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    Methods for sequencing with single frequency detection
    63.
    发明授权
    Methods for sequencing with single frequency detection 有权

    公开(公告)号：US11584963B2

    公开(公告)日：2023-02-21

    申请号：US16945173

    申请日：2020-07-31

    Applicant: ULTIMA GENOMICS, INC.

    Inventor： Gilad Almogy ,  Florian Oberstrass

    IPC: C12Q1/6869 ,  C12Q1/6876

    Abstract: The present disclosure provides methods and systems for sequencing nucleic acid molecules using a single frequency during detection, or fewer frequencies than types of nucleotide bases identified during detection. Methods and systems of the present disclosure may involve transiently binding nucleotides. Methods and systems provided herein may enable sequences to be determined at a higher accuracy and efficiency.

    METHODS FOR ACCURATE BASE CALLING USING MOLECULAR BARCODES
    67.
    发明申请
    METHODS FOR ACCURATE BASE CALLING USING MOLECULAR BARCODES 有权

    公开(公告)号：US20220162590A1

    公开(公告)日：2022-05-26

    申请号：US17546978

    申请日：2021-12-09

    Applicant: Ultima Genomics, Inc.

    Inventor： Gilad ALMOGY ,  Eyal NEISTEIN ,  Mark PRATT

    IPC: C12N15/10 ,  G16B20/20 ,  G16B40/20 ,  C12Q1/6869

    Abstract: The present disclosure provides methods for accurate base calling of sequences using molecular barcodes. A method for sequencing nucleic acid molecules may comprise: (a) using barcode molecules to barcode nucleic acid molecules from a sample, to generate barcoded nucleic acid molecules comprising barcode sequences; (b) sequencing the barcoded nucleic acid molecules to generate sequencing signals comprising signals corresponding to the barcode sequences, wherein the sequencing signals are not sequencing reads; (c) using the signals corresponding to the barcode sequences to group the sequencing signals into groups, wherein sequencing signals of a given group comprise signals corresponding to a barcode sequence that is (i) identical for the given group and (ii) different from barcode sequences of other groups; (d) processing the sequencing signals within the given group to generate sets of aggregated signals which are not sequencing reads; and (e) combining the sets of aggregated signals to generate a consensus sequence.

    RNA sequencing methods
    69.
    发明授权
    RNA sequencing methods 有权

    公开(公告)号：US11220709B2

    公开(公告)日：2022-01-11

    申请号：US17158953

    申请日：2021-01-26

    Applicant: Ultima Genomics, Inc.

    Inventor： Florian Oberstrass ,  Gilad Almogy

    IPC: C12Q1/6874 ,  C12Q1/6869 ,  C12N15/10

    Abstract: Described herein are methods for determining a sequence of a region of interest from an mRNA molecule. Sequenced polynucleotides can include a barcode region, a homopolymer region (e.g., a poly-A region), and a target region associated with the mRNA molecule. According to some methods, the barcode region omits the same base present in the homopolymer region. According to some methods, extension of the primer used for sequencing is stalled within the homopolymer region. According to some methods, sequencing flow cycles and the different barcode regions of the polynucleotides configured are such that the primer is extended to the end of the barcode region across the plurality of polynucleotides before being extended into the homopolymer region. According to some methods, two primers or a cleavable primer is used to separately sequence the barcode region and the target region.

    IMPLEMENTING BARRIERS FOR CONTROLLED ENVIRONMENTS DURING SAMPLE PROCESSING AND DETECTION
    70.
    发明申请
    IMPLEMENTING BARRIERS FOR CONTROLLED ENVIRONMENTS DURING SAMPLE PROCESSING AND DETECTION 有权

    公开(公告)号：US20210354126A1

    公开(公告)日：2021-11-18

    申请号：US17336377

    申请日：2021-06-02

    Applicant: Ultima Genomics, Inc.

    Inventor： Nathan BECKETT ,  Nathan CASWELL

    IPC: B01L3/00 ,  G01N7/02

    Abstract: Provided herein are methods for processing and/or detecting a sample. A method can comprise providing a barrier between a first region and a second region, wherein the first region comprises the sample, wherein the barrier maintains the first region at a first atmosphere that is different than a second atmosphere of the second region, wherein a portion of the barrier comprises a fluid in coherent motion; and using a detector at least partially contained in the first region to detect one or more signals from the sample while the first region is maintained at the first atmosphere that is different than the second atmosphere of the second region. The portion of the barrier comprising fluid may have a pressure lower than the first atmosphere, the second atmosphere, or both.

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