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1.发明授权Regulatory sequences of alcohol oxidase (MOX) and dihydroxyacetonesynthase (DAS) of Hansenula polymorpha 失效汉逊酵母（Hansenula polymorpha）的醇氧化酶（MOX）和二羟基丙酮合酶（DAS）的调控序列
公开(公告)号：US5240838A
公开(公告)日：1993-08-31
申请号：US587555
申请日：1990-09-24
申请人： Adrianus M. Ledeboer , Jan Maat , Cornelis T. Verrips , Christiaan Visser , Zbigniew A. Janowicz , Cornelis P. Hollenberg
发明人： Adrianus M. Ledeboer , Jan Maat , Cornelis T. Verrips , Christiaan Visser , Zbigniew A. Janowicz , Cornelis P. Hollenberg
IPC分类号： C07K14/60 , C11D3/386 , C12N9/02 , C12N9/04 , C12N9/06 , C12N9/10 , C12N15/68 , C12N15/81 , C12N15/90
CPC分类号： C12N9/0022 , C07K14/60 , C11D3/38654 , C12N15/68 , C12N15/81 , C12N15/815 , C12N15/90 , C12N9/0004 , C12N9/0006 , C12N9/1022 , A01K2217/05 , C07K2319/02 , C07K2319/75
摘要： The structural genes and their regulatory DNA sequences of an alcohol oxidase (MOX) and a dihydroxyacetone synthase (DHAS) of Hansenula polymorpha have been isolated and the nucleotide sequences determined. The invention relates to the use of the MOX gene, as well as the use of the regulatory DNA sequences of MOX and/or DAS in combination with the MOX gene, optionally after modification thereof, or other oxidase genes, or other genes, to produce engineered microorganisms, in particular yeasts. Said engineered microorganisms can produce oxidases or other enzymes in yields that allow industrial application on a large scale. Moreover, said engineered microorganisms can produce oxidases having improved properties with respect to their application in oxidation reactions and/or in bleaching and detergent products.
摘要翻译： 已经分离了多形汉逊酵母的醇氧化酶（MOX）和二羟基丙酮合成酶（DHAS）的结构基因及其调控DNA序列，确定了核苷酸序列。 本发明涉及MOX基因的用途，以及MOX和/或DAS的调节DNA序列与MOX基因组合使用，任选地在其修饰之后，或其它氧化酶基因或其他基因产生 工程微生物，特别是酵母菌。 所述工程微生物可以以允许大规模工业应用的产率产生氧化酶或其它酶。 此外，所述工程微生物可以产生具有相对于其在氧化反应和/或漂白和洗涤剂产品中的应用具有改进性能的氧化酶。
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2.发明授权Structural genes encoding the various allelic and maturation forms of preprothaumatin, recombinant cloning vehicles comprising said structural genes and expression thereof in transformed microbial host cells 失效编码前油麻黄素的各种等位基因和成熟形式的结构基因，包含所述结构基因的重组克隆载体及其在转化的微生物宿主细胞中的表达
公开(公告)号：US4771000A
公开(公告)日：1988-09-13
申请号：US732818
申请日：1985-05-10
IPC分类号： C12N15/09 , C07K14/43 , C12N1/20 , C12N1/21 , C12P19/34 , C12P21/00 , C12P21/02 , C12R1/19 , C07H21/04 , C12N15/00 , C12P21/04
CPC分类号： C07K14/43
摘要： The invention relates to structural genes comprising encoding non-processed and partly processed thaumatin, to the various allelic forms of said non-processed thaumatin and to recombinant DNA's and plasmids comprising said structural genes coding for the various allelic forms of preprothaumatin, and naturally and/or artifically modified preprothaumatin in various stages of its natural processing, and to the use of said recombinant plasmids to transform microorganisms, particularly bacteria in which said genes are expressed.
摘要翻译： 本发明涉及包含编码未处理和部分处理的奇异果甜蛋白的结构基因，与所述未加工的马齿苋的各种等位基因型以及包含编码前油麻黄素的各种等位基因形式的所述结构基因的重组DNA和质粒，天然和/ 或在其天然加工的各个阶段的人工修饰的前油麻黄素，以及使用所述重组质粒转化微生物，特别是表达所述基因的细菌。
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公开(公告)号：US5260212A
公开(公告)日：1993-11-09
申请号：US635965
申请日：1990-12-31
IPC分类号： A23L3/3571 , A61K38/00 , C07K14/195 , C07K14/41 , C12N1/19 , C12N1/21 , C12N15/00 , C12N15/09 , C12N15/31 , C12N15/70 , C12P21/02 , C12R1/01 , C12R1/19 , C12R1/865 , C12N15/63
CPC分类号： C12N15/70 , A23L3/3571 , C07K14/195 , A61K38/00
摘要： Isolation and identification of a gene encoding for a bacteriocin precursor in Pediococcus acidilactici, cloning of the gene in a vector plasmid and transformation to bacteria is described. The bacteriocin is particularly useful for inhibiting Listeria in food products.
摘要翻译： 描述了编码乳酸球菌的细菌素前体的基因的分离和鉴定，载体质粒中的基因的克隆和转化为细菌。 细菌素特别可用于抑制食品中的李斯特菌。
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4.发明授权Structural genes encoding the various allelic and maturation forms of preprothaumatin recombinant cloning vehicles, comprising said structural genes and expression thereof in transformed microbial host cells 失效编码前油麻黄素重组克隆载体的各种等位基因和成熟形式的结构基因，其包含所述结构基因和在转化的微生物宿主细胞中的表达
公开(公告)号：US4966842A
公开(公告)日：1990-10-30
申请号：US194923
申请日：1988-05-17
CPC分类号： C07K14/43
摘要： The invention relates to structural genes consisting of DNA sequences encoding non-processed and partly processed thaumatin, to the various allelic forms of said non-processed thaumatin and to recombinant DNA's and plasmids comprising said structural genes coding for the various allelic forms of preprothaumatin, and naturally and/or artificially modified preprothaumatin in various stages of its natural processing, and to the use of said recombinant plasmids to transform microorganisms, particularly bacteria in which said genes are expressed.
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公开(公告)号：US5175252A
公开(公告)日：1992-12-29
申请号：US841655
申请日：1992-02-25
IPC分类号： A23L3/3571 , A61K38/00 , C07K14/195 , C12N15/70
CPC分类号： A23L3/3571 , C07K14/195 , C12N15/70 , A61K38/00
摘要： Isolation and identification of a gene encoding for a bacteriocin precursor in Pediococcus acidilactici, cloning of the gene in a vector plasmid and transformation to bacteria is described. The bacteriocin is particularly useful for inhibiting Listeria in food products.
摘要翻译： 描述了编码乳酸球菌的细菌素前体的基因的分离和鉴定，载体质粒中的基因的克隆和转化为细菌。 细菌素特别可用于抑制食品中的李斯特菌。
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6.发明授权Salt-inducible promoter derivable from a lactic acid bacterium, and its use in a lactic acid bacterium for production of a desired protein 失效衍生自乳酸菌的盐诱导型促进剂，以及其在乳酸菌中用于产生所需蛋白质的用途
公开(公告)号：US6140078A
公开(公告)日：2000-10-31
申请号：US68195
申请日：1998-07-29
申请人： Jan W. Sanders , Jan Kok , Gerard Venema , Adrianus M Ledeboer
发明人： Jan W. Sanders , Jan Kok , Gerard Venema , Adrianus M Ledeboer
CPC分类号： C12N15/746 , C07K14/335
摘要： The invention provides a salt-inducible promoter present in SEQ ID NO: 10 and derivable from a lactic acid bacterium in isolation from the coding sequence normally controlled by said promoter in a wild-type lactic acid bacterium, with modifications and important parts thereof. Also provided are a recombinant vector and a transformed lactic acid bacterium comprising such promoter, and the production of a desired protein by such transformed bacterium, whereby the gene encoding said desired protein or a precursor thereof is expressed under control of such promoter. The desired protein can be secreted by the bacterium due to the presence of a signal sequence. The action of the salt-inducible promoter is enhanced at a pH of about 4-4.5 and/or by the presence of glutamic acid. Such process can be used in a fermentation process, in which the desired protein is a lytic protein causing lysis of the cells and release of the cell content. Or the desired protein can be an enzyme involved in flavour formation, e.g. in producing dressings, spreads, sausages and sour dough, or a protein functioning in a cheese production process, such as chymosin or a precursor thereof, or an enzyme involved in cheese flavour formation.
摘要翻译： PCT No.PCT / EP97 / 04755 Sec。 371日期1998年7月29日第 102（e）日期1998年7月29日PCT 1997年8月20日PCT PCT。 公开号WO98 / 10080 PCT 日期1998年3月12日本发明提供了存在于SEQ ID NO：10中的衍生自乳酸菌的盐诱导型启动子，其与野生型乳酸菌通常由所述启动子控制的编码序列分离， 其重要部分。 还提供了包含这种启动子的重组载体和转化的乳酸菌，并且通过这种转化细菌产生所需的蛋白质，由此编码所述所需蛋白质或其前体的基因在这种启动子的控制下表达。 由于信号序列的存在，所需的蛋白质可被细菌分泌。 在约4-4.5的pH和/或谷氨酸的存在下，盐诱导型启动子的作用增强。 这种方法可用于发酵过程中，其中所需蛋白质是引起细胞裂解和释放细胞内容物的裂解蛋白。 或者所需的蛋白质可以是参与风味形成的酶，例如 在制作敷料，酱油，香肠和酸面团中，或在酪乳生产过程中起作用的蛋白质，例如凝乳酶或其前体，或涉及奶酪香味形成的酶。
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7.发明授权Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes 失效使用多形汉逊酵母MOX和DAS基因的启动子表达和产生多肽
公开(公告)号：US5741672A
公开(公告)日：1998-04-21
申请号：US473295
申请日：1995-06-07
申请人： Adrianus M. Ledeboer , Jan Maat , Cornelis T. Verrips , Christiaan Visser , Zbigniew A. Janowicz , Cornelis P. Hollenberg
发明人： Adrianus M. Ledeboer , Jan Maat , Cornelis T. Verrips , Christiaan Visser , Zbigniew A. Janowicz , Cornelis P. Hollenberg
CPC分类号： C12N9/0006 , C11D3/38654 , C12N9/1022 , A01K2217/05
摘要： The structural genes and their regulatory DNA sequences of an alcohol oxidase (MOX) and a dihydroxyacetone synthase (DHAS) of Hansenula polymorpha have been isolated and the nucleotide sequences determined. The invention relates to the use of the MOX gene, as well as the use of the regulatory DNA sequences of MOX and/or DAS in combination with the MOX gene, optionally after modification thereof, or other oxidase genes, or other genes, to produce engineered microorganisms, 0in particular yeasts. Said engineered microorganisms can produce oxidases or other enzymes in yields that allow industrial application on a large scale. Moreover, said engineered microorganisms can produce oxidases having improved properties with respect to their application in oxidation reactions and/or in bleaching and detergent products.
摘要翻译： 已经分离了多形汉逊酵母的醇氧化酶（MOX）和二羟基丙酮合成酶（DHAS）的结构基因及其调控DNA序列，确定了核苷酸序列。 本发明涉及MOX基因的用途，以及MOX和/或DAS的调节DNA序列与MOX基因组合使用，任选地在其修饰之后，或其它氧化酶基因或其他基因产生 工程微生物，在特定酵母中。 所述工程微生物可以以允许大规模工业应用的产率产生氧化酶或其它酶。 此外，所述工程微生物可以产生具有相对于其在氧化反应和/或漂白和洗涤剂产品中的应用具有改进性能的氧化酶。
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8.发明授权DNA sequences encoding the various allelic forms of mature thaumatin, and cloning vehicles, etc. 失效编码成熟的马蹄拟糖的各种等位基因形式的DNA序列和克隆载体等
公开(公告)号：US4891316A
公开(公告)日：1990-01-02
申请号：US742139
申请日：1985-06-07
IPC分类号： C12N15/09 , C07H21/04 , C07K14/43 , C12N1/20 , C12N1/21 , C12N15/31 , C12N15/66 , C12N15/70 , C12P19/34 , C12P21/00 , C12P21/02 , C12R1/19
摘要： The invention relates to DNA sequences encoding the various allelic forms of mature thaumatin, and cloning vehicles comprising said DNA sequences and their use in transforming microorganisms.
摘要翻译： 本发明涉及编码成熟的马蹄拟糖的各种等位基因形式的DNA序列，以及包含所述DNA序列的克隆载体及其在转化微生物中的用途。
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