公开(公告)号：US20060078879A1

公开(公告)日：2006-04-13

申请号：US10503078

申请日：2003-02-04

申请人： Matthias Schwab ,  Elke Schaeffeler

发明人： Matthias Schwab ,  Elke Schaeffeler

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C12N9/10 ,  C07K16/40

CPC分类号： C12Q1/6883 ,  C12Q2600/156 ,  G01N2333/91011 ,  G01N2500/00

摘要： The present invention relates to a polymorphic TPMT polynucleotide. Moreover, the invention relates to genes or vectors comprising the polynucleotides of the invention and to a host cell genetically engineered with the polynucleotide or gene of the invention. Further, the invention relates to methods for producing molecular variant polypeptides or fragments thereof, methods for producing cells capable of expressing a molecular variant polypeptide and to a polypeptide or fragment thereof encoded by the polynucleotide or the gene of the invention or which is obtainable by the method or from the cells produced by the method of the invention. Furthermore, the invention relates to an antibody which binds specifically the polypeptide of the invention. Moreover, the invention relates to a transgenic non-human animal. The invention also relates to a solid support comprising one or a plurality of the above mentioned polynucleotides, genes, vectors, polypeptides, antibodies or host cells. Furthermore, methods of identifying a polymorphism, identifying and obtaining a pro-drug or drug or an inhibitor are also encompassed by the present invention. In addition, the invention relates to methods for producing of a pharmaceutical composition and to methods of diagnosing a disease. Further, the invention relates to a method of detection of the polynucleotide of the invention. Furthermore, comprised by the present invention are a diagnostic and a pharmaceutical composition. Even more, the invention relates to uses of the polynucleotides, genes, vectors, polypeptides or antibodies of the invention for the preparation of pharmaceutical or diagnostic compositions. Finally, the invention relates to a diagnostic kit.

摘要翻译： 本发明涉及多形态TPMT多核苷酸。 此外，本发明涉及包含本发明的多核苷酸的基因或载体，并涉及用本发明的多核苷酸或基因进行遗传工程改造的宿主细胞。 此外，本发明涉及用于产生分子变体多肽或其片段的方法，用于产生能够表达分子变体多肽的细胞的方法及其由本发明的多核苷酸或基因编码的多肽或其片段，或可由 方法或由本发明方法生产的细胞。 此外，本发明涉及特异性结合本发明多肽的抗体。 此外，本发明涉及转基因非人动物。 本发明还涉及包含一种或多种上述多核苷酸，基因，载体，多肽，抗体或宿主细胞的固体支持物。 此外，鉴定多态性，鉴定和获得前药或药物或抑制剂的方法也包括在本发明中。 此外，本发明涉及制备药物组合物的方法和诊断疾病的方法。 此外，本发明涉及本发明的多核苷酸的检测方法。 此外，本发明包括诊断和药物组合物。 更进一步地，本发明涉及本发明的多核苷酸，基因，载体，多肽或抗体在制备药物或诊断组合物中的用途。 最后，本发明涉及诊断试剂盒。

公开(公告)号：US20090311696A1

公开(公告)日：2009-12-17

申请号：US12404772

申请日：2009-03-16

申请人： Ulrich Zanger ,  Thomas Lang

发明人： Ulrich Zanger ,  Thomas Lang

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12N15/63 ,  G01N33/68

CPC分类号： C12N9/0038 ,  A01K2217/05 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/172 ,  G01N33/573

摘要： Described are general means and methods of diagnosing and treating the phenotypic spectrum as well as the overlapping clinical characteristics with several forms of inherited abnormal expression and/or function of the CYP2B6 genes. In particular, polynucleotides of molecular variant CYP2B6 genes which, for example, are associated with insufficient metabolization and/or sensitively of drugs, and vectors comprising such polynucleotides are provided. Furthermore, host cells comprising such polynucleotides or vectors and their use for the production of variant CYP2B6 proteins are described. In addition, variant CYP2B6 proteins and antibodies specifically recognizing such proteins as well as transgenic non-human animals comprising the above-described polynucleotide or vectors are provided. Described are also methods for identifying and obtaining inhibitors for therapy of disorders related to the malfunction of the CYP2B6 gene as well as methods of diagnosing the status of such disorders. Pharmaceutical and diagnostic compositions useful for diagnosing and treating various diseases with drugs that are substrates, inhibitors or modulators of the CYP2B6 gene product are described as well.

摘要翻译： 描述了诊断和治疗表型谱的一般手段和方法以及CYP2B6基因的几种形式的遗传异常表达和/或功能的重叠临床特征。 特别地，提供了例如与代谢不足和/或敏感性相关的分子变体CYP2B6基因的多核苷酸以及包含这种多核苷酸的载体。 此外，描述了包含这种多核苷酸或载体的宿主细胞及其用于产生变体CYP2B6蛋白的用途。 此外，提供变体CYP2B6蛋白质和特异性识别这些蛋白质的抗体以及包含上述多核苷酸或载体的转基因非人动物。 还描述了用于鉴定和获得用于治疗与CYP2B6基因功能紊乱相关的疾病的抑制剂的方法以及诊断此类疾病状态的方法。 也描述了可用于诊断和治疗各种疾病的药物和诊断组合物，其中药物是CYP2B6基因产物的底物，抑制剂或调节剂。

公开(公告)号：US20020160479A1

公开(公告)日：2002-10-31

申请号：US10007814

申请日：2001-11-09

申请人： Epidauros Biotechnologie AG

发明人： Leszek Wojnowski ,  Klaus Gellner ,  Regina Eiselt

IPC分类号： C12N009/02 ,  C07H021/04 ,  C12P021/02 ,  C12N005/06

CPC分类号： C12N9/0077 ,  A01K2217/05 ,  C40B40/00

摘要： The present invention relates to polynucleotides encoding the CYP3AX protein and variants thereof. Further, the present invention also provides vectors comprising said polynucleotides, in particular vectors, wherein polynucleotides of the present invention are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells. In addition, the present invention relates to proteins encoded by said polynucleotides and antibodies specifically recognizing such proteins. The present invention also concerns transgenic non-human animals comprising the above-described polynucleotide or vectors. Moreover, the present invention relates to methods for identifying and obtaining drug candidates and inhibitors for therapy of disorders related to the malfunction of the CYP3AX genes as well as to methods of diagnosing the status of such disorders. The present invention also relates to methods for the identification of molecular variants of the CYP3AX polynucleotide or protein. The present invention furthermore provides pharmaceutical and diagnostic compositions comprising the above-described polynucleotides, vectors, proteins, antibodies, drugs and inhibitors obtainable by the above-described method. Said compositions are particularly useful for diagnosing and treating various diseases with drugs that are substrates, inhibitors or modulators of CYP3AX genes or their product.

摘要翻译： 本发明涉及编码CYP3AX蛋白的多核苷酸及其变体。 此外，本发明还提供了包含所述多核苷酸，特别是载体的载体，其中本发明的多核苷酸可操作地连接到允许在原核和/或真核宿主细胞中表达的调节元件。 此外，本发明涉及由所述多核苷酸编码的蛋白质和特异性识别这些蛋白质的抗体。 本发明还涉及包含上述多核苷酸或载体的转基因非人动物。 此外，本发明涉及用于鉴定和获得药物候选物的方法和用于治疗与CYP3AX基因故障相关的疾病的抑制剂以及诊断这些病症状态的方法。 本发明还涉及用于鉴定CYP3AX多核苷酸或蛋白质的分子变体的方法。 本发明还提供包含上述多核苷酸，载体，蛋白质，抗体，药物和可通过上述方法获得的抑制剂的药物和诊断组合物。 所述组合物特别可用于利用作为CYP3AX基因的底物，抑制剂或调节剂或其产物的药物来诊断和治疗各种疾病。

公开(公告)号：US20090061452A1

公开(公告)日：2009-03-05

申请号：US12221130

申请日：2008-07-30

申请人： Reinhold Kerb ,  Hermann Koepsell ,  Ulrich Brinkmann

发明人： Reinhold Kerb ,  Hermann Koepsell ,  Ulrich Brinkmann

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C40B30/04

CPC分类号： C07K14/4702 ,  A01K2217/05 ,  A61K38/00 ,  C12Q1/6883

摘要： The present invention relates to a polymorphic OCT1 polynucleotide. Moreover, the invention relates to genes or vectors comprising the polynucleotides of the invention and to a host cell genetically engineered with the polynucleotide or gene of the invention. Further, the invention relates to methods for producing molecular variant polypeptides or fragments thereof, methods for producing cells capable of expressing a molecular variant polypeptide and to a polypeptide or fragment thereof encoded by the polynucleotide or the gene of the invention or which is obtainable by the method or from the cells produced by the method of the invention. Furthermore, the invention relates to an antibody which binds specifically the polypeptide of the invention. Moreover, the invention relates to a transgenic non-human animal. The invention also relates to a solid support comprising one or a plurality of the above mentioned polynucleotides, genes, vectors, polypeptides, antibodies or host cells. Furthermore, methods of identifying a polymorphism, identifying and obtaining a pro-drug or drug or an inhibitor are also encompassed by the present invention. In addition, the invention relates to methods for producing of a pharmaceutical composition and to methods of diagnosing a disease. Further, the invention relates to a method of detection of the polynucleotide of the invention. Furthermore, comprised by the present invention are a diagnostic and a pharmaceutical composition. Even more, the invention relates to uses of the polynucleotides, genes, vectors, polypeptides or antibodies of the invention. Finally, the invention relates to a diagnostic kit.

摘要翻译： 本发明涉及多晶型OCT1多核苷酸。 此外，本发明涉及包含本发明的多核苷酸的基因或载体，并涉及用本发明的多核苷酸或基因进行遗传工程改造的宿主细胞。 此外，本发明涉及用于产生分子变体多肽或其片段的方法，用于产生能够表达分子变体多肽的细胞的方法及其由本发明的多核苷酸或基因编码的多肽或其片段，或可由 方法或由本发明方法生产的细胞。 此外，本发明涉及特异性结合本发明多肽的抗体。 此外，本发明涉及转基因非人动物。 本发明还涉及包含一种或多种上述多核苷酸，基因，载体，多肽，抗体或宿主细胞的固体支持物。 此外，鉴定多态性，鉴定和获得前药或药物或抑制剂的方法也包括在本发明中。 此外，本发明涉及制备药物组合物的方法和诊断疾病的方法。 此外，本发明涉及本发明的多核苷酸的检测方法。 此外，本发明包括诊断和药物组合物。 更进一步地，本发明涉及本发明的多核苷酸，基因，载体，多肽或抗体的用途。 最后，本发明涉及诊断试剂盒。

公开(公告)号：US20090144841A1

公开(公告)日：2009-06-04

申请号：US11901238

申请日：2007-09-13

申请人： Ulrich Brinkmann ,  Sven Hoffmeyer ,  Esther Mornhinweg

发明人： Ulrich Brinkmann ,  Sven Hoffmeyer ,  Esther Mornhinweg

IPC分类号： A01K67/027 ,  C07H21/00 ,  C12N15/74 ,  C12N5/10 ,  C12Q1/68 ,  A61K31/7088 ,  A61P35/00 ,  G01N33/567 ,  C12P21/00 ,  C12N15/02 ,  C07K16/18

CPC分类号： C12Q1/6883 ,  C07K14/47 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/6893 ,  G01N2333/705 ,  G01N2500/04 ,  G01N2800/44

摘要： The present invention relates to a polymorphic MRP-1 polynucleotide. Moreover, the invention relates to genes or vectors comprising the polynucleotides of the invention and to a host cell genetically engineered with the polynucleotide or gene of the invention. Further, the invention relates to methods for producing molecular variant polypeptides or fragments thereof, methods for producing cells capable of expressing a molecular variant polypeptide and to a polypeptide or fragment thereof encoded by the polynucleotide or the gene of the invention or which is obtainable by the method or from the cells produced by the method of the invention. Furthermore, the invention relates to an antibody which binds specifically the polypeptide of the invention. Moreover, the invention relates to a transgenic non-human animal. The invention also relates to a solid support comprising one or a plurality of the above mentioned polynucleotides, genes, vectors, polypeptides, antibodies or host cells. Furthermore, methods of identifying a polymorphism, identifying and obtaining a pro-drug or drug or an inhibitor are also encompassed by the present invention. In addition, the invention relates to methods for producing of a pharmaceutical composition and to methods of diagnosing a disease. Further, the invention relates to a method of detection of the polynucleotide of the invention. Furthermore, comprised by the present invention are a diagnostic and a pharmaceutical composition. Even more, the invention relates to uses of the polynucleotides, genes, vectors, polypeptides or antibodies of the invention. Finally, the invention relates to a diagnostic kit.

摘要翻译： 本发明涉及多形性MRP-1多核苷酸。 此外，本发明涉及包含本发明的多核苷酸的基因或载体，并涉及用本发明的多核苷酸或基因进行遗传工程改造的宿主细胞。 此外，本发明涉及用于产生分子变体多肽或其片段的方法，用于产生能够表达分子变体多肽的细胞的方法及其由本发明的多核苷酸或基因编码的多肽或其片段，或可由 方法或由本发明方法生产的细胞。 此外，本发明涉及特异性结合本发明多肽的抗体。 此外，本发明涉及转基因非人动物。 本发明还涉及包含一种或多种上述多核苷酸，基因，载体，多肽，抗体或宿主细胞的固体支持物。 此外，鉴定多态性，鉴定和获得前药或药物或抑制剂的方法也包括在本发明中。 此外，本发明涉及制备药物组合物的方法和诊断疾病的方法。 此外，本发明涉及本发明的多核苷酸的检测方法。 此外，本发明包括诊断和药物组合物。 更进一步地，本发明涉及本发明的多核苷酸，基因，载体，多肽或抗体的用途。 最后，本发明涉及诊断试剂盒。

公开(公告)号：US20090018030A1

公开(公告)日：2009-01-15

申请号：US12221241

申请日：2008-07-30

申请人： Reinhold Kerb ,  Hermann Koepsell ,  Ulrich Brinkmann

发明人： Reinhold Kerb ,  Hermann Koepsell ,  Ulrich Brinkmann

IPC分类号： C40B30/04 ,  C12Q1/68 ,  G01N33/68 ,  G01N33/53

CPC分类号： C07K14/4702 ,  A01K2217/05 ,  A61K38/00 ,  C12Q1/6883

摘要： The present invention relates to a polymorphic OCT1 polynucleotide. Moreover, the invention relates to genes or vectors comprising the polynucleotides of the invention and to a host cell genetically engineered with the polynucleotide or gene of the invention. Further, the invention relates to methods for producing molecular variant polypeptides or fragments thereof, methods for producing cells capable of expressing a molecular variant polypeptide and to a polypeptide or fragment thereof encoded by the polynucleotide or the gene of the invention or which is obtainable by the method or from the cells produced by the method of the invention. Furthermore, the invention relates to an antibody which binds specifically the polypeptide of the invention. Moreover, the invention relates to a transgenic non-human animal. The invention also relates to a solid support comprising one or a plurality of the above mentioned polynucleotides, genes, vectors, polypeptides, antibodies or host cells. Furthermore, methods of identifying a polymorphism, identifying and obtaining a pro-drug or drug or an inhibitor are also encompassed by the present invention. In addition, the invention relates to methods for producing of a pharmaceutical composition and to methods of diagnosing a disease. Further, the invention relates to a method of detection of the polynucleotide of the invention. Furthermore, comprised by the present invention are a diagnostic and a pharmaceutical composition. Even more, the invention relates to uses of the polynucleotides, genes, vectors, polypeptides or antibodies of the invention. Finally, the invention relates to a diagnostic kit.

摘要翻译： 本发明涉及多晶型OCT1多核苷酸。 此外，本发明涉及包含本发明的多核苷酸的基因或载体，并涉及用本发明的多核苷酸或基因进行遗传工程改造的宿主细胞。 此外，本发明涉及用于产生分子变体多肽或其片段的方法，用于产生能够表达分子变体多肽的细胞的方法及其由本发明的多核苷酸或基因编码的多肽或其片段，或可由 方法或由本发明方法生产的细胞。 此外，本发明涉及特异性结合本发明多肽的抗体。 此外，本发明涉及转基因非人动物。 本发明还涉及包含一种或多种上述多核苷酸，基因，载体，多肽，抗体或宿主细胞的固体支持物。 此外，鉴定多态性，鉴定和获得前药或药物或抑制剂的方法也包括在本发明中。 此外，本发明涉及制备药物组合物的方法和诊断疾病的方法。 此外，本发明涉及本发明的多核苷酸的检测方法。 此外，本发明包括诊断和药物组合物。 更进一步地，本发明涉及本发明的多核苷酸，基因，载体，多肽或抗体的用途。 最后，本发明涉及诊断试剂盒。