摘要:
The present invention provides antibodies that bind to the endospore of the bacterium Clostridium difficile, methods of making such antibodies, and methods of using such antibodies, including methods of detecting C. difficile endospores.
摘要:
A method of determining the quality of oil by irradiating the oil at a first wavelength, e.g., of about 470 nm, measuring a level of fluorescence of the oil at a second wavelength, e.g., of about 520 nm, comparing the measured fluorescence level to a predetermined threshold level to determine whether or not the oil quality is acceptable. The oil is preferably discarded if the measured fluorescence level exceeds the predetermined threshold level, which can be generally dependent on the oil composition. The fluorescence is correlated to the level of polar components in the oil. Also described are devices for determining the quality of oil via fluorescence.
摘要:
The present invention provides antibodies that bind to the endospore of the bacterium Clostridium difficile, methods of making such antibodies, and methods of using such antibodies, including methods of detecting C. difficile endospores.
摘要:
Systems and methods for detecting an analyte of interest in a sample. The system can include a first container comprising a filter portion. The filter portion can include a filter comprising a filtrand of the sample on a first side of the filter. The system can further include a second container comprising the filter portion coupled to a detection portion comprising a microstructured surface. The method can include providing the first container, coupling the filter portion to the detection portion to form the second container where the first side of the filter faces the microstructured surface, and centrifuging the second container toward the microstructured surface. The method can further include inverting the second container after centrifuging to decant the supernatant from the detection portion, such that a concentrate comprising a sediment of the sample is retained in the microstructured surface, which can be interrogated for an analyte of interest.
摘要:
A system and method for preparing and collecting samples for analyte testing. The system can include a sample preparation system and a sample collection system coupled to the sample preparation system. The sample preparation system can include at least one of a deformable self-supporting receptacle comprising a reservoir and a freestanding receptacle comprising a reservoir. The reservoir can be adapted to contain a liquid composition. The sample collection system can be positioned in fluid communication with a reservoir of the sample preparation system, and can be adapted to capture an analyte of interest. The method can include providing a fluid path defined at least partially by the sample preparation system and the sample collection system, positioning the liquid composition in a reservoir of the sample preparation system, and moving at least a portion of the liquid composition in the fluid path to the sample collection system.
摘要:
Articles are provided for the detection of cells in a sample. The articles include a release element comprising a cell extractant. The release element includes a shell structure that controls the release of the cell extractant into a liquid mixture containing the sample. Methods of use are also disclosed.
摘要:
A method for isolating microorganisms from a sample, the sample including sample matrix and microorganisms, the method including the steps of providing a receptacle, the receptacle configured to allow filtering of the sample and to reversibly contain the sample and a concentration agent; adding the sample to the receptacle, wherein a microorganism-bound composition will be formed in the receptacle, the microorganism-bound composition including concentration agent-bound microorganisms and sample matrix; and filtering the microorganism-bound composition through a filter to collect the concentration agent-bound microorganisms on the filter, wherein the filter has an average pore size that is greater than the average size of the microorganisms. Kits and systems are also disclosed herein.
摘要:
The application discloses embodiments of detection devices including a sensor component in a flow path between a first flow path portion and a second flow path portion. In embodiments described, the sensor component includes a receptor in a polymerized composition. The receptor is configured to bind with an analyte in a test sample. Upon binding the sensor component undergoes a detectable change in response to interaction of the analyte with the receptor.
摘要:
Sample processing devices with electrophoresis channels and methods of loading the electrophoresis channels with electrophoresis sieving polymer while rotating the sample processing device are disclosed. In some instances, the electrophoresis channels may be arranged radially relative to the axis of rotation of the sample processing device. In other sample processing devices, the electrophoresis channels may be arranged in curved arcs that are concentric about the center of the sample processing device (which preferably corresponds to the axis of rotation).
摘要:
Method for detecting an analyte of interest in a sample. The method can include providing a container comprising a microstructured surface, and centrifuging the container toward the microstructured surface to form a sediment and a supernatant of the sample. Following centrifugation, the container can be inverted to decant at least a portion of the supernatant of the sample from the second portion, such that a concentrate (e.g., comprising the sediment) of the sample is retained in the microstructured surface. The concentrate can then be interrogated in the microstructured surface for the analyte of interest. In some embodiments, at least a portion of the second portion can be substantially transparent, such that the concentrate can be interrogated from the outside of the container, without requiring that the container be opened prior to interrogation.