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    Devices, methods and systems for low volume microarray processing
    1.
    发明申请
    Devices, methods and systems for low volume microarray processing 审中-公开
    用于低体积微阵列处理的设备，方法和系统

    公开(公告)号：US20050163664A1

    公开(公告)日：2005-07-28

    申请号：US11030251

    申请日：2005-01-06

    申请人： Kurt Halverson ,  Anila Prabhu ,  Steven Botzet

    发明人： Kurt Halverson ,  Anila Prabhu ,  Steven Botzet

    IPC分类号： B01L3/00

    CPC分类号： B01L3/5027 ,  B01J2219/00527 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00641 ,  B01J2219/00677 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00889 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0457 ,  B82Y30/00 ,  Y10T436/11 ,  Y10T436/110833 ,  Y10T436/112499 ,  Y10T436/143333 ,  Y10T436/2575

    摘要： Devices, methods and systems for low volume microarray processing are disclosed. The microarray devices preferably include a plurality of reactant sites on a reactant surface. The reactant sites include reactants that operate to capture one or more selected analytes that can then be detected based on an electromagnetic signal, e.g., fluorescence, that is emitted by each analyte in response to excitation energy incident on the microarray device. Mixing and/or distribution of the analyte sample over the reactant surface is accomplished by tilting the reactant surface such that the analyte sample flows over the reactant surface under the force of gravity. The tilting is performed such that a portion of the analyte sample accumulates in a bead along a first edge of the reactant surface. The reactant surface is then tilted in a different direction such that a portion of the analyte sample flows over the reactant surface and accumulates at a second edge. The reactant surface preferably generates sufficient capillary forces with the analyte sample such that the analyte sample is retained on the reactant surface. The tilting and resultant flow may be performed as many times as necessary to obtain the desired mixing and/or distribution of analyte sample over the reactant surface.

    摘要翻译： 公开了用于低体积微阵列处理的装置，方法和系统。 微阵列装置优选在反应物表面上包括多个反应物位点。 反应物位点包括反应物，其操作以捕获一种或多种选择的分析物，其然后可以基于由每个分析物响应入射在微阵列装置上的激发能量发射的电磁信号（例如荧光）而被检测。 分析物样品在反应物表面上的混合和/或分布通过倾斜反应物表面来实现，使得分析物样品在重力的作用下在反应物表面上流动。 进行倾斜使得一部分分析物样品沿着反应物表面的第一边缘积聚在珠粒中。 然后将反应物表面沿不同的方向倾斜，使得一部分分析物样品在反应物表面上流动并在第二边缘积聚。 反应物表面优选地与分析物样品产生足够的毛细管力，使得分析物样品保留在反应物表面上。 可以根据需要多次进行倾斜和产生的流动，以获得分析物样品在反应物表面上的期望的混合和/或分布。