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105 条记录 (耗时 0.041 秒)

    Rhodamine derivatives and the use thereof
    72.
    发明授权
    Rhodamine derivatives and the use thereof 有权
    罗丹明衍生物及其用途

    公开(公告)号：US06184379B2

    公开(公告)日：2001-02-06

    申请号：US09324265

    申请日：1999-06-02

    申请人： Hans-Peter Josel ,  Rupert Herrmann ,  Dieter Heindl ,  Klaus Muhlegger ,  Gregor Sagner ,  Karl Heinz Drexhage ,  Jorg Frantzeskos ,  Jutta Arden-Jacob

    发明人： Hans-Peter Josel ,  Rupert Herrmann ,  Dieter Heindl ,  Klaus Muhlegger ,  Gregor Sagner ,  Karl Heinz Drexhage ,  Jorg Frantzeskos ,  Jutta Arden-Jacob

    IPC分类号： C07D49147

    CPC分类号： C09B11/24 ,  Y10S436/80

    摘要： The invention concerns rhodamine derivatives of the general formulae in which Ca-Cd each denote a C atom, and Ca and Cb as well as Cc and Cd are either linked together by a single bond or by a double bond; X1 to X16 denote independently of one another halogen, sulfonic acid, hydrogen or an alkyl residue with 1-20 C atoms in which the alkyl residue can be substituted with one or several halogen or sulfonic acid residues; R1 and R2 are either identical or different and denote either hydrogen, alkyl with 1-20 C atoms, polyoxyhydrocarbyl units, phenyl or phenylalkyl with 1-3 carbon atoms in the alkyl chain in which the alkyl and/or phenyl residues can be substituted by one or several hydroxy, halogen, sulfonic acid, amino, carboxy or alkoxycarbonyl groups where alkoxy can have 1-4 carbon atoms, R1 contains at least one activatable group, R2 and X4 can be optionally linked together via a bridge composed of 0-2 C atoms. In contrast to the prior art, these compounds are characterized in that A1, A2 and A3 can independently of one another denote hydrogen, cyano, halogen and sulfonic acid; B1 denotes either halogen, cyano or hydrogen; B2 denotes hydrogen, amide, halogen and an alkyl residue with 1-20 C atoms. In addition the invention concerns activated rhodamine derivatives, correspondingly conjugated biomolecules and their use in diagnostic systems.

    摘要翻译： 本发明涉及一般式的罗丹明衍生物，其中Ca-Cd各自表示C原子，Ca和Cb以及C c和Cd通过单键或双键连接在一起; X1至X16彼此独立地表示卤素，磺酸，氢或具有1-20个C原子的烷基残基，其中烷基残基可被一个或几个卤素或磺酸残基取代; R 1和R 2可以相同或不同，表示氢，具有1-20个C原子的烷基，聚氧烃基单元，烷基链中具有1-3个碳原子的苯基或苯基烷基，其中烷基和/或苯基残基可被 一个或几个羟基，卤素，磺酸，氨基，羧基或烷氧基羰基，其中烷氧基可以具有1-4个碳原子，R 1包含至少一个可活化基团，R 2和X 4可以任选地通过由0-2 C原子。 与现有技术相反，这些化合物的特征在于，A1，A2和A3可以彼此独立地表示氢，氰基，卤素和磺酸; B1表示卤素，氰基或氢; B2表示氢，酰胺，卤素和具有1-20个C原子的烷基。 此外，本发明涉及活化的罗丹明衍生物，相应的共轭生物分子及其在诊断系统中的用途。

    Miniaturized, high-throughput nucleic acid analysis
    75.
    发明授权
    Miniaturized, high-throughput nucleic acid analysis 有权
    小型化，高通量核酸分析

    公开(公告)号：US08822158B2

    公开(公告)日：2014-09-02

    申请号：US13746812

    申请日：2013-01-22

    申请人： Thomas Froehlich ,  Dieter Heindl ,  Angelika Roesler ,  Tobias Heckel

    发明人： Thomas Froehlich ,  Dieter Heindl ,  Angelika Roesler ,  Tobias Heckel

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6874 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q2563/149 ,  C12Q2525/197 ,  C12Q2523/319 ,  C12Q2565/537 ,  C12Q2547/101 ,  C12Q2537/143

    摘要： The present invention is directed to method for analyzing multiple nucleic acid molecules of interest comprising in the steps of (i) providing a plurality of beads, characterized in that each bead comprises at least two sequence specific amplification primers, further characterized in that at least one of the primers is bound to the bead via a cleavable linker, (ii) capturing the nucleic acid molecules of interest from a sample, (iii) clonally isolating the plurality of beads, (iv) cleaving the at least one primer, (v) clonally amplifying the nucleic acid thereby creating multiple amplification products, and (vi) analyzing the amplification products.

    摘要翻译： 本发明涉及用于分析感兴趣的多个核酸分子的方法，包括以下步骤：（i）提供多个珠粒，其特征在于每个珠粒包含至少两个序列特异性扩增引物，其特征还在于至少一个 的引物通过可切割的接头与珠结合，（ii）从样品中捕获感兴趣的核酸分子，（iii）克隆分离多个珠，（iv）切割至少一个引物，（v） 克隆扩增核酸从而产生多个扩增产物，和（vi）分析扩增产物。

    Directed synthesis of oligophosphoramidate stereoisomers
    78.
    发明授权
    Directed synthesis of oligophosphoramidate stereoisomers 失效
    导向合成寡聚氨基磷酸酯立体异构体

    公开(公告)号：US08569515B2

    公开(公告)日：2013-10-29

    申请号：US13099630

    申请日：2011-05-03

    申请人： Dieter Heindl ,  Dirk Kessler ,  Angelika Roesler ,  Christoph Seidel ,  Wilma Thuer

    发明人： Dieter Heindl ,  Dirk Kessler ,  Angelika Roesler ,  Christoph Seidel ,  Wilma Thuer

    IPC分类号： C07F9/24

    CPC分类号： G01N33/5308 ,  C07F9/6552 ,  C07F9/65586 ,  Y02P20/55

    摘要： The trivalent phosphorous atom of a compound is reacted with a reagent in such a manner that a stable phosphate mimetic or a specifier is formed. Phosphoramidites with a phosphorous atom containing at least one hydroxyl residue which is provided with a protective group are reacted for this purpose with a free hydroxyl group: In the first synthesis cycle the hydroxyl group is linked to a solid support via a cleavable or non-cleavable linker. In further synthesis cycles the hydroxyl group is created by cleavage of the protective group from the growing oligomer. This results in formation of a phosphorous acid triester which is reacted with azides. By selecting suitable monomers for the synthesis which have a defined stereoconformation compounds of Formula 1 are produced in a stereocontrolled manner.

    摘要翻译： 使化合物的三价磷原子与试剂反应，形成稳定的磷酸酯模拟物或说明物。 具有至少一个含有保护基团的羟基残基的磷原子的亚磷酰胺与该游离羟基反应：在第一个合成循环中，羟基通过可裂解或不可裂解的方式与固体支持物连接 接头。 在进一步的合成循环中，通过从生长的低聚物中切割保护基而产生羟基。 这导致形成与叠氮化物反应的亚磷酸三酯。 通过选择具有定义的立体定构的合成合成单体，以立体控制的方式制备式1的化合物。

    DETECTING THE DECOMPOSITION OF ENZYMES IN A TEST ELEMENT BY MEANS OF CONTROLLED RELEASE OF A PROTECTED ANALYTE
    79.
    发明申请
    DETECTING THE DECOMPOSITION OF ENZYMES IN A TEST ELEMENT BY MEANS OF CONTROLLED RELEASE OF A PROTECTED ANALYTE 审中-公开
    通过受保护分析仪的控制释放检测测试单元中酶的分解

    公开(公告)号：US20130052674A1

    公开(公告)日：2013-02-28

    申请号：US13524199

    申请日：2012-06-15

    申请人： Carina Horn ,  Dieter Heindl ,  Hans-Peter Haar ,  Nelli Steinke

    发明人： Carina Horn ,  Dieter Heindl ,  Hans-Peter Haar ,  Nelli Steinke

    IPC分类号： C12Q1/32 ,  G01N21/77 ,  C12Q1/54

    CPC分类号： C12Q1/54

    摘要： In one form, a diagnostic element for determining at least one analyte is provided. In a further form, an analytical measuring device includes the diagnostic element. Still, other forms are related to methods for the determination of an analyte, correcting a signal generated by an analyte, and/or checking the detection optics of an analytical measuring device using the diagnostic element. Another form is related to a system for the controlled release of a reagent and the use of such a system as a circuit element. Other aspects include, but are not limited to, unique methods, techniques, products, systems and devices involving diagnostic elements.

    摘要翻译： 在一种形式中，提供了用于确定至少一种分析物的诊断元件。 在另一种形式中，分析测量装置包括诊断元件。 仍然，其它形式涉及用于确定分析物的方法，校正由分析物产生的信号和/或使用诊断元件检查分析测量装置的检测光学元件。 另一种形式涉及用于控制释放试剂的系统以及使用诸如电路元件的系统。 其他方面包括但不限于涉及诊断元件的独特方法，技术，产品，系统和设备。