摘要:
The present invention relates to polynucleotides corresponding to the rpoB gene and which encode the &bgr;-subunit of RNA polymerase B, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having activity of the &bgr;-subunit of RNA polymerase B.
摘要翻译:本发明涉及对应于rpoB基因并编码RNA聚合酶B的β-亚单位的多核苷酸,产生L-氨基酸的方法,以及筛选编码具有RNA聚合酶的β-亚基活性的蛋白质的多核苷酸的方法 B.
摘要:
The invention provides nucleotide sequences from Coryneform bacteria which code for the RodA protein and a process for the fermentative preparation of amino acids using bacteria in which the rodA gene is enhanced.
摘要:
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the sigH gene, and a host-vector system having a coryneform host bacterium in which the sigH gene is present in attenuated form and a vector which carries at least the sigH gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及具有编码sigH基因的多核苷酸序列的分离的多核苷酸,以及具有其中sigH基因以减毒形式存在的棒状细胞宿主细菌的宿主 - 载体系统和至少携带sigH基因的载体 至SEQ ID No 1,以及使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
The invention provides a feedstuff additive based on a fermentation broth which is obtained by the fermentation of D-pantothenic acid producing microorganisms and contains one or more salts of D-pantothenic acid, selected from the group of sodium, potassium, ammonium, magnesium or calcium salts.
摘要:
The invention provides nucleotide sequences of the gpm gene which encode phosphoglycerate mutase, and fermentation processes for the preparation of amino acids, especially L-lysine, using corynebacteria wherein the gpm gene is amplified.
摘要:
The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译:本发明涉及分离的多核苷酸,其包含多核苷酸序列,所述多核苷酸序列选自a)多核苷酸,其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70% b)编码多肽的多核苷酸,其包含与SEQ ID No.2的氨基酸序列至少70%相同的氨基酸序列,c)与a)的多核苷酸互补的多核苷酸, 或b),和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少有citA基因 以减毒形式存在,并且使用包含根据本发明的序列的多核苷酸作为杂交探针。
摘要:
A process for the production of an L-amino acid wherein coryneform bacteria (e.g. Coryneform glutamicum) in which expression of the mqo gene coding for malate quinone oxidoreductase is attenuated are fermented to produce a desired amino acid, and the amino acid is concentrated in the medium or cells and isolated. Optionally, further genes in the biosynthetic pathway of the desired amino acid are enhanced, and/or metabolic pathways that reduce formation of the amino acid are suppressed.
摘要:
Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.
摘要翻译:分离的多核苷酸,其包含选自以下的多核苷酸序列:a)与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸,b)多核苷酸, 其编码多肽,其包含与SEQ ID No.2的氨基酸序列至少70%的程度相同的氨基酸序列,c)与a)或b)的多核苷酸互补的多核苷酸, 和d)包含a),b)或c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状细菌发酵制备L-氨基酸的方法,其中至少lysR2基因存在于减毒 形式,以及使用多核苷酸序列作为杂交探针。
摘要:
The invention provides nucleotide sequences from Coryneform bacteria coding for the dep33 efflux protein and a process for the fermentative preparation of amino acids using bacteria in which the dep33 efflux protein is attenuated.
摘要:
The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the mikE17 gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.