公开(公告)号：US07425700B2

公开(公告)日：2008-09-16

申请号：US11178262

申请日：2005-07-08

Applicant: John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

Inventor： John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

IPC: H01J49/04 ,  H01J49/10 ,  B01D59/44

CPC classification number: G01N33/50 ,  A61B5/150755 ,  G01N27/26 ,  G06F19/18 ,  G06F19/24 ,  H01J49/00 ,  H01J49/0027 ,  H01J49/165 ,  Y02A90/22 ,  Y02A90/26 ,  Y10T436/117497

Abstract: The present invention relates to systems and methods for identifying new markers, diagnosing patients with a biological state of interest, and marketing/commercializing such diagnostics. The present invention relates to systems and methods of greater sensitivity, specificity, and/or cost effectiveness.

Abstract translation: 本发明涉及用于鉴定新标记，诊断具有生物学感兴趣的患者的系统和方法，以及销售/商业化这种诊断。 本发明涉及具有更大灵敏度，特异性和/或成本效益的系统和方法。

公开(公告)号：US20140374584A1

公开(公告)日：2014-12-25

申请号：US14166626

申请日：2014-01-28

Applicant: John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

Inventor： John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

IPC: G01N33/50 ,  G01N27/26 ,  A61B5/15 ,  H01J49/00

CPC classification number: G01N33/50 ,  A61B5/150755 ,  G01N27/26 ,  G16B20/00 ,  G16B40/00 ,  H01J49/00 ,  H01J49/0027 ,  H01J49/165 ,  Y02A90/22 ,  Y02A90/26 ,  Y10T436/117497

Abstract: A business method for use in classifying patient samples. The method includes steps of collecting case samples representing a clinical phenotypic state and control samples representing patients without said clinical phenotypic state. Preferably the system uses a mass spectrometry platform system to identify patterns of polypeptides in said case samples and in the control samples without regard to the specific identity of at least some of said polypeptides. Based on identified representative patterns of the state, the business method provides for the marketing of diagnostic products using representative patterns. The present invention relates to systems and methods for identifying new markers, diagnosing patients with a biological state of interest, and marketing/commercializing such diagnostics. The present invention relates to systems and methods of greater sensitivity, specificity, and/or cost effectiveness.

Abstract translation: 用于分类患者样本的商业方法。 该方法包括收集代表临床表型状态的病例样本和对照样品的步骤，所述样品代表没有所述临床表型状态的患者。 优选地，系统使用质谱平台系统来鉴定所述病例样品和对照样品中多肽的模式，而不考虑至少一些所述多肽的特异性同一性。 基于确定的状态的代表性模式，业务方法使用代表性模式提供诊断产品的营销。 本发明涉及用于鉴定新标记，诊断具有生物学感兴趣的患者的系统和方法，以及销售/商业化这种诊断。 本发明涉及具有更大灵敏度，特异性和/或成本效益的系统和方法。

公开(公告)号：US07906758B2

公开(公告)日：2011-03-15

申请号：US12172988

申请日：2008-07-14

Applicant: John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

Inventor： John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

IPC: B01D59/44

CPC classification number: G01N33/50 ,  A61B5/150755 ,  G01N27/26 ,  G06F19/18 ,  G06F19/24 ,  H01J49/00 ,  H01J49/0027 ,  H01J49/165 ,  Y02A90/22 ,  Y02A90/26 ,  Y10T436/117497

Abstract: The present invention relates to a charged particle beam apparatus which employs a scanning electron microscope for sample inspection and defect review. The present invent provides solution of improving imaging resolution by utilizing a field emission cathode tip with a large tip radius, applying a large accelerating voltage across ground potential between the cathode and anode, positioning the beam limit aperture before condenser lens, utilizing condenser lens excitation current to optimize image resolution, applying a high tube bias to shorten electron travel time, adopting and modifying SORIL objective lens to ameliorate aberration at large field of view and under electric drifting and reduce the urgency of water cooling objective lens while operating material analysis. The present invent provides solution of improving throughput by utilizing fast scanning ability of SORIL and providing a large voltage difference between sample and detectors.

Abstract translation: 本发明涉及采用扫描电子显微镜进行样品检查和缺陷检查的带电粒子束装置。 本发明提供了通过利用具有大的尖端半径的场致发射阴极尖端来提高成像分辨率的解决方案，在阴极和阳极之间的地电位上施加大的加速电压，将光束极限孔定位在聚光透镜之前，利用聚光透镜激发电流 优化图像分辨率，应用高管偏压缩短电子行进时间，采用和修正SORIL物镜，在大视场和电漂移下改善像差，降低水冷物镜在操作材料分析时的紧迫性。 本发明提供了通过利用SORIL的快速扫描能力并在样品和检测器之间提供大的电压差来提高产量的解决方案。

公开(公告)号：US20060027744A1

公开(公告)日：2006-02-09

申请号：US11178262

申请日：2005-07-08

Applicant: John Stults ,  Alfnd Greenquist ,  Alexander Sassi

Inventor： John Stults ,  Alfnd Greenquist ,  Alexander Sassi

IPC: B01D59/44 ,  H01J49/00

CPC classification number: G01N33/50 ,  A61B5/150755 ,  G01N27/26 ,  G06F19/18 ,  G06F19/24 ,  H01J49/00 ,  H01J49/0027 ,  H01J49/165 ,  Y02A90/22 ,  Y02A90/26 ,  Y10T436/117497

Abstract: The present invention relates to systems and methods for identifying new markers, diagnosing patients with a biological state of interest, and marketing/commercializing such diagnostics. The present invention relates to systems and methods of greater sensitivity, specificity, and/or cost effectiveness.

Abstract translation: 本发明涉及用于鉴定新标记，诊断具有生物学感兴趣的患者的系统和方法，以及销售/商业化这种诊断。 本发明涉及具有更大灵敏度，特异性和/或成本效益的系统和方法。

公开(公告)号：US20050244973A1

公开(公告)日：2005-11-03

申请号：US10845773

申请日：2004-05-14

Applicant: Frank Andel ,  Hans Bitter ,  Mikhail Belov ,  Michael Brown ,  Alfred Greenquist ,  Jonathan Heller ,  Alexander Sassi ,  John Stults ,  Kathy Stults

Inventor： Frank Andel ,  Hans Bitter ,  Mikhail Belov ,  Michael Brown ,  Alfred Greenquist ,  Jonathan Heller ,  Alexander Sassi ,  John Stults ,  Kathy Stults

IPC: G01N33/48 ,  G01N33/574

CPC classification number: G01N33/57434 ,  Y10T436/24

Abstract: The present invention provides methods for diagnosing cancers, such as prostate cancer. Also, methods for evaluating the prostate cancer state of a patient are described herein. These methods involve the detection, analysis, and classification of biological patterns in biological samples. The biological patterns are obtained using, for example, mass spectrometry systems, antibody based techniques, or nucleic acid based techniques. The present invention also includes therapeutic and prophylactic agents that target the biomarkers described herein. Also, the present invention provides methods for the treatment of prostate cancer using the markers described herein or agents that mimic the properties of these markers.

Abstract translation: 本发明提供了诊断癌症的方法，例如前列腺癌。 此外，本文描述了用于评估患者的前列腺癌状态的方法。 这些方法涉及生物样品中生物样本的检测，分析和分类。 使用例如质谱系统，基于抗体的技术或基于核酸的技术获得生物学模式。 本发明还包括靶向本文所述的生物标志物的治疗和预防剂。 此外，本发明提供了使用本文所述的标记物治疗前列腺癌的方法或模拟这些标记的性质的试剂。

公开(公告)号：US20110315552A1

公开(公告)日：2011-12-29

申请号：US13018622

申请日：2011-02-01

Applicant: John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

Inventor： John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

IPC: G01N27/447

CPC classification number: G01N33/50 ,  A61B5/150755 ,  G01N27/26 ,  G16B20/00 ,  G16B40/00 ,  H01J49/00 ,  H01J49/0027 ,  H01J49/165 ,  Y02A90/22 ,  Y02A90/26 ,  Y10T436/117497

Abstract: A business method for use in classifying patient samples. The method includes steps of collecting case samples representing a clinical phenotypic state and control samples representing patients without said clinical phenotypic state. Preferably the system uses a mass spectrometry platform system to identify patterns of polypeptides in said case samples and in the control samples without regard to the specific identity of at least some of said polypeptides. Based on identified representative patterns of the state, the business method provides for the marketing of diagnostic products using representative patterns. The present invention relates to systems and methods for identifying new markers, diagnosing patients with a biological state of interest, and marketing/commercializing such diagnostics. The present invention relates to systems and methods of greater sensitivity, specificity, and/or cost effectiveness.

Abstract translation: 用于分类患者样本的商业方法。 该方法包括收集代表临床表型状态的病例样本和对照样品的步骤，所述样品代表没有所述临床表型状态的患者。 优选地，系统使用质谱平台系统来鉴定所述病例样品和对照样品中多肽的模式，而不考虑至少一些所述多肽的特异性同一性。 基于确定的状态的代表性模式，业务方法使用代表性模式提供诊断产品的营销。 本发明涉及用于鉴定新标记，诊断具有生物学感兴趣的患者的系统和方法，以及销售/商业化这种诊断。 本发明涉及具有更大灵敏度，特异性和/或成本效益的系统和方法。

公开(公告)号：US06787016B2

公开(公告)日：2004-09-07

申请号：US09847780

申请日：2001-05-01

Applicant: Hongdong Roy Tan ,  Alexander Sassi ,  Ingrid Cruzado

Inventor： Hongdong Roy Tan ,  Alexander Sassi ,  Ingrid Cruzado

IPC: G01N27447

CPC classification number: G01N27/44747 ,  G01N27/44752

Abstract: Compositions and methods are provided for performing capillary electrophoresis using a composition comprising in combination in an aqueous buffered medium a coating polymer and a sieving polymer, where the sieving polymer is more hydrophilic than the coating polymer and is present in greater amount. Of particular interest are uncrosslinked acrylamide polymer mixtures for coating plastic channels and providing sieving for performing DNA separations in microfluidic devices. Polyacrylamide or N,N-dimethyl acrylamide is used with a N,N-dialkyl acrylamide copolymer, either separately or together for sieving and coating, serving as the medium in capillary electrophoresis DNA separations.

Abstract translation: 提供了组合物和方法，用于使用组合物在包含聚合物和筛分聚合物的水性缓冲介质中组合的组合物进行毛细管电泳，其中筛分聚合物比涂覆聚合物更亲水并且以更大的量存在。 特别令人感兴趣的是用于涂覆塑料通道的未交联的丙烯酰胺聚合物混合物，并提供用于在微流体装置中进行DNA分离的筛分。 聚丙烯酰胺或N，N-二甲基丙烯酰胺与N，N-二烷基丙烯酰胺共聚物分别或一起用于筛分和包衣，用作毛细管电泳DNA分离中的培养基。

公开(公告)号：US20170328885A1

公开(公告)日：2017-11-16

申请号：US15388954

申请日：2016-12-22

Applicant: John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

Inventor： John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

IPC: G01N33/50 ,  H01J49/00 ,  A61B5/15 ,  G01N27/26 ,  H01J49/16 ,  G06F19/24 ,  G06F19/18

CPC classification number: G01N33/50 ,  A61B5/150755 ,  G01N27/26 ,  G16B20/00 ,  G16B40/00 ,  H01J49/00 ,  H01J49/0027 ,  H01J49/165 ,  Y02A90/22 ,  Y02A90/26 ,  Y10T436/117497

Abstract: A business method for use in classifying patient samples. The method includes steps of collecting case samples representing a clinical phenotypic state and control samples representing patients without said clinical phenotypic state. Preferably the system uses a mass spectrometry platform system to identify patterns of polypeptides in said case samples and in the control samples without regard to the specific identity of at least some of said polypeptides. Based on identified representative patterns of the state, the business method provides for the marketing of diagnostic products using representative patterns. The present invention relates to systems and methods for identifying new markers, diagnosing patients with a biological state of interest, and marketing/commercializing such diagnostics. The present invention relates to systems and methods of greater sensitivity, specificity, and/or cost effectiveness.

公开(公告)号：US20090057550A1

公开(公告)日：2009-03-05

申请号：US12172988

申请日：2008-07-14

Applicant: John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

Inventor： John T. Stults ,  Alfred Greenquist ,  Alexander Sassi

IPC: B01D59/44

CPC classification number: G01N33/50 ,  A61B5/150755 ,  G01N27/26 ,  G06F19/18 ,  G06F19/24 ,  H01J49/00 ,  H01J49/0027 ,  H01J49/165 ,  Y02A90/22 ,  Y02A90/26 ,  Y10T436/117497

Abstract: A business method for use in classifying patient samples. The method includes steps of collecting case samples representing a clinical phenotypic state and control samples representing patients without said clinical phenotypic state. Preferably the system uses a mass spectrometry platform system to identify patterns of polypeptides in said case samples and in the control samples without regard to the specific identity of at least some of said polypeptides. Based on identified representative patterns of the state, the business method provides for the marketing of diagnostic products using representative patterns.The present invention relates to systems and methods for identifying new markers, diagnosing patients with a biological state of interest, and marketing/commercializing such diagnostics. The present invention relates to systems and methods of greater sensitivity, specificity, and/or cost effectiveness.

Abstract translation: 用于分类患者样本的商业方法。 该方法包括收集代表临床表型状态的病例样本和对照样品的步骤，所述样品代表没有所述临床表型状态的患者。 优选地，系统使用质谱平台系统来鉴定所述病例样品和对照样品中多肽的模式，而不考虑至少一些所述多肽的特异性同一性。 基于确定的状态的代表性模式，业务方法使用代表性模式提供诊断产品的营销。 本发明涉及用于鉴定新标记，诊断具有生物学感兴趣的患者的系统和方法，以及销售/商业化这种诊断。 本发明涉及具有更大灵敏度，特异性和/或成本效益的系统和方法。