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8 条记录 (耗时 0.026 秒)

    Method for extracting DNA from dried specimens
    3.
    发明授权
    Method for extracting DNA from dried specimens 失效
    从干燥样品中提取DNA的方法

    公开(公告)号：US06410725B1

    公开(公告)日：2002-06-25

    申请号：US09513179

    申请日：2000-02-25

    申请人： Thomas Scholl ,  Michael T. Pyne ,  Arnold R Oliphant

    发明人： Thomas Scholl ,  Michael T. Pyne ,  Arnold R Oliphant

    IPC分类号： C07H2104

    CPC分类号： C12N15/1003 ,  C12Q1/6806

    摘要： The present invention relates to a composition and to a method for extracting DNA. More specifically, the present invention relates to a composition and to a method to extract DNA from dried biological samples on solid substrates, including but not limited to, buccal smears, semen and especially blood. The method can be conducted in a single-tube. The DNA extracted in accordance with the present invention can be used for DNA amplification reactions, DNA sequencing, DNA restriction analysis and DNA hyridization.

    摘要翻译： 本发明涉及组合物和提取DNA的方法。 更具体地，本发明涉及从固体底物上干燥的生物样品中提取DNA的组合物和方法，包括但不限于颊涂片，精液，特别是血液。 该方法可以在单管中进行。 根据本发明提取的DNA可用于DNA扩增反应，DNA测序，DNA限制性分析和DNA Hyridization。

    Mycoplasma arthritidis T-cell mitogen
    4.
    发明授权
    Mycoplasma arthritidis T-cell mitogen 失效
    支原体支原体T细胞丝裂原

    公开(公告)号：US5639869A

    公开(公告)日：1997-06-17

    申请号：US165038

    申请日：1993-12-10

    申请人： Barry C. Cole ,  Curtis L. Atkin ,  Arnold R. Oliphant ,  Ann Pole

    发明人： Barry C. Cole ,  Curtis L. Atkin ,  Arnold R. Oliphant ,  Ann Pole

    IPC分类号： A61K38/00 ,  C07H21/04 ,  C07K14/30

    CPC分类号： C07H21/04 ,  C07K14/30 ,  A61K38/00 ,  Y10S530/825

    摘要： A method of purifying Mycoplasma arthritidis mitogen (MAM) to electrophoretic and sequence homogeneity is disclosed. A preparation of MAM purified according to this method was used to determine the sequence of the N-terminal 54 amino acids of MAM. A synthetic peptide consisting of amino acids 15-32 inhibited MAM-induced cell proliferation in vitro. The sequence of the N-terminal 54 amino acids was reverse translated, nucleotide probes were designed therefrom, and the MAM gene was selected from a genomic library. The MAM gene was sequenced and found to be contained on a 1107 bp DNA fragment. The primary translation product contains a 39 amino acid signal sequence and a 213 amino acid mature MAM (molecular weight 25,294). Amino acid sequence comparisons of MAM to bacterial and murine tumor virus superantigens showed regions of conservative sequence homology, including the region capable of inhibiting cell proliferation. Sequence homologies to HIV and other retrovirus proteins and to certain regulatory proteins were also detected. Strategies for blocking or immunizing against certain diseases, including autoimmune diseases, are disclosed.

    摘要翻译： 公开了一种纯化支原体支原体丝裂原（MAM）以电泳和序列同质性的方法。 使用根据该方法纯化的MAM制剂来确定MAM的N-末端54个氨基酸的序列。 由氨基酸15-32组成的合成肽在体外抑制MAM诱导的细胞增殖。 将N-末端54个氨基酸的序列反向翻译，设计核苷酸探针，并从基因组文库中选择MAM基因。 对MAM基因进行测序，发现其含有1107bp的DNA片段。 初级翻译产物含有39个氨基酸的信号序列和213个氨基酸的成熟MAM（分子量为25,294）。 MAM与细菌和鼠肿瘤病毒超级抗原的氨基酸序列比较显示出保守序列同源性的区域，包括能够抑制细胞增殖的区域。 还检测到与HIV和其他逆转录病毒蛋白质和某些调节蛋白质的序列同源性。 公开了阻止或免疫某些疾病（包括自身免疫性疾病）的策略。

    Mycoplasma arthritidis T-cell mitogen
    8.
    发明授权
    Mycoplasma arthritidis T-cell mitogen 失效
    支原体支原体T细胞丝裂原

    公开(公告)号：US5872233A

    公开(公告)日：1999-02-16

    申请号：US876781

    申请日：1997-06-17

    申请人： Barry C. Cole ,  Curtis L. Atkin ,  Arnold R. Oliphant ,  Ann Pole

    发明人： Barry C. Cole ,  Curtis L. Atkin ,  Arnold R. Oliphant ,  Ann Pole

    IPC分类号： A61K38/00 ,  C07H21/04 ,  C07K14/30

    CPC分类号： C07H21/04 ,  C07K14/30 ,  A61K38/00 ,  Y10S530/825

    摘要： A method of purifying Mycoplasma arthritidis mitogen (MAM) to electrophoretic and sequence homogeneity is disclosed. A preparation of MAM purified according to this method was used to determine the sequence of the N-terminal 54 amino acids of MAM. A synthetic peptide consisting of amino acids 15-32 inhibited MAM-induced cell proliferation in vitro. The sequence of the N-terminal 54 amino acids was reverse translated, nucleotide probes were designed therefrom, and the MAM gene was selected from a genomic library. The MAM gene was sequenced and found to be contained on a 1107 bp DNA fragment. The primary translation product contains a 39 amino acid signal sequence and a 213 amino acid mature MAM (molecular weight 25,294). Amino acid sequence comparisons of MAM to bacterial and murine tumor virus superantigens showed regions of conservative sequence homology, including the region capable of inhibiting cell proliferation. Sequence homologies to HIV and other retrovirus proteins and to certain regulatory proteins were also detected. Strategies for blocking or immunizing against certain diseases, including autoimmune diseases, are disclosed.

    摘要翻译： 公开了一种纯化支原体支原体丝裂原（MAM）以电泳和序列同质性的方法。 使用根据该方法纯化的MAM制剂来确定MAM的N-末端54个氨基酸的序列。 由氨基酸15-32组成的合成肽在体外抑制MAM诱导的细胞增殖。 将N-末端54个氨基酸的序列反向翻译，设计核苷酸探针，并从基因组文库中选择MAM基因。 对MAM基因进行测序，发现其含有1107bp的DNA片段。 初级翻译产物含有39个氨基酸的信号序列和213个氨基酸的成熟MAM（分子量为25,294）。 MAM与细菌和鼠肿瘤病毒超级抗原的氨基酸序列比较显示出保守序列同源性的区域，包括能够抑制细胞增殖的区域。 还检测到与HIV和其他逆转录病毒蛋白质和某些调节蛋白质的序列同源性。 公开了阻止或免疫某些疾病（包括自身免疫性疾病）的策略。