公开(公告)号：US11609233B2

公开(公告)日：2023-03-21

申请号：US17349710

申请日：2021-06-16

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G01N33/536 ,  G01N33/58 ,  G01N33/543 ,  G01N33/50 ,  G01N21/77 ,  G01N33/53

Abstract: An indicator of a first type and an indicator of a second type are attached to a unit of a chemical component in a sample to form a first multi-indicator complex. The first multi-indicator complex includes the unit of the chemical component, the indicator of the first type, and the indicator of the second type. The indicator of the first type and the indicator of the second type have different discernible characteristics. An image of the sample, including the first multi-indicator complex corresponding to the unit of the chemical component, is captured by an image sensor. Based on a first image of the sample, a count is generated of multi-indicator complexes that include an indicator of the first type and an indicator of the second type, including the first multi-indicator complex. Based on the count, a presence or a level of the chemical component in the sample is identified.

公开(公告)号：US20210278647A1

公开(公告)日：2021-09-09

申请号：US17193680

申请日：2021-03-05

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine ,  Joel Ironstone ,  Christopher Gillespie

IPC: G02B21/00 ,  G02B21/06

Abstract: A base assembly includes an imaging sensor having a sensor surface to receive a sample, and a platform connected to the base assembly. The base assembly includes (a) an aperture configured to receive a lid surface of a lid in a position to define an imaging space between the sensor surface and the lid surface and (b) a movement portion movable toward and away from the base assembly. The platform and the base assembly are configured to limit contact between the sample and the base assembly other than at the sensor surface.

公开(公告)号：US11099181B2

公开(公告)日：2021-08-24

申请号：US16868270

申请日：2020-05-06

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G01N33/543 ,  G01N33/50 ,  G01N21/77 ,  G01N33/53

Abstract: Among other things, two or more different antibodies are caused to bind to one or more units of a chemical component in a sample. Each of the antibodies is attached to one or more beads (e.g., microbeads). The sample is situated on a surface of an image sensor. At the image sensor, light is received originating at a light source that is other than the beads. The received light includes light reflected by, refracted by, or transmitted through the beads. At least one image of the sample is processed to separately enumerate individual beads and complexes of two or more of the beads attached to the two or more antibodies that are bound to a unit of the chemical component. The results of the processing are used to identify a presence or a level of the chemical component in the sample.

公开(公告)号：US20210116475A1

公开(公告)日：2021-04-22

申请号：US17103246

申请日：2020-11-24

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G01Q60/20 ,  G01N21/64 ,  G02B21/00 ,  G02B21/36 ,  H01L27/146

Abstract: Among other things, an imaging device has a photosensitive array of pixels, and a surface associated with the array is configured to receive a specimen with at least a part of the specimen at a distance from the surface equivalent to less than about half of an average width of the pixels.

公开(公告)号：US20210066377A1

公开(公告)日：2021-03-04

申请号：US17070743

申请日：2020-10-14

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine ,  Madhuranga Srinath Rankaduwa

IPC: H01L27/146 ,  G01N21/27 ,  G01N33/49 ,  G02B21/00

Abstract: Among other things, an integral image sensor includes a sensor surface having a surface area at which light-sensitive pixels are arranged in rows and columns. The surface area includes two or more light-sensitive subareas each of the subareas having been configured to have been diced from a wafer along two orthogonal dimensions to form a discrete image sensor. The two or more light-sensitive subareas are arranged along one of the two orthogonal dimensions. The sensor surface of the integral image sensor is flat and continuous across the two or more subareas.

公开(公告)号：US20200309772A1

公开(公告)日：2020-10-01

申请号：US16868270

申请日：2020-05-06

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G01N33/543 ,  G01N33/50 ,  G01N21/77 ,  G01N33/53

Abstract: Among other things, two or more different antibodies are caused to bind to one or more units of a chemical component in a sample. Each of the antibodies is attached to one or more beads (e.g., microbeads). The sample is situated on a surface of an image sensor. At the image sensor, light is received originating at a light source that is other than the beads. The received light includes light reflected by, refracted by, or transmitted through the beads. At least one image of the sample is processed to separately enumerate individual beads and complexes of two or more of the beads attached to the two or more antibodies that are bound to a unit of the chemical component. The results of the processing are used to identify a presence or a level of the chemical component in the sample.

公开(公告)号：US10768078B2

公开(公告)日：2020-09-08

申请号：US16367791

申请日：2019-03-28

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine ,  Hershel Macaulay

IPC: G01N1/28 ,  G01N33/49 ,  G02B21/36 ,  G02B21/00 ,  G01N1/38 ,  G01N21/31

Abstract: Among other things, a diluted sample is generated based on mixing a small sample of blood with a one or more diluents. A thin film of the diluted sample is formed on the surface of a contact optical microscopy sensor. Red blood cells within a portion of the thin film of the diluted sample are illuminated using light of a predetermined wavelength. One or more images of the diluted sample are acquired based on illuminating the red blood cells within the portion of the thin film of the diluted sample. The acquired one or more images of the diluted sample are then processed. The mean corpuscular hemoglobin in the red blood cells within the portion of the thin film of the diluted sample is determined based on processing the acquired images of the diluted sample.

公开(公告)号：US20200278529A1

公开(公告)日：2020-09-03

申请号：US16804957

申请日：2020-02-28

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G02B21/36 ,  B01L3/00 ,  G01N15/06 ,  G02B21/34 ,  G02B21/00 ,  G01N1/28 ,  G01N1/38 ,  G01N21/31 ,  G01N33/49

Abstract: In some instances, an apparatus can include a light sensitive imaging sensor having a surface to receive a fluid sample, a body to be moved relative to the light sensitive imaging sensor and having a surface to touch a portion of the fluid sample, and a carrier to move the body toward the surface of the light sensitive imaging sensor to cause the surface of the body to touch the portion of the fluid sample, so that as the surface of the body touches the portion of the fluid, the surface of the body (i) is parallel to the surface of the light sensitive imaging sensor, and (ii) settles on top of the fluid sample independently of motion of the carrier.

公开(公告)号：US10746979B2

公开(公告)日：2020-08-18

申请号：US16455482

申请日：2019-06-27

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine ,  Hershel Macaulay ,  Noah Hymes-Vandermeulen

IPC: G02B21/34 ,  G01N21/59 ,  G01N1/40 ,  G01N15/06 ,  G01N1/28 ,  G01N15/14 ,  G01N33/49 ,  G01N35/00 ,  G01N15/00 ,  G01N15/10

Abstract: Among other things, a first surface is configured to receive a sample and is to be used in a microscopy device. There is a second surface to be moved into a predefined position relative to the first surface to form a sample space that is between the first surface and the second surface and contains at least part of the sample. There is a mechanism configured to move the second surface from an initial position into the predefined position to form the sample space. When the sample is in place on the first surface, the motion of the second surface includes a trajectory that is not solely a linear motion of the second surface towards the first surface.

公开(公告)号：US10649187B2

公开(公告)日：2020-05-12

申请号：US16573565

申请日：2019-09-17

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G01N15/10 ,  G01N15/14 ,  G01N33/80 ,  G02B21/00 ,  G02B21/36 ,  G01T1/02 ,  G06T7/00 ,  G06K9/00 ,  G01N11/00 ,  G02B9/02 ,  G03H1/00 ,  G03H1/04 ,  G01B9/02 ,  G01N15/00 ,  C12M1/34

Abstract: Among other things, a method comprises imaging a sample displaced between a sensor surface and a surface of a microscopy sample chamber to produce an image of at least a part of the sample. The image is produced using lensless optical microscopy, and the sample contains at least blood from a subject. The method also comprises automatically differentiating cells of different types in the image, generating a count of one or more cell types based on the automatic differentiation, and deriving a radiation dose the subject has absorbed based on the count.