公开(公告)号：US20060240532A1

公开(公告)日：2006-10-26

申请号：US11476572

申请日：2006-06-29

申请人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

发明人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

IPC分类号： C12P13/04 ,  C12P13/22 ,  C07H21/04 ,  C12N9/80 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N9/80 ,  C12N9/86 ,  C12P13/04 ,  C12P41/009 ,  C12Y305/01077 ,  C12Y305/02002

摘要： The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence set forth in SEQ ID NO:1 in the Sequence. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence set forth in SEQ ID NO:3 in the Sequence.

摘要翻译： 本发明提供了一种通过分离涉及将5-取代的乙内酰脲或N-氨基甲酰基氨基酸转化成5-取代的乙内酰脲或N-氨基甲酰基氨基酸的能力的乙内酰脲酶基因和N-氨基甲酰基-L-氨基酸水解酶基因从5-取代的乙内酰脲生产旋光性氨基酸的方法 具有上述能力的微杆菌属的微生物的旋光活性氨基酸，通过改善基因扩增和转录和翻译活性，从而制备重组体，其中产生的所需酶的量增加。 乙内酰脲酶基因是例如编码具有乙内酰脲酶活性的蛋白质的DNA，其具有序列中SEQ ID NO：1所示的核苷酸序列。 N-氨基甲酰基-L-氨基酸水解酶基因是例如编码具有N-氨基甲酰基-L-氨基酸水解酶活性的蛋白质的DNA，其具有SEQ ID NO：3所示的核苷酸序列 序列。

公开(公告)号：US20060205030A1

公开(公告)日：2006-09-14

申请号：US11433467

申请日：2006-05-15

申请人： Yasuhiro Takenaka ,  Ikuo Kira ,  Kenzo Yokozeki

发明人： Yasuhiro Takenaka ,  Ikuo Kira ,  Kenzo Yokozeki

IPC分类号： C12P21/06 ,  C12N9/80 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N9/86 ,  C12N9/80 ,  C12P13/04

摘要： DNA for encoding a protein having D-hydantoinase activity which has a base sequence represented by Sequence ID No. 1 in the Sequence Listing. DNA for encoding a protein having D-carbamylase activity which has a base sequence represented by Sequence ID No. 3 in the Sequence Listing.

公开(公告)号：US07098020B2

公开(公告)日：2006-08-29

申请号：US10289360

申请日：2002-11-07

申请人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

发明人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

IPC分类号： C12N9/90 ,  C12N15/61 ,  C12P13/04 ,  C12P13/22

CPC分类号： C12N9/80 ,  C12N9/86 ,  C12P13/04 ,  C12P41/009 ,  C12Y305/01077 ,  C12Y305/02002

摘要： The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence set forth in SEQ ID NO:1 in the Sequence. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence set forth in SEQ ID NO:3 in the Sequence.

公开(公告)号：US20060188976A1

公开(公告)日：2006-08-24

申请号：US11311467

申请日：2005-12-20

申请人： Rie Takeshita ,  Isao Abe ,  Masakazu Sugiyama ,  Kenzo Yokozeki ,  Seiichi Hara ,  Sonoko Suzuki ,  Shunichi Suzuki ,  Kunihiko Watanabe

发明人： Rie Takeshita ,  Isao Abe ,  Masakazu Sugiyama ,  Kenzo Yokozeki ,  Seiichi Hara ,  Sonoko Suzuki ,  Shunichi Suzuki ,  Kunihiko Watanabe

IPC分类号： C12N9/48 ,  C07H21/04 ,  C12P21/06 ,  C12N1/21 ,  C12N15/74

CPC分类号： C07K14/00 ,  C07K14/195 ,  C12N9/93

摘要： The present invention is to provide an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. A peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II): (I) The mutant protein having the amino acid sequence containing one or more mutations of the above mutations 1 to 38 in the amino acid sequence described in SEQ ID NO:2; and (II) The mutant protein having the amino acid sequence containing one or more mutations selected from the group consisting of substitution, deletion, insertion, addition and inversion at positions other than one or more mutation positions of the above mutation 1 to 38 in the mutant protein described in the above (I), and having a peptide-synthesizing activity.

摘要翻译： 本发明提供优异的肽合成蛋白质和有效生产肽的方法。 在下列（I）和（II）所示的至少一种蛋白质的存在下，使胺组分和羧基组分反应合成肽：（I）具有含有一个或多个突变的氨基酸序列的突变蛋白 在SEQ ID NO：2中描述的氨基酸序列中的上述突变1至38; 和（II）具有在上述变异株1〜38中的一个以上突变位置以外的位置上具有选自取代，缺失，插入，添加和倒位的一个或多个突变的氨基酸序列的突变蛋白质 上述（I）所述的突变型蛋白质，具有肽合成活性。

公开(公告)号：US20060183892A1

公开(公告)日：2006-08-17

申请号：US11405455

申请日：2006-04-18

申请人： Yasuhiro Takenaka ,  Ikuo Kira ,  Kenzo Yokozeki

发明人： Yasuhiro Takenaka ,  Ikuo Kira ,  Kenzo Yokozeki

IPC分类号： C12N1/00

CPC分类号： C12N9/80 ,  C12N9/86 ,  C12P41/009

摘要： DNA for encoding a protein having D-hydantoinase activity which has a base sequence represented by Sequence ID No. 1 in the Sequence Listing. DNA for encoding a protein having D-carbamylase activity which has a base sequence represented by Sequence ID No. 3 in the Sequence Listing.

公开(公告)号：US07060485B2

公开(公告)日：2006-06-13

申请号：US10189389

申请日：2002-07-08

申请人： Yasuhiro Takenaka ,  Ikuo Kira ,  Kenzo Yokozeki

发明人： Yasuhiro Takenaka ,  Ikuo Kira ,  Kenzo Yokozeki

IPC分类号： C12N9/78 ,  C12N15/55 ,  C12P13/04

CPC分类号： C12N9/80 ,  C12N9/86 ,  C12P41/009

摘要： DNA for encoding a protein having D-hydantoinase activity which has a base sequence represented by Sequence ID No. 1 in the Sequence Listing. DNA for encoding a protein having D-carbamylase activity which has a base sequence represented by Sequence ID No. 3 in the Sequence Listing.

公开(公告)号：US20050032187A1

公开(公告)日：2005-02-10

申请号：US10775178

申请日：2004-02-11

申请人： Kenzo Yokozeki ,  Sonoko Suzuki ,  Seiichi Hara ,  Satoshi Katayama

发明人： Kenzo Yokozeki ,  Sonoko Suzuki ,  Seiichi Hara ,  Satoshi Katayama

IPC分类号： C12N1/21 ,  C12N9/00 ,  C12N9/52 ,  C12P21/02 ,  C12P21/06 ,  C07H21/04

CPC分类号： C12N9/93 ,  C12N9/52 ,  C12P21/02 ,  C12R1/01

摘要： The present invention relates to a novel enzyme that allows peptide to be produced easily, inexpensively and at high yield without going through a complex synthesis method. More particularly, the present invention provides a novel enzyme that catalyzes a peptide-producing reaction from a carboxy component and an amine component, a microbe that produces the enzyme, and a method for inexpensive production of peptides using this enzyme or microbe. The novel enzyme that efficiently produces peptide was discovered from a newly discovered microbe belonging to the genus Empedobacter, and a method was found that allows peptides to be produced inexpensively and easily.

摘要翻译： 本发明涉及一种能够容易地，低成本地以高产率生产肽而不经过复合合成方法的新型酶。 更具体地说，本发明提供了一种从羧基组分和胺组分催化产生肽的反应的新型酶，产生酶的微生物，以及使用该酶或微生物廉价生产肽的方法。 从新发现的属于Empedobacter属的微生物发现有效产生肽的新型酶，并且发现能够廉价且容易地生产肽的方法。

公开(公告)号：US06777208B2

公开(公告)日：2004-08-17

申请号：US09983657

申请日：2001-10-25

申请人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

发明人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

IPC分类号： C12P1938

CPC分类号： C12P19/38 ,  C12P19/40

摘要： A 2′-deoxyribonucleoside is produced by culturing a microorganism, which is transformed with a gene encoding a ribonucleotide reductase and in which 2′-deoxyribonucleoside degradation activity is decreased or eliminated by disrupting a gene encoding a purine nucleoside phosphorylase on chromosomal DNA, in a medium in which the microorganism can grow to produce the 2′-deoxyribonucleoside.

摘要翻译： 通过培养用编码核糖核苷酸还原酶的基因转化的微生物，通过在染色体DNA上破坏编码嘌呤核苷磷酸化酶的基因而降低或消除2'-脱氧核糖核苷降解活性的2'-脱氧核糖核苷 其中微生物能够生长以产生2'-脱氧核糖核苷的培养基。

公开(公告)号：US06579699B1

公开(公告)日：2003-06-17

申请号：US09371010

申请日：1999-08-10

申请人： Yoshimi Kikuchi ,  Osamu Kurahashi ,  Kenzo Yokozeki ,  Takashi Tanaka

发明人： Yoshimi Kikuchi ,  Osamu Kurahashi ,  Kenzo Yokozeki ,  Takashi Tanaka

IPC分类号： C12P2106

CPC分类号： C12P13/08

摘要： A method for producing a fermentative product by utilizing a microorganism, the method comprising culturing the microorganism in a medium to produce and accumulate the fermentative product in the medium, and collecting the fermentative product, wherein the microorganism expresses a heat shock protein derived from a hyperthermophilic archaeon strain KOD-1 in a cell of the microorganism by introduction of a gene coding for the heat shock protein.

摘要翻译： 一种通过利用微生物生产发酵产物的方法，所述方法包括在培养基中培养微生物以在培养基中产生和积累发酵产物，并收集发酵产物，其中微生物表达来源于超嗜热的热休克蛋白 通过引入编码热休克蛋白的基因，在微生物细胞中考古菌株KOD-1。

公开(公告)号：US06242228B1

公开(公告)日：2001-06-05

申请号：US09363189

申请日：1999-07-29

申请人： Masakazu Sugiyama ,  Naoto Tonouchi ,  Shunichi Suzuki ,  Kenzo Yokozeki

发明人： Masakazu Sugiyama ,  Naoto Tonouchi ,  Shunichi Suzuki ,  Kenzo Yokozeki

IPC分类号： C12P718

CPC分类号： C12N9/0006 ,  C12P7/18

摘要： Xylitol is produced by allowing xylitol dehydrogenase or cells instoduced with a DNA coding for xylitol dehydrogenase, which is a protein of the following (A) or (B) to act on D-xylulose, and collecting produced xylitol: (A) a protein which has the amino acid sequence of SEQ ID NO: 4; (B) a protein which has the amino acid sequence of SEQ ID NO: 4 including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and has xylitol dehydrogenase activity.

摘要翻译： 通过使木糖醇脱氢酶或用编码木糖醇脱氢酶的DNA（其是以下（A）或（B）的蛋白质）作用于D-木酮糖并收集产生的木糖醇的DNA产生木糖醇：（A） 具有SEQ ID NO：4的氨基酸序列;（B）具有SEQ ID NO：4的氨基酸序列的蛋白质，包括一个或几个氨基酸的取代，缺失，插入，添加或倒位，并且具有木糖醇 脱氢酶活性。