摘要:
A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO:12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.
摘要翻译:在HCV感染样品中扩增HCV核酸的方法包括通过两阶段PCR从样品中扩增与HCV RNA基因组互补的DNA模板的片段,其中第一阶段PCR使用第一外部引物 和第二外引物,第二阶段PCR采用第一内引物和第二内引物。 第一外引物的核苷酸序列包含SEQ ID NO:2所示的核苷酸序列; 或SEQ ID NO:9,其中任选地1,2或3个核苷酸是比SEQ ID NO:9的核苷酸更多的核苷酸。第二外部引物的核苷酸序列包含SEQ ID NO:3或4所示的核苷酸序列; 或如SEQ ID NO:10或11所示的核苷酸序列,其中任选1,2或3个核苷酸是与SEQ ID NO:10和11相同的其它核苷酸。第一内引物的核苷酸序列包含核苷酸序列 如SEQ ID NO:5所示; 或SEQ ID NO:12,其中任选地1,2或3个核苷酸是与SEQ ID NO:12相同的其它核苷酸。第二内引物的核苷酸序列包含SEQ ID NO:6或7所示的核苷酸序列 ; 或如SEQ ID NO:13或14所示的核苷酸序列,其中任选地1,2或3个核苷酸是与SEQ ID NO:13和14相同的其它核苷酸。
摘要:
An apparatus and method are disclosed for state sharing. A change module detects a change of a configuration state for at least one of a base and a detachable device. A connection module detects a connection between the base and the detachable device. The detachable device provides a display and a network connection for the base if the base and detachable device are connected. A synchronize module synchronizes the configuration state of the detachable device with the configuration state of the base in response to detecting the connection and detecting the change of configuration state.
摘要:
A device and method include detecting signal strength received at a mobile cellular communication device. A dropped call is detected between the device and another device as a function of detected signal strength. The dropped call is reconnected when detected signal strength reaches a selected first threshold.
摘要:
Disclosed are approaches for automatically forming a wireless connection for a computing device. In one embodiment, a method can include: determining that a first wireless connection for a computing device has been lost; receiving a browser indication from a browser running on the computing device that a second wireless connection is desired for the computing device; providing, in response to the browser indication, an automatic connection indication to a user of the computing device via a user interface; receiving an acceptance indication from the user interface in response to the automatic connection indication; and establishing the second wireless connection for the computing device in response to the acceptance indication from the user interface.
摘要:
A method for power management in handover between heterogeneous networks comprising: an MIH layer obtains power information and provides it for an MIH user layer; the MIH user layer determines the handover policy according to the power information. In various embodiments of the disclosure, the MIH layer triggers an event so that the MIH user layer can obtain power information, and select a lower-layer network connection automatically according to the current power status, thus implementing handover; the MIH user layer sends a query request to the MIH layer to obtain power consumption parameters from the network, and selects a lower-layer network connection according to the power consumption and the current power status, thus implementing handover and making more accurate and effective decisions in the handover.
摘要:
The invention broadly contemplates a computing system that offers high-speed recovery. The system is configured to offer such a high-speed solution via an instant roll back scheme. The system utilizes a virtualization to handle the system state and provide a rescue and recovery like solution without requiring a full system reboot. The system is configured to utilize virtualization, e.g. through the use of a hypervisor to track the fundamental system components. This enables the system to very quickly back the system up on a rolling basis via tracking atomic changes and offering the instant rollback at essentially any point following an atomic change, should common system difficulties be encountered.
摘要:
An apparatus and method are disclosed for power management. A monitor module monitors a connection between a base and a detachable device from the base and from the detachable device. The detachable device provides a display for the base if the detachable device and base are connected. A detection module detects a connection event selected from the group consisting of a detachable device connection to the base and a detachable device disconnection from the base.
摘要:
A device and method include detecting signal strength received at a mobile cellular communication device. A dropped call is detected between the device and another device as a function of detected signal strength. The dropped call is reconnected when detected signal strength reaches a selected first threshold.
摘要:
An ice sledding device includes a blade mounted on a main body support. The main body support of the ice sledding device is formed with an inclined top surface for supporting the player during the normal user of the ice sledding device.
摘要:
A hypervisor boots all guest operating systems needed to setup/update server components as detected by an update utility. The update utility, after detecting server components, is booted into each guest O.S. so that the components are updated in parallel without having to sequentially boot the utility into a guest O.S., update its component, then shut down and re-boot into another guest O.S.