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    STERILITY INDICATING BIOLOGICAL COMPOSITIONS, ARTICLES AND METHODS
    1.
    发明申请
    STERILITY INDICATING BIOLOGICAL COMPOSITIONS, ARTICLES AND METHODS 审中-公开
    表明生物组成的无效性，文章和方法

    公开(公告)号：US20110195442A1

    公开(公告)日：2011-08-11

    申请号：US13123824

    申请日：2009-10-15

    申请人： Sailaja Chandrapati ,  Heather M. Webb ,  Ai-Ping Wei

    发明人： Sailaja Chandrapati ,  Heather M. Webb ,  Ai-Ping Wei

    IPC分类号： C12Q1/37

    CPC分类号： C12Q1/37 ,  A61L2/28 ,  C12Q1/22

    摘要： A sterility indicating composition comprising a plurality of sterilization process resistant spores which contain an active protease during germination and initial outgrowth of the spores; and a germination medium comprising at least one labeled protease substrate and at least one nutrient for germination of the spores; wherein the medium is essentially free of a) any active protease other than the active protease contained by the plurality of spores and b) any protease substrate other than the at least one labeled protease substrate, other than any protease substrate originating from the plurality of spores, and other than any protease substrate which does not compete with the labeled protease substrate for the active protease; and wherein the at least one labeled protease substrate comprises a peptide which can be cleaved by the active protease and which is labeled with one or more dye groups, at least one of which undergoes a detectable change when the peptide is cleaved by the active protease, and wherein the labeled protease substrate is stable at least at a temperature for incubating the spores, a sterilization process indicator comprising the composition, and a method of determining the effectiveness of a sterilization process using the composition and indicator are disclosed.

    摘要翻译： 一种不育指示组合物，其包含多个灭菌过程抗性孢子，其在萌发期间含有活性蛋白酶和孢子的初始产生; 以及包含至少一种标记的蛋白酶底物和至少一种用于孢子发芽的营养物的发芽培养基; 其中所述培养基基本上不含a）除所述多个孢子所含的活性蛋白酶以外的任何活性蛋白酶，和b）除所述至少一个标记的蛋白酶底物之外的任何蛋白酶底物，而不是源于所述多个孢子的任何蛋白酶底物 ，而不是与活性蛋白酶的标记的蛋白酶底物竞争的任何蛋白酶底物以外的其它蛋白酶底物; 并且其中所述至少一个标记的蛋白酶底物包含可被活性蛋白酶切割并且被一个或多个染料基团标记的肽，当肽被活性蛋白酶切割时，其至少一种经历可检测的变化， 并且其中标记的蛋白酶底物至少在用于培养孢子的温度下是稳定的，包括该组合物的灭菌过程指示剂以及使用该组合物和指示剂确定灭菌过程的有效性的方法。

    Fluorogenic protease substrates based on dye-dimerization
    3.
    发明授权
    Fluorogenic protease substrates based on dye-dimerization 失效
    基于染料二聚化的荧光蛋白酶底物

    公开(公告)号：US07256012B2

    公开(公告)日：2007-08-14

    申请号：US10930522

    申请日：2004-08-31

    申请人： Ai-Ping Wei ,  Michael George Williams

    发明人： Ai-Ping Wei ,  Michael George Williams

    IPC分类号： C12Q1/37 ,  C12Q1/04 ,  C07K7/06 ,  G01N2/64

    CPC分类号： C07K7/06 ,  C12Q1/37 ,  C12Q2337/40 ,  Y02A50/451

    摘要： A method of biological assay comprises the steps of providing an enzyme substrate comprising two fluorescence dye groups bound to a flexible peptide, the dye groups being of proximity sufficiently close so as to allow free energy attractions to draw the dyes together to essentially self-quench fluorescence of the dye groups, wherein self quenching of fluorescence of the dye groups is effected by dye dimerization or stacking, and enzymatically cleaving the peptide to release the fluorescence dye groups from dye dimerization or stacking, thereby producing an increase in fluorescence intensity. A protease substrate for use in the method of the invention is also disclosed. This invention finds use in detection and identification of microorganisms, sterilization assurance, pharmaceutical discovery, enzyme assays, immunoassays, and other biological assays.

    摘要翻译： 一种生物测定方法包括以下步骤：提供包含与柔性肽结合的两个荧光染料基团的酶底物，所述染料组的接近度足够接近，以便使自由能吸引力将染料一起吸收到基本上自熄灭荧光 的染料基团，其中染料基团的荧光自发淬灭通过染料二聚化或堆叠进行，并且酶切割肽以使荧光染料基团从染料二聚化或堆叠释放，从而产生荧光强度的增加。 还公开了用于本发明方法的蛋白酶底物。 本发明用于微生物的检测和鉴定，灭菌保证，药物发现，酶测定，免疫测定和其他生物测定。

    Fluorogenic protease substrates based on dye-dimerization
    6.
    发明授权
    Fluorogenic protease substrates based on dye-dimerization 失效
    基于染料二聚化的荧光蛋白酶底物

    公开(公告)号：US06787329B1

    公开(公告)日：2004-09-07

    申请号：US09448633

    申请日：1999-11-24

    申请人： Ai-Ping Wei ,  Michael George Williams

    发明人： Ai-Ping Wei ,  Michael George Williams

    IPC分类号： C12Q137

    CPC分类号： C07K7/06 ,  C12Q1/37 ,  C12Q2337/40 ,  Y02A50/451

    摘要： A method of biological assay comprises the steps of providing an enzyme substrate comprising two fluorescence dye groups bound to a flexible peptide, the dye groups being of proximity sufficiently close so as to allow free energy attractions to draw the dyes together to essentially self-quench fluorescence of the dye groups, wherein self quenching of fluorescence of the dye groups is effected by dye dimerization or stacking, and enzymatically cleaving the peptide to release the fluorescence dye groups from dye dimerization or stacking, thereby producing an increase in fluorescence intensity. A protease substrate for use in the method of the invention is also disclosed. This invention finds use in detection and identification of microorganisms, sterilization assurance, pharmaceutical discovery, enzyme assays, immunoassays, and other biological assays.

    摘要翻译： 一种生物测定方法包括以下步骤：提供包含与柔性肽结合的两个荧光染料基团的酶底物，所述染料组的接近度足够接近，以便使自由能吸引力将染料一起吸收到基本上自熄灭荧光 的染料基团，其中染料基团的荧光自发淬灭通过染料二聚化或堆叠进行，并且酶切割肽以使荧光染料基团从染料二聚化或堆叠释放，从而产生荧光强度的增加。 还公开了用于本发明方法的蛋白酶底物。 本发明用于微生物的检测和鉴定，灭菌保证，药物发现，酶测定，免疫测定和其他生物测定。

    Immunoassay procedure utilizing fluorogenic tracer antigens
    8.
    发明授权
    Immunoassay procedure utilizing fluorogenic tracer antigens 失效
    使用荧光示踪剂抗原的免疫测定方法

    公开(公告)号：US06482655B1

    公开(公告)日：2002-11-19

    申请号：US08096338

    申请日：1993-07-23

    申请人： Ai-Ping Wei ,  James N. Herron

    发明人： Ai-Ping Wei ,  James N. Herron

    IPC分类号： G01N3353

    CPC分类号： G01N33/582 ,  C12Q1/6818 ,  Y10S436/80 ,  Y10S436/805

    摘要： Disclosed are fluorescent energy transfer dyes which are capable of moving between a more stacked configuration to exhibit fluorescent quenching and a more spaced configuration to exhibit fluorescence can be conjugated to a peptide epitope for use in the detection of an unknown antibody in bulk solution. The resulting labeled peptide reagent can be used in an immunoassay procedure by placing it in bulk solution along with the unknown antibody to be detected. When the antibody binds to the peptide epitope, the pair of dyes carried by the peptide epitope will have their configuration altered from a stacked to an unstacked configuration and will exhibit a fluorescent increase in response thereto.

    摘要翻译： 公开了能够在更叠层的配置之间移动以展示荧光猝灭和更间隔的构型以显示荧光的荧光能量转移染料可以缀合到肽表位，以用于检测本体溶液中的未知抗体。 所得标记的肽试剂可以在免疫测定程序中使用，将其与待检测的未知抗体一起放入本体溶液中。 当抗体与肽表位结合时，由肽表位携带的一对染料将具有从堆叠到未堆叠构型的改变，并且对其反应将呈现荧光增加。

    Method of determining energy transfer efficiency of a donor-acceptor pair of fluorescent dyes
    9.
    发明授权
    Method of determining energy transfer efficiency of a donor-acceptor pair of fluorescent dyes 失效
    确定荧光染料供体 - 受体对能量转移效率的方法

    公开(公告)号：US5237515A

    公开(公告)日：1993-08-17

    申请号：US683146

    申请日：1991-04-10

    申请人： James N. Herron ,  Ai-Ping Wei

    发明人： James N. Herron ,  Ai-Ping Wei

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6428 ,  G01N2021/6421 ,  G01N2201/129

    摘要： The energy transfer efficiency of a donor-acceptor pair of fluorescent dyes can be determined by first measuring the fluorescence and absorption spectra of donor-protein, acceptor-protein, mixture of donor-protein and acceptor-protein, and donor-acceptor-protein conjugates, then separating the respective spectra into their respective donor-protein complex and acceptor-protein complex components using multiple linear regression, and then determining the transfer efficiency on the basis of the quenching of the donor fluorescence based on the spectral data thus obtained.

    摘要翻译： 供体 - 受体对荧光染料的能量转移效率可以通过首先测量供体 - 蛋白质，受体 - 蛋白质，供体 - 蛋白质和受体 - 蛋白质的混合物和供体 - 受体 - 蛋白质缀合物的荧光和吸收光谱来确定 ，然后使用多元线性回归将相应的光谱分离成它们各自的供体 - 蛋白质复合物和受体 - 蛋白质复合物组分，然后基于由此获得的光谱数据基于供体荧光的猝灭确定转移效率。