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    High flow technique for harvesting mammalian cells
    2.
    发明授权
    High flow technique for harvesting mammalian cells 失效
    用于收获哺乳动物细胞的高流量技术

    公开(公告)号：US5912163A

    公开(公告)日：1999-06-15

    申请号：US953541

    申请日：1997-10-20

    申请人： Kermit M. Borland ,  Barbara A. Chandler ,  Dianna Hunt Picton ,  Shawn P. Cain ,  Deborah Deane ,  Claudy J.P. Mullon

    发明人： Kermit M. Borland ,  Barbara A. Chandler ,  Dianna Hunt Picton ,  Shawn P. Cain ,  Deborah Deane ,  Claudy J.P. Mullon

    IPC分类号： A01N1/02 ,  C07G15/00

    CPC分类号： A01N1/0242 ,  A01N1/021 ,  A01N1/0247 ,  C12M45/09

    摘要： The invention is based in part on the observation that significant portions of porcine livers appear to remain intact after perfusion by standard methods, suggesting that the perfusion procedures employed do not result in complete enzymatic digestion. Recovery of cells is therefore substantially lower than would be possible if the organs were thoroughly digested. It is found that increased perfusion flow rate, occlusion of at least one major blood vessel leading out of the organ, increased enzymatic digestion time, and vigorous tissue dissociation techniques can be combined to afford a uniquely high yield of viable cells.

    摘要翻译： 本发明部分基于观察到，通过标准方法灌注后，猪肝脏的显着部分似乎保持完整，这表明所用的灌注程序不会导致完全的酶消化。 因此，如果器官被彻底消化，则细胞的恢复显着低于可能的。 发现增加的灌注流速，至少一个从器官出来的主要血管的阻塞增加了酶消化时间，并且可以组合有力的组织解离技术以提供独特的高产量的活细胞。

    Artificial pancreatic perfusion device
    5.
    发明授权
    Artificial pancreatic perfusion device 失效
    人工胰灌注装置

    公开(公告)号：US5741334A

    公开(公告)日：1998-04-21

    申请号：US732146

    申请日：1996-10-16

    申请人： Claudy Jean Paul Mullon ,  Karen E. Dunleavy

    发明人： Claudy Jean Paul Mullon ,  Karen E. Dunleavy

    IPC分类号： A61F2/02 ,  A61L27/38 ,  A61F2/04

    CPC分类号： A61L27/3804 ,  A61F2/022 ,  A61L27/3839

    摘要： An artifical pancreatic perfusion device comprising a hollow fiber having a porosity ranging from about 25 Kd to about 200 Kd. The hollow fiber has one end connected to a blood vessel for receiving blood and a second end connected to a blood vessel for returning said blood. Islets of Langerhans surround the hollow fiber. The hollow fiber and islets are surrounded by a housing comprising a semipermeable membrane having a pore size small enough to offer protection to the islets and host from immune reactive substances. Further disclosed is a method for providing and regulating insulin to insulin deficient individuals.

    摘要翻译： 一种人造胰腺灌注装置，其包括孔隙度范围为约25Kd至约200Kd的中空纤维。 中空纤维的一端连接到血管，用于接收血液，第二端连接到血管以返回血液。 朗格汉斯岛围绕中空纤维。 中空纤维和胰岛由壳体包围，该壳体包括具有足够小孔径的半透膜以提供对胰岛和宿主免疫反应性物质的保护。 进一步公开了一种用于向胰岛素缺乏个体提供和调节胰岛素的方法。

    Encapsulation device
    9.
    发明授权
    Encapsulation device 失效
    封装装置

    公开(公告)号：US6042909A

    公开(公告)日：2000-03-28

    申请号：US922910

    申请日：1997-09-03

    申请人： Karen E. Dunleavy ,  Timothy J. Perlman ,  Kerry A. Gagnon ,  Claudy J. P. Mullon

    发明人： Karen E. Dunleavy ,  Timothy J. Perlman ,  Kerry A. Gagnon ,  Claudy J. P. Mullon

    IPC分类号： A61L27/54 ,  A61L31/16 ,  A61K9/22 ,  A61M31/00 ,  C12M1/00 ,  C12N5/00

    CPC分类号： A61L31/16 ,  A61L27/54 ,  Y10S206/828 ,  Y10S424/07 ,  Y10T428/1352 ,  Y10T428/1379 ,  Y10T428/139 ,  Y10T428/24843 ,  Y10T428/2817 ,  Y10T428/2896

    摘要： The invention features an empty device for receiving a bioactive agent. The device includes a biocompatible and semi-permeable membrane that defines an enclosed space; the membrane also has at least one end that defines an opening for introducing the bioactive agent into the enclosed space. The device is configured to be placed in an animal.In one embodiment of the invention, the membrane has an inner surface and an outer surface, where the inner surface defines the inner surface, and includes a biocompatible adhesive in the general region of the opening to allow sealing of the opening after the introduction of the bioactive agent into the enclosed space.Another embodiment of the invention includes a biocompatible frame mounted in supporting relationship to the membrane and defining an opening for introducing the bioactive agent into the enclosed space. The frame has greater porosity than the membrane.

    摘要翻译： 本发明具有用于接收生物活性剂的空装置。 该装置包括限定封闭空间的生物相容性半透膜; 膜还具有限定用于将生物活性剂引入封闭空间的开口的至少一个端部。 该装置被配置成放置在动物中。 在本发明的一个实施例中，膜具有内表面和外表面，其中内表面限定内表面，并且在开口的一般区域中包括生物相容性粘合剂，以允许在引入开口之后密封开口 生物活性剂进入封闭空间。 本发明的另一个实施方案包括生物相容性框架，其以与膜的支撑关系安装并限定用于将生物活性剂引入封闭空间的开口。 框架具有比膜更大的孔隙率。