L-glutamic acid-producing bacterium and method for producing L-glutamic acid
    63.
    发明授权
    L-glutamic acid-producing bacterium and method for producing L-glutamic acid 有权
    L-谷氨酸生产菌和L-谷氨酸的生产方法

    公开(公告)号:US06682912B2

    公开(公告)日:2004-01-27

    申请号:US09737580

    申请日:2000-12-18

    IPC分类号: C12P1314

    摘要: A method for producing L-glutamic acid which comprises culturing a microorganism belonging to the genus Klebsiella, Erwinia or Pantoea and having an ability to produce L-glutamic acid in a culture medium, and collecting produced L-glutamic acid from the culture medium. The microbial strain used is preferably a strain which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, or a strain which increase in an activity of an enzyme catalyzing a reaction for L-glutamic acid biosynthesis.

    摘要翻译: 一种L-谷氨酸的制造方法,其特征在于,在培养基中培养属于克雷伯氏菌属,欧文氏菌属或泛菌属的微生物,并具有生产L-谷氨酸的能力,从培养基中收集L-谷氨酸。 所用的微生物菌株优选是一种在L-谷氨酸生物合成途径中催化分支反应并产生除L-谷氨酸以外的化合物的酶的活性降低或缺乏的菌株或增加 催化L-谷氨酸生物合成反应的酶的活性。

    Temperature sensitive plasmid for coryneform bacteria
    65.
    发明授权
    Temperature sensitive plasmid for coryneform bacteria 有权
    棒状细菌的温度敏感质粒

    公开(公告)号:US06303383B1

    公开(公告)日:2001-10-16

    申请号:US09521668

    申请日:2000-03-08

    IPC分类号: C12N1574

    CPC分类号: C12N15/77

    摘要: A coryneform bacterium in which a DNA fragment is incorporated into its chromosome is prepared by (a) obtaining a recombinant plasmid through ligation of a DNA fragment having a sequence homologous to a gene present on a chromosome of a coryneform bacterium to a plasmid that has a wild-type replication control region segment of a particular nucleotide sequence including a mutation and is autonomously replicable in a coryneform bacterium cell at a culture temperature lower than 31° C. but not autonomously replicable in the cell at a temperature of 31° C. or higher, (b) introducing the recombinant plasmid into the coryneform bacterium cell, (c) culturing the bacterium at a temperature of 31° C. or higher, (d) causing homologous recombination between the DNA fragment and the gene present on the chromosome of the coryneform bacterium and having a sequence homologous to the DNA fragment, and (e) selecting a coryneform bacterium in which the DNA fragment is incorporated into its chromosome. According to the present invention, there is provided a method for efficiently modifying genetic traits of a host in a short period of time by obtaining a temperature sensitive plasmid from a plasmid not exhibiting homology with already reported temperature sensitive plasmids or not exhibiting incompatibility therewith.

    摘要翻译: 通过(a)通过将具有与存在于棒状杆菌型细菌的染色体上的基因同源的序列的DNA片段连接到具有下列基因的质粒来获得重组质粒来制备将DNA片段并入其染色体中的棒状细菌: 包含突变的特定核苷酸序列的野生型复制控制区段,并且在棒状细菌细胞中,在低于31℃的培养温度下是可自主复制的,但在31℃的温度下不能在细胞中自主复制,或 (b)将重组质粒引入棒状细菌细胞中,(c)在31℃或更高的温度下培养细菌,(d)使DNA片段与存在于染色体上的基因之间的同源重组 所述棒状细菌具有与所述DNA片段同源的序列,和(e)选择其中所述DNA片段并入其染色体中的棒状细菌 e。 根据本发明,提供了一种通过从未报道的温度敏感性质粒不显示同源性的质粒或不表现出不相容性的质粒获得温度敏感性质粒来在短时间内高效地修饰宿主遗传性状的方法。

    L-glutamic acid-producing bacterium and method for producing L-glutamic acid
    66.
    发明授权
    L-glutamic acid-producing bacterium and method for producing L-glutamic acid 有权
    L-谷氨酸生产菌和L-谷氨酸的生产方法

    公开(公告)号:US06197559B1

    公开(公告)日:2001-03-06

    申请号:US09271437

    申请日:1999-03-18

    IPC分类号: C12P1314

    摘要: A method for producing L-glutamic acid which comprises culturing a microorganism belonging to the genus Klebsiella, Erwinia or Pantoea and having an ability to produce L-glutamic acid in a culture medium, and collecting produced L-glutamic acid from the culture medium. The microbial strain used is preferably a strain which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, or a strain which increase in an activity of an enzyme catalyzing a reaction for L-glutamic acid biosynthesis.

    摘要翻译: 一种L-谷氨酸的制造方法,其特征在于,在培养基中培养属于克雷伯氏菌属,欧文氏菌属或泛菌属的微生物,并具有生产L-谷氨酸的能力,从培养基中收集L-谷氨酸。 所使用的微生物菌株优选是一种在L-谷氨酸生物合成途径中催化分支反应并产生除L-谷氨酸以外的化合物的酶的活性降低或缺乏的菌株或增加 催化L-谷氨酸生物合成反应的酶的活性。