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    Fluorescent labeling reagents with multiple donors and acceptors
    73.
    发明申请
    Fluorescent labeling reagents with multiple donors and acceptors 审中-公开
    具有多个供体和受体的荧光标记试剂

    公开(公告)号：US20050260593A1

    公开(公告)日：2005-11-24

    申请号：US10528862

    申请日：2003-09-25

    申请人： Shiv Kumar ,  Chung-Yuan Chen

    发明人： Shiv Kumar ,  Chung-Yuan Chen

    IPC分类号： C07D311/88 ,  C07H21/04 ,  C12Q1/68 ,  C07D45/14

    CPC分类号： C07H21/04

    摘要： Disclosed is a novel class of fluorescent resonance energy transfer (FRET) labelling reagents, based on and synthesised from easily prepared dye building blocks. The labelling reagents are in the form of “cassettes” which enable their attachment to a wide variety of biological and other materials. A labelling reagent comprises at least two fluorescent dye moieties covalently linked via a linker group and optionally having a target bonding group for attaching the reagent to a target. The energy transfer labelling reagents may be bound to target materials through covalent or non-covalent attachment. The dyes are selected so that the emission spectrum of a first (or donor) dye overlaps the absorption spectrum of a second dye, thereby allowing energy transfer to occur between the dyes. The dye building blocks are 4′, 5′-bis-aminomethyl-fluorescein and/or its 5(6)-carboxylic acid and having the structure (1). In addition to the embodiment of the invention which includes a single donor and a single acceptor fluorochrome, the fluorescent energy transfer labelling reagents according to the invention may further comprise one or more third fluorochromes each having third absorption and emission spectra covalently attached to said first or second fluorochromes.

    摘要翻译： 公开了一种新型的荧光共振能量转移（FRET）标记试剂，基于并由易于制备的染料积木合成。 标记试剂是“盒式”的形式，使其能够连接到各种各样的生物和其他材料。 标记试剂包含至少两个通过连接基团共价连接的荧光染料部分，并且任选地具有用于将试剂连接到靶上的目标结合基团。 能量转移标记试剂可以通过共价或非共价连接与目标材料结合。 选择染料使得第一（或供体）染料的发射光谱与第二染料的吸收光谱重叠，从而允许在染料之间发生能量转移。 染料结构单元是4'，5'-双 - 氨基甲基 - 荧光素和/或其5（6） - 羧酸并具有结构（1）。 除了包括单一供体和单受体荧光染料的本发明的实施方案之外，根据本发明的荧光能量转移标记试剂还可以包含一种或多种第三种荧光染料，每种第三种荧光染料具有共价连接到所述第一或第 第二荧光染料。

    Colorant compounds
    74.
    发明申请
    Colorant compounds 有权
    着色剂化合物

    公开(公告)号：US20050228183A1

    公开(公告)日：2005-10-13

    申请号：US11141898

    申请日：2005-06-01

    申请人： Jeffery Banning ,  Bo Wu ,  James Duff ,  Wolfgang Wedler ,  Jule Thomas ,  Randall Bridgeman

    发明人： Jeffery Banning ,  Bo Wu ,  James Duff ,  Wolfgang Wedler ,  Jule Thomas ,  Randall Bridgeman

    IPC分类号： C09B11/28 ,  C09B11/12 ,  C09B11/24 ,  C09D11/00 ,  C07D45/14

    CPC分类号： C09D11/34 ,  C09B11/12 ,  C09B11/24

    摘要： Compounds of the formula wherein M is either (1) a metal ion having a positive charge of +p wherein p is an integer which is at least 2, said metal ion being capable of forming a compound with at least two chromogen moieties, or (2) a metal-containing moiety capable of forming a compound with at least two chromogen moieties, z is an integer representing the number of chromogen moieties associated with the metal and is at least 2, and R1, R2, R3, R4, R5, R6, R7, a, b, c, d, Y, Q−, A, and CA are as defined herein.

    摘要翻译： 下式的化合物其中M是（1）具有正电荷+ p的金属离子，其中p是至少为2的整数，所述金属离子能够形成具有至少两个色原子部分的化合物，或（ 2）能够形成具有至少两个色原体部分的化合物的含金属部分，z是表示与金属相关的染色体部分的数目的整数，并且至少为2，R 1， R 2，R 3，R 4，R 5，R 6， R 7，a，b，c，d，Y，Q - ，A和CA如本文所定义。