公开(公告)号：US20240127910A1

公开(公告)日：2024-04-18

申请号：US18204912

申请日：2023-06-01

申请人： The Regents of University of California

发明人： Colleen Clancy ,  Pei-Chi Yang ,  Kevin DeMarco ,  Igor V. Vorobyov

IPC分类号： G16C20/50 ,  G06N20/00 ,  G16C20/30 ,  G16C20/70 ,  G16H50/50

CPC分类号： G16C20/50 ,  G06N20/00 ,  G16C20/30 ,  G16C20/70 ,  G16H50/50

摘要： The disclosure presents a new computer based model framework to predict drug effects over multiple time and spatial scales from the drug chemistry to the cardiac rhythm. The disclosure presents a new computer based model framework to predict drug effects from the level of the receptor interaction to the cardiac rhythm.

公开(公告)号：US20190257808A1

公开(公告)日：2019-08-22

申请号：US16281036

申请日：2019-02-20

申请人： The Regents of the University of California

发明人： Chan Seung Park ,  Partho Roy

IPC分类号： G01N33/22 ,  F02D41/14 ,  G01N33/28

摘要： A new fuel quality sensor enables the use of RNG without removing CO2. Efficiency and the economy of the process improves significantly if CO2 separation cost can be avoided. Fuel quality information from the sensor makes it easy to adjust air/fuel ratio as well as efficient combustion inside a combustion engine or boiler.

公开(公告)号：US20060260620A1

公开(公告)日：2006-11-23

申请号：US11335392

申请日：2006-01-18

申请人： Homayoon Kazerooni ,  Nathan Harding ,  Russdon Angold

发明人： Homayoon Kazerooni ,  Nathan Harding ,  Russdon Angold

IPC分类号： A61G15/00

CPC分类号： A61B5/1038 ,  A61B5/4528 ,  A61B5/6829 ,  A61B2562/0219 ,  A61F5/0102 ,  B25J9/0006

摘要： A lower extremity exoskeleton, configurable to be coupled to a person, comprises two leg supports configurable to be coupled to the person's lower limbs and configured to rest on the ground during their stance phases. Each leg support comprises a thigh link, a shank link, and two knee joints. Each knee joint is configured to allow flexion and extension between the respective shank link and the respective thigh link. The lower extremity exoskeleton also comprises an exoskeleton trunk configurable to be coupled to the person's upper body. The exoskeleton trunk is rotatably connectable to the thigh links of the leg supports allowing for the flexion and extension between the leg supports and the exoskeleton trunk. In this exemplary embodiment, the energy required for flexion and extension movement between the shank link and the respective thigh link of a leg support over a cyclic knee motion is provided by the person.

摘要翻译： 可配置为耦合到人的下肢外骨骼包括可配置为联接到人的下肢并被配置为在其姿态阶段期间搁置在地面上的两个腿部支撑件。 每个腿部支撑件包括大腿连杆，小腿连杆和两个膝关节。 每个膝关节被构造成允许在相应的小腿连杆和相应的大腿连杆之间弯曲和伸展。 下肢外骨骼还包括可配置成与人的上身联接的外骨骼躯干。 外骨骼躯干可旋转地连接到腿部支撑件的大腿连接部，允许脚部支撑件和外骨骼躯干之间的弯曲和伸展。 在该示例性实施例中，由腿部支撑件和腿部支撑件的相应大腿连接件之间的弯曲和伸展运动所需的能量在循环膝盖运动上提供。

公开(公告)号：US20060078725A1

公开(公告)日：2006-04-13

申请号：US11143372

申请日：2005-06-01

申请人： Ronald Fearing ,  Kellar Autumn

发明人： Ronald Fearing ,  Kellar Autumn

IPC分类号： B32B5/16

CPC分类号： B81C1/00111 ,  B81C1/00206 ,  C09J7/00 ,  C09J2201/626 ,  G01N3/24 ,  G01N19/04 ,  G01N2203/0025 ,  G01N2203/0286 ,  G01N2203/0623 ,  Y10T428/24802 ,  Y10T428/25

摘要： A fabricated microstructure includes a base and one or more nano-structures disposed on one or more portions of the base to adhere to a contact surface. The one or more portions of the base with the one or more nano-structures are located on the base such that, when the one or more nano-structures adhere to the contact surface and an external force is applied to peel the base from the contact surface, the one or more nano-structures in the one or more portions of the base facilitate or resist peeling of the nano-structures from the contact surface.

摘要翻译： 制造的微结构包括基底和设置在基底的一个或多个部分上以粘附到接触表面的一个或多个纳米结构。 具有一个或多个纳米结构的基底的一个或多个部分位于基底上，使得当一个或多个纳米结构粘附到接触表面并且施加外力以从接触部剥离基部 基底的一个或多个部分中的一个或多个纳米结构促进或抵抗纳米结构从接触表面的剥离。

公开(公告)号：US20040091578A1

公开(公告)日：2004-05-13

申请号：US10698824

申请日：2003-10-30

申请人： The Regents of University of California

发明人： Bob B. Buchanan ,  Susumu Morigasaki ,  Gregorio del Val ,  Oscar L. Frick

IPC分类号： C12P021/06 ,  A23B007/10

CPC分类号： A21D2/26 ,  A21D2/264 ,  A21D2/265 ,  A21D2/266 ,  A21D2/36 ,  A21D8/04 ,  A21D13/064 ,  A23C9/1209 ,  A23C9/1213 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K20/147 ,  A23L5/27 ,  A23L7/109 ,  A23L11/34 ,  A23L13/48 ,  A23L33/17 ,  A61K38/00 ,  A61K39/35 ,  A61K2035/122 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/43504 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y108/01008 ,  C12Y108/01009

摘要： Disulfide proteins showed mitigated allergenicity and increased digestibility by pepsin following reduction by thioredoxin. The sulfhydryl groups newly formed on reduction by thioredoxin (at 4null C.) or dithiothreitol (DTT) (at 55null C.) were blocked with a physiological disulfide, such as cystamine or oxidized glutathione (GSSG) to obtain stable forms of the disarmed allergen. When derivatized with cystamine, BLG was separated from its oxidized and reduced forms on non-reducing SDS-PAGE and appeared to lack sulfhydryl groups. Although less effective GSSG, gave similar results. Allergenicity of the two derivatives was compared with that of the oxidized, reduced and reoxidized forms of BLG by skin testing dogs from a colony sensitized to cow's milk. Both the cystamine and GSSG derivatized BLG showed decreased allergenicity and increased sensitivity to pepsin as compared to controls. The reoxidized form resembled the derivatives in having lower allergenicity. The thioredoxin- and DTT-reduced forms showed hypoallergenic, hyperdigestible properties, most effectively when the reduced proteins were heated at 55null C. Whole milk subjected to these procedures showed results similar to those obtained with pure BLG. Other proteins are similarly stabilized. Stable forms of such disarmed, hypoallergenic and hyperdigestible disulfide protein allergens or just hypoallergenic or just hyperdigestible protein allergens are useful in foods as well as clinical preparations.

公开(公告)号：US5910403A

公开(公告)日：1999-06-08

申请号：US857007

申请日：1997-05-15

申请人： Marc K. Hellerstein

发明人： Marc K. Hellerstein

IPC分类号： G01N33/50 ,  A61K31/711 ,  A61K51/00 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/15 ,  G01N33/60 ,  C12Q1/00

CPC分类号： A61K31/665 ,  C12Q1/48 ,  C12Q1/68 ,  C12Q1/6809 ,  C12Q1/70 ,  G01N33/5005 ,  G01N33/5088 ,  G01N33/60

摘要： The present invention relates to methods for measuring the proliferation and destruction rates of cells by measuring deoxyribonucleic acid (DNA) synthesis. In particular, the methods utilize non-radioactive stable isotope labels to endogenously label DNA synthesized through the de novo nucleotide synthesis pathway in a cell. The amount of label incorporated in the DNA is measured as an indication of cellular proliferation. Such methods do not involve radioactivity or potentially toxic metabolites, and are suitable for use both in vitro and in vivo. Therefore, the invention is useful for measuring cellular proliferation in humans for the diagnosis of a variety of disease conditions in which cellular proliferation is involved.

摘要翻译： 本发明涉及通过测量脱氧核糖核酸（DNA）合成来测量细胞的增殖和破坏率的方法。 特别地，该方法利用非放射性稳定同位素标记来内生标记通过细胞中从头核苷酸合成途径合成的DNA。 测量结合在DNA中的标记量作为细胞增殖的指示。 这些方法不涉及放射性或潜在的毒性代谢物，并且适用于体外和体内。 因此，本发明可用于测量人类的细胞增殖以诊断涉及细胞增殖的各种疾病状况。

公开(公告)号：US5763162A

公开(公告)日：1998-06-09

申请号：US161231

申请日：1993-12-02

申请人： Alexander N. Glazer ,  Richard A. Mathies ,  Konan Peck

发明人： Alexander N. Glazer ,  Richard A. Mathies ,  Konan Peck

IPC分类号： C07D221/12 ,  C07D405/12 ,  C07D417/06 ,  C07D417/14 ,  C09B23/04 ,  C12Q1/68 ,  G01N27/447 ,  G01N27/26

CPC分类号： C07D417/14 ,  C07D221/12 ,  C07D405/12 ,  C07D417/06 ,  C09B23/04 ,  C12Q1/6816 ,  G01N27/44726 ,  G01N27/44747 ,  Y10T436/143333

摘要： Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.

摘要翻译： 提供新的荧光标记技术和荧光标记，采用高亲和力非共价结合和插入荧光染料和dsDNA。 染料可用于提供电泳凝胶中核酸的高灵敏度标记，以及预先制备的用于结合多种特异性结合对成员的标记。 DNA染料荧光剂复合物可用于诊断测定中的标记，特异性核酸序列的检测等。

公开(公告)号：US5552555A

公开(公告)日：1996-09-03

申请号：US475047

申请日：1995-06-06

申请人： Roger Y. Tsien ,  Stephen R. Adams

发明人： Roger Y. Tsien ,  Stephen R. Adams

IPC分类号： C07D277/28 ,  C07D413/04 ,  C07D413/14

CPC分类号： C07D413/04 ,  C07D413/14

摘要： The present invention relates to a group of organic chelators whose affinity for calcium ion in solution is decreased by electromagnetic radiation. Specifically, the chelators are related to fura-2 and utilize the addition of an azide group to the 3-position of the benzofuran ring of a fura-2 type structure. Photolysis of the azide group causes the calcium ion affinity to decrease 100 to 1000 fold. These chelators when incorporated into rat fibroblasts either by microinjection or by incubation as the membrane-permeable, enzymatically-labile esters and flash-photolyzed cause large increases in intracellular free calcium ion. These chelators are used to generate controlled fast elevation of intracellular free calcium ion concentration to mimic or modulate a number of important cellular responses, especially in nerve or muscle.

摘要翻译： 本发明涉及通过电磁辐射降低溶液中对钙离子的亲和力的一组有机螯合剂。 具体地说，螯合剂与fura-2相关，并且利用在fura-2型结构的苯并呋喃环的3-位上添加叠氮基。 叠氮化物基团的光解使钙离子亲和力降低100〜1000倍。 当这些螯合剂通过显微注射或通过温育作为膜可渗透的酶不稳定的酯并且闪光光解引入大鼠成纤维细胞时，引起细胞内游离钙离子的大量增加。 这些螯合剂用于产生受控的快速升高的细胞内游离钙离子浓度以模拟或调节许多重要的细胞反应，特别是在神经或肌肉中。

公开(公告)号：US4468742A

公开(公告)日：1984-08-28

申请号：US244761

申请日：1981-03-17

申请人： Donald J. Jenden ,  Robert W. Silverman ,  Frederick C. Lee

发明人： Donald J. Jenden ,  Robert W. Silverman ,  Frederick C. Lee

IPC分类号： G01N30/86 ,  G06F17/00 ,  G06F15/20

CPC分类号： G06K9/0053 ,  G01N30/8617 ,  G01N30/8634

摘要： Quantitative chromatography is widely used in many fields to measure the amount of a compound present in a mixture. A series of Gaussian-like peaks are generated as output, and the size is conventionally measured by peak area or peak height determinations. The microprocessor-based analysis system of the present invention is designed to "learn" the characteristics of a set of chromatographic peaks. Retention time and peak width parameters are used to detect and measure selected peaks by a convolution procedure based on a matched filter. The filter is applied to peaks in the neighborhood of selecting peak times which have a shape closely resembling that of a control sample. The system is built around a microprocessor with a wide dynamic range analog-to-digital conversion input stage. Operator interaction with the programs and the printing of output results is facilitated.

摘要翻译： 定量层析广泛用于许多领域以测量混合物中存在的化合物的量。 产生一系列高斯形峰作为输出，并且通常通过峰面积或峰高确定来测量尺寸。 本发明的基于微处理器的分析系统旨在“学习”一组色谱峰的特征。 保留时间和峰宽参数用于通过基于匹配滤波器的卷积过程来检测和测量所选择的峰。 滤波器被应用于选择峰值时间附近的峰值，其具有与对照样品的形状非常相似的形状。 该系统围绕具有宽动态范围模数转换输入级的微处理器构建。 操作员与程序的交互和输出结果的打印变得便利。

公开(公告)号：US20160290805A1

公开(公告)日：2016-10-06

申请号：US15038702

申请日：2014-12-02

申请人： THE REGENTS OF UNIVERSITY OF CALIFORNIA

发明人： Andrew T. Irish ,  Jason T. Isaacs ,  Francois Quitin ,  Joao P. Hespanha ,  Upamanyu Madhow

IPC分类号： G01C21/00 ,  G01S19/22

CPC分类号： G01C21/005 ,  G01S19/22

摘要： Various embodiments each include at least one of systems, methods, devices, and software for GNSS simultaneous localization and mapping (SLAM). The disclosed techniques demonstrate that simultaneous localization and mapping (SLAM) can be performed using only GNSS SNR and geo-location data, collectively termed GNSS data henceforth. A principled Bayesian approach for doing so is disclosed. A 3-D environment map is decomposed into a grid of binary-state cells (occupancy grid) and the receiver locations are approximated by sets of particles. Using a large number of sparsely sampled GNSS SNR measurements and receiver/satellite coordinates (all available from off-the-shelf GNSS receivers), likelihoods of blockage are associated with every receiver-to-satellite beam. Loopy Belief Propagation is used to estimate the probabilities of each cell being occupied or empty, along with the probability of the particles for each receiver location.

摘要翻译： 各种实施例各自包括用于GNSS同时定位和映射（SLAM）的系统，方法，设备和软件中的至少一个。 所公开的技术表明，可以仅使用GNSS SNR和地理位置数据（以下统称为GNSS数据）来执行同时定位和映射（SLAM）。 披露了一项原则性的贝叶斯方法。 3-D环境图被分解成二进制状态单元格（占用网格），并且接收器位置由一组粒子近似。 使用大量稀疏采样的GNSS SNR测量和接收机/卫星坐标（全部可从现成的GNSS接收机获得），阻塞的可能性与每个接收机到卫星的波束相关联。 Loopy信仰传播用于估计每个被占用或空的单元的概率，以及每个接收器位置的粒子的概率。