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22 条记录 (耗时 0.014 秒)

    Strip electrode with screen printing
    1.
    发明授权
    Strip electrode with screen printing 失效
    带电极丝网印刷

    公开(公告)号：US5727548A

    公开(公告)日：1998-03-17

    申请号：US469205

    申请日：1995-06-06

    申请人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

    发明人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

    IPC分类号： A61B5/00 ,  C12Q1/00 ,  C12Q1/68 ,  G01N27/403 ,  G01N33/487 ,  G01N33/543

    CPC分类号： G01N33/5438 ,  A61B5/1486 ,  A61B5/6848 ,  C12Q1/001 ,  C12Q1/004 ,  C12Q1/6825 ,  G01N27/4035 ,  A61B2560/0418 ,  Y10T29/4914 ,  Y10T29/49153 ,  Y10T29/49169 ,  Y10T29/49224

    摘要： A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a single layer deposit of an enzyme capable of catalyzing a reaction involving the compound, a conductive material, and an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing, an admixture of an enzyme a conductive material, and a mediator as a single layer onto the substrate.

    摘要翻译： 检测液体混合物中的化合物的当前代表的传感器系统具有适于可释放地附接到信号读出电路的双电极条。 条带包括适于可释放地附接到读出电路的细长支撑件（优选是平坦的）; 第一导体和第二导体各自沿着支撑件延伸并且包括用于连接到电路的装置。 定位成接触液体混合物和第一导体的活性电极包括能够催化涉及化合物，导电材料和电子介体的反应的酶的单层沉积物，其能够在酶催化的 反应和第一个导体。 定位参考电极以接触混合物和第二导体。 该系统包括适于提供代表电流的电信号的电路。 双电极条例如通过丝网印刷，酶导电材料的混合物和作为单层的介体制成在基底上。

    Determination of amylase
    2.
    发明授权
    Determination of amylase 失效
    淀粉酶的测定

    公开(公告)号：US4912041A

    公开(公告)日：1990-03-27

    申请号：US247780

    申请日：1988-09-21

    申请人： Mark J. Batchelor ,  Stephen C. Williams ,  Mokina J. Green

    发明人： Mark J. Batchelor ,  Stephen C. Williams ,  Mokina J. Green

    IPC分类号： C12Q1/00 ,  G01N27/49

    CPC分类号： C12Q1/005 ,  Y10S435/81

    摘要： A method of assay for the presence of .alpha.-amylase in a sample employs a derivatized .alpha.-oligosaccharide which can be hydrolyzed by an enzyme mix including .alpha.-amylase to yield an electroactive compound, and at least one other enzyme which with .alpha.-amylase gives an enzyme mix capable of hydrolyzing the derivatized .alpha.-oligosaccharide. The sample is contacted with the derivatized .alpha.-oligosaccharide and with the other enzyme to permit hydrolysis of the derivatized .alpha.-oligosaccharide and to allow formation of the electroactive compound. An electrode is poised at a potential at which the derivatized .alpha.-oligosaccharide is not electochemically active, the potential being one at which the electroactive compound is electochemically active. Any current arising from electrochemical activity of the electroactive compound is detected.

    Electrochemical assay for nucleic acids and nucleic acid probes
    3.
    发明授权
    Electrochemical assay for nucleic acids and nucleic acid probes 失效
    核酸和核酸探针的电化学测定

    公开(公告)号：US4840893A

    公开(公告)日：1989-06-20

    申请号：US769629

    申请日：1985-10-15

    申请人： Hugh A. O. Hill ,  Susan I. Libor

    发明人： Hugh A. O. Hill ,  Susan I. Libor

    IPC分类号： C12Q1/00 ,  C12Q1/54 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/58

    CPC分类号： G01N33/581 ,  C12Q1/00 ,  C12Q1/004 ,  C12Q1/54 ,  C12Q1/6825 ,  G01N33/5438 ,  Y10S435/81 ,  Y10S435/817 ,  Y10S436/806 ,  Y10S436/904

    摘要： The present invention discloses an assay for nucleic acid which comprises the steps of;(a) providing a probe material comprising;(i) a sequence of nucleic acids complementary to a given target sequence and,(ii) a first ligand chemically linked thereto and capable of a specific binding reaction with an antiligand;(b) contacting the said probe material with an assay system comprising:(i) a suitable mediator, enzyme, substrate system capable of transferring charge to an electrode surface when the enzyme is catalytically active, and;(ii) a second ligand chemically linked to one of said mediator, enzyme or substrate, wherein the second ligand is capable of a competitive binding reaction with the antiligand, and;(iii) the said antiligand, Whereby the said first ligand competes with the said second ligand in a specific binding reaction with the antiligand, and;(c) contacting the above system with a solution suspected of containing the said target sequence whereby the binding of any of the said target sequence present to the probe affects the availability of the first ligand and therefore alters the rate of charge transfer to the electrode.

    摘要翻译： PCT No.PCT / GB84 / 00432 Sec。 371日期1985年10月15日第 102（e）日期1985年10月15日PCT提交1984年12月14日PCT公布。 出版物WO85 / 02627 1985年6月20日，本发明公开了一种核酸检测方法，包括以下步骤： （a）提供探针材料，包括： （i）与给定靶序列互补的核酸序列，和（ii）与其结合化学连接且能够与抗配体结合的特异性结合反应的第一配体; （b）使所述探针材料与测定系统接触，所述测定系统包括：（i）当酶是催化活性时能够将电荷转移到电极表面的合适的介体，酶，底物系统; （ii）与所述介体，酶或底物之一化学连接的第二配体，其中所述第二配体能够与所述反配体发生竞争性结合反应;和 （iii）所述抗配糖剂，其中所述第一配体与所述第二配体竞争与所述反配体的特异性结合反应; （c）将上述系统与疑似含有所述靶序列的溶液接触，由此存在的任何所述靶序列与探针的结合影响第一配体的可用性，并因此改变电荷向电极转移的速率。

    Strip electrode including screen printing of a single layer
    7.
    发明授权
    Strip electrode including screen printing of a single layer 失效
    带状电极，包括单层丝网印刷

    公开(公告)号：US5509410A

    公开(公告)日：1996-04-23

    申请号：US281237

    申请日：1994-07-27

    申请人： Hugh A. O. Hill ,  Irving J. Higgins ,  James M. McCann ,  Graham Davis

    发明人： Hugh A. O. Hill ,  Irving J. Higgins ,  James M. McCann ,  Graham Davis

    IPC分类号： A61B5/00 ,  C12Q1/00 ,  C12Q1/68 ,  G01N27/403 ,  G01N33/487 ,  G01N33/543

    CPC分类号： G01N33/5438 ,  A61B5/1486 ,  A61B5/6848 ,  C12Q1/001 ,  C12Q1/004 ,  C12Q1/6825 ,  G01N27/4035 ,  A61B2560/0418 ,  Y10T29/4914 ,  Y10T29/49153 ,  Y10T29/49169 ,  Y10T29/49224

    摘要： A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a deposit of an enzyme capable of catalyzing a reaction involving the compound and (preferably) an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing an admixture of enzyme, a conductive material, and a mediator as a single layer onto the substrate.

    摘要翻译： 检测液体混合物中的化合物的当前代表的传感器系统具有适于可释放地附接到信号读出电路的双电极条。 条带包括适于可释放地附接到读出电路的细长支撑件（优选是平坦的）; 第一导体和第二导体各自沿着支撑件延伸并且包括用于连接到电路的装置。 定位成接触液体混合物和第一导体的活性电极包括能够催化涉及化合物和（优选）电子介体的反应的酶的沉积物，其能够在酶催化的反应和第一个 导体。 定位参考电极以接触混合物和第二导体。 该系统包括适于提供代表电流的电信号的电路。 双电极条的制造，例如通过将酶，导电材料和介体的混合物作为单层丝网印刷到基底上。

    Mediators to oxidoreductase enzymes
    10.
    发明授权
    Mediators to oxidoreductase enzymes 失效
    介导氧化还原酶

    公开(公告)号：US5710011A

    公开(公告)日：1998-01-20

    申请号：US400133

    申请日：1995-03-06

    申请人： Nigel Forrow ,  Stephen Walters

    发明人： Nigel Forrow ,  Stephen Walters

    IPC分类号： C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/566

    CPC分类号： C12Q1/26 ,  C12Q1/004 ,  Y10S435/817 ,  Y10S436/801

    摘要： Half-sandwich complexes of transition metals having a single .pi.-ligand with delocalised .pi. bonding are of use in mediating electron transfer between an electrode and a redox protein, for example an oxidoreductase enzyme such as in an amperometric enzyme sensor. Suitable compounds include complexes of the general formula: (.pi.-ligand)M(not-.pi.-ligand).sub.n wherein the complex is charged or neutral, .pi.-ligand represents the single .pi.-bonded ligand, M represents the transition metal atom, n is the number of ligands which are not .pi.-ligands, and the n not-.pi.-ligands are the same or different, are univalent or multivalent, and serve to satisfy the valency of the transitional metal M.

    摘要翻译： 具有单一pi配体和具有离域pI键的过渡金属的半夹心复合物可用于介导电极和氧化还原蛋白之间的电子转移，例如氧化还原酶，例如电流型酶传感器。 合适的化合物包括通式（pi -ligand）M（不对称）n的络合物，其中络合物带电或中性，π-配体代表单个π键配体，M代表过渡金属原子，n 是不是π-配体的配体的数量，并且n个不同的配位相同或不同，是一价或多价的，并且用于满足过渡金属M的化合价。