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公开(公告)号：US20140141479A1
公开(公告)日：2014-05-22
申请号：US14163159
申请日：2014-01-24
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N9/1205 , Y02E50/10 , Y02P20/52
摘要： The invention relates to a recombinant host cell having (a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity; (b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity; and optionally (c) a modification in an endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase activity. Additionally, the invention relates to methods of making and using such recombinant host cells including, for example, methods of increasing glucose consumption, methods of improving redox balance, and/or methods of increasing the production of a product of a pyruvate-utilizing pathway.
摘要翻译： 本发明涉及重组宿主细胞，其具有（a）编码具有双重作用己糖激酶活性的多肽的内源多核苷酸的修饰; （b）编码具有己糖激酶活性的多肽的异源多核苷酸; 和任选地（c）编码具有丙酮酸脱羧酶活性的多肽的内源多核苷酸的修饰。 此外，本发明涉及制备和使用这种重组宿主细胞的方法，包括例如增加葡萄糖消耗的方法，改善氧化还原平衡的方法和/或增加丙酮酸利用途径产物生产的方法。
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公开(公告)号：US20140057329A1
公开(公告)日：2014-02-27
申请号：US13838869
申请日：2013-03-15
发明人： Youngen LI , Der-Ing Liao , Mark J. Nelson , Daniel P. Okeefe
IPC分类号： C12N9/04
CPC分类号： C12N9/0006 , C12N15/102 , C12N15/1058 , C12N15/70 , C12N15/81 , C12N2320/13 , C12P7/16 , C12P7/42 , C12Q1/26 , C12Y101/01086 , Y02E50/10 , Y02P20/52
摘要： Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
摘要翻译： 提供NADPH特异性酮酸酸还原异构酶进化以获得NADH特异性的方法。 描述了从已经进行协同因子切换以利用NADH的假单胞菌分离的特异性突变型酮酸酸还原异构酶。
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公开(公告)号：US20140030782A1
公开(公告)日：2014-01-30
申请号：US13840649
申请日：2013-03-15
发明人： Larry Cameron Anthony , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica Mcelvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
摘要： Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
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公开(公告)号：US20130183731A1
公开(公告)日：2013-07-18
申请号：US13646097
申请日：2012-10-05
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/0016 , C12N9/1022 , C12N9/1096 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/746 , C12N15/75 , C12N15/81 , C12Y101/01001 , C12Y101/01002 , C12Y101/01086 , C12Y102/00 , C12Y102/01003 , C12Y102/04004 , C12Y202/01006 , C12Y401/01072 , C12Y402/01009 , Y02E50/10 , Y02E50/343 , Y02P20/52
摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
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5.发明申请公开(公告)号：US20130164795A1
公开(公告)日：2013-06-27
申请号：US13524990
申请日：2012-06-15
CPC分类号： C12P7/6409 , A23K10/38 , A23K50/10 , C12P7/16 , C12P7/649 , Y02E50/10 , Y02E50/13 , Y02E50/17 , Y02P60/873
摘要： The present invention includes methods of generating co-products for animal feed and compositions useful as co-products for animal feed derived from biofuel production processes. More specifically, the invention includes co-products for animal feed from at least one process feedstream, such as fatty acids from oil hydrolysis, lipids from evaporation of thin stillage, syrup, distillers grains, distillers grains and solubles, solids from a mash before fermentation, and solids from a whole stillage after fermentation.
摘要翻译： 本发明包括产生用于动物饲料的副产物的方法和用作来自生物燃料生产方法的动物饲料的副产物的组合物。 更具体地，本发明包括用于来自至少一种工艺原料流的动物饲料的共同产品，例如来自油水解的脂肪酸，来自蒸馏酒精，糖浆，酒糟，酒糟和可溶物的脂质，发酵前的醪液中的固体 ，和发酵后的整个酒糟的固体。
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公开(公告)号：US08426174B2
公开(公告)日：2013-04-23
申请号：US12110510
申请日：2008-04-28
申请人： Michael G. Bramucci , Dennis Flint , Edward S. Miller, Jr. , Vasantha Nagarajan , Natalia Sedkova , Manjari Singh , Tina K. Van Dyk
发明人： Michael G. Bramucci , Dennis Flint , Edward S. Miller, Jr. , Vasantha Nagarajan , Natalia Sedkova , Manjari Singh , Tina K. Van Dyk
IPC分类号： C12P7/16
摘要： A method for the production of 2-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.
摘要翻译： 公开了通过使用微生物生产宿主发酵生产2-丁醇的方法。 该方法在发酵过程中使用温度降低，导致生产主体对丁醇产物的更强的耐受性。
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7.发明授权Recovery of butanol from a mixture of butanol, water, and an organic extractant 有权从丁醇，水和有机萃取剂的混合物中回收丁醇公开(公告)号：US08373009B2
公开(公告)日：2013-02-12
申请号：US12949154
申请日：2010-11-18
摘要： A process for recovering butanol from a mixture comprising a water-immiscible organic extractant, water, butanol, and optionally a non-condensable gas, is provided. The butanol is selected from 1-butanol, 2-butanol, isobutanol, and mixtures thereof. The extractant comprises at least one solvent selected from the group consisting of C7 to C22 fatty alcohols, C7 to C22 fatty acids, esters of C7 to C22 fatty acids, C7 to C22 fatty aldehydes, and mixtures thereof.
摘要翻译： 提供了一种从包含与水不混溶的有机萃取剂，水，丁醇和任选的不可冷凝气体的混合物中回收丁醇的方法。 丁醇选自1-丁醇，2-丁醇，异丁醇及其混合物。 萃取剂包含至少一种选自C7至C22脂肪醇，C7至C22脂肪酸，C7至C22脂肪酸酯，C7至C22脂肪醛及其混合物的溶剂。
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8.发明授权Recovery of butanol from a mixture of butanol, water, and an organic extractant 有权从丁醇，水和有机萃取剂的混合物中回收丁醇公开(公告)号：US08373008B2
公开(公告)日：2013-02-12
申请号：US12948945
申请日：2010-11-18
摘要： A process for recovering butanol from a mixture comprising a water-immiscible organic extractant, water, butanol, and optionally a non-condensable gas, is provided. The butanol is selected from 1-butanol, 2-butanol, isobutanol, and mixtures thereof. An overhead stream from a first distillation column is condensed to recover a mixed condensate. An entrainer is added to at least one appropriate process stream or vessel such that the mixed condensate comprises sufficient entrainer to provide phase separation of the organic and the aqueous phases to provide for recovery of the butanol.
摘要翻译： 提供了一种从包含与水不混溶的有机萃取剂，水，丁醇和任选的不可冷凝气体的混合物中回收丁醇的方法。 丁醇选自1-丁醇，2-丁醇，异丁醇及其混合物。 将来自第一蒸馏塔的塔顶物流冷凝以回收混合的冷凝物。 将夹带剂加入到至少一个合适的工艺流或容器中，使得混合的冷凝物包含足够的夹带剂以提供有机相和水相的相分离以提供丁醇的回收。
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公开(公告)号：US08273558B2
公开(公告)日：2012-09-25
申请号：US12966333
申请日：2010-12-13
摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达异丁醇生物合成途径的重组细菌的发酵生长来生产丁醇，优选异丁醇。
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10.发明授权Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof 有权具有Fe-S簇蛋白的重组酵母宿主细胞及其使用方法公开(公告)号：US08241878B2
公开(公告)日：2012-08-14
申请号：US13296944
申请日：2011-11-15
申请人： Larry Cameron Anthony , Dennis Flint , Wonchul Suh , Rick W. Ye , Steven Cary Rothman , Jean-Francois Tomb
发明人： Larry Cameron Anthony , Dennis Flint , Wonchul Suh , Rick W. Ye , Steven Cary Rothman , Jean-Francois Tomb
IPC分类号： C12P7/58
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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