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    Nucleic acid fragment encoding an NRPS for the biosynthesis of anthramycin
    3.
    发明授权
    Nucleic acid fragment encoding an NRPS for the biosynthesis of anthramycin 失效
    核苷酸片段编码NRPS用于生物合成奥匹他霉素

    公开(公告)号：US07108998B2

    公开(公告)日：2006-09-19

    申请号：US10166087

    申请日：2002-06-11

    申请人： Chirs M. Farnet ,  Alfredo Staffa

    发明人： Chirs M. Farnet ,  Alfredo Staffa

    IPC分类号： C07H21/04 ,  C12P1/00 ,  C12N1/20

    CPC分类号： C12N15/52 ,  C07K14/36

    摘要： Genes and proteins involved in the biosynthesis of benzodiazepines by microorganisms, including the genes and proteins forming the biosynthetic loci for the benzodiazepine anthramycin from Streptomyces refuineus subsp. thermotolerans including non-ribosomal peptide synthetases (NRPS). The genes and proteins allow direct manipulation of benzodiazepines and related chemical structures via chemical engineering of the enzymes involved in the biosynthesis of anthramycin.

    摘要翻译： 参与苯并二氮杂类生物合成的基因和蛋白质，包括从链霉菌（Streptomyces refuineus subsp。）中形成苯并二氮杂蒽环霉素生物合成基因座的基因和蛋白质。 耐热菌包括非核糖体肽合成酶（NRPS）。 基因和蛋白质可以通过化学工程来参与蒽环霉素的生物合成来直接操作苯并二氮杂和相关的化学结构。

    HIGH THROUGHPUT METHOD FOR DISCOVERY OF GENE CLUSTERS
    5.
    发明申请
    HIGH THROUGHPUT METHOD FOR DISCOVERY OF GENE CLUSTERS 审中-公开
    用于发现基因簇的高通量方法

    公开(公告)号：US20100016170A1

    公开(公告)日：2010-01-21

    申请号：US11803406

    申请日：2007-05-14

    申请人： Chris M. Farnet ,  Alfredo Staffa ,  Emmanuel Zazopoulos

    发明人： Chris M. Farnet ,  Alfredo Staffa ,  Emmanuel Zazopoulos

    IPC分类号： C40B20/04

    CPC分类号： C07K14/36 ,  C07K14/365 ,  C12N15/52 ,  C12P19/62

    摘要： A method for identifying gene cluster is disclosed. The method may be used for identifying gene clusters involved in the biosynthesis of natural products. A small insert library of DNA fragments of genomic DNA and a large insert library of DNA fragments of genomic DNA are prepared. Fragments in the small insert library are sequenced and compared by homology comparison under computer control to a database containing genes, gene fragments or proteins known to be involved in the biosynthesis of microbial natural products. Fragments having similar structure to genes, gene fragments or proteins known to be involved in the biosynthesis of naturally occurring metabolites are used as probes to screen the large insert library of genomic DNA to detect gene clusters involved in the biosynthesis of microbial natural products.

    摘要翻译： 公开了鉴定基因簇的方法。 该方法可用于鉴定涉及天然产物生物合成的基因簇。 制备基因组DNA的DNA片段的小插入文库和基因组DNA的DNA片段的大插入文库。 对小插入片段中的片段进行测序，并通过计算机控制下的同源性比较与包含已知参与微生物天然产物生物合成的基因，基因片段或蛋白质的数据库进行比较。 具有与基因相似结构的片段，已知参与天然存在的代谢物的生物合成的基因片段或蛋白质被用作筛选基因组DNA的大插入文库以探测参与微生物天然产物生物合成的基因簇的片段。

    System, knowledge repository and computer-readable medium for identifying a secondary metabolite from a microorganism
    6.
    发明申请
    System, knowledge repository and computer-readable medium for identifying a secondary metabolite from a microorganism 审中-公开
    用于从微生物鉴定次级代谢物的系统，知识库和计算机可读介质

    公开(公告)号：US20080010025A1

    公开(公告)日：2008-01-10

    申请号：US11551137

    申请日：2006-10-19

    申请人： Chris Farnet ,  James McAlpine ,  Brian Bachmann ,  Alfredo Staffa ,  Emmanuel Zazopoulos

    发明人： Chris Farnet ,  James McAlpine ,  Brian Bachmann ,  Alfredo Staffa ,  Emmanuel Zazopoulos

    IPC分类号： G01N33/50

    CPC分类号： C12Q1/025

    摘要： The invention relates to a method and system for identifying a secondary metabolite synthesized by a target gene cluster within a microorganism. A putative or confirmed function is attributed to a gene within the gene cluster, and an extract from the microorganism is obtained which is suspected to contain the secondary metabolite synthesized by the gene cluster. The extract is then assessed for chemical, physical or biological properties, and the metabolite is identified and optionally isolated. Further, the invention provides a knowledge repository in which gene cluster information is linked to secondary metabolite production data. The invention further relates to a graphical user interface for accessing the knowledge repository, and a memory for storing data, having a data structure that is stored in the memory.

    摘要翻译： 本发明涉及鉴定由微生物内的靶基因簇合成的次级代谢物的方法和系统。 推定或确认的功能归因于基因簇内的基因，并且获得了怀疑含有由基因簇合成的次级代谢物的微生物提取物。 然后评估提取物的化学，物理或生物学性质，并鉴定和任选分离代谢物。 此外，本发明提供了知识库，其中基因簇信息与次级代谢产生数据相关联。 本发明还涉及用于访问知识库的图形用户界面和用于存储数据的存储器，其具有存储在存储器中的数据结构。

    Nucleic acids encoding an enediyne polyketide synthase complex
    8.
    发明授权
    Nucleic acids encoding an enediyne polyketide synthase complex 失效
    编码烯二酮聚酮化合物复合物的核酸

    公开(公告)号：US07462705B2

    公开(公告)日：2008-12-09

    申请号：US11053576

    申请日：2005-02-08

    申请人： Chris M. Farnet ,  Alfredo Staffa ,  Emmanuel Zazopoulos

    发明人： Chris M. Farnet ,  Alfredo Staffa ,  Emmanuel Zazopoulos

    IPC分类号： C07H21/04

    CPC分类号： C07K14/36 ,  C12N15/52

    摘要： Five protein families cooperate to form the warhead structure that characterizes enediyne compounds, both chromoprotein enediynes and non-chromoprotein enediynes. The protein families include a polyketide synthase and thioesterase protein which form a polyketide synthase catalytic complex involved in warhead formation in enediynes. Genes encoding a member of each of the five protein families are found in all enediyne biosynthetic loci. The genes and proteins may be used in genetic engineering applications to design new enediyne compounds and in methods to identify new enediyne biosynthetic loci.

    摘要翻译： 五个蛋白质家族合作形成弹头结构，其特征是烯雌酮化合物，包括染色体蛋白烯和非色素蛋白烯二烯。 蛋白质家族包括聚酮化合物合酶和硫酯酶蛋白，其形成涉及在炔诺酮中形成弹头的聚酮化合物合酶催化复合物。 在所有烯二烯生物合成基因座中都发现编码五个蛋白家族中每一个的成员的基因。 基因和蛋白质可用于遗传工程应用中以设计新的烯二烯化合物和鉴定新的烯二炔生物合成基因座的方法。

    High throughput method for discovery of gene clusters
    9.
    发明授权
    High throughput method for discovery of gene clusters 失效
    用于发现基因簇的高通量方法

    公开(公告)号：US07257562B2

    公开(公告)日：2007-08-14

    申请号：US10232370

    申请日：2002-09-03

    申请人： Chris M. Farnet ,  Alfredo Staffa ,  Emmanuel Zazopoulos

    发明人： Chris M. Farnet ,  Alfredo Staffa ,  Emmanuel Zazopoulos

    IPC分类号： G06N3/00 ,  G01N33/48 ,  C12Q1/00

    CPC分类号： C07K14/36 ,  C07K14/365 ,  C12N15/52 ,  C12P19/62

    摘要： A method for identifying gene cluster is disclosed. The method may be used for identifying gene clusters involved in the biosynthesis of natural products. A small insert library of DNA fragments of genomic DNA and a large insert library of DNA fragments of genomic DNA are prepared. Fragments in the small insert library are sequenced and compared by homology comparison under computer control to a database containing genes, gene fragments or proteins known to be involved in the biosynthesis of microbial natural products. Fragments having similar structure to genes, gene fragments or proteins known to be involved in the biosynthesis of naturally occurring metabolites are used as probes to screen the large insert library of genomic DNA to detect gene clusters involved in the biosynthesis of microbial natural products.

    摘要翻译： 公开了鉴定基因簇的方法。 该方法可用于鉴定涉及天然产物生物合成的基因簇。 制备基因组DNA的DNA片段的小插入文库和基因组DNA的DNA片段的大插入文库。 对小插入片段中的片段进行测序，并通过计算机控制下的同源性比较与包含已知参与微生物天然产物生物合成的基因，基因片段或蛋白质的数据库进行比较。 具有与基因相似结构的片段，已知参与天然存在的代谢物的生物合成的基因片段或蛋白质被用作筛选基因组DNA的大插入文库以探测参与微生物天然产物生物合成的基因簇的片段。

    Genes and proteins involved in the biosynthesis of lipopeptides
    10.
    发明授权
    Genes and proteins involved in the biosynthesis of lipopeptides 失效
    参与脂肽生物合成的基因和蛋白质

    公开(公告)号：US07235651B2

    公开(公告)日：2007-06-26

    申请号：US10329079

    申请日：2002-12-24

    申请人： Chris M. Farnet ,  Alfredo Staffa ,  Emmanuel Zazopoulos

    发明人： Chris M. Farnet ,  Alfredo Staffa ,  Emmanuel Zazopoulos

    IPC分类号： C07H21/02

    CPC分类号： C07K14/36 ,  C12N15/52

    摘要： Genes and proteins involved in the biosynthesis of lipopeptides by microorganisms, in particular the nucleic acids forming the biosynthetic locus for the A54145 lipopeptide from Streptomyces fradiae and the A54145-like lipopeptide from Streptomyces refuineus. These nucleic acids can be used to make expression constructs and transformed host cells for the production of lipopeptides. The genes and proteins allow direct manipulation of lipopeptides and related chemical structures via chemical engineering of the proteins involved in the biosynthesis of A54145.

    摘要翻译： 涉及通过微生物生物合成脂肽的基因和蛋白质，特别是形成来自链霉菌的A54145脂肽的生物合成基因座的核酸和来自链霉菌（Streptomyces refuineus）的A54145样脂肽。 这些核酸可用于制备用于产生脂肽的表达构建体和转化的宿主细胞。 基因和蛋白质可以通过参与A54145生物合成的蛋白质的化学工程直接操纵脂肽和相关的化学结构。