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    Method for analyzing a target nucleic acid fragment and a kit for analyzing a target nucleic acid fragment
    63.
    发明申请
    Method for analyzing a target nucleic acid fragment and a kit for analyzing a target nucleic acid fragment 审中-公开
    用于分析靶核酸片段的方法和用于分析靶核酸片段的试剂盒

    公开(公告)号：US20050266450A1

    公开(公告)日：2005-12-01

    申请号：US11106612

    申请日：2005-04-15

    申请人： Yoshihiko Makino ,  Toshihiro Mori ,  Yoshihide Iwaki

    发明人： Yoshihiko Makino ,  Toshihiro Mori ,  Yoshihide Iwaki

    IPC分类号： C12P19/34 ,  C12Q1/42 ,  C12Q1/68 ,  G01N33/52

    CPC分类号： G01N33/523 ,  C12Q1/42 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2565/625 ,  C12Q2565/301 ,  C12Q2521/543 ,  C12Q2533/101

    摘要： An object of the present invention is to provide a method for analyzing a target nucleic acid fragment which can be simply and swiftly carried out by using a small apparatus, a kit for analyzing a target nucleic acid fragment using the method for analysis, and a dry analytical element for quantifying pyrophosphoric acid. The present invention provides a method for analyzing pyrophosphoric acid generated upon polymerase elongation reaction based on certain nucleotide sequence of a target nucleic acid, a kit for analysis for carrying out the above mentioned method for analysis, and a dry analytical element for quantifying pyrophosphoric acid.

    摘要翻译： 本发明的目的是提供一种用于分析目标核酸片段的方法，其可以通过使用小型装置简单快速地进行，使用该分析方法分析靶核酸片段的试剂盒和干燥的 用于定量焦磷酸的分析元素。 本发明提供一种基于靶核酸的某些核苷酸序列分析聚合酶延伸反应产生的焦磷酸的方法，用于进行上述分析方法的分析用试剂盒和定量焦磷酸的干燥分析元素。

    Nucleic hybridization assay method and device using a cleavage technique responsive to the complementary double strand or the single strand of nucleic acids or oligonucleotides
    66.
    发明申请
    Nucleic hybridization assay method and device using a cleavage technique responsive to the complementary double strand or the single strand of nucleic acids or oligonucleotides 审中-公开
    使用对互补双链或单链核酸或寡核苷酸有反应的切割技术的核杂交测定方法和装置

    公开(公告)号：US20040234970A1

    公开(公告)日：2004-11-25

    申请号：US10470487

    申请日：2004-02-17

    发明人： Jae Chern Yoo

    IPC分类号： C12Q001/68 ,  C07H021/04

    CPC分类号： B82Y30/00 ,  C12Q1/6823 ,  Y02A90/22 ,  Y02A90/26 ,  C12Q2533/101 ,  C12Q2525/131 ,  C12Q2521/301 ,  C12Q2565/625

    摘要： A cleavable signal element applicable to quantitative and qualitative assay devices, using a cleavable technique specifically responsive to a complementary double strand or single strand of nucleic acids, and a nucleic acid hybridization assay method and device using the cleavable signal element are provided. Using the cleavable technique responsive to the complementary double strand or single strand of nucleic acids, detection sensitivity to a target nucleic acid can be increased, and diagnosis and detection reliability can be improved twice through in-situ determinations. Through simultaneous single nucleotide polymorphism (SNP) detection and expression profile determination, more accurate diagnosis for many diseases can be achieved. The assay device can be easily modified to be suitable for detection with general laser-based detection systems such as CD-ROM readers. Information read from the assay device is digitized as software and transmitted to and received by doctors and patients through a computer network or wirelessly, which enables construction of remote diagnosis systems.

    摘要翻译： 提供了可应用于定量和定性测定装置的可裂解信号元件，其使用对互补双链或单链核酸特异性响应的可切割技术，以及使用可切割信号元件的核酸杂交测定方法和装置。 使用对互补双链或单链核酸作出反应的可切割技术，可以提高对目标核酸的检测灵敏度，并通过原位测定提高诊断和检测可靠性两倍。 通过同时单核苷酸多态性（SNP）检测和表达谱确定，可以更准确地诊断许多疾病。 测定装置可以容易地被修改为适合于通常的基于激光的检测系统如CD-ROM读取器的检测。 从测定装置读取的信息被数字化为软件，并通过计算机网络或无线传输给医生和患者并由医生和患者接收，这使得能够构建远程诊断系统。

    Binding interactions in dipstick assays
    69.
    发明申请
    Binding interactions in dipstick assays 有权
    试纸测定中的结合相互作用

    公开(公告)号：US20040072176A1

    公开(公告)日：2004-04-15

    申请号：US10332142

    申请日：2003-04-07

    发明人： Helen Lee ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

    IPC分类号： C12Q001/68 ,  C12M001/34

    CPC分类号： C12Q1/689 ,  C12Q1/6832 ,  C12Q1/6834 ,  C12Q2565/519 ,  C12Q2525/197 ,  C12Q2565/625 ,  C12Q2537/125

    摘要： Use of dipsticks to test for the presence of target nucleic acid in a sample solution is described. The dipsticks comprise a contact end for contacting the sample solution and a capture zone, remote from the contact end, to which a capture probe is immobilised. The capture probe is capable of hybridising to the target nucleic acid. The sample solution is contacted with the contact end of the dipstick and travels by capillary action to the capture zone. If target nucleic acid is present in the sample solution it is captured and can be detected at the capture zone. The capture probe is immobilised to the capture zone by a spacer. Use of the spacer increases the stability of the interaction between the capture probe and the target nucleic acid and thus improves the sensitivity of target nucleic acid detection. Detection probes with spacers are also described.

    摘要翻译： 描述了使用骰子来测试样品溶液中靶核酸的存在。 骰子包括用于接触样品溶液的接触端和远离接触端的捕获区，捕获探针被固定到捕获区。 捕获探针能够与靶核酸杂交。 样品溶液与量油尺的接触端接触，并通过毛细作用行进到捕获区。 如果靶核酸存在于样品溶液中，则其被捕获并且可以在捕获区域被检测。 捕获探针通过间隔物固定在捕获区上。 间隔物的使用增加了捕获探针和目标核酸之间相互作用的稳定性，从而提高了靶核酸检测的灵敏度。 还描述了具有间隔物的检测探针。