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    Production of vaccines
    3.
    发明授权
    Production of vaccines 有权

    公开(公告)号：US07550284B2

    公开(公告)日：2009-06-23

    申请号：US11450038

    申请日：2006-06-08

    申请人： Maria Grazia Pau ,  Alphonsus G. C. M. UytdeHaag

    发明人： Maria Grazia Pau ,  Alphonsus G. C. M. UytdeHaag

    IPC分类号： C12N7/00

    CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/145 ,  C12N2710/10343 ,  C12N2760/16134 ,  C12N2760/16152

    摘要： Means and methods are provided for the production of mammalian viruses comprising: infecting a culture of immortalized human cells with the virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The viruses can be harvested and be used for the production of vaccines. Advantages are that human cells of the present invention can be cultured under defined serum free conditions, and the cells show improved capability for propagating virus. In particular, methods are provided for producing, in cultured human cells, influenza virus and vaccines derived thereof. This method eliminates the necessity to use whole chicken embryos for the production of influenza vaccines. The method provides also for the continuous or batchwise removal of culture media. As such, the invention allows the large-scale, continuous production of viruses to a high titer.

    Production of vaccines
    4.
    发明授权
    Production of vaccines 有权
    生产疫苗

    公开(公告)号：US07527961B2

    公开(公告)日：2009-05-05

    申请号：US11271368

    申请日：2005-11-11

    申请人： Maria Grazia Pau ,  Alphonsus G. C. M. UytdeHaag ,  Govert Johan Schouten

    发明人： Maria Grazia Pau ,  Alphonsus G. C. M. UytdeHaag ,  Govert Johan Schouten

    IPC分类号： C12N7/00

    CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5252 ,  A61K2039/70 ,  C12N2720/12351 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2770/20051 ,  C12N2770/24051 ,  C12N2770/24151

    摘要： Means and methods for producing mammalian viruses, the method comprising infecting a culture of immortalized human cells with a virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The viruses can be harvested and be used for the production of vaccines. Advantages include that human cells of the present invention can be cultured under defined serum-free conditions and the cells show improved capability for propagating virus. Methods are provided for producing, in cultured human cells, influenza virus and vaccines derived thereof. This method eliminates the necessity of using whole chicken embryos for the production of Influenza vaccines. The method also provides for the continuous or batch-wise removal of culture media. As such, the present invention allows the large-scale continuous production of viruses to a high titer.

    摘要翻译： 用于生产哺乳动物病毒的方法和方法，所述方法包括用病毒感染永生化人细胞的培养物，在病毒生长的条件下孵育感染病毒的培养物繁殖病毒，并形成含病毒的培养基， 并除去含病毒的培养基。 可以收获病毒并用于生产疫苗。 优点包括本发明的人细胞可以在规定的无血清条件下进行培养，并且细胞显示出增强的病毒传播能力。 提供了用于在培养的人类细胞中产生流感病毒及其衍生的疫苗的方法。 该方法消除了使用全鸡胚胎生产流感疫苗的必要性。 该方法还提供了连续或间歇地除去培养基。 因此，本发明允许病毒的大规模连续生产达到高滴度。

    Production of vaccines
    9.
    发明授权
    Production of vaccines 有权
    生产疫苗

    公开(公告)号：US07192759B1

    公开(公告)日：2007-03-20

    申请号：US09449854

    申请日：1999-11-26

    申请人： Maria Grazia Pau ,  Alphonsus G. C. M. UytdeHaag

    发明人： Maria Grazia Pau ,  Alphonsus G. C. M. UytdeHaag

    IPC分类号： C12N7/00

    CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/145 ,  C12N2710/10343 ,  C12N2760/16134 ,  C12N2760/16152

    摘要： Novel means and methods are provided for the production of mammalian viruses comprising, infecting a culture of immortalized human cells with the virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The viruses can be harvested and be used for the production of vaccines. Advantages are that human cells of the present invention can be cultured under defined serum free conditions, and the cells show improved capability for propagating virus. In particular, methods are provided for producing, in cultured human cells, influenza virus and vaccines derived thereof. This method eliminates the necessity to use whole chicken embryos for the production of influenza vaccines. The method provides also for the continuous or batchwise removal of culture media. As such, the present invention allows the large-scale, continuous production of viruses to a high titer.

    摘要翻译： 提供了用于生产哺乳动物病毒的新方法和方法，其包括用病毒感染永生化人细胞的培养物，在病毒生长的条件下培养感染病毒的培养物以繁殖病毒，并形成病毒 - 并除去含病毒的培养基。 可以收获病毒并用于生产疫苗。 本发明的人细胞的优点在于能够在规定的无血清条件下进行培养，细胞表现出增强的病毒能力。 特别地，提供了用于在培养的人类细胞中产生流感病毒及其衍生的疫苗的方法。 该方法消除了使用全鸡胚胎生产流感疫苗的必要性。 该方法还用于连续或间歇地除去培养基。 因此，本发明允许大规模，连续的病毒产生高滴度。