公开(公告)号：US09493523B2

公开(公告)日：2016-11-15

申请号：US13810025

申请日：2011-05-06

申请人： Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi ,  Mikiko Nakamura

发明人： Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi ,  Mikiko Nakamura

IPC分类号： C07K14/47 ,  C12N9/00

CPC分类号： C07K14/47 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2318/20 ,  C12N9/93 ,  C12Y603/02019

摘要： Provided is an antigen-binding protein prepared merely by a method of in vitro selection using the RNF8-FHA domain, which has no intramolecular disulfide bond and functions in cells as it is. One to four loops extending from the FHA domain are randomized, and a recognition site for a target molecule is artificially created on the FHA domain surface to construct an RNF8-FHA domain library. Using the library, an antigen-binding protein is efficiently selected in vitro.

摘要翻译： 本发明提供了仅通过使用RNF8-FHA结构域进行体外选择的方法制备的抗原结合蛋白，其不具有分子内二硫键并且在细胞中起作用。 从FHA结构域延伸一至四个环被随机化，并在FHA结构域表面人工产生靶分子的识别位点以构建RNF8-FHA结构域文库。 使用文库，体外有效选择抗原结合蛋白。

公开(公告)号：US20130143299A1

公开(公告)日：2013-06-06

申请号：US13810025

申请日：2011-05-06

申请人： Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi ,  Mikiko Nakamura

发明人： Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi ,  Mikiko Nakamura

IPC分类号： C07K14/47

CPC分类号： C07K14/47 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2318/20 ,  C12N9/93 ,  C12Y603/02019

摘要： Provided is an antigen-binding protein prepared merely by a method of in vitro selection using the RNF8-FHA domain, which has no intramolecular disulfide bond and functions in cells as it is. One to four loops extending from the FHA domain are randomized, and a recognition site for a target molecule is artificially created on the FHA domain surface to construct an RNF8-FHA domain library. Using the library, an antigen-binding protein is efficiently selected in vitro.

摘要翻译： 本发明提供了仅通过使用RNF8-FHA结构域进行体外选择的方法制备的抗原结合蛋白，其不具有分子内二硫键并且在细胞中起作用。 从FHA结构域延伸一至四个环被随机化，并在FHA结构域表面人工产生靶分子的识别位点以构建RNF8-FHA结构域文库。 使用文库，体外有效选择抗原结合蛋白。

公开(公告)号：US06384532B1

公开(公告)日：2002-05-07

申请号：US09415106

申请日：1999-10-08

申请人： Toshihiro Yoshioka ,  Akira Miyakoshi

发明人： Toshihiro Yoshioka ,  Akira Miyakoshi

IPC分类号： H01J1749

CPC分类号： H01J11/12 ,  H01J9/02 ,  H01J11/38 ,  H01J2211/245

摘要： A plasma display panel having an improved display quality with low power consumption is provided. Pairs of first and second sustain electrodes are formed on the inner surface of a first substrate to extend in a first direction. A first dielectric layer is formed on the inner surface of the first substrate to cover the pairs of first and second sustain electrodes. Selection electrodes are formed on the inner surface of a second substrate to extend in a second direction perpendicular to the first direction. A second dielectric layer is formed on the inner surface of the second substrate to cover the selection electrodes. Partition walls are formed in the gap between the first and second substrates to extend in the second direction, thereby forming discharge spaces in the gap. Fluorescent layers are formed in the respective discharge spaces. A discharge gas is introduced in the discharge spaces. An overlapping part of the first dielectric layers with the first sustain electrodes has a non-uniform thickness in the widthwise direction of the first sustain electrode. An overlapping part of the first dielectric layers with the second sustain electrodes has a non-uniform thickness in the widthwise direction of the second sustain electrode. The non-uniform thickness is realized by protrusions or depressions for the first dielectric layer.

摘要翻译： 提供了一种具有改善的具有低功耗的显示质量的等离子体显示面板。 一对第一和第二维持电极形成在第一基板的内表面上以沿第一方向延伸。 在第一基板的内表面上形成第一介电层，以覆盖成对的第一和第二维持电极。 选择电极形成在第二基板的内表面上，以在与第一方向垂直的第二方向上延伸。 在第二基板的内表面上形成第二电介质层以覆盖选择电极。 分隔壁形成在第一和第二基板之间的间隙中，以在第二方向上延伸，从而在间隙中形成放电空间。 荧光层形成在各个放电空间中。 在放电空间中引入放电气体。 第一电介质层与第一维持电极的重叠部分在第一维持电极的宽度方向上具有不均匀的厚度。 第一电介质层与第二维持电极的重叠部分在第二维持电极的宽度方向上具有不均匀的厚度。 通过用于第一介电层的突起或凹陷来实现不均匀的厚度。

公开(公告)号：US20130143773A1

公开(公告)日：2013-06-06

申请号：US13806470

申请日：2011-06-23

申请人： Takuya Ueda ,  Takashi Kanamori ,  Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi

发明人： Takuya Ueda ,  Takashi Kanamori ,  Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi

IPC分类号： C12N15/10

CPC分类号： C12N15/1065 ,  C12N15/1041 ,  C12N15/1093 ,  C12N15/67

摘要： The present invention provides a method of producing a maturated oligonucleotide library, including a step of obtaining a terminal-modified product of a maturation target oligonucleotide library, including adding a tag sequence to the 5′ terminus of the maturation target oligonucleotide library and an arrest sequence, which stalls translation elongation on a ribosome, to the 3′ terminus of the maturation target oligonucleotide library, a step of transcribing the terminal-modified sequence product to give a transcript, and a step of in vitro translation for translating the transcript in vitro, wherein the maturation target oligonucleotide library is a random oligonucleotide library.

摘要翻译： 本发明提供一种生产成熟寡核苷酸文库的方法，包括获得成熟靶寡核苷酸文库的末端修饰产物的步骤，包括向成熟靶寡核苷酸文库的5'末端加入标签序列， ，其将核糖体上的翻译延伸失活到成熟靶寡核苷酸文库的3'末端，转录末端修饰的序列产物以产生转录物的步骤，以及用于体外翻译转录物的体外翻译步骤， 其中成熟靶寡核苷酸文库是随机寡核苷酸文库。

公开(公告)号：US06734626B2

公开(公告)日：2004-05-11

申请号：US09910792

申请日：2001-07-24

申请人： Toshihiro Yoshioka ,  Akira Miyakoshi

发明人： Toshihiro Yoshioka ,  Akira Miyakoshi

IPC分类号： H01J1749

CPC分类号： H01J11/12 ,  H01J11/24 ,  H01J11/38 ,  H01J2211/245

摘要： In order to improve the light emitting efficiency of a surface-discharge type color PDP, a sustaining electrode is divided into a plurality of electrodes formed in respective different layers. Lower electrodes 121 and upper electrodes 122 in different layers are formed as electrode pairs, an upper dielectric layer 14 is formed on the upper electrodes 122 in an upper layer to make a dielectric layer on the surface-discharge electrode pair thin to thereby maintain the discharge sustaining voltage low and obtain high light emitting efficiency.

摘要翻译： 为了提高表面放电型彩色PDP的发光效率，维持电极被分成形成在各个不同层中的多个电极。 不同层中的下电极121和上电极122形成为电极对，在上电极122上形成上电介质层14，以使表面放电电极对上的电介质层变薄，从而保持放电 维持电压低，发光效率高。

公开(公告)号：US09133452B2

公开(公告)日：2015-09-15

申请号：US13806470

申请日：2011-06-23

申请人： Takuya Ueda ,  Takashi Kanamori ,  Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi

发明人： Takuya Ueda ,  Takashi Kanamori ,  Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi

IPC分类号： C12N15/10 ,  C12N15/67

CPC分类号： C12N15/1065 ,  C12N15/1041 ,  C12N15/1093 ,  C12N15/67

摘要： The present invention provides a method of producing a maturated oligonucleotide library, including a step of obtaining a terminal-modified product of a maturation target oligonucleotide library, including adding a tag sequence to the 5′ terminus of the maturation target oligonucleotide library and an arrest sequence, which stalls translation elongation on a ribosome, to the 3′ terminus of the maturation target oligonucleotide library, a step of transcribing the terminal-modified sequence product to give a transcript, and a step of in vitro translation for translating the transcript in vitro, wherein the maturation target oligonucleotide library is a random oligonucleotide library.

摘要翻译： 本发明提供一种生产成熟寡核苷酸文库的方法，包括获得成熟靶寡核苷酸文库的末端修饰产物的步骤，包括向成熟靶寡核苷酸文库的5'末端加入标签序列， ，其将核糖体上的翻译延伸失活到成熟靶寡核苷酸文库的3'末端，转录末端修饰的序列产物以产生转录物的步骤，以及用于体外翻译转录物的体外翻译步骤， 其中成熟靶寡核苷酸文库是随机寡核苷酸文库。

公开(公告)号：US06852646B2

公开(公告)日：2005-02-08

申请号：US10190510

申请日：2002-07-09

申请人： Toshihiro Yoshioka ,  Akira Miyakoshi

发明人： Toshihiro Yoshioka ,  Akira Miyakoshi

IPC分类号： C03C17/00 ,  C23C10/28 ,  C23C24/10 ,  C23C26/02 ,  H01J9/02 ,  H01J11/22 ,  H01J11/34 ,  H01J11/38 ,  H01L21/8238

CPC分类号： C23C26/02 ,  C03C17/001 ,  C23C10/28 ,  C23C24/10 ,  H01J9/02 ,  H01J2211/38

摘要： A method for forming a dielectric film in a PDP includes the steps of: reducing the ambient pressure of an insulating film including a dielectric material before the ambient temperature reaches the reaction temperature of the dielectric material; introducing heated gas to increase the ambient pressure up to the atmospheric pressure while maintaining the ambient temperature at the reaction temperature; and lowering the ambient temperature down to the solidifying temperature of the insulating film while maintaining the atmospheric ambient pressure.

摘要翻译： 在PDP中形成电介质膜的方法包括以下步骤：在环境温度达到介电材料的反应温度之前降低包括介电材料的绝缘膜的环境压力; 引入加热气体以将环境压力升高至大气压力，同时将环境温度保持在反应温度; 并且在保持大气环境压力的同时将环境温度降低到绝缘膜的凝固温度。