公开(公告)号：US20210356480A1

公开(公告)日：2021-11-18

申请号：US17331264

申请日：2021-05-26

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Livia Schiavinato Eberlin ,  Christina Ramires Ferreira ,  Allison Lisa Dill ,  Demian R. Ifa

IPC: G01N33/92 ,  H01J49/00 ,  H01J49/16

Abstract: The invention generally relates to mass spectral analysis. In certain embodiments, methods of the invention involve analyzing a lipid containing sample using a mass spectrometry technique, in which the technique utilizes a liquid phase that does not destroy native tissue morphology during analysis.

公开(公告)号：US20210293826A1

公开(公告)日：2021-09-23

申请号：US17342804

申请日：2021-06-09

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Zane Baird ,  Michael Pugia ,  Adam Hollerbach ,  Stephen Ayrton

IPC: G01N33/68 ,  G01N33/531 ,  G01N33/574

Abstract: The invention generally relates to charged mass label compositions and methods of use thereof for detecting a target analyte in a sample. In certain aspects, the invention provides a charged mass label composition including an affinity reagent, and a mass label precursor bound to the affinity reagent. The mass label precursor includes a label binding unit and a mass label. The label binding unit reversibly binds the mass label to the affinity reagent. The mass label includes a charge unit and a mass label unit having a pre-defined mass-to-charge-value in a mass spectrum.

公开(公告)号：US20210265155A1

公开(公告)日：2021-08-26

申请号：US17319474

申请日：2021-05-13

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Dalton Snyder ,  Amar S. Basu ,  Wen-Ping Peng

IPC: H01J49/42 ,  G01N27/62 ,  G01N27/622 ,  H01J49/00 ,  H01J49/16

Abstract: The invention generally relates to systems and methods for separating ions at about or above atmospheric pressure. In certain embodiments, the invention provides systems that include an ionization source that generates ions and an ion trap. The ion trap is maintained at about or above atmospheric pressure and includes a plurality of electrodes and at least one inlet configured to receive a gas flow and at least one outlet. The system is configured such that a combination of a gas flow and one or more electric fields produced by the electrodes separates the ions based on mass-to-charge ratio and sends the separated ions through the at least one outlet of the ion trap.

公开(公告)号：US10991564B2

公开(公告)日：2021-04-27

申请号：US16732568

申请日：2020-01-02

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Depanjan Sarkar ,  Thalappil Pradeep ,  Rahul Narayanan

IPC: H01J49/16 ,  H01J49/04 ,  H05K1/18 ,  H01J49/00

Abstract: The invention generally relates to mass spectrometry probes and systems for ionizing a sample. In certain embodiments, the invention provides a mass spectrometry probe including a substrate in which a portion of the substrate is coated with a material, a portion of which protrudes from the substrate.

公开(公告)号：US10964517B2

公开(公告)日：2021-03-30

申请号：US17014264

申请日：2020-09-08

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Ahmed Mohamed Hamid ,  Alan Keith Jarmusch ,  Zheng Ouyang

IPC: H01J49/04 ,  C12N13/00 ,  C12Q1/04 ,  H01J49/16

Abstract: The invention generally relates to systems and methods for mass spectrometry analysis of microorganisms in samples.

公开(公告)号：US20210050206A1

公开(公告)日：2021-02-18

申请号：US17070411

申请日：2020-10-14

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Jason Duncan ,  Joshua Wiley

IPC: H01J49/42 ,  H01J49/00

Abstract: The invention generally relates to ion traps and methods of use thereof. In certain embodiments, the invention provides a system that includes a mass spectrometer including an ion trap, and a central processing unit (CPU). The CPU has storage that is coupled to the CPU for storing instructions that when executed by the CPU cause the system to apply a constant radio frequency (RF) signal to the ion trap, and apply a first alternating current (AC) signal to the ion trap the frequency of which varies as a function of time.

公开(公告)号：US20210045662A1

公开(公告)日：2021-02-18

申请号：US17066727

申请日：2020-10-09

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Zheng Ouyang ,  Chien-hsun Chen ,  Ziqing Lin ,  Livia Schiavinato Eberlin

IPC: A61B5/1477 ,  A61B10/00 ,  H01J49/04 ,  A61M11/00 ,  A61M25/00 ,  H01J49/00 ,  H01J49/16 ,  H01J49/34 ,  G01N33/483

Abstract: The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

公开(公告)号：US10923338B2

公开(公告)日：2021-02-16

申请号：US16987594

申请日：2020-08-07

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Zane Baird ,  Wen-Ping Peng

IPC: H01J49/06 ,  H01J49/10 ,  H01J49/26 ,  H01J49/16

Abstract: The invention generally relates to apparatuses for focusing ions at or above ambient pressure and methods of use thereof. In certain embodiments, the invention provides an apparatus for focusing ions that includes an electrode having a cavity, at least one inlet within the electrode configured to operatively couple with an ionization source, such that discharge generated by the ionization source is injected into the cavity of the electrode, and an outlet. The cavity in the electrode is shaped such that upon application of voltage to the electrode, ions within the cavity are focused and directed to the outlet, which is positioned such that a proximal end of the outlet receives the focused ions and a distal end of the outlet is open to ambient pressure.

公开(公告)号：US20200381238A1

公开(公告)日：2020-12-03

申请号：US16885540

申请日：2020-05-28

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Zhenwei Wei ,  David G. Mclaren

IPC: H01J49/16 ,  H01J49/04

Abstract: The invention generally relates to systems including nanoelectrospray ionization emitters in a movable array format in which the emitters can be loaded, singly or simultaneously, through their narrow ends using a novel dip and go method based on capillary action, taking up sample from an array. The sample solutions in each emitter can be electrophoretically cleaned, singly or simultaneously, by creating an inductive electric field that moves interfering ions away from the narrow end of the capillary. Subsequent to cleaning, the emitters are supplied with an inductive electric field that causes electrospray into a mass spectrometer allowing mass analysis of the contents of the emitter.

公开(公告)号：US20200381237A1

公开(公告)日：2020-12-03

申请号：US16987594

申请日：2020-08-07

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Zane Baird ,  Wen-Ping Peng

IPC: H01J49/06 ,  H01J49/10 ,  H01J49/16 ,  H01J49/26

Abstract: The invention generally relates to apparatuses for focusing ions at or above ambient pressure and methods of use thereof. In certain embodiments, the invention provides an apparatus for focusing ions that includes an electrode having a cavity, at least one inlet within the electrode configured to operatively couple with an ionization source, such that discharge generated by the ionization source is injected into the cavity of the electrode, and an outlet. The cavity in the electrode is shaped such that upon application of voltage to the electrode, ions within the cavity are focused and directed to the outlet, which is positioned such that a proximal end of the outlet receives the focused ions and a distal end of the outlet is open to ambient pressure.