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    CHRONIC PATHOGEN-EXPRESSING CELL LINES
    61.
    发明申请
    CHRONIC PATHOGEN-EXPRESSING CELL LINES 审中-公开
    慢性病毒表达细胞系

    公开(公告)号：US20100178302A1

    公开(公告)日：2010-07-15

    申请号：US12705032

    申请日：2010-02-12

    申请人： Joseph D. Mosca

    发明人： Joseph D. Mosca

    IPC分类号： A61K39/21 ,  A61P31/18 ,  A61K31/7088 ,  C12N5/0783

    CPC分类号： C07K14/005 ,  A61K48/00 ,  A61K2039/5258 ,  C12N7/00 ,  C12N2740/16023 ,  C12N2740/16052 ,  C12N2740/16062 ,  C12N2740/16222

    摘要： This application provides a method to establish and construct cell lines expressing pathogens without destruction of the host cells. The invention allows for the formation of cell lines for the purpose of continuous expression, release, and harvesting of the pathogen and maintaining the consistency of the final biological product. Although the invention is intended for pathogen antigen expression, the invention allows for the production of any antigen by the described methods. The establishment of a chronically infected cell line can be used for reagent, diagnostic, quantification, or vaccine purposes. We have used the procedure to select for a host cell line that naturally adapts to HIV-1 replication without affecting the host cell's ability to survive. This allowed for the establishment of a chronic HIV-1 expressing cell line that continuously expresses HIV-1 particles.

    摘要翻译： 该应用提供了建立和构建表达病原体的细胞系而不破坏宿主细胞的方法。 本发明允许形成细胞系以便连续表达，释放和收获病原体并保持最终生物制品的一致性。 尽管本发明旨在用于病原体抗原表达，但是本发明允许通过所述方法产生任何抗原。 长期感染的细胞系的建立可用于试剂，诊断，定量或疫苗目的。 我们已经使用该过程来选择自然适应HIV-1复制的宿主细胞系，而不影响宿主细胞的生存能力。 这允许建立连续表达HIV-1颗粒的慢性HIV-1表达细胞系。

    MEDICAL TREATMENT DEVICE FOR TREATING AIDS BY UTILIZING MODIFIED HUMAN IMMUNODEFICIENCY VIRUS VIRIONS TO INSERT ANTI-VIRAL MEDICATIONS INTO T-HELPER CELLS
    63.
    发明申请
    MEDICAL TREATMENT DEVICE FOR TREATING AIDS BY UTILIZING MODIFIED HUMAN IMMUNODEFICIENCY VIRUS VIRIONS TO INSERT ANTI-VIRAL MEDICATIONS INTO T-HELPER CELLS 审中-公开
    用于治疗艾滋病的医疗治疗装置，其使用改良的人类免疫缺陷病毒病毒将抗病毒药物插入到T型辅助细胞

    公开(公告)号：US20090257983A1

    公开(公告)日：2009-10-15

    申请号：US12101958

    申请日：2008-04-11

    申请人： Lane Bernard Scheiber ,  Lane Bernard Scheiber, II

    发明人： Lane Bernard Scheiber ,  Lane Bernard Scheiber, II

    IPC分类号： A61K31/7088 ,  A61P31/12 ,  A61K48/00

    CPC分类号： C12N15/86 ,  A61K31/7088 ,  A61K39/00 ,  C12N2740/16023 ,  C12N2740/16042 ,  C12N2740/16045

    摘要： The medical device by which a modified Human Immunodeficiency Virus or virus-like structure is used as a transport medium to carry a payload of a quantity of anti-viral drug molecules to T-Helper cells in the body. The modified Human Immunodeficiency Virus or virus-like structure makes contact with a T-Helper cell by means of the modified virus's exterior probes or virus-like structure's exterior probes. Once the exterior probes engage the T-Helper cell's receptors, the modified virus or virus-like structure inserts into the T-Helper cell the quantity of medically therapeutic anti-viral drug molecules it is carrying. The anti-viral drug molecules exhibit an anti-viral effect when present inside the T-Helper cells thus assisting in repelling an infection by the Human Immunodeficiency Virus and the use of such a device significantly lowers the occurrence of unwanted deleterious side effects.

    摘要翻译： 使用修饰的人类免疫缺陷病毒或病毒样结构作为运输介质以将大量抗病毒药物分子的有效载荷携带到体内的T-Helper细胞的医疗装置。 修饰的人类免疫缺陷病毒或病毒样结构通过修饰的病毒的外部探针或病毒样结构的外部探针与T-Helper细胞接触。 一旦外部探针接合T-Helper细胞的受体，经修饰的病毒或病毒样结构将T-Helper细胞插入到其携带的医疗治疗性抗病毒药物分子的数量上。 当存在于T-Helper细胞内时，抗病毒药物分子表现出抗病毒作用，从而有助于排斥人类免疫缺陷病毒的感染，并且使用这种装置可显着降低不想要的有害副作用的发生。

    Non-infectious, non-replicating, immunogenic human immunodeficiency virus-like particles
    67.
    发明授权
    Non-infectious, non-replicating, immunogenic human immunodeficiency virus-like particles 失效
    非感染性，非复制型，免疫原性人类免疫缺陷病毒样颗粒

    公开(公告)号：US07008784B1

    公开(公告)日：2006-03-07

    申请号：US09389364

    申请日：1999-09-03

    申请人： Joel Haynes ,  Michel Henri Klein ,  Benjamin Rovinski ,  Shi Xian Cao

    发明人： Joel Haynes ,  Michel Henri Klein ,  Benjamin Rovinski ,  Shi Xian Cao

    IPC分类号： C12N7/04

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2740/16023 ,  C12N2740/16122

    摘要： The present invention is directed toward methods for the production of non-infectious, replication-deficient, immunogenic human immunodeficiency virus (HIV)-like particles. These particles are prepared from a recombinant expression vector comprising a heterologous promoter operatively connected to a DNA molecule comprising a modified HIV genome devoid of the long terminal repeat (LTR) regulatory regions but containing at least the gag and pol genes in their natural genomic arrangement. This vector is introduced into mammalian cells to produce the particles of interest. These particles should prove useful in a number of diagnostic, virologic, and immunologic applications.

    摘要翻译： 本发明涉及生产非感染性，复制缺陷型，免疫原性人类免疫缺陷病毒（HIV）样颗粒的方法。 这些颗粒由包含异源启动子的重组表达载体制备，所述异源启动子可操作地连接到DNA分子，所述DNA分子包含缺乏长末端重复（LTR）调节区但在其天然基因组排列中至少含有gag和pol基因的修饰的HIV基因组。 将该载体导入哺乳动物细胞以产生感兴趣的颗粒。 这些颗粒应证明在许多诊断，病毒学和免疫学应用中是有用的。

    Chronic pathogen-expressing cell lines
    68.
    发明申请
    Chronic pathogen-expressing cell lines 失效
    慢性病原体表达细胞系

    公开(公告)号：US20050074881A1

    公开(公告)日：2005-04-07

    申请号：US10486415

    申请日：2002-09-24

    申请人： Joseph Mosca

    发明人： Joseph Mosca

    IPC分类号： A61K48/00 ,  C07K14/16 ,  C12N5/02 ,  C12N5/08 ,  C12N5/10 ,  C12N7/04 ,  C07H21/04 ,  C12N5/06 ,  C12N15/85

    CPC分类号： C07K14/005 ,  A61K48/00 ,  A61K2039/5258 ,  C12N7/00 ,  C12N2740/16023 ,  C12N2740/16052 ,  C12N2740/16062 ,  C12N2740/16222

    摘要： This application provides a method to establish and construct cell lines expressing pathogens without destruction of the host cells. The invention allows for the formation of cell lines for the purpose of continuous expression, release, and harvesting of the pathogen and maintain the consistency of the final biological pro duct Although the invention is intended for pathogen antigen expression, the invention allows for the production of any antigen by the described methods. The establishment of a chronically infected celline can be used for reagent, diagnostic, quantification, or vaccine purposes. We have used the procedure to select for a host cell line that naturally adapts to HIV-1 replication without affecting the host cell's ability to survive. This allowed for the establishmentof a chronic HIV-1 expressing cell line that continuously expresses HIV-1 particles.

    摘要翻译： 该应用提供了建立和构建表达病原体的细胞系而不破坏宿主细胞的方法。 本发明允许形成用于连续表达，释放和收获病原体并维持最终生物制剂的一致性的细胞系。尽管本发明旨在用于病原体抗原表达，但本发明允许生产 任何抗原通过所述方法。 长期感染的细胞因子的建立可用于试剂，诊断，定量或疫苗目的。 我们已经使用该过程来选择自然适应HIV-1复制的宿主细胞系，而不影响宿主细胞的生存能力。 这允许建立连续表达HIV-1颗粒的慢性HIV-1表达细胞系。

    Fusion protein delivery system and uses thereof
    69.
    发明申请
    Fusion protein delivery system and uses thereof 有权
    融合蛋白递送系统及其用途

    公开(公告)号：US20050014260A1

    公开(公告)日：2005-01-20

    申请号：US10245475

    申请日：2002-09-17

    申请人： John Kappes ,  Xiaoyun Wu

    发明人： John Kappes ,  Xiaoyun Wu

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C07K14/15 ,  C12N5/10 ,  C12N15/62 ,  C12N15/63 ,  C12N15/867 ,  C12P21/00 ,  C12N15/86 ,  C12N7/00

    CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/40 ,  C07K2319/735 ,  C12N15/62 ,  C12N15/86 ,  C12N2710/16122 ,  C12N2740/10022 ,  C12N2740/16023 ,  C12N2740/16043 ,  C12N2740/16052

    摘要： The present invention provides a composition of matter, comprising: DNA encoding a viral Vpx protein fused to DNA encoding a protein. In another embodiment of the present invention, there is provided a composition of matter, comprising: DNA encoding a viral Vpr protein fused to DNA encoding a protein. The present invention further provides DNA, vectors and methods for expressing a lentiviral pol gene in trans, independent of the lentiviral gag-pol. A gene transduction element is optionally delivered to a lentiviral vector according to the present invention. Also provided are various methods of delivering a virus inhibitory molecule to a target in an animal. Further provided is a pharmaceutical composition.

    摘要翻译： 本发明提供一种物质组合物，其包含：编码与编码蛋白质的DNA融合的病毒Vpx蛋白质的DNA。 在本发明的另一个实施方案中，提供了一种物质组合物，其包含：编码与编码蛋白质的DNA融合的病毒Vpr蛋白的DNA。 本发明还提供了与慢病毒gag-pol无关的用于以反式表达慢病毒pol基因的DNA，载体和方法。 任选将基因转导元件递送至根据本发明的慢病毒载体。 还提供了将病毒抑制分子递送到动物中的靶的各种方法。 进一步提供药物组合物。