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公开(公告)号：US4294924A
公开(公告)日：1981-10-13
申请号：US123966
申请日：1980-02-25
CPC分类号： C12M23/02 , C12M23/22 , C12M23/38 , C12M23/44 , C12M23/46 , C12M25/14 , C12M41/36 , C12Q1/06 , Y10S435/801
摘要： A container for growing anaerobic microorganisms having a cone-shaped dish and matching cone-shaped cover that define between them a prescribed volume. An overflow trough surrounds the dish, and the peripheries of the dish and cover seal together by excess agar medium carrying the organisms squeezed from the volume as the cover is applied to the dish. The colonies of organisms can be viewed either through the dish or cover because each is transparent.
摘要翻译： 一种用于生长厌氧微生物的容器，其具有在其间限定规定体积的锥形盘和匹配的锥形盖。 溢流槽围绕着盘子，并且当盖子被施加到盘子上时，盘子和盖子的周边通过过量的琼脂培养基密封在一起，该琼脂培养基携带从体积挤压的生物体。 生物体的殖民地可以通过盘子或盖子观看，因为每个都是透明的。
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公开(公告)号：US4280000A
公开(公告)日：1981-07-21
申请号：US110203
申请日：1980-01-07
摘要： A method of obtaining mold spore material useful for inhalant allergy comprising growing a mold which includes mold spores and mycelia on a medium which supports growth of the mold. A porous barrier is provided between the medium and at least some of the mold so that a first group of the mold spores and mycelia lies on the side of the barrier remote from the medium and a second group of mycelia lies in the medium and on the other side of the barrier. After optimum growth of the mold has occurred, the barrier and the first group of mold spores and mycelia are removed from the medium and allowed to dry. Subsequently, at least some of the first group of mold spores and mycelia are removed from the barrier so that the step of removing separate the first group of mold spores and mycelia from the second group of mycelia. Following drying, the removed mold spores and mycelia are sieved to separate the mold spores from most of the mycelia.
摘要翻译： 获得用于吸入性过敏的霉菌孢子材料的方法包括在支持模具生长的培养基上生长包括霉菌孢子和菌丝体的模具。 在介质和模具中的至少一些之间提供多孔阻挡物，使得第一组模具孢子和菌丝体位于远离培养基的屏障侧上，而第二组菌丝体位于培养基中，并且在 屏障的另一面。 发生模具的最佳生长后，从培养基中取出阻隔物和第一组霉菌孢子和菌丝体，使其干燥。 随后，将第一组模具孢子和菌丝体中的至少一些从屏障中移除，使得从第二组菌丝体中分离出第一组霉菌孢子和菌丝体的步骤。 干燥后，将去除的霉菌孢子和菌丝体筛分，以从大部分菌丝体中分离霉菌孢子。
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公开(公告)号：US4271270A
公开(公告)日：1981-06-02
申请号：US63952
申请日：1979-08-06
申请人： Patricia A. Lukacsek
发明人： Patricia A. Lukacsek
摘要： An apparatus for culturing and examination of fungi is configured to maintain the sterility of a culture medium and a cover slip until ready for use in accordance with a method which cultures the fungi in an environment which remains undisturbed during microscopic examination of the cultured fungi.
摘要翻译： 配置真菌培养和检查的装置被配置为保持培养基和盖滑动的不育性，直到准备使用，根据在显微镜检查培养的真菌期间保持不受干扰的环境中的真菌培养的方法。
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公开(公告)号：US4212949A
公开(公告)日：1980-07-15
申请号：US935271
申请日：1978-08-21
申请人： Valentin G. Kozhemyakin , Kalust A. Kaluniants , Lidia S. Losyakova , Leonid I. Golger , Leonid F. Ivanov , Raisa G. Kozlova
发明人： Valentin G. Kozhemyakin , Kalust A. Kaluniants , Lidia S. Losyakova , Leonid I. Golger , Leonid F. Ivanov , Raisa G. Kozlova
摘要： An apparatus for cultivating microorganisms on a granular medium comprises a vertical cylindrical vessel with a top port for charging the seeded granular medium and a bottom port for discharging the culture. The vessel is separated, heightwise, into sections by horizontal partitions, each section being provided with a tube for letting in an aerating flow of air and a tube for letting out the flow with metabolites. Each partition is composite and made up of partially overlapping circular sectors with perforation in the form of slots.The sectors are provided with a means for shifting them so that when the medium is being charged into a section, all sectors form a solid plate, while, during discharge of the culture from the section, the sectors are shifted so that a free downward passage for the culture is provided.The sector shifting means is located under the partition, relative to the medium, and provides for shifting of the sectors in a horizontal plane. Coaxially arranged with the section is a loosening means whose rake interacts with the culture to keep it loose.
摘要翻译： 用于在颗粒介质上培养微生物的装置包括垂直圆柱形容器，其具有用于对种子颗粒状介质进行充电的顶部端口和用于排出培养物的底部端口。 容器通过水平隔板分开，高度地分成多个部分，每个部分设置有一个管子，用于使空气通气并且用代谢物排出流动管。 每个分区是复合的，并且由部分重叠的圆形部分组成，其中穿孔形式为槽。 这些部门被提供了一种移动它们的手段，使得当介质被充入一个部分时，所有扇区形成一个固体板，而在从该部分排出培养物的过程中，这些扇区被移动，使得自由下降通道 为文化提供。 扇形移动装置相对于介质位于隔板下方，并且在水平面中提供扇区的移位。 与该部分同轴布置的是松散的手段，其耙子与文化相互作用以使其松动。
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85.发明授权Article for growing cultures in a body cavity in the presence of gas, and package for the article 失效用于在气体存在下在体腔内培养文化的文章，并为文章包装
公开(公告)号：US4104127A
公开(公告)日：1978-08-01
申请号：US698470
申请日：1976-06-18
申请人： Louis Bucalo
发明人： Louis Bucalo
CPC分类号： A61B10/02 , A61F13/206 , C12M21/00 , C12M41/34 , Y10S435/81
摘要： Cultures of microorganisms can be grown in a body cavity by situating therein a nutrient on a suitable carrier while maintaining the nutrient exposed to a predetermined gaseous atmosphere, so that microorganisms which require a particular atmosphere can grow directly in the body cavity on the nutrient. After a time sufficient for culture growth the carrier with the nutrient thereon are removed from the body cavity and inspected, for example, under a microscope with suitable dyes being used to stain the growth for rendering the latter easily visible. The carrier for the nutrient may take the form of a plastic sheet material having small protuberances which are thicker than a nutrient layer carried by the plastic sheet material and which serve to maintain apart from each other surfaces of the plastic and nutrient which otherwise might engage each other to prevent exposure of the nutrient to the predetermined atmosphere. The article is preferably packaged in a container which has in its interior sufficient moisture to prevent drying of the nutrient, the interior of the container being closed off from the outer atmosphere by the sheet material which is used for the container.
摘要翻译： 微生物的培养物可以通过在体内将营养物置于合适的载体上，同时保持暴露于预定的气体气氛的营养物，从而使需要特定气氛的微生物直接在营养物质的体腔中生长。 在培养生长足够的时间之后，将其上的营养物质的载体从体腔中除去，并且例如在显微镜下检查，使用合适的染料染色生长以使后者容易可见。 用于营养物的载体可以采取具有小的突起的塑料片材的形式，其比由塑料片材承载的营养层更厚，并且其用于保持与塑料和营养物彼此分开的表面，否则其可能与每个 另一种是防止营养物质暴露于预定的气氛。 该制品优选地包装在其内部具有足够的水分以防止营养物干燥的容器中，容器的内部被用于容器的片材从外部大气封闭。
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86.发明授权Circulating device for loose materials, especially aerobic decomposing organic waste materials 失效用于松散材料的循环装置，特别是无机分解有机废物
公开(公告)号：US3642255A
公开(公告)日：1972-02-15
申请号：US3642255D
申请日：1970-05-27
申请人： GUJER HANS
发明人： GUJER HANS
CPC分类号： E02F3/10 , B65G65/06 , C05F17/0235 , Y02P20/145 , Y02W30/43
摘要： A circulating device for loose materials, especially for aerobic decomposing organic waste materials comprising a freely suspended, revolving, endless heavy link or jointed mat or conveyor structure which is guided over two upper deflecting devices arranged in spaced relationship from one another at an undercarriage, at least one of such deflecting devices being driven. The link mat is equipped at its outside surface or face with entrainment devices for the loose material, such entrainment devices being divided over its surface. The direction of rotation or revolving of the link mat occurring from the top towards the bottom at the side situated in the direction of advance of the circulation device.
摘要翻译： 一种用于松散材料的循环装置，特别是用于好氧分解有机废料的循环装置，包括一个自由悬挂的，旋转的，环形的重连杆或连接的垫或输送机结构，该两个上部偏转装置在起落架上以彼此间隔的关系布置， 至少一个这样的偏转装置被驱动。 连接垫在其外表面或面上装配有用于松散材料的夹带装置，这种夹带装置在其表面上分开。 从位于循环装置前进方向的一侧的顶部向底部发生的连杆垫的旋转或旋转的方向。
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87.发明授权Device and process for the study of the enzymatic characteristics of living cells 失效生活细胞的酶学特性研究的设备和方法
公开(公告)号：US3367841A
公开(公告)日：1968-02-06
申请号：US33949264
申请日：1964-01-22
申请人： PROMOVEO
发明人： BUISSIERE JEAN , LACROIX PIERRE
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公开(公告)号：US20240052282A1
公开(公告)日：2024-02-15
申请号：US17754706
申请日：2020-10-08
IPC分类号： C12M1/16 , C12M1/36 , C12M1/00 , C12M1/12 , A23L19/00 , A23L29/00 , B65B3/00 , B65B43/52 , B65B55/02 , B65B69/00 , B65B57/00
CPC分类号： C12M21/16 , C12M41/48 , C12M29/04 , C12M37/00 , A23L19/01 , A23L29/065 , B65B3/00 , B65B43/52 , B65B55/02 , B65B69/00 , B65B57/00
摘要： The invention relates to a process for large scale solid-state fermentation. The process comprises providing a substrate to be cultured (S1) made of plant material and/or animal material, filling vessels (S2) with the substrate using an automated filling system, sterilizing (S4) the vessels, inoculating (S5) the substrate with a microbial inoculant adapted to cause fermentation of the cultured substrate, storing (S6) the vessels in a closed state in controlled climate conditions for solid state fermentation of the cultured substrate, and harvesting (S7) the content of the vessels. Each vessel has an inner volume of 50 L or less and a smallest dimension less than or equal to 40 cm. This process is particularly adapted, with additional steps, for the production of fermented flour. In this process, upscaling is obtained by providing a high number of small bioreactors and by automation, instead of increasing the size of the reactors as generally done in the field of bioprocessing. The invention also relates to a corresponding production process.
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公开(公告)号：US20240034975A1
公开(公告)日：2024-02-01
申请号：US18017079
申请日：2021-07-08
申请人： NBTECH AB
发明人： Nathalie BEREZINA
摘要： A system (100, 200, 400, 700) and method for growing biomass, wherein the system comprises at least one growing unit (110, 210, 410, 710) having a respective controllable environment and comprising a first area (120, 220, 420, 720) and a second area (130, 230, 430, 730). The system further comprises at least one plate-grid device (140, 240, 441, 442, 443, 500, 740) arranged within said at least one growing unit in turn comprising at least one plate (225, 325, 510) elevated from at least one grid (226, 326, 530, 631, 632, 633, 634) wherein the at least one plate and the at least one grid are independently movable from one another between the first area and the second area. Additionally, the system comprises a decontamination device (160, 260, 461, 462) arranged within the second area and configured to decontaminate, in the second area, one or more of the at least one plate and/or at least one grid of the at least one plate-grid device. Furthermore, the at least one plate and at least one grid of each at least one plate-grid device are movable independently from the at least one plate and at least one grid of the other plate grid devices between a loading position (171, 471, 771), at which the at least one plate is free to receive a growth medium for growing biomass, and a discharging position (172, 472, 772), within the first area. The system further comprises at least one transporting mechanism (150, 250, 450, 750) configured to transport grown biomass discharged from the at least one plate-grid device at the discharging position, to a third area arranged outside of the at least one growing unit.
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公开(公告)号：US20230056666A1
公开(公告)日：2023-02-23
申请号：US17836922
申请日：2022-06-09
申请人： Ecovative Design LLC
摘要： The invention describes a methodology for production of a secondary extra-particle fungal matrix for application as a mycological material, manufactured via a Type II actively aerated static packed-bed bioreactor. A pre-conditioned air stream is passed through a substrate of discrete elements inoculated with a filamentous fungus to form an isotropic inter-particle hyphal matrix between the discrete elements. Continued feeding of the air through the substrate of discrete elements and isotropic inter-particle hyphal matrixes develops an extra-particle hyphal matrix that extends from an isotropic inter-particle hyphal matrix in the direction of airflow into a void space within the vessel.
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