公开(公告)号：US5071962A

公开(公告)日：1991-12-10

申请号：US531317

申请日：1990-05-31

申请人： Richard P. Morrison ,  Harlan D. Caldwell

发明人： Richard P. Morrison ,  Harlan D. Caldwell

IPC分类号： A61K38/00 ,  C07K14/295 ,  C07K16/12 ,  C12N15/31

CPC分类号： C07K14/295 ,  C07K16/125 ,  A61K38/00 ,  Y10S530/808 ,  Y10S530/809

摘要： The present invention relates to novel polypeptides comprising a unique "chlamydial-specific" primary structural conformation and one or more of the biological properties of eukaryotic or prokaryotic stress-response proteins which are characterized by being the expressed products of an endogenous or exogenous DNA sequence in a eukaryotic or prokaryotic host cell. Sequences coding for part or all of the amino acid residues of the chlamydial HypA or HypB protein or for analogs thereof may be incorporated into autonomously replicating vectors employed to transform or transfect suitable procaryotic or eukaryotic host cells such as bacteria or vertebrate cells in culture. The HypB protein is a member of the family of stress response proteins referred to as HSP60. Products of expression of the DNA sequences display the identical physical, immunological, and histological properties as the chlamydial proteins isolated from natural, non-recombinant, organisms.

摘要翻译： 本发明涉及包含独特的“衣原体特异性”初级结构构象和一种或多种真核或原核应激蛋白的生物学特性的新型多肽，其特征在于内源或外源DNA序列的表达产物 真核或原核宿主细胞。 编码衣原体HypA或HypB蛋白的部分或全部氨基酸残基或其类似物的序列可以并入到用于转化或转染合适的原核或真核宿主细胞如培养物中的细菌或脊椎动物细胞的自主复制载体中。 HypB蛋白是称为HSP60的应激反应蛋白家族的成员。 DNA序列的表达产物显示与从天然非重组生物分离的衣原体蛋白质相同的物理，免疫学和组织学性质。

公开(公告)号：US20240156937A1

公开(公告)日：2024-05-16

申请号：US18278118

申请日：2022-04-20

申请人： UNM RAINFOREST INNOVATIONS

发明人： AMANDA L. COLLAR ,  KATHRYN MARIE FRIETZE

IPC分类号： A61K39/118 ,  A61K47/69 ,  A61P37/04 ,  C07K14/005 ,  C07K14/295 ,  C12N7/00 ,  A61K39/00

CPC分类号： A61K39/118 ,  A61K47/6901 ,  A61P37/04 ,  C07K14/005 ,  C07K14/295 ,  C12N7/00 ,  A61K2039/5258 ,  A61K2039/575 ,  C12N2795/18122 ,  C12N2795/18123 ,  C12N2795/18134 ,  C12N2795/18171

摘要： The present invention is directed virus-like particles (VLPs) which are useful in immunogenic compositions and vaccines epitopes mediating protection are disclosed. Immunogenic peptides are identified and are displayed on virus-like particles, especially Qbeta, MS2, or AP205 VLPs which provide a potent immunogenic response in a patient or subject and enhanced protection from Ct infection in a patient or subject. Pharmaceutical compositions and vaccines are disclosed as are methods for providing an immunogenic response and/or vaccinating a patient or subject against Chlamydia trachomatis infections.

公开(公告)号：US20240131138A1

公开(公告)日：2024-04-25

申请号：US18269113

申请日：2021-12-22

申请人： Institut National de la Santé et de la Recherche Médicale (INSERM) ,  Assistance Publique - Hopitaux de Paris ,  Baylor Research Institute ,  Universite Paris Est Creteil Val De Marne

发明人： Yves LEVY ,  Sylvain CARDINAUD ,  Mireille CENTLIVRE ,  Lydie DIEUDONNE ,  Sandra ZURAWSKI ,  Gérard ZURAWSKI

IPC分类号： A61K39/118 ,  A61P31/04 ,  C07K14/295 ,  C07K16/28

CPC分类号： A61K39/118 ,  A61P31/04 ,  C07K14/295 ,  C07K16/2851 ,  C07K16/2878 ,  A61K2039/505

摘要： Chlamydiae are intracellular bacterial pathogens responsible for a variety of infections. The inventors produced an antibody that is directed against a surface antigen (i.e., CD40) of an antigen presenting cell (i.e., dendritic cell) wherein the heavy chain and/or light chain is conjugated to the MOMP VS4 domain of Chlamydia trachomatis for its use as vaccine.

公开(公告)号：US20240124532A1

公开(公告)日：2024-04-18

申请号：US18274848

申请日：2022-01-28

申请人： Institut National de la Santé et de la Recherche Médicale (INSERM) ,  Assistance Publique - Hopitaux de Paris ,  Universite Paris Est Creteil Val De Marne ,  Baylor Research Institute

发明人： Yves LEVY ,  Sandra ZURAWSKI ,  Gérard ZURAWSKI ,  Mireille CENTLIVRE ,  Lydie DIEUDONNE ,  Sylvain CARDINAUD

IPC分类号： C07K14/295 ,  A61K39/118 ,  C07K16/28

CPC分类号： C07K14/295 ,  A61K39/118 ,  C07K16/2878 ,  C07K2319/33 ,  C07K2319/40

摘要： Chlamydiae are intracellular bacterial pathogens responsible for a variety of infections. The inventors have set up candidate vaccines against Chlamydia trachomatis. In particular, the inventors have identified specific epitopes to be included in vaccine candidates thanks to in silico analysis of the amino-acid sequence of these proteins to map predicted MHC-I and -II epitopes by online software (NetMHC-4.0 and NetMHCII-2.3) and peptide binding prediction software. B cell epitopes were also mapped using online software (BepiPred-2.0 and Discotope). Finally, the inventors have generated some specific CD40 or Langerin antibodies comprising one or more identified epitope(s) of the present invention and that are suitable for vaccine purposes. Therefore, the present invention relates to Chlamydia trachomatis (Ct) antigenic polypeptides and uses thereof for vaccine purposes.

公开(公告)号：US20220378892A1

公开(公告)日：2022-12-01

申请号：US17810406

申请日：2022-07-01

申请人： Merck Sharp & Dohme LLC

发明人： Lan Zhang ,  Zhiyun Wen ,  Craig T. Przysiecki ,  Puneet Khandelwal ,  Ping Qiu

IPC分类号： A61K39/02 ,  C07K14/295 ,  A61P31/04 ,  C12N15/70

摘要： The present invention relates to methods for the recombinant expression of chlamydia major outer membrane protein (MOMP) comprising transforming a population of E. coli host cells with an expression vector comprising a nucleic acid molecule that encodes chlamydia MOMP and encodes a leader sequence for targeting the MOMP to the outer membrane of the cell, wherein the nucleic acid molecule is operatively linked to a promoter. The method of the invention allows expression of MOMP in the outer membrane of the cell, which leads to protein folding that is more like native MOMP relative to a MOMP protein that is expressed intracellularly. Also provided by the invention are uses of the recombinant MOMP in pharmaceutical compositions and methods for the treatment and/or prophylaxis of chlamydia infection and/or the effects thereof.

公开(公告)号：US20220195013A1

公开(公告)日：2022-06-23

申请号：US17693785

申请日：2022-03-14

申请人： THROMBOSIS RESEARCH INSTITUTE

发明人： Xinjie Lu ,  Vijay Kakkar

IPC分类号： C07K14/775 ,  A61K39/00 ,  A61K39/118 ,  C07K14/195 ,  C07K14/35 ,  C07K14/295 ,  C07K14/47 ,  C07K14/705

摘要： The invention relates to multiple epitope constructs, immunogenic and vaccine compositions comprising recombinant molecules presenting inserted multiple and different epitopes from a variety of antigens. The antigenic determinants being associated with different pathways leading to atherosclerosis. In particular, the invention relates to such compositions for eliciting an immune response against antigens and pathogens involved in the development of atherosclerosis. The invention includes inter alia methods of treating and/or preventing the disease and recombinant protein products.

公开(公告)号：US11332515B2

公开(公告)日：2022-05-17

申请号：US15546858

申请日：2016-01-25

申请人： THROMBOSIS RESEARCH INSTITUTE ,  Lord Kakkar

发明人： Vijay Kakkar ,  Xinjie Lu

IPC分类号： C07K14/775 ,  A61K39/00 ,  A61K39/118 ,  C07K14/195 ,  C07K14/35 ,  C07K14/295 ,  C07K14/47 ,  C07K14/705

摘要： The invention relates to multiple epitope constructs, immunogenic and vaccine compositions comprising recombinant molecules presenting inserted multiple and different epitopes from a variety of antigens. The antigenic determinants being associated with different pathways leading to atherosclerosis. In particular, the invention relates to such compositions for eliciting an immune response against antigens and pathogens involved in the development of atherosclerosis the invention includes inter alia methods of treating and/or preventing the disease and recombinant protein products.

公开(公告)号：US20190282685A1

公开(公告)日：2019-09-19

申请号：US16260554

申请日：2019-01-29

申请人： GLAXOSMITHKLINE BIOLOGICALS, S.A.

发明人： Guido GRANDI ,  Renata Maria GRIFANTINI ,  Oretta FINCO

IPC分类号： A61K39/118 ,  G01N33/569 ,  A61K39/395 ,  C07K14/295 ,  C07K16/12

摘要： The invention provides Chlamydia antigens for use in the treatment, prevention and/or diagnosis of Chlamydia infection. In particular, the invention provides antigens CT733, CTI 53, CT601, CT279, CT443, CT372, CT456, CT381, CT255, CT341, CT716, CT745, CT387, CT812, CT869, CT166, CT175, CT163, CT214, CT721, CT127, CT043, CT823 and/or CT600 from C. trachomatis for the treatment, prevention or diagnosis of Chlamydia infection.

公开(公告)号：US20190046626A1

公开(公告)日：2019-02-14

申请号：US15527789

申请日：2015-11-16

申请人： Merck Sharp & Dohme Corp.

发明人： Lan Zhang ,  Zhiyun Wen ,  Craig T. Przysiecki ,  Puneet Khandelwal ,  Ping Qiu

IPC分类号： A61K39/02 ,  C07K14/295 ,  C12N15/70 ,  A61P31/04

CPC分类号： A61K39/02 ,  A61K2039/54 ,  A61K2039/55511 ,  A61P31/04 ,  C07K14/295 ,  C12N15/70

摘要： The present invention relates to methods for the recombinant expression of chlamydia major outer membrane protein (MOMP) comprising transforming a population of E. coli host cells with an expression vector comprising a nucleic acid molecule that encodes chlamydia MOMP and encodes a leader sequence for targeting the MOMP to the outer membrane of the cell, wherein the nucleic acid molecule is operatively linked to a promoter. The method of the invention allows expression of MOMP in the outer membrane of the cell, which leads to protein folding that is more like native MOMP relative to a MOMP protein that is expressed intracellularly. Also provided by the invention are uses of the recombinant MOMP in pharmaceutical compositions and methods for the treatment and/or prophylaxis of chlamydia infection and/or the effects thereof.

公开(公告)号：US20180021423A1

公开(公告)日：2018-01-25

申请号：US15551829

申请日：2016-02-19

申请人： Board of Regents, The University of Texas System

发明人： Guangming Zhong

IPC分类号： A61K39/118 ,  C12N1/36 ,  C07K14/295

CPC分类号： A61K39/118 ,  A61K2039/522 ,  C07K14/295 ,  C12N1/36

摘要： The present invention provides Chlamydia organisms and compositions and methods of use in the treatment/prevention of chlamydial infection in a subject, for eliciting an immune response in a subject and for use as vectors.