention pertains to use of Acidovorax facilis 72W (ATCC 55746) nitrilase to hydrolyze glycolonitrile to glycolic acid. Glycolonitrile is reacted in an aqueous mixture with a catalyst having Acidovorax facilis 72W nitrilase activity to give glycolic acid selectively, and at high concentration and high yield.
摘要翻译： 本发明涉及使用具有腈水解酶活性的酶催化剂生产α-羟基酸的方法。 更具体地，本发明涉及使用Acidovorax facilis 72W（ATCC 55746）腈水解酶将乙醇腈水解成乙醇酸。 乙醇腈在含水混合物中与具有酸性酵母72W腈水解酶活性的催化剂反应，选择性地得到乙醇酸，并以高浓度和高产率。
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8.发明授权Method for producing &agr;-hydroxy acid from &agr;-hydroxy nitrile using nitrilase 失效使用腈水解酶从α-羟基腈生产α-羟基酸的方法公开(公告)号：US06383786B1
公开(公告)日：2002-05-07
申请号：US09948535
申请日：2001-09-07
IPC分类号： C12P740
摘要： The present invention relates to a method for producing &agr;-hydroxy acids using an enzyme catalyst having nitrilase activity. More specifically, the invention pertains to use of Acidovorax facilis 72W (ATCC 55746) nitrilase to hydrolyze glycolonitrile to glycolic acid. Glycolonitrile is reacted in an aqueous mixture with a catalyst having Acidovorax facilis 72W nitrilase activity to give glycolic acid selectively, and at high concentration and high yield.
摘要翻译： 本发明涉及使用具有腈水解酶活性的酶催化剂生产α-羟基酸的方法。 更具体地，本发明涉及使用Acidovorax facilis 72W（ATCC 55746）腈水解酶将乙醇腈水解成乙醇酸。 乙醇腈在含水混合物中与具有酸性酵母72W腈水解酶活性的催化剂反应，选择性地得到乙醇酸，并以高浓度和高产率。
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9.发明授权Nucleic acid fragments encoding nitrile hydratase and amidase enzymes from Comamonas testosteroni 5-MGAM-4D and recombinant organisms expressing those enzymes useful for the production of amides and acids 失效从Comamonas testosteroni 5-MGAM-4D编码腈水合酶和酰胺酶的核酸片段和表达可用于产生酰胺和酸的酶的重组生物公开(公告)号：US07294493B2
公开(公告)日：2007-11-13
申请号：US11601186
申请日：2006-11-16
CPC分类号： C12N9/88 , C07K14/195 , C12P13/02 , C12P17/12 , C12P21/02
摘要： The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitriles to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.
摘要翻译： 本发明涉及编码来自Comamonas睾丸酮5-MGAM-4D的腈水合酶（NHase）或酰胺酶（Am）的基因的分离，测序和重组表达，其中NH酶可用于催化腈水合至相应的 酰胺，并且酰胺酶可用于将酰胺水解成相应的羧酸。 还提供了转化宿主细胞，其含有用于从睾丸酮睾酮5-MGAM-4D中表达腈水合酶或酰胺酶的多核苷酸。
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10.发明授权Nucleic acid fragments encoding nitrile hydratase and amidase enzymes from Comamonas testosteroni 5-MGAM-4D and recombinant organisms expressing those enzymes useful for the production of amides and acids 失效从Comamonas testosteroni 5-MGAM-4D编码腈水合酶和酰胺酶的核酸片段和表达可用于产生酰胺和酸的酶的重组生物公开(公告)号：US07405064B2
公开(公告)日：2008-07-29
申请号：US11601156
申请日：2006-11-16
CPC分类号： C12N9/88 , C07K14/195 , C12P13/02 , C12P17/12 , C12P21/02
摘要： The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitriles to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.
摘要翻译： 本发明涉及编码来自Comamonas睾丸酮5-MGAM-4D的腈水合酶（NHase）或酰胺酶（Am）的基因的分离，测序和重组表达，其中NH酶可用于催化腈水合至相应的 酰胺，并且酰胺酶可用于将酰胺水解成相应的羧酸。 还提供了转化宿主细胞，其含有用于从睾丸酮睾酮5-MGAM-4D中表达腈水合酶或酰胺酶的多核苷酸。
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