公开(公告)号：US08865443B2

公开(公告)日：2014-10-21

申请号：US13894894

申请日：2013-05-15

申请人： Butamax(TM) Advanced Biofuels LLC

发明人： Keith H. Burlew ,  Robert Dicosimo ,  Michael Charles Grady

IPC分类号： C12N9/16 ,  C12N1/00 ,  C07C29/86 ,  C11B13/00 ,  C12P7/16 ,  C12M1/00 ,  B01D21/26 ,  C11B1/02 ,  B01D3/00 ,  C12P7/64

CPC分类号： C12P7/16 ,  A23K10/38 ,  B01D3/002 ,  B01D11/0492 ,  B01D21/26 ,  B01D21/262 ,  C07C29/86 ,  C11B1/025 ,  C11B13/00 ,  C12F3/10 ,  C12M1/00 ,  C12M21/12 ,  C12M45/04 ,  C12M45/09 ,  C12N1/14 ,  C12N15/00 ,  C12P7/18 ,  C12P7/6418 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02W30/74

摘要： In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant. The fermentation product extractant can be contacted with a fermentation broth for in situ removal of a product alcohol.

摘要翻译： 在酒精发酵过程中，将来自生物质的油水解成可从发酵液中原位除去产物醇如丁醇的萃取剂。 油中的甘油酯可以催化（例如，酶促）水解成游离脂肪酸，其形成产物醇的分配系数大于产物醇的生物质油的分配系数的发酵产物萃取剂。 由酒精发酵工艺的原料得到的油可以通过使包含油的原料与一种或多种酶接触来水解，由此至少一部分油被水解成形成发酵产物萃取剂的游离脂肪酸，或者油可以是 在原料进料到发酵容器之前与原料分离，并且分离的油可以与酶接触以形成发酵产物萃取剂。 发酵产物萃取剂可与发酵液接触，用于原位除去产物醇。

公开(公告)号：US08735114B2

公开(公告)日：2014-05-27

申请号：US13646097

申请日：2012-10-05

申请人： Butamax (TM) Advanced Biofuels LLC

发明人： Gail K Donaldson ,  Andrew C Eliot ,  Dennis Flint ,  Lori Ann Maggio-Hall ,  Vasantha Nagarajan

IPC分类号： C12P21/06 ,  C12P7/04 ,  C12P7/16 ,  C12N9/00 ,  C12N1/20 ,  C12N1/00 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0016 ,  C12N9/1022 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74 ,  C12N15/746 ,  C12N15/75 ,  C12N15/81 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01086 ,  C12Y102/00 ,  C12Y102/01003 ,  C12Y102/04004 ,  C12Y202/01006 ,  C12Y401/01072 ,  C12Y402/01009 ,  Y02E50/10 ,  Y02E50/343 ,  Y02P20/52

摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

公开(公告)号：US20140024064A1

公开(公告)日：2014-01-23

申请号：US13836115

申请日：2013-03-15

申请人： BUTAMAX(TM) ADVANCED BIOFUELS LLC

发明人： Keith H. Burlew ,  James Timothy Cronin ,  Benjamin Fuchs ,  John W. Hallam ,  David J. Lowe ,  Brian Michael Roesch ,  Mathias E. Stolarski ,  Joseph J. Zaher

IPC分类号： C12P7/16

CPC分类号： C12P7/16 ,  A23K10/38 ,  B01D21/02 ,  B01D21/26 ,  C11B1/00 ,  C11B1/025 ,  C11B1/10 ,  C11C3/003 ,  C12M21/12 ,  C12P7/06 ,  C12P7/10 ,  C12P7/14 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P60/873

摘要： The present invention relates to processes and systems for the production of fermentative alcohols such as ethanol and butanol. The present invention also provides methods for separating feed stream components for improved biomass processing and productivity.

摘要翻译： 本发明涉及用于生产发酵醇如乙醇和丁醇的方法和系统。 本发明还提供了用于分离进料流组分以提高生物质处理和生产率的方法。

公开(公告)号：US20130217060A1

公开(公告)日：2013-08-22

申请号：US13862626

申请日：2013-04-15

申请人： Butamax(TM) Advanced Biofuels LLC

发明人： Michael G. Bramucci ,  Dennis Flint ,  Edward S. Miller, JR. ,  Vasantha Nagarajan ,  Natalia Sedkova ,  Manjari Singh ,  Tina K. Van Dyk

IPC分类号： C12N15/74 ,  C12N15/70

CPC分类号： C12N15/74 ,  C12N15/70 ,  C12P7/16 ,  C12Y103/01038 ,  C12Y103/01044 ,  C12Y402/01017 ,  C12Y402/01055 ,  Y02E50/10

摘要： A method for the production of 1-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.

摘要翻译： 公开了通过使用微生物生产宿主发酵生产1-丁醇的方法。 该方法在发酵过程中使用温度降低，导致生产主体对丁醇产物的更强的耐受性。

公开(公告)号：US09238801B2

公开(公告)日：2016-01-19

申请号：US13838869

申请日：2013-03-15

申请人： BUTAMAX(TM) ADVANCED BIOFUELS LLC

发明人： Yougen Li ,  Der-Ing Liao ,  Mark J. Nelson ,  Daniel P. Okeefe

IPC分类号： C12N9/02 ,  C12N15/00 ,  C12P21/06 ,  C12P21/04 ,  C07H21/04 ,  C12N9/04 ,  C12N15/70 ,  C12N15/10 ,  C12Q1/26 ,  C12P7/16 ,  C12N15/81 ,  C12P7/42

CPC分类号： C12N9/0006 ,  C12N15/102 ,  C12N15/1058 ,  C12N15/70 ,  C12N15/81 ,  C12N2320/13 ,  C12P7/16 ,  C12P7/42 ,  C12Q1/26 ,  C12Y101/01086 ,  Y02E50/10 ,  Y02P20/52

摘要： Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.

公开(公告)号：US20140030783A1

公开(公告)日：2014-01-30

申请号：US13840676

申请日：2013-03-15

申请人： BUTAMAX(TM) ADVANCED BIOFUELS LLC

发明人： LARRY CAMERON ANTHONY ,  HONGXIAN HE ,  LIXUAN LISA HUANG ,  DANIEL P. OKEEFE ,  ARTHUR LEO KRUCKEBERG ,  YOUGEN LI ,  LORI ANN MAGGIO-HALL ,  JESSICA MCELVAIN ,  MARK J. NELSON ,  RANJAN PATNAIK ,  STEVEN CARY ROTHMAN

IPC分类号： C12P7/16

CPC分类号： C12P7/16 ,  C12N1/38 ,  C12N9/0006 ,  C12N15/81 ,  C12N15/815 ,  C12P7/42 ,  Y02E50/10 ,  Y02P20/52

摘要： Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.

摘要翻译： 本文提供重组酵母宿主细胞及其用于生产异丁醇的方法。 提供的酵母宿主细胞包含异丁醇生物合成途径和降低或消除的醛脱氢酶活性，降低或消除的乙酰乳酸还原酶活性中的至少一种; 或编码具有酮酸酸还原异构酶活性的多肽的异源多核苷酸。

公开(公告)号：US20140030776A1

公开(公告)日：2014-01-30

申请号：US13838508

申请日：2013-03-15

申请人： Butamax(TM) Advanced Biofuels LLC

发明人： Dennis FLINT ,  Steven Cary Rothman ,  Wonchul Suh ,  Jean-Francois Tomb ,  Rick W. Ye

IPC分类号： G06F19/22 ,  C12P7/16 ,  C12P7/40 ,  C12N9/88

CPC分类号： G06F19/22 ,  C12N9/88 ,  C12P7/16 ,  C12P7/40 ,  C12P13/06 ,  C12P13/08 ,  C12Y402/01009 ,  Y02E50/10

摘要： A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.

公开(公告)号：US20130344551A1

公开(公告)日：2013-12-26

申请号：US13838899

申请日：2013-03-15

申请人： BUTAMAX(TM) ADVANCED BIOFUELS LLC

发明人： Youngen LI ,  Der-Ing Liao ,  Mark J. Nelson ,  Daniel P. Okeefe

IPC分类号： C12P7/42 ,  C12P7/16 ,  C12N15/81

CPC分类号： C12N9/0006 ,  C12N15/102 ,  C12N15/1058 ,  C12N15/70 ,  C12N15/81 ,  C12N2320/13 ,  C12P7/16 ,  C12P7/42 ,  C12Q1/26 ,  C12Y101/01086 ,  Y02E50/10 ,  Y02P20/52

摘要： Methods for the evolution of NADPH specific ketol-add reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.

摘要翻译： 提供NADPH特异性酮加还原异构酶进化以获得NADH特异性的方法。 描述了从已经进行协同因子切换以利用NADH的假单胞菌分离的特异性突变型酮酸酸还原异构酶。

公开(公告)号：US09512435B2

公开(公告)日：2016-12-06

申请号：US13837921

申请日：2013-03-15

申请人： BUTAMAX(TM) ADVANCED BIOFUELS LLC

发明人： Dennis Flint ,  Brian James Paul ,  Rick W. Ye

IPC分类号： C12N15/81 ,  C12N9/88 ,  C07K14/395 ,  C12P7/16 ,  C12P7/40 ,  C12P13/02 ,  C12P13/04 ,  C12Q1/527 ,  G01N33/573

CPC分类号： C12P7/16 ,  C07K14/395 ,  C12N9/88 ,  C12N15/81 ,  C12P7/40 ,  C12P13/02 ,  C12P13/04 ,  C12Q1/527 ,  C12Y402/01009 ,  G01N33/573 ,  Y02E50/10 ,  Y02P20/52

摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.

公开(公告)号：US08889385B2

公开(公告)日：2014-11-18

申请号：US13838949

申请日：2013-03-15

申请人： Butamax(TM) Advanced Biofuels LLC

发明人： Gail K. Donaldson ,  Andrew C. Eliot ,  Dennis Flint ,  Lori Ann Maggio-Hall ,  Vasantha Nagarajan

IPC分类号： C12P21/06 ,  C12P7/04 ,  C12P7/16 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C12N15/52 ,  C12N15/75 ,  C12N15/74 ,  C12N9/02 ,  C12N9/10 ,  C12N15/81 ,  C12N9/88 ,  C12N9/06 ,  C12N9/04 ,  C12N15/70

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0016 ,  C12N9/1022 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74 ,  C12N15/746 ,  C12N15/75 ,  C12N15/81 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01086 ,  C12Y102/00 ,  C12Y102/01003 ,  C12Y102/04004 ,  C12Y202/01006 ,  C12Y401/01072 ,  C12Y402/01009 ,  Y02E50/10 ,  Y02E50/343 ,  Y02P20/52

摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达异丁醇生物合成途径的重组细菌的发酵生长来制备丁醇，优选异丁醇。