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    Recombinant DNA having hydantoinase gene and carbamylase gene and process for producing amino acid
    2.
    发明申请
    Recombinant DNA having hydantoinase gene and carbamylase gene and process for producing amino acid 审中-公开
    具有乙内酰脲酶基因和氨基甲酰酶基因的重组DNA及其生产氨基酸的方法

    公开(公告)号：US20050112729A1

    公开(公告)日：2005-05-26

    申请号：US10961116

    申请日：2004-10-12

    Applicant: Ikuo Kira ,  Yasuhiro Takenaka ,  Hiroyuki Nozaki ,  Kunihiko Watanabe

    Inventor： Ikuo Kira ,  Yasuhiro Takenaka ,  Hiroyuki Nozaki ,  Kunihiko Watanabe

    IPC: C12N1/21 ,  C12N9/80 ,  C12N9/86 ,  C12N15/71 ,  C12P13/04

    CPC classification number: C12N9/80 ,  C12N9/86 ,  C12N15/71 ,  C12P13/04

    Abstract: The object of the present invention is to prepare a recombinant DNA efficiently co-expressing genes of enzymes such as HHase, and to produce an amino acid efficiently from hydantoin by utilizing such a recombinant DNA. The present invention provides a recombinant DNA having base sequences encoding the following gene regions (I) and (II) incorporated therein in a predetermined direction and in a predetermined order to produce an amino acid efficiently: (I) a gene region containing a base sequence encoding a hydantoinase gene and a trp promoter sequence located upstream of the base sequence encoding the hydantoinase gene regulating expression of the hydantoinase gene, and (II) a gene region containing a base sequence encoding a carbamylase gene and a trp promoter sequence located upstream of the base sequence encoding the carbamylase gene regulating expression of the carbamylase gene.

    Abstract translation: 本发明的目的是制备有效共同表达诸如HHase的酶的基因的重组DNA，并通过利用这种重组DNA从乙内酰脲有效地产生氨基酸。 本发明提供一种具有编码下述基因区（I）和（II）的碱基序列的重组DNA，其特征在于，在规定的方向和规定的顺序中并入其中，以有效地产生氨基酸：（I）含有碱基序列 编码乙内酰脲酶基因和位于编码修饰乙内酰脲酶基因表达的乙内酰脲酶基因的碱基序列上游的trp启动子序列，以及（II）含有编码氨基甲酰化酶基因的碱基序列和位于上游的trp启动子序列的基因区域 编码调节氨基甲酰酶基因表达的氨基甲酰酶基因的碱基序列。

    Production method of D-alloisoleucine
    6.
    发明申请
    Production method of D-alloisoleucine 审中-公开
    D-异亮氨酸的生产方法

    公开(公告)号：US20050202542A1

    公开(公告)日：2005-09-15

    申请号：US10855349

    申请日：2004-05-28

    Applicant: Shogo Maruyama ,  Ikuo Kira ,  Tadashi Takemoto

    Inventor： Shogo Maruyama ,  Ikuo Kira ,  Tadashi Takemoto

    IPC: C12P13/04

    CPC classification number: C12P13/06

    Abstract: The present invention provides a production method of D-alloisoleucine, a protected compound thereof or a salt thereof, which is industrially useful, and which efficiently produces D-alloisoleucine having a high purity, a protected compound thereof and a salt thereof, and more particularly, a method including steps (a) to (c): (a) carbamoylating L-isoleucine to give L-(N-carbamoyl)isoleucine, (b) conducting intramolecular dehydration condensation of L-(N-carbamoyl)isoleucine to give L-5-sec-butylhydantoin, and (c) conducting an enzyme reaction of L-5-sec-butylhydantoin with hydantoin racemase (HRase), D-hydantoinase (D-HHase) and N-carbamoyl-D-amino acid hydrolase (D-CHase) to give D-alloisoleucine.

    Abstract translation: 本发明提供了在工业上有用的D-异亮氨酸，其保护的化合物或其盐的生产方法，并且其有效地生产具有高纯度的D-异亮氨酸，其保护的化合物及其盐，更特别地 ，（a）〜（c）的方法：（a）氨基甲酰化L-异亮氨酸得到L-（N-氨基甲酰基）异亮氨酸，（b）进行L-（N-氨基甲酰基）异亮氨酸的分子内脱水缩合，得到L 5-仲丁基乙内酰脲，（c）进行L-5-仲丁基乙内酰脲与乙内酰脲消旋酶（HRase），D-乙内酰脲酶（D-HHase）和N-氨基甲酰基-D-氨基酸水解酶 -CHase），得到D-异亮氨酸。

    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase
    7.
    发明申请
    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase 审中-公开
    二肽生产方法，其中使用的L-氨基酸酰胺水解酶，以及L-氨基酸酰胺水解酶的制备方法

    公开(公告)号：US20070042459A1

    公开(公告)日：2007-02-22

    申请号：US11249429

    申请日：2005-10-14

    Applicant: Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    Inventor： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    IPC: C12P21/06 ,  C12N9/48

    CPC classification number: C12P21/02 ,  C07K5/06026 ,  C12N9/48

    Abstract: A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.

    Abstract translation: 提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。 通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或 来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物 。 该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。

    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase
    9.
    发明授权
    Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase 失效
    二肽生产方法，其中使用的L-氨基酸酰胺水解酶，以及L-氨基酸酰胺水解酶的制备方法

    公开(公告)号：US07037673B2

    公开(公告)日：2006-05-02

    申请号：US10481087

    申请日：2002-07-26

    Applicant: Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    Inventor： Hiroyuki Nozaki ,  Ikuo Kira ,  Sonoko Suzuki ,  Kenzo Yokozeki

    IPC: C12P21/00 ,  C12N9/58

    CPC classification number: C12P21/02 ,  C07K5/06026 ,  C12N9/48

    Abstract: A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.

    Abstract translation: 提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。 通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或 来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物 。 该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。

    Mutant microorganism and method for producing peptide using the same
    10.
    发明申请
    Mutant microorganism and method for producing peptide using the same 审中-公开
    突变体微生物及其使用方法

    公开(公告)号：US20050176150A1

    公开(公告)日：2005-08-11

    申请号：US11050829

    申请日：2005-02-07

    Applicant: Ikuo Kira ,  Kenzo Yokozeki ,  Sonoko Suzuki ,  Yasuhiro Mihara ,  Yoshinori Hirao

    Inventor： Ikuo Kira ,  Kenzo Yokozeki ,  Sonoko Suzuki ,  Yasuhiro Mihara ,  Yoshinori Hirao

    IPC: C12N1/21 ,  C12N9/88 ,  C12N15/74

    CPC classification number: C12N9/88 ,  C12N15/74

    Abstract: The present invention provides a method for producing the peptides comprising: cultivating in medium the microorganisms wherein at least one gene selected from the group consisting of a gene encoding; aminoacylhistidine peptidase; a gene encoding leucylaminopeptidase; and a gene encoding isoaspartyldipeptidase, respectively; has been disrupted on the chromosome and wherein preferably transformed with the recombinant DNA, comprising polynucleotide encoding the proteins having peptide-synthesizing activity, mixing at least one of the cultivated microorganisms and the disrupted cells of the microorganisms with the carboxy and amine components for the peptide synthesis.

    Abstract translation: 本发明提供了一种生产肽的方法，包括：在培养基中培养微生物，其中至少一种选自下组的基因：基因编码; 氨酰基组氨酸肽酶; 编码亮氨酰氨基肽酶的基因; 和分别编码异天竺葵酰二肽酶的基因; 已经在染色体上被破坏，并且其中优选用重组DNA转化，包括编码具有肽合成活性的蛋白质的多核苷酸，将至少一种培养的微生物和微生物的破坏细胞与肽的羧基和胺组分混合 合成。

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