公开(公告)号：US06190865B1

公开(公告)日：2001-02-20

申请号：US08534799

申请日：1995-09-27

申请人： Jerome J. Jendrisak ,  Leslie M. Hoffman ,  Robert E. Smith

发明人： Jerome J. Jendrisak ,  Leslie M. Hoffman ,  Robert E. Smith

IPC分类号： C12Q168

CPC分类号： C12Q1/6869 ,  C12Q1/6848 ,  C12Q2525/119 ,  C12Q2525/117 ,  C12Q2521/531 ,  C12Q2525/101

摘要： A method of characterizing a nucleic acid molecule is disclosed. The method comprises synthesizing DNA in the presence of a reaction mixture comprising a nucleic acid template, a primer molecule, an enzyme that extends the primer so that a DNA molecule may be synthesized, four canonical deoxynucleoside triphosphates and at least one non-canonical deoxynucleoside triphosphate. The non-canonical deoxynucleoside triphosphate is incorporated into the synthesized DNA in place of a portion of only one canonical deoxynucleoside triphosphate. The synthesized DNA is treated with an N-glycosylase that excises a base portion of the non-canonical deoxynucleoside triphosphate from the synthesized DNA. The DNA is then treated in such a manner that the phosphodiester backbone of the DNA is broken at the abasic site, thus creating at least two DNA fragments. The fragments are separated according to size.

摘要翻译： 公开了表征核酸分子的方法。 该方法包括在包含核酸模板，引物分子，延伸引物以使得可以合成DNA分子的酶的反应混合物存在下合成DNA，四种典型脱氧核苷三磷酸和至少一种非标准脱氧核苷三磷酸 。 将非标准脱氧核苷三磷酸引入合成的DNA中代替仅一个典型脱氧核苷三磷酸的一部分。 合成的DNA用从合成的DNA中切除非标准脱氧核苷三磷酸的碱基的N-糖基化酶处理。 然后以这样的方式处理DNA，使得DNA的磷酸二酯骨架在脱碱基位点断裂，从而产生至少两个DNA片段。 碎片根据大小分离。

公开(公告)号：US6147188A

公开(公告)日：2000-11-14

申请号：US17276

申请日：1998-02-02

申请人： Mary P. Zimmerman ,  Robert E. Smith ,  Mark Becker

发明人： Mary P. Zimmerman ,  Robert E. Smith ,  Mark Becker

IPC分类号： C07D239/88 ,  A61K31/34 ,  A61K31/341 ,  A61K31/35 ,  A61K31/365 ,  A61K31/37 ,  A61K31/38 ,  A61K31/381 ,  A61K31/382 ,  A61K31/40 ,  A61K31/44 ,  A61K31/4406 ,  A61K31/443 ,  A61K31/47 ,  A61K31/472 ,  A61K31/495 ,  A61K31/496 ,  A61K31/505 ,  A61K31/517 ,  A61K31/535 ,  A61K31/5355 ,  A61K31/5375 ,  A61K38/00 ,  A61K47/48 ,  A61P1/02 ,  A61P9/00 ,  A61P9/08 ,  A61P9/10 ,  A61P25/00 ,  A61P25/02 ,  A61P25/28 ,  A61P27/02 ,  A61P27/12 ,  A61P27/14 ,  A61P29/00 ,  A61P31/00 ,  A61P31/12 ,  A61P33/02 ,  A61P35/00 ,  A61P37/08 ,  A61P43/00 ,  C07D207/16 ,  C07D213/64 ,  C07D213/65 ,  C07D213/80 ,  C07D215/22 ,  C07D215/26 ,  C07D217/22 ,  C07D235/26 ,  C07D239/34 ,  C07D295/215 ,  C07D307/58 ,  C07D307/60 ,  C07D307/62 ,  C07D309/32 ,  C07D309/38 ,  C07D309/40 ,  C07D311/16 ,  C07D311/18 ,  C07D311/46 ,  C07D333/64 ,  C07D333/68 ,  C07D335/02 ,  C07D405/12 ,  C07D487/04 ,  C07K5/062 ,  C07K5/065 ,  C07K14/81 ,  C07K5/00 ,  C07K7/00

CPC分类号： C07D405/12 ,  A61K31/34 ,  A61K31/341 ,  A61K31/35 ,  A61K31/40 ,  A61K31/44 ,  A61K31/443 ,  A61K31/505 ,  A61K31/5375 ,  A61K47/48238 ,  C07D207/16 ,  C07D213/64 ,  C07D213/80 ,  C07D239/34 ,  C07D295/215 ,  C07D307/58 ,  C07D307/60 ,  C07D307/62 ,  C07D309/38

摘要： Cysteine protease inhibitors which deactivate the protease by covalently bonding to the cysteine protease and releasing the enolate of a 1,3-dicarbonyl (or its enolic form). The cysteine protease inhibitors of the present invention accordingly comprise a first portion which targets a desired cysteine protease and positions the inhibitor near the thiolate anion portion of the active site of the protease, and a second portion which covalently bonds to the cysteine protease and irreversibly deactivates that protease by providing a carbonyl or carbonyl-equivalent which is attacked by the thiolate anion of the active site of the cysteine protease to sequentially cleave a .beta.-dicarbonyl enol ether leaving group.

摘要翻译： 半胱氨酸蛋白酶抑制剂，其通过共价键合到半胱氨酸蛋白酶并释放1,3-二羰基（或其烯醇形式）的烯醇化物而使蛋白酶失活。 因此，本发明的半胱氨酸蛋白酶抑制剂包含第一部分，其靶向所需的半胱氨酸蛋白酶，并将抑制剂置于蛋白酶活性位点的硫醇酸阴离子部分附近，第二部分与半胱氨酸蛋白酶共价结合并不可逆地失活 该蛋白酶通过提供被半胱氨酸蛋白酶的活性位点的硫醇盐阴离子攻击的羰基或羰基等价物来顺序地裂解β-二羰基烯醇醚离去基团。

公开(公告)号：US5979499A

公开(公告)日：1999-11-09

申请号：US732534

申请日：1996-10-15

申请人： Robert E. Smith

发明人： Robert E. Smith

IPC分类号： F16L17/02 ,  F16L17/08 ,  F16L29/04 ,  F16L29/00

CPC分类号： F16L17/02 ,  F16L17/08 ,  F16L29/04 ,  Y10T137/87957

摘要： An undersea hydraulic coupling having a ring-shaped metal seal that is held in place between the female member body and a sleeve-shaped seal retainer is disclosed. The annular metal seal includes a lip or leg section which is clamped in place between the female member body and the seal retainer. A hollow portion of the seal extends radially inwardly from the lip portion of the seal and is expansible to form a fluid tight seal against the body of the male member. The hollow portion of the metal seal is preferably cylindrical in cross-section and is sufficiently flexible in response to fluid pressure to form a fluid tight seal between the female member, male member, and seal retainer.

摘要翻译： 公开了一种具有环形金属密封件的海底液压联轴节，该环形金属密封件保持在阴构件主体和套筒形密封件保持器之间的适当位置。 环形金属密封件包括唇部或腿部，其被夹紧在阴构件主体和密封件保持器之间的适当位置。 密封件的中空部分从密封件的唇部径向向内延伸并且可膨胀以形成抵靠阳件的主体的流体密封。 金属密封件的中空部分优选是圆柱形的横截面，并且响应于流体压力足够柔性以在阴构件，阳构件和密封件保持器之间形成流体密封。

公开(公告)号：US5665193A

公开(公告)日：1997-09-09

申请号：US452604

申请日：1995-05-25

申请人： Charles T. Groswith, III ,  Eugene F. Duval ,  Roger M. Gray ,  Barry C. Kockler ,  Robert E. Smith

发明人： Charles T. Groswith, III ,  Eugene F. Duval ,  Roger M. Gray ,  Barry C. Kockler ,  Robert E. Smith

IPC分类号： B41F19/06 ,  B41J1/00 ,  B41J1/24 ,  B41J1/30 ,  B41J3/28

CPC分类号： B41J1/00 ,  B41F19/068 ,  B41J1/24 ,  B41J1/30 ,  B41P2219/20 ,  B41P2219/434 ,  Y10T156/171

摘要： A method of debossing character and indicia-forming pigmented tape onto a report or book cover workpiece positioned under a print engine having a character finger daisy wheel forcer and a logo die or other indicia die forcer is described. Steps of inserting a print wheel and a first tape cartridge into the engine, heating a character-containing portion of a character finger, forcing and printing a desired character in a character debossment operation are included. In another operation, the steps of removing the daisy wheel and the first tape cartridge, inserting an indicia-forming debossment die and a second tape cartridge, heating the die and forcing and thermally printing desired indicia on the workpiece, is set forth. In another aspect of the method, the steps of holding the tape on reels in the cartridges, striking the tape to deboss the pigmented media, stripping a struck portion of the tape from the workpiece and then advancing a tape take-up reel are set forth. In another embodiment, the steps of determining residual heat in a character portion or die and varying the dwell time of subsequent strikings of the workpiece accordingly, are included.

摘要翻译： 描述了将字符和形成标记的着色胶带压到位于具有字符手指菊花车轮和标志模或其他标记模具的打印引擎下的报告或书封面工件上的方法。 包括将打印轮和第一带盒插入发动机中的步骤，加热字符手指的字符包含部分，强制并打印字符压缩操作中所需的字符。 在另一操作中，阐述了去除菊花轮和第一带盒的步骤，插入形成标记的凹陷模和第二带盒，加热模具并迫使和热打印工件上的期望标记。 在该方法的另一方面，将带保持在盒中的卷轴上的步骤，撞击带以使着色介质凹陷，从工件剥离带的冲击部分，然后推进卷带卷轴 。 在另一个实施例中，包括确定字符部分或管芯中的残余热量并相应地改变随后工件的停留时间的步骤。

公开(公告)号：US5663380A

公开(公告)日：1997-09-02

申请号：US599459

申请日：1996-01-22

申请人： Mary P. Zimmerman ,  Robert E. Smith

发明人： Mary P. Zimmerman ,  Robert E. Smith

IPC分类号： A61K31/34 ,  A61K31/35 ,  A61K31/365 ,  A61K31/40 ,  A61K31/44 ,  A61K31/4406 ,  A61K31/443 ,  A61K31/505 ,  A61K31/5375 ,  A61K38/00 ,  A61K47/48 ,  A61P1/02 ,  A61P9/00 ,  A61P9/08 ,  A61P9/10 ,  A61P25/02 ,  A61P25/28 ,  A61P27/02 ,  A61P27/12 ,  A61P27/14 ,  A61P29/00 ,  A61P31/12 ,  A61P33/02 ,  A61P35/00 ,  A61P37/08 ,  A61P43/00 ,  C07D207/16 ,  C07D213/64 ,  C07D213/65 ,  C07D213/80 ,  C07D239/34 ,  C07D295/215 ,  C07D307/58 ,  C07D307/60 ,  C07D307/62 ,  C07D309/32 ,  C07D309/38 ,  C07D405/12 ,  C07K14/81 ,  C07D315/00 ,  C07D307/02

CPC分类号： C07D213/64 ,  A61K31/34 ,  A61K31/35 ,  A61K31/40 ,  A61K31/44 ,  A61K31/443 ,  A61K31/505 ,  A61K31/5375 ,  A61K47/48238 ,  C07D207/16 ,  C07D213/80 ,  C07D239/34 ,  C07D295/215 ,  C07D307/58 ,  C07D307/60 ,  C07D307/62 ,  C07D309/38 ,  C07D405/12

摘要： A class of cysteine protease inhibitors which inactivate a cysteine protease by covalently bonding to the protease and releasing a heterocyclic leaving group is presented. The cysteine protease inhibitors of the present invention comprise a first portion which targets a desired cysteine protease and positions the inhibitor near the thiolate anion portion of the active site of the protease, and a second portion which covalently bonds to the cysteine protease and irreversibly deactivates that protease by providing a carbonyl or carbonyl-equivalent which is attacked by the thiolate anion of the active site of the cysteine protease to sequentially cleave a heterocyclic leaving group. The heterocyclic leaving group of the protease inhibitor is of the formula: --O--Het, where Het is a heterocycle having 4-7 atoms in the ring, with at least one of the heterocycle atoms being N, O or S.

摘要翻译： 提出了一类半胱氨酸蛋白酶抑制剂，其通过共价键合蛋白酶和释放杂环离去基团而使半胱氨酸蛋白酶失活。 本发明的半胱氨酸蛋白酶抑制剂包含第一部分，其靶向所需的半胱氨酸蛋白酶，并将抑制剂置于蛋白酶活性位点的硫醇盐阴离子部分附近，第二部分与半胱氨酸蛋白酶共价结合，并且不可逆地使 通过提供被半胱氨酸蛋白酶的活性位点的硫醇盐阴离子攻击的羰基或羰基等价物来顺序地切割杂环离去基团。 蛋白酶抑制剂的杂环离去基团具有下式：-O-Het，其中Het是在环中具有4-7个原子的杂环，其中至少一个杂环原子是N，O或S.

公开(公告)号：US5232021A

公开(公告)日：1993-08-03

申请号：US968371

申请日：1992-10-29

申请人： Robert E. Smith

发明人： Robert E. Smith

IPC分类号： E21B33/035 ,  E21B33/038 ,  F16L37/32

CPC分类号： E21B33/0355 ,  E21B33/038 ,  F16L37/32 ,  Y10S285/917 ,  Y10T137/87957

摘要： A probe member for an undersea hydraulic coupling is disclosed having at least one circumferential depression in its outer cylindrical body, for disengaging one or more radial seals during insertion or withdrawal of the probe member from the receiving member of a coupling. The disengagement of the radial seals allows seawater to bleed from or into the space between the coupling members and thereby balance the coupling and seals to seawater pressure.

摘要翻译： 公开了一种用于海底液压联轴器的探针构件，其在其外圆柱体中具有至少一个周向凹陷，用于在将探针构件从联接器的接收构件插入或取出期间分离一个或多个径向密封。 径向密封件的分离允许海水从联接构件之间的空间渗出或进入到联接构件之间的空间，从而平衡联接并将其密封到海水压力。

公开(公告)号：US4913150A

公开(公告)日：1990-04-03

申请号：US897663

申请日：1986-08-18

申请人： Peter W. Cheung ,  Karl F. Gauglitz ,  Scott W. Hunsaker ,  Stephen J. Prosser ,  Darrell O. Wagner ,  Robert E. Smith

发明人： Peter W. Cheung ,  Karl F. Gauglitz ,  Scott W. Hunsaker ,  Stephen J. Prosser ,  Darrell O. Wagner ,  Robert E. Smith

IPC分类号： G01N21/31 ,  A61B5/00 ,  A61B5/024 ,  A61B5/145 ,  A61B5/1455 ,  G01N21/27

CPC分类号： A61B5/02427 ,  A61B5/14552 ,  A61B5/1495 ,  G01N21/274 ,  A61B2560/0252 ,  A61B2562/08

摘要： Under the present invention, a method and apparatus are provided for compensating for the effect temperature variations have on the wavelength of light emitted by the oximeter sensor light sources (40, 42). In pulse oximetry, LEDs are typically employed to expose tissue to light at two different wavelengths. The light illuminating the tissue is received by a detector (38) where signals proportional to the intensity of light are produced. These signals are then processed by the oximeter circuitry to produce an indication of oxygen saturation. Because current oximetry techniques are dependent upon the wavelengths of light emitted by the LEDs (40-42), the wavelengths must be known. Even when predetermined combinations of LEDs (40-42) having relatively precise wavelengths are employed, variations in the wavelength of light emitted may result. Because the sensor (12) may be exposed to a significant range of temperatures while in use, the effect of temperature on the wavelengths may be significant. To compensate for this effect, a temperature sensor (50) is included in the sensor (12) to produce a signal indicative of sensor temperature. This signal is interpreted by the oximeter circuitry including, for example, a microcomputer (16), where the effect of temperature on wavelength is compensated for. In a preferred arrangement, this compensation takes the form of a computation of an alternative calibration curve from which the oxygen saturation is indicated, given a particular processing of signals from the detector (38).

公开(公告)号：US4819646A

公开(公告)日：1989-04-11

申请号：US897664

申请日：1986-08-18

申请人： Peter W. Cheung ,  Karl F. Gauglitz ,  Lee R. Mason ,  Stephen J. Prosser ,  Robert E. Smith ,  Darrell O. Wagner ,  Scott W. Hunsaker

发明人： Peter W. Cheung ,  Karl F. Gauglitz ,  Lee R. Mason ,  Stephen J. Prosser ,  Robert E. Smith ,  Darrell O. Wagner ,  Scott W. Hunsaker

IPC分类号： A61B5/145 ,  A61B5/00 ,  A61B5/1455 ,  A61B6/00

CPC分类号： A61B5/14551

摘要： A feedback control system is disclosed for use in processing signals employed in pulse transmittance oximetry. The signals are produced in response to light transmitted through, for example, a finger at two different wavelengths. Each signal includes a slowly varying baseline component representing the relatively fixed attenuation of light produced by bone, tissue, skin, and hair. The signals also include pulsatile components representing the attenuation produced by the changing blood volume and oxygen saturation within the finger. The signals are processed by the feedback control system before being converted by an analog-to-digital (A/D) converter (72) for subsequent analysis by a microcomputer (16). The feedback control system includes a controllable offset subtractor (66), a programmable gain amplifier (68), controllable drivers (44) for the light sources (40,42), and the microcomputer (16). The microcomputer receives signals from the offset subtractor (66), gain amplifier (68), drivers (44) and A/D converter (72) to produce signals that control the function of the subtractor (66) and drivers (44) in the following manner. Normally, the drivers (44) are maintained within a predetermined current range. In the event the microcomputer (16) senses an output from the converter (72) that is not within a predetermined range, the drive signal is adjusted to produce an acceptable signal. The magnitude of the offset removed by the subtractor (66), as controlled by the microcomputer (16), is maintained at a constant level when the converter (72) output is within a first predetermined range and is a predetermined function of the converter (72) output when that output falls within a second predetermined range.

公开(公告)号：US4800495A

公开(公告)日：1989-01-24

申请号：US897750

申请日：1986-08-18

申请人： Robert E. Smith

发明人： Robert E. Smith

IPC分类号： A61B5/145 ,  A61B5/00 ,  A61B5/02 ,  A61B5/1455 ,  G06F17/00 ,  G06F15/42

CPC分类号： A61B5/14551 ,  A61B5/7239

摘要： The present invention relates to the processing of signals containing information about the pulse rate and oxygen saturation of arterial blood flowing in tissue. These signals have a relatively periodic pulsatile component superimposed upon a varying baseline component. To determine the pulse rate and oxygen saturation from the signals, the positive peaks, negative peaks, and period of the signal must be determined. The present invention accomplishes this by first searching for a sustained positive sloping region of the signal. Then the first derivative of the signal with respect to time is analyzed and points on the signal before and after the occurrence of a slope reversal marked. If the slope at the first point is positive, the interval between the two points is searched for a maximum amplitude that is identified as a positive peak. After the occurrence of a negative sloping region of the signal, another pair of points are marked occurring before and after a subsequent slope reversal. The minimum amplitude of the signal between these points is then identified as a negative peak. For improved accuracy, these positive and negative peaks are then compared with waveform templates to determine whether the amplitude between the peaks falls within an allowable range and to determine whether the interval between the peaks likewise falls within an acceptable range. These ranges are adjustable in proportion to the amplitude and interval compared against them. In this manner, values for the positive peak, negative peak, and period of the signal can be determined with high reliability.

公开(公告)号：US4717906A

公开(公告)日：1988-01-05

申请号：US922005

申请日：1986-10-22

申请人： Robert E. Smith

发明人： Robert E. Smith

IPC分类号： G10K9/13 ,  G08B3/00 ,  G10K9/00

CPC分类号： G10K9/13

摘要： An electric horn, especially adapted for automotive vehicles includes a pole piece and adjusting cap for the driving motor. The pole piece and adjusting cap include a cylindrical shank and an enlarged head including a conical flange. The flange terminates in an annular rim which is in contact with and secured to the horn housing by resistance welding. A magnetic circuit for the motor extends from the pole face of the pole piece and adjusting cap through the shank and the head to the housing and the cross-sectional area for the flux path through the head is at least as great as that in the shank and sufficiently large to avoid undue magnetic saturation. The rim of the enlarged head has a diameter greater than that of the shank and less than the diameter of the housing coil cup to thereby obtain increased stiffness in the connection of the pole piece and adjusting cap with the housing.

摘要翻译： 特别适用于汽车的电喇叭包括用于驱动电机的极片和调节帽。 极片和调节盖包括圆柱形柄和包括圆锥形凸缘的扩大头部。 凸缘终止于通过电阻焊接与喇叭壳体接触并固定在其上的环形边缘。 用于电机的磁路从极片的极面和调节帽通过柄和头部延伸到壳体，并且用于通过头部的通路的横截面面积至少与柄中的那样大 并且足够大以避免不适当的磁饱和。 扩大头部的边缘的直径大于柄部的直径并且小于壳体线圈杯的直径，从而在极靴和调节帽与壳体的连接中获得增加的刚度。