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    Insulin-like growth factor binding protein variants
    1.
    发明申请
    Insulin-like growth factor binding protein variants 审中-公开
    胰岛素样生长因子结合蛋白变体

    公开(公告)号：US20020072589A1

    公开(公告)日：2002-06-13

    申请号：US09986944

    申请日：2001-11-13

    申请人： Celtrix Pharmaceuticals, Inc.

    发明人： Desmond Mascarenhas ,  David Passmore ,  Stephen Danko

    IPC分类号： C07K016/22

    CPC分类号： C07K14/4743 ,  C07K2319/00

    摘要： The invention relates to variants of native insulin-like growth factor binding protein 3 (IGFBP-3). Variant IGFBP-3s are disclosed which are modified to be resistant to hydrolysis. Also disclosed are variant IGFBP-3s where the nuclear localization signal (NLS) in native IGFBP-3 is altered. Additionally, amino-terminally extended IGFBP-3s are disclosed which include a variety of N-terminal extensions, including peptide and nucleotide binding domains, specific binding members such as ligand binding domains from receptors or antigen binding domains from immunoglobins, and peptide and protein hormones and growth factors. N-terminally extended IGFBP-3s may comprise hydrolysis-resistant or NLS variant IGFBP-3s.

    摘要翻译： 本发明涉及天然胰岛素样生长因子结合蛋白3（IGFBP-3）的变体。 公开了修饰为耐水解的变体IGFBP-3。 还公开了其中天然IGFBP-3中的核定位信号（NLS）改变的变体IGFBP-3。 另外，公开了氨基末端延长的IGFBP-3，其包括多种N-末端延伸，包括肽和核苷酸结合结构域，特异性结合成员，例如来自免疫球蛋白的受体或抗原结合结构域的配体结合结构域，以及肽和蛋白激素 和生长因子。 N末端延长的IGFBP-3可以包含耐水解抗体或NLS变体IGFBP-3。

    Method for treating ophthalmic disorders with IGFBP
    2.
    发明授权
    Method for treating ophthalmic disorders with IGFBP 失效
    用IGFBP治疗眼部疾病的方法

    公开(公告)号：US6124259A

    公开(公告)日：2000-09-26

    申请号：US80366

    申请日：1998-02-24

    申请人： Michael J. Delmage ,  Andreas Sommer

    发明人： Michael J. Delmage ,  Andreas Sommer

    IPC分类号： A61K38/17 ,  A61K38/27 ,  A61K38/28

    CPC分类号： A61K38/1709

    摘要： This is a method for treating ophthalmic disorders associated with an excess of IGF-I or IGF-II. The method comprises administering individuals with an IGF excess insulin-like growth factor binding protein (IGFBP). The preferred form is IGFBP-3.

    摘要翻译： 这是一种治疗与过量IGF-I或IGF-II相关的眼科疾病的方法。 该方法包括给予个体具有IGF过量胰岛素样生长因子结合蛋白（IGFBP）。 优选的形式是IGFBP-3。

    Methods for treating diabetes
    3.
    发明授权
    Methods for treating diabetes 有权
    治疗糖尿病的方法

    公开(公告)号：US06040292A

    公开(公告)日：2000-03-21

    申请号：US326189

    申请日：1999-06-04

    申请人： Andreas Sommer

    发明人： Andreas Sommer

    IPC分类号： A61K38/00 ,  A61K38/22 ,  A61K38/30 ,  A61P3/10 ,  A61P9/10 ,  A61P13/12 ,  A61P25/00

    CPC分类号： A61K38/30

    摘要： The invention provides new methods for the treatment of diabetes mellitus, including type I, type II, and insulin resistant diabetes (both type I and type II). The methods of the invention employ administration of rhIGF-I/IGFBP-3 complex to a subject suffering from the symptoms of diabetes mellitus. Administration of rhIGF-I/IGFBP-3 to a subject suffering from the symptoms of diabetes mellitus results in amelioration or stabilization of the symptoms of diabetes.

    摘要翻译： 本发明提供了治疗糖尿病的新方法，包括I型，II型和胰岛素抵抗型糖尿病（I型和II型）。 本发明的方法使用向患有糖尿病症状的受试者施用rhIGF-1 / IGFBP-3复合物。 向患有糖尿病症状的受试者施用rhIGF-I / IGFBP-3导致糖尿病症状的改善或稳定。

    Method and composition for systemic treatment of tissue injury
    6.
    发明授权
    Method and composition for systemic treatment of tissue injury 失效
    全身治疗组织损伤的方法和组成

    公开(公告)号：US5407913A

    公开(公告)日：1995-04-18

    申请号：US984936

    申请日：1992-12-03

    申请人： Andreas Sommer ,  Christopher A. Maack

    发明人： Andreas Sommer ,  Christopher A. Maack

    IPC分类号： A61K38/30 ,  A61K37/36 ,  A61K31/56 ,  A61K33/16

    CPC分类号： A61K38/30

    摘要： This is a method for enhancing systemic tissue repair in patients with burns, trauma and peptic ulcers, as well as patients about to undergo, is undergoing or just having undergone surgery. The method comprises systemically administering to such individuals a therapeutic composition comprising IGF and IGFBP. The preferred form is IGF-I/IGFBP-3.

    摘要翻译： 这是一种用于增强烧伤，创伤和消化性溃疡患者的全身组织修复的方法，以及即将进行或正在进行手术的患者。 所述方法包括向所述个体全身施用包含IGF和IGFBP的治疗组合物。 优选的形式是IGF-I / IGFBP-3。

    Human somatomed in carrier protein subunits and process for producing them; recombinant DNA molecules, hosts, processes and human somatomedin carrier protein-like polypeptides
    7.
    发明申请
    Human somatomed in carrier protein subunits and process for producing them; recombinant DNA molecules, hosts, processes and human somatomedin carrier protein-like polypeptides 审中-公开
    人类生长在载体蛋白亚基中的过程及其生产过程; 重组DNA分子，宿主，过程和人生长因子载体蛋白样多肽

    公开(公告)号：US20010034433A1

    公开(公告)日：2001-10-25

    申请号：US09865578

    申请日：2001-05-29

    申请人： Celtrix Pharmaceuticals, Inc.

    发明人： Emerald Martin Spencer ,  Carol Talkington-Verser

    IPC分类号： C07H021/04 ,  C12P021/02 ,  A61K038/17 ,  C07K014/705

    CPC分类号： C07K14/4743 ,  A61K38/00 ,  Y02A50/473

    摘要： This invention relates to polypeptides that are human somatomedin carrier protein subunits and to processes for producing them. The carrier protein subunits bind to human somatomedin-like polypeptides, also known as insulin-like growth factors. The process involves preparation from a human serum fraction, Cohn IV-1, by a molecule of various chromatographic steps. This invention also relates to DNA molecules encoding human somatomedin carrier protein-like polypeptides, recombinant DNA molecules, hosts, processes for producing carrier protein-like polypeptides, human somatomedin carrier protein-like polypeptides produced using those molecules, hosts and processes. The invention relates to DNA molecules and their expression in appropriate hosts. The recombinant DNA molecules contain DNA molecules that code for polypeptides which have a biological activity of the human carrier protein or a human carrier protein subunit capable of binding somatomedins. The DNA molecules, recombinant DNA molecules, hosts, and processes of this invention may be used in the production of polypeptides useful in a variety of therapeutic, diagnostic, and other useful applications.

    摘要翻译： 本发明涉及作为人生长调节素载体蛋白亚基的多肽及其生产方法。 载体蛋白亚基结合人类生长调节素样多肽，也称胰岛素样生长因子。 该方法包括通过各种色谱步骤的分子由人血清级分Cohn IV-1制备。 本发明还涉及编码人生长调节素载体蛋白样多肽的DNA分子，重组DNA分子，宿主，产生载体蛋白样多肽的方法，使用这些分子产生的人类生长调节蛋白载体蛋白样多肽，宿主和过程。 本发明涉及DNA分子及其在适当宿主中的表达。 重组DNA分子包含编码具有人载体蛋白的生物活性的多肽的DNA分子或能够结合生长调节素的人载体蛋白亚基。 本发明的DNA分子，重组DNA分子，宿主和方法可用于制备可用于多种治疗，诊断和其它有用应用的多肽。

    Methods for increasing yields of recombinant proteins
    8.
    发明授权
    Methods for increasing yields of recombinant proteins 失效
    提高重组蛋白产量的方法

    公开(公告)号：US5914390A

    公开(公告)日：1999-06-22

    申请号：US855774

    申请日：1997-05-12

    申请人： Ranga Nathan ,  Henryk Cudny

    发明人： Ranga Nathan ,  Henryk Cudny

    IPC分类号： C12N15/09 ,  C07K1/113 ,  C07K14/47 ,  C12N1/06 ,  C12P21/02 ,  C07K1/00 ,  C07K14/00 ,  C12N15/00

    CPC分类号： C12N1/06 ,  C07K1/113 ,  C07K14/4743 ,  C12P21/02

    摘要： Methods are disclosed for increasing the yields of recombinant proteins. Addition of one or more protective agents, such as a sulfated compound (including heparin, heparin sulfate, sodium dodecyl sulfate, and the like), hyaluronic acid or deoxycholate, to recombinant protein production processes is disclosed. Addition of protective agents to the recombinant production process results in increased yields of recombinant proteins.

    摘要翻译： 公开了增加重组蛋白质产量的方法。 公开了一种或多种保护剂，例如硫酸化合物（包括肝素，硫酸肝素，十二烷基硫酸钠等），透明质酸或脱氧胆酸盐。 向重组生产过程中加入保护剂导致重组蛋白的产量提高。

    Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
    9.
    发明授权
    Expression of fusion polypeptides transported out of the cytoplasm without leader sequences 失效
    从细胞质中转移出的融合多肽的表达无前导序列

    公开(公告)号：US5914254A

    公开(公告)日：1999-06-22

    申请号：US854811

    申请日：1997-05-12

    申请人： Desmond Mascarenhas ,  Yang Zhang ,  Pamela S. Olson ,  David R. Olsen ,  Pedro A. Cohen ,  Kimyen B. Nguyen

    发明人： Desmond Mascarenhas ,  Yang Zhang ,  Pamela S. Olson ,  David R. Olsen ,  Pedro A. Cohen ,  Kimyen B. Nguyen

    IPC分类号： C07K14/47 ,  C07K19/00 ,  C12N15/62

    CPC分类号： C07K14/4743 ,  C07K2319/00 ,  C07K2319/32 ,  C07K2319/35 ,  C07K2319/75 ,  C07K2319/95

    摘要： The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted-translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof.

    摘要翻译： 本发明涉及不含前导序列的融合配偶体的用途，作为通过促进融合蛋白的表达增加重组多肽的溶解度和活性的手段，然后将其转运出细胞质。 本发明包括编码融合多肽的核酸，所述融合多肽包含成熟的白细胞介素-1样多肽或前导缺失的易位多肽和感兴趣的多肽; 以及包含这种核酸的宿主细胞，以及如此编码的融合蛋白。 本发明还包括使用此类核酸来产生重组融合多肽，目标成熟多肽及其纯化组合物的方法。