公开(公告)号：US5702901A

公开(公告)日：1997-12-30

申请号：US495739

申请日：1995-06-08

Applicant: Thomas J. Cummins ,  Susan Melissa Atwood ,  Lynn Bergmeyer ,  John Bruce Findlay ,  John W.H. Sutherland ,  JoAnne H. Kerschner

Inventor： Thomas J. Cummins ,  Susan Melissa Atwood ,  Lynn Bergmeyer ,  John Bruce Findlay ,  John W.H. Sutherland ,  JoAnne H. Kerschner

IPC: C12Q1/68 ,  C12Q1/70 ,  C12P19/34

CPC classification number: C12Q1/708 ,  C12Q1/689 ,  C12Q1/703 ,  C12Q1/705 ,  C12Q2600/16 ,  Y10S977/728 ,  Y10S977/795 ,  Y10S977/836 ,  Y10S977/924 ,  Y10S977/958

Abstract: An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T.sub.m within the range of from about 65.degree. to about 74.degree. C., while the T.sub.m 's are within about 5.degree. C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes. All of the capture probes have T.sub.m 's which are greater than 50.degree. C. and are within 15.degree. C. of each other.

Abstract translation: 含有两个或多个靶核酸相对链的引物的水性组合物可用于聚合酶链式反应，以提供对这些核酸的同时快速和有效的扩增和检测。 每个目标DNA的引物长度不同于5个核苷酸，Tm在约65-74℃范围内，而Tm在约5℃以内。 这样的组合物可用于诊断测试试剂盒和用于多重核酸的扩增和检测的方法，或使用多种捕获探针进行“复用”。 所有的捕获探针具有大于50℃的Tm，并且彼此在15℃以内。

公开(公告)号：US5363697A

公开(公告)日：1994-11-15

申请号：US875694

申请日：1992-04-29

Applicant: Tohru Nakagawa

Inventor： Tohru Nakagawa

IPC: C12M1/34 ,  C12M1/40 ,  C12Q1/68 ,  G01B7/34 ,  G01B21/30 ,  G01N27/416 ,  G01Q60/10 ,  G01Q60/24 ,  G01Q60/38 ,  G01Q60/42 ,  G01Q60/44 ,  G01Q70/00 ,  G01B5/28

CPC classification number: G01Q60/60 ,  B82Y35/00 ,  G01Q60/42 ,  B82Y30/00 ,  Y10S977/728 ,  Y10S977/849 ,  Y10S977/852 ,  Y10S977/853 ,  Y10S977/86 ,  Y10S977/877 ,  Y10S977/879 ,  Y10S977/881 ,  Y10S977/924 ,  Y10S977/958

Abstract: This invention relates to a scanning probe microscope which examines or processes directly the structure of substance surfaces at the molecular or atomic level, and a method for processing molecules using a scanning probe microscope and a method for detecting DNA base arrangement. A scanning probe microscope has a probe approaching or contacting the substance surface to detect a physical quantity between the substance surface and the probe, wherein the physical quantity is what is interacted or chemically reacted between the substance surface and the molecules or atom groups which act as a sensor and are fixed on the probe, scanning at an atomic level of precision. Therefore, surface structure within a microscopic region can be examined or processed at the molecular or atomic level. A method for detecting DNA base arrangement with any one of three or four kinds of probes fixed with any one of four different kinds of molecules interacting four kinds of bases consisting of DNA, by approaching single stranded DNA fixed on a substrate, measuring the force and scanning by an atomic force microscope at an atomic level of precision.

Abstract translation: 本发明涉及一种扫描探针显微镜，该扫描探针显微镜直接检查分子或原子水平的物质表面的结构，以及使用扫描探针显微镜处理分子的方法和检测DNA碱基排列的方法。 扫描探针显微镜具有接近或接触物质表面的探针以检测物质表面和探针之间的物理量，其中物理量是物质表面与作为的物质表面和分子或原子团之间的相互作用或化学反应的物质 传感器并固定在探头上，以原子级精度扫描。 因此，微观区域内的表面结构可以在分子或原子水平进行检查或处理。 用四种不同种类的分子中的任何一种分离的任何一种探针中的任何一种探测DNA的检测方法，其通过接近固定在基底上的单链DNA，测量力和与 用原子力显微镜以原子精度扫描。