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133 条记录 (耗时 0.035 秒)

    Modified green fluorescent proteins
    61.
    发明授权
    Modified green fluorescent proteins 失效
    改良的绿色荧光蛋白

    公开(公告)号：US06319669B1

    公开(公告)日：2001-11-20

    申请号：US08727452

    申请日：1997-03-20

    申请人： Roger Y. Tsien ,  Roger Heim

    发明人： Roger Y. Tsien ,  Roger Heim

    IPC分类号： C12Q168

    CPC分类号： C07K14/43595 ,  C12N15/65 ,  G01N33/533 ,  G01N2333/43595 ,  Y10S530/855

    摘要： Modifications in the sequence of Aequorea wild-type GFP provide products having markedly different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.

    摘要翻译： Aequorea野生型GFP序列的修饰提供了来自野生型GFP相应产物的产生明显不同的激发和发射光谱的产物。 在一类修饰中，衍生自经修饰的GFP的产物表现出与用野生型GFP产生的产物观察到的两个主要激发峰的比例的变化。 在另一类中，衍生自经修饰的GFP的产物在比来自野生型GFP的相应产物更短的波长处发荧光。 在另一类修饰中，衍生自经修饰的GFP的产物相对于衍生自野生型GFP的产物仅显示单个激发峰和增强的发射。

    Light-triggered indicators that memorize analyte concentrations
    63.
    发明授权
    Light-triggered indicators that memorize analyte concentrations 失效
    记录分析物浓度的光触发指标

    公开(公告)号：US06180411B2

    公开(公告)日：2001-01-30

    申请号：US09134668

    申请日：1998-07-30

    申请人： Roger Y. Tsien ,  Stephen R. Adams

    发明人： Roger Y. Tsien ,  Stephen R. Adams

    IPC分类号： G01N33532

    CPC分类号： C07D307/82 ,  G01N33/84 ,  Y10S436/805

    摘要： A new class of optical indicators which are capable of memorizing and preserving the spatial localization of intracellular analytes in a time resolved manner is described. The compounds comprise a chromophore carrying a photolabile group capable of undergoing an irreversible and detectable chemical transformation upon irradiation by light. The chromophore is linked to a binding site capable of binding an analyte, wherein binding of the analyte to the binding site alters an optical property of the chromophore, thus altering the ability of the photolabile group to undergo the chemical transformation. Methods and kits for memorizing the spatial localization of the analytes are also described.

    摘要翻译： 描述了能够以时间分辨方式记忆和保留细胞内分析物的空间定位的新类型的光学指示器。 所述化合物包含携带光照不稳定基团的发色团，所述光不稳定基团能在光照射时经历不可逆和可检测的化学转化。 发色团连接到能够结合分析物的结合位点，其中分析物与结合位点的结合改变发色团的光学性质，从而改变光不稳定组进行化学转化的能力。 还描述了用于记忆分析物的空间定位的方法和试剂盒。

    Modified green fluorescent proteins
    67.
    发明授权
    Modified green fluorescent proteins 失效
    改良的绿色荧光蛋白

    公开(公告)号：US5625048A

    公开(公告)日：1997-04-29

    申请号：US337915

    申请日：1994-11-10

    申请人： Roger Y. Tsien ,  Roger Heim

    发明人： Roger Y. Tsien ,  Roger Heim

    IPC分类号： C12N15/09 ,  C07K14/435 ,  C12N5/10 ,  C12N15/12 ,  C12N15/65 ,  C12P21/04 ,  C12Q1/25 ,  C12Q1/68 ,  G01N33/533 ,  C07H21/04

    CPC分类号： C07K14/43595 ,  C12N15/65 ,  G01N33/533 ,  G01N2333/43595 ,  Y10S530/855

    摘要： Modifications in the sequence of Aequorea wild-type GFP provide products having markedly different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.

    摘要翻译： Aequorea野生型GFP序列的修饰提供了来自野生型GFP相应产物的产生明显不同的激发和发射光谱的产物。 在一类修饰中，衍生自经修饰的GFP的产物表现出与用野生型GFP产生的产物观察到的两个主要激发峰的比例的变化。 在另一类中，衍生自经修饰的GFP的产物在比来自野生型GFP的相应产物更短的波长处发荧光。 在另一类修饰中，衍生自经修饰的GFP的产物相对于衍生自野生型GFP的产物仅显示单个激发峰和增强的发射。

    Azide-containing chelators whose affinity for calcium ion is decreased by illumination
    68.
    发明授权
    Azide-containing chelators whose affinity for calcium ion is decreased by illumination 失效
    含钙螯合剂，其对钙离子的亲和力通过照射而降低

    公开(公告)号：US5602258A

    公开(公告)日：1997-02-11

    申请号：US968002

    申请日：1992-10-23

    申请人： Roger Y. Tsien ,  Stephen R. Adams

    发明人： Roger Y. Tsien ,  Stephen R. Adams

    IPC分类号： C07D277/28 ,  C07D413/04 ,  C07D413/14 ,  C07D263/30

    CPC分类号： C07D413/04 ,  C07D413/14

    摘要： The present invention relates to a group of organic chelators whose affinity for calcium ion in solution is decreased by electromagnetic radiation. Specifically, the chelators are related to fura-2 and utilize the addition of an azide group to the 3-position of the benzofuron ring of a fura-2 type structure. Photolysis of the azide group causes the calcium ion affinity to decrease 100 to 1000 fold. These chelators when incorporated into rat fibroblasts either by microinjection or by incubation as the membrane-permeable, enzymatically-labile esters and flash-photolyzed cause large increases in intracellular free calcium ion. These chelators are used to generate controlled fast elevation of intracellular free calcium ion concentration to mimic or modulate a number of important cellular responses, especially in nerve or muscle.

    摘要翻译： 本发明涉及通过电磁辐射降低溶液中对钙离子的亲和力的一组有机螯合剂。 具体地说，螯合剂与fura-2相关，并且利用向fura-2型结构的苯并呋喃环的3位添加叠氮基。 叠氮化物基团的光解使钙离子亲和力降低100〜1000倍。 当这些螯合剂通过显微注射或通过温育作为膜可渗透的酶不稳定的酯并且闪光光解引入大鼠成纤维细胞时，引起细胞内游离钙离子的大量增加。 这些螯合剂用于产生受控的快速升高的细胞内游离钙离子浓度以模拟或调节许多重要的细胞反应，特别是在神经或肌肉中。

    Scanning confocal microscope providing a continuous display
    69.
    发明授权
    Scanning confocal microscope providing a continuous display 失效
    扫描共焦显微镜提供连续显示

    公开(公告)号：US5283433A

    公开(公告)日：1994-02-01

    申请号：US958038

    申请日：1992-10-05

    申请人： Roger Y. Tsien

    发明人： Roger Y. Tsien

    IPC分类号： G01N21/64 ,  G02B21/00 ,  H01J3/14

    CPC分类号： G02B21/008 ,  G02B21/0076

    摘要： A scanning confocal microscope scans a sample with an incident beam of radiation, in a raster scan pattern, causing the sample to fluoresce and emit visible radiation in at least two wavelengths. A portion of the fluorescent light retraces a portion of the path optical of incident beam, to a dichroic mirror that separates it from the incident beam for detection by a pair of photomultiplier tubes. A data processor accumulates digital data from the photomultiplier tubes to form a succession of image data frames of the sample being scanned, in the two wavelengths. Image data for a selected number of frames in each wavelength is averaged and then recorded on a single track of a recording system, in an alternating fashion with averaged data for the other wavelength. In addition, a ratio of the averaged data for the two wavelengths is delivered to a video display.

    摘要翻译： 扫描共焦显微镜以光栅扫描图案中的入射光束扫描样品，导致样品在至少两个波长内发出可见光。 荧光的一部分将入射光束的光路的一部分返回到分色镜，该分色镜将其与入射光束分离，以便由一对光电倍增管检测。 数据处理器累积来自光电倍增管的数字数据，以在两个波长内形成扫描样本的一系列图像数据帧。 对每个波长中所选择的帧数的图像数据进行平均化，然后以与其他波长的平均数据交替的方式记录在记录系统的单个轨道上。 此外，两个波长的平均数据的比率被传送到视频显示器。

    Fluorescent indicator dyes for alkali metal cations
    70.
    发明授权
    Fluorescent indicator dyes for alkali metal cations 失效
    用于碱金属阳离子的荧光指示剂染料

    公开(公告)号：US5134232A

    公开(公告)日：1992-07-28

    申请号：US391879

    申请日：1989-08-09

    申请人： Roger Y. Tsien ,  Akwasi Minta

    发明人： Roger Y. Tsien ,  Akwasi Minta

    IPC分类号： C07D273/00 ,  C07D273/08 ,  C07D413/04 ,  C07D413/14 ,  C07D491/04 ,  G01N33/84

    CPC分类号： C07D273/00 ,  C07D413/04 ,  C07D413/14 ,  C07D491/04 ,  G01N33/84

    摘要： The invention discloses fluorescent chelator compounds that are especially useful to monitor and measure cytosolic concentrations of alkali metal cations such as Na.sup.+, K.sup.+, and Li.sup.+. The new compounds are comprised of: (1) crown ethers (that may or may not have substituent groups attached to the core carbons, but will always contain at least one core nitrogen) that are linked via the core nitrogen(s) to at least one (2) fluorophore that contains an additional heteroaromatic liganding center. In the currently preferred dye, SBFI, the core compound is crown ether 1,7-diaza-4,10,13-trioxacyclopentadecane and the heteroaromatic fluorophores are benzofurans that are linked to isophthalate groups. Selectivities for Na.sup.+ over K.sup.+ of about 20 are observed, resulting in effective dissociation constants for Na.sup.+ of about 20 mM against a background of 120 mM K.sup.+. Increasing [Na.sup.+ ] increases the ratio of excitation efficiency at 330-345 nm to that at 370-390 nm with emission collected at 450-550 nm, so that ratio fluorometry and imaging work at the same wavelengths as used with the well-known Ca.sup.2+ indicator fura-2. If the macrocyclic ring is increased in size to a 1,10-diaza-4,7,13,16-tetraoxacyclooctadecane, the chelators become selective for K.sup.+ over Na.sup.+. If the ring is decreased in size, for example to a 1,7-diaza-4,10-dioxacyclododecane, the chelators become selective for Li.sup.+ over Na.sup.+.

    摘要翻译： 本发明公开了特别可用于监测和测量碱金属阳离子如Na +，K +和Li +的胞质浓度的荧光螯合剂化合物。 新化合物包括：（1）冠醚（其可以具有或不具有连接到核心碳上的取代基但总是含有至少一个核心氮），其通过核心氮至少连接至少 一个（2）荧光团，其中包含一个额外的杂芳族结合中心。 在目前优选的染料中，SBFI，核心化合物是冠醚1,7二氮杂-4,10,13-三氧杂环十五烷，杂芳族荧光团是与间苯二酸酯基团连接的苯并呋喃。 观察到Na +在K +上约20的选择性，导致对于120mM K +背景的Na +约20mM的有效解离常数。 增加[Na +]增加了在330-345nm处的激发效率与在370-390nm的激发效率的比率，其中在450-550nm收集的发射，使得比例荧光测定和成像工作在与公知的Ca2 + 指标fura-2。 如果大环的大小增加到1,10-二氮杂-4,7,13,16-四氧杂环十八烷，则螯合剂对于Na +而言对K +变得选择性。 如果环尺寸减小，例如减小到1,7-diaza-4,10-二氧杂环十二烷，那么螯合剂对Na +的选择性变得更高。