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    METHODS FOR DISTINGUISHING PARTICLES IN A FLUID SAMPLE
    2.
    发明公开
    METHODS FOR DISTINGUISHING PARTICLES IN A FLUID SAMPLE 审中-公开

    公开(公告)号：US20230360413A1

    公开(公告)日：2023-11-09

    申请号：US17888936

    申请日：2022-08-16

    申请人： Optofluidics Inc.

    发明人： Bernardo Cordovez ,  Paul Dyer ,  Spencer Borg ,  Adam Ross

    IPC分类号： G06V20/69 ,  G01N15/06 ,  G01N1/30 ,  G06V10/56

    CPC分类号： G06V20/693 ,  G01N15/0625 ,  G01N1/30 ,  G06V10/56 ,  G06V20/698 ,  G01N2015/0065

    摘要： Methods for distinguishing particles in a fluid sample are disclosed. In one embodiment, the method includes acquiring a background SIMI image and a background brightfield image of a membrane filter while the membrane filter is free of a fluid sample, introducing a fluid sample onto the membrane filter, acquiring a SIMI image and a brightfield image of filtered particles resting on the membrane filter, distinguishing between the filtered particulates and the membrane filter based on the background SIMI image, generating a particle mask based on the SIMI image, and detecting beads via the particle mask. Methods for distinguishing particulates include distinguishing between viable and non-viable cell populations, distinguishing between cellular and non-cellular particulates, distinguishing between biological and non-biological particulates, distinguishing between first and second protein types, determining stability of monoclonal antibody drugs, identifying beads in a cell therapy, and detecting bacteria.

    VACUUM MANIFOLD FOR FILTRATION MICROSCOPY
    3.
    发明公开
    VACUUM MANIFOLD FOR FILTRATION MICROSCOPY 审中-公开

    公开(公告)号：US20230280253A1

    公开(公告)日：2023-09-07

    申请号：US18315911

    申请日：2023-05-11

    申请人： Optofluidics Inc.

    发明人： Thomas Castner ,  Robert Hart ,  Colby Ashcroft ,  Brian DiPaolo ,  Nathan Wall

    IPC分类号： G01N15/02 ,  G01N15/14 ,  G06T7/00

    CPC分类号： G01N15/0227 ,  G01N15/14 ,  G06T7/001 ,  G06T2207/10056 ,  G01N2015/1493

    摘要： A vacuum manifold for filtration microscopy includes a manifold top having multiple openings, and a capture membrane positioned above and spaced apart from the manifold top, where the capture membrane is configured to deflect into contact with a surface of the manifold top when a negative pressure is applied to the multiple openings. A method for filtration microscopy includes the steps of providing a vacuum manifold including a manifold top having a plurality of openings, and a capture membrane positioned above and spaced apart from the manifold top; applying sample drops to sample spots on the membrane, the sample spots positioned above the plurality of openings; applying a negative pressure to the openings such that the capture membrane contacts a surface of the manifold top; and optically imaging particulates on the capture membrane.

    METHODS FOR IDENTIFICATION OF PARTICLES IN A FLUID SAMPLE
    5.
    发明申请
    METHODS FOR IDENTIFICATION OF PARTICLES IN A FLUID SAMPLE 有权

    公开(公告)号：US20210285884A1

    公开(公告)日：2021-09-16

    申请号：US17331968

    申请日：2021-05-27

    申请人： Optofluidics Inc.

    发明人： Bernardo Cordovez ,  Colby Ashcroft ,  Brian DiPaolo ,  Gjergji Konica ,  Robert Hart ,  Alexey Aprelev

    IPC分类号： G01N21/64 ,  G01N1/30 ,  G01N33/68

    摘要： A method of distinguishing between proteinaceous and non-proteinaceous particulates in a fluid sample includes the steps of acquiring a brightfield background image of a membrane filter, introducing a fluid sample onto the membrane filter, acquiring a brightfield image of filtered particles resting on the membrane filter, generating a particle mask based on the brightfield background image and the brightfield image of filtered particles, introducing a fluorescent dye onto the membrane filter, detecting fluorescence on the particle mask, and distinguishing between proteinaceous and non-proteinaceous particulates based on the detected fluorescence. A method for detecting other types of particles, such as polysorbate particles, silicone oil or protein monomers is also disclosed.

    Vacuum manifold for filtration microscopy
    6.
    发明授权
    Vacuum manifold for filtration microscopy 有权

    公开(公告)号：US10890514B2

    公开(公告)日：2021-01-12

    申请号：US16394173

    申请日：2019-04-25

    申请人： Optofluidics Inc.

    发明人： Thomas Castner ,  Robert Hart ,  Colby Ashcroft ,  Brian DiPaolo ,  Nathan Wall

    IPC分类号： G01J3/46 ,  G01N15/02 ,  G01N15/14 ,  G06T7/00

    摘要： A vacuum manifold for filtration microscopy includes a manifold top having multiple openings, and a capture membrane positioned above and spaced apart from the manifold top, where the capture membrane is configured to deflect into contact with a surface of the manifold top when a negative pressure is applied to the multiple openings. A method for filtration microscopy includes the steps of providing a vacuum manifold including a manifold top having a plurality of openings, and a capture membrane positioned above and spaced apart from the manifold top; applying sample drops to sample spots on the membrane, the sample spots positioned above the plurality of openings; applying a negative pressure to the openings such that the capture membrane contacts a surface of the manifold top; and optically imaging particulates on the capture membrane.

    Vacuum manifold for filtration microscopy
    7.
    发明授权
    Vacuum manifold for filtration microscopy 有权

    公开(公告)号：US12070725B2

    公开(公告)日：2024-08-27

    申请号：US18315911

    申请日：2023-05-11

    申请人： Optofluidics Inc.

    发明人： Thomas Castner ,  Robert Hart ,  Colby Ashcroft ,  Brian DiPaolo ,  Nathan Wall

    IPC分类号： B01D69/10 ,  G01N15/0227 ,  G01N15/14 ,  G06T7/00

    CPC分类号： B01D69/106 ,  G01N15/0227 ,  G01N15/14 ,  G06T7/001 ,  G01N2015/1493 ,  G06T2207/10056

    摘要： A vacuum manifold for filtration microscopy includes a manifold top having multiple openings, and a capture membrane positioned above and spaced apart from the manifold top, where the capture membrane is configured to deflect into contact with a surface of the manifold top when a negative pressure is applied to the multiple openings. A method for filtration microscopy includes the steps of providing a vacuum manifold including a manifold top having a plurality of openings, and a capture membrane positioned above and spaced apart from the manifold top; applying sample drops to sample spots on the membrane, the sample spots positioned above the plurality of openings; applying a negative pressure to the openings such that the capture membrane contacts a surface of the manifold top; and optically imaging particulates on the capture membrane.

    VACUUM MANIFOLD FOR FILTRATION MICROSCOPY
    10.
    发明申请
    VACUUM MANIFOLD FOR FILTRATION MICROSCOPY 有权

    公开(公告)号：US20210102881A1

    公开(公告)日：2021-04-08

    申请号：US17116203

    申请日：2020-12-09

    申请人： Optofluidics Inc.

    发明人： Thomas Castner ,  Robert Hart ,  Colby Ashcroft ,  Brian DiPaolo ,  Nathan Wall

    IPC分类号： G01N15/02 ,  G01N15/14 ,  G06T7/00

    摘要： A vacuum manifold for filtration microscopy includes a manifold top having multiple openings, and a capture membrane positioned above and spaced apart from the manifold top, where the capture membrane is configured to deflect into contact with a surface of the manifold top when a negative pressure is applied to the multiple openings. A method for filtration microscopy includes the steps of providing a vacuum manifold including a manifold top having a plurality of openings, and a capture membrane positioned above and spaced apart from the manifold top; applying sample drops to sample spots on the membrane, the sample spots positioned above the plurality of openings; applying a negative pressure to the openings such that the capture membrane contacts a surface of the manifold top; and optically imaging particulates on the capture membrane.