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    METHODS, COMPOSITIONS, KITS, AND USES FOR ANALYSIS OF NUCLEIC ACIDS COMPRISING REPEATING A/T-RICH SEGMENTS
    1.
    发明申请
    METHODS, COMPOSITIONS, KITS, AND USES FOR ANALYSIS OF NUCLEIC ACIDS COMPRISING REPEATING A/T-RICH SEGMENTS 有权

    公开(公告)号：US20220136044A1

    公开(公告)日：2022-05-05

    申请号：US17456204

    申请日：2021-11-23

    申请人： Asuragen, Inc.

    发明人： Gary J. Latham ,  Sachin Sah

    IPC分类号： C12Q1/6844

    摘要： Described herein are methods, compositions, kits, and uses thereof for analysis of nucleic acid segments comprising a repeating A/T-rich segment, wherein the repeating A/T-rich segment is: (i) a homopolymeric segment comprising at least 10 A residues, at least 10 T residues, or at least 10 U residues, wherein the at least 10 A, T, or U residues are consecutive or interrupted once by one to three other nucleotides; or (ii) a segment comprising (TnA)m, (ATn)m, (TAn)m, or (AnT)m, wherein n is 2 or greater and m is such that the length of the repeating A/T-rich segment is 10 or more residues.

    PCR METHODS FOR CHARACTERIZING THE 5' UNTRANSLATED REGION OF THE FMR1 AND FMR2 GENES
    2.
    发明申请
    PCR METHODS FOR CHARACTERIZING THE 5' UNTRANSLATED REGION OF THE FMR1 AND FMR2 GENES 审中-公开
    用于表征FMR1和FMR2基因的5'未翻译区的PCR方法

    公开(公告)号：US20140248625A1

    公开(公告)日：2014-09-04

    申请号：US14191128

    申请日：2014-02-26

    申请人： Asuragen, Inc.

    发明人： Gary J. LATHAM ,  Liangjing CHEN ,  Sachin SAH

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6876 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2565/125 ,  C12Q2531/113 ,  C12Q2525/151

    摘要： This disclosure relates to methods of determining the presence and position of AGG or interruptor elements within a trinucleotide (for example, CGG) repeat region, and to methods of determining the number of repeats present in this region, by amplifying a set of products with a set of primers of which at least one comprises a portion of the CGG repeat region, and resolving the products to produce a representation of product size and abundance.

    摘要翻译： 本公开内容涉及确定三核苷酸（例如，CGG）重复区域内的AGG或干扰子元件的存在和位置的方法以及确定存在于该区域中的重复次数的方法，通过用 一组引物，其至少一个包含CGG重复区域的一部分，并且解析产物以产生产物尺寸和丰度的表示。

    COMPREHENSIVE FMR1 GENOTYPING
    3.
    发明申请
    COMPREHENSIVE FMR1 GENOTYPING 有权
    综合FMR1基因分析

    公开(公告)号：US20140024035A1

    公开(公告)日：2014-01-23

    申请号：US13946544

    申请日：2013-07-19

    申请人： Ashish Choudhary ,  Gary Latham ,  Sachin Sah

    发明人： Ashish Choudhary ,  Gary Latham ,  Sachin Sah

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6844 ,  G06F19/18

    摘要： Disclosed herein are methods for the automated reconstruction of a genotype of a gene, fragment, or genomic region using exhaustive enumeration. The methods can be used to reconstruct the genotype of any GC-rich sequence, such as the CGG repeat region in the 5′ UTR of FMR1 or the CCG repeat region in the 5′ UTR of FMR2. Also disclosed is an apparatus for use in conducting automated genotype reconstruction, as well as methods of diagnosis and treatment using exhaustive enumeration methods to reconstruct and identify genotypes associated with a disease or disorder.

    摘要翻译： 本文公开了使用详尽枚举来自动重建基因，片段或基因组区域的基因型的方法。 该方法可用于重建任何富含GC的序列的基因型，如FMR1的5'UTR中的CGG重复区或FMR2的5'UTR中的CCG重复区。 还公开了一种用于进行自动基因型重建的装置，以及使用穷举枚举方法来重建和鉴定与疾病或病症相关的基因型的诊断和治疗方法。

    Compositions and Methods for the Detection of Small RNA
    7.
    发明申请
    Compositions and Methods for the Detection of Small RNA 审中-公开
    小RNA检测的组成和方法

    公开(公告)号：US20080131878A1

    公开(公告)日：2008-06-05

    申请号：US11567082

    申请日：2006-12-05

    申请人： Gary J. Latham ,  Jon Kemppainen

    发明人： Gary J. Latham ,  Jon Kemppainen

    IPC分类号： C12Q1/68 ,  C07H21/02

    CPC分类号： C12Q1/6858 ,  C12Q2525/207 ,  C12Q2525/161 ,  C12Q2525/155

    摘要： Embodiments of the invention include methods of detecting one or more RNA by reverse transcribing one or more RNA target using one or more reverse transcription primer comprising in a 5′ to 3′ direction (i) a primer segment, (ii) a probe segment distinct from the primer segment, and (iii) a 3′ target specific segment that anneals to a RNA target; amplifying one or more RNA from the reverse transcription reaction using a first amplification primer that anneals to the 3′ end of a reverse transcribed RNA target and a second primer that anneals to a sequence complementary to the primer segment; and detecting amplification of a target nucleic acid.

    摘要翻译： 本发明的实施方案包括通过使用包含在5'至3'方向（i）引物区段的一个或多个逆转录引物逆转录一个或多个RNA靶标来检测一种或多种RNA的方法，（ii）探针片段不同 来自引物段，和（iii）与RNA靶标退火的3'靶标特异性区段; 使用与逆转录RNA靶的3'末端退火的第一扩增引物和与引物区段互补的序列退火的第二引物来从逆转录反应扩增一个或多个RNA; 并检测靶核酸的扩增。