公开(公告)号：US11053283B2

公开(公告)日：2021-07-06

申请号：US16386628

申请日：2019-04-17

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Ola Lind ,  Bjorn Noren ,  Ronnie Palmgren

IPC: C07K1/22 ,  C07K16/00 ,  C07K16/06 ,  C07K1/14

Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.

公开(公告)号：US20210170303A1

公开(公告)日：2021-06-10

申请号：US17169699

申请日：2021-02-08

Applicant: Cytiva BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: B01D15/10 ,  B01D15/18 ,  G01N30/46 ,  B01D15/20 ,  G01N30/60 ,  G01N30/02 ,  G01N30/56

Abstract: Disclosed herein is a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column.

公开(公告)号：US11014069B2

公开(公告)日：2021-05-25

申请号：US15769400

申请日：2016-10-28

Applicant: Cytiva BioProcess R&D AB

Inventor： Nils Norrman ,  Mattias Algotsson ,  Stefan D. Eriksson

IPC: B01J20/24 ,  B01J20/29 ,  B01J20/30 ,  B01J20/28 ,  B01J20/26 ,  B01J20/32 ,  B01D15/38 ,  B01D15/36

Abstract: The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran-containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media.

公开(公告)号：US20210147577A1

公开(公告)日：2021-05-20

申请号：US17149830

申请日：2021-01-15

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Tomas Bjorkman ,  Mats Ander

IPC: C07K16/46 ,  B01J20/289 ,  B01J20/32 ,  B01D15/38 ,  C07K16/06 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00

Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.

公开(公告)号：US20210113940A1

公开(公告)日：2021-04-22

申请号：US17125645

申请日：2020-12-17

Applicant: Cytiva BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: B01D15/14 ,  B01D15/18 ,  B01D15/22 ,  G01N30/46 ,  G01N30/60

Abstract: A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

公开(公告)号：US20210077920A1

公开(公告)日：2021-03-18

申请号：US17102801

申请日：2020-11-24

Applicant: Cytiva BioProcess R&D AB

Inventor： Klaus Gebauer ,  Manoj Kumar Ramakrishna ,  Daniel Salomonsson ,  Per Lofving

IPC: B01D15/22 ,  B01D15/18 ,  G01N30/46 ,  G01N30/60 ,  F16L55/00

Abstract: A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b) connected in a rigid housing (21; 61), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules in the rigid housing are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.