公开(公告)号：US07270952B2

公开(公告)日：2007-09-18

申请号：US10254201

申请日：2002-09-24

申请人： Xing Su ,  Selena Chan ,  Tae-Woong Koo ,  Mineo Yamakawa ,  Andrew A. Berlin

发明人： Xing Su ,  Selena Chan ,  Tae-Woong Koo ,  Mineo Yamakawa ,  Andrew A. Berlin

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6825 ,  B82Y5/00 ,  G01N33/54373 ,  G01N2800/52 ,  Y10S977/924 ,  C12Q2565/501

摘要： The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

公开(公告)号：US20070153266A1

公开(公告)日：2007-07-05

申请号：US11325832

申请日：2005-12-30

申请人： Tae-Woong Koo ,  Selena Chan

发明人： Tae-Woong Koo ,  Selena Chan

IPC分类号： G01J3/44 ,  G01N21/64 ,  G01N21/65

CPC分类号： G01J3/10 ,  G01J3/02 ,  G01J3/0256 ,  G01J3/453 ,  G01N21/64 ,  G01N21/658

摘要： Embodiments of the present invention provide a miniaturized spectroscopy system comprising a light source fabricated from porous silicon. Porous silicon light emitting devices can provide tunable, narrow, and directional luminescence. Advantageously, a porous silicon light source can be integrated into a silicon wafer based device thus simplifying the manufacture of a miniaturized spectros copy system and lab-on-a-chip type devices employing spectroscopic detection.

摘要翻译： 本发明的实施例提供一种包括由多孔硅制造的光源的小型化光谱系统。 多孔硅发光器件可以提供可调谐的，窄的和定向的发光。 有利地，多孔硅光源可以集成到基于硅晶片的器件中，从而简化了使用光谱检测的小型化光谱复制系统和实验室上的芯片型器件的制造。

公开(公告)号：US06972173B2

公开(公告)日：2005-12-06

申请号：US10099287

申请日：2002-03-14

申请人： Xing Su ,  Selena Chan ,  Andrew A. Berlin ,  Tae-Woong Koo ,  Narayan Sundararajan ,  Mineo Yamakawa

发明人： Xing Su ,  Selena Chan ,  Andrew A. Berlin ,  Tae-Woong Koo ,  Narayan Sundararajan ,  Mineo Yamakawa

IPC分类号： G01N21/65 ,  B01L3/00 ,  C12M1/00 ,  C12Q1/68 ,  G01N33/543 ,  C07H21/02 ,  C12P19/34

CPC分类号： C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6872 ,  C12Q2565/632 ,  C12Q2563/155 ,  C12Q2521/319

摘要： The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid 13. Exonuclease 15 treatment of the labeled nucleic acid 13 results in the release of labeled nucleotides 16, 130, which are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides 16, 130 released from a nucleic acid 13 by exonuclease 15 treatment are covalently cross-linked to silver or gold nanoparticles 140 and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus 10, 100, 210 for nucleic acid sequencing.

摘要翻译： 本文公开的方法和装置涉及通过增强拉曼光谱进行的核酸测序。 在本发明的某些实施方案中，核苷酸在掺入核酸13之前共价连接到拉曼标记上。 标记核酸13的核酸外切酶15处理导致通过拉曼光谱法检测的标记核苷酸16,130的释放。 在本发明的替代实施方案中，通过核酸外切酶15处理从核酸13释放的核苷酸16,130共价交联到银或金纳米颗粒140上，并通过表面增强拉曼光谱（SERS），表面增强共振拉曼光谱（SERRS ）和/或相干的反斯托克斯拉曼光谱（CARS）。 本发明的其它实施方案涉及用于核酸测序的装置10,100,210。

公开(公告)号：US20050221510A1

公开(公告)日：2005-10-06

申请号：US11134749

申请日：2005-05-19

申请人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

发明人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

IPC分类号： G01N21/65 ,  G01N33/553

CPC分类号： G01N21/658

摘要： The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.

公开(公告)号：US20050221509A1

公开(公告)日：2005-10-06

申请号：US11134747

申请日：2005-05-19

申请人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

发明人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

IPC分类号： G01N21/65 ,  G01N33/553

CPC分类号： G01N21/658

摘要： The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.

摘要翻译： 本发明基于这样的发现：本文所述的用于生产金属胶体的方法导致胶体表现出增加的信号增强和生物分子的SERS检测的再现性。 因此，使用本发明的方法，可以以更高的灵敏度和可靠性检测各种各样的生物分子。

公开(公告)号：US20050196876A1

公开(公告)日：2005-09-08

申请号：US10748390

申请日：2003-12-29

申请人： Selena Chan ,  Tae-Woong Koo

发明人： Selena Chan ,  Tae-Woong Koo

IPC分类号： G01N21/65 ,  G01N33/543 ,  C12M1/34 ,  G01N33/551

CPC分类号： G01N33/54373 ,  G01N21/6428 ,  G01N21/65 ,  G01N21/658

摘要： The invention provides methods used to analyze the contents of a biological sample, such as blood serum, with cascade Raman sensing. A fluorescence producing nanoporous biosensor having probes that bind specifically to known analytes is contacted with a biological sample and one or more bound complexes coupled to the porous semiconductor structure are formed. The bound complexes are contacted with a Raman-active probe that binds specifically to the bound complexes and the biosensor is illuminated to generate fluorescent emissions from the biosensor. These fluorescent emissions generate Raman signals from the bound complexes. The Raman signals produced by the bound complexes are detected and the Raman signal associated with a bound protein-containing analyte is indicative of the presence of the protein-containing compound in the sample. The invention methods are useful to provide a protein profile of a patient sample. The invention also provides detection systems useful to practice the invention methods.

摘要翻译： 本发明提供了利用级联拉曼检测来分析生物样品如血清的含量的方法。 具有特异性结合已知分析物的探针的产生荧光的纳米多孔生物传感器与生物样品接触，并形成耦合到多孔半导体结构的一个或多个结合复合物。 将结合的复合物与与结合的复合物特异性结合的拉曼活性探针接触，并且生物传感器被照射以产生来自生物传感器的荧光发射。 这些荧光发射从结合的复合物产生拉曼信号。 检测由结合的复合物产生的拉曼信号，并且与结合含蛋白质的分析物相关的拉曼信号指示样品中含蛋白质的化合物的存在。 本发明方法可用于提供患者样品的蛋白质谱。 本发明还提供了用于实施本发明方法的检测系统。