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公开(公告)号：US20110294209A1
公开(公告)日：2011-12-01
申请号：US13206056
申请日：2011-08-09
申请人： Bertrand Pain , Fabienne Guehenneux
发明人： Bertrand Pain , Fabienne Guehenneux
IPC分类号： C12N5/0735
CPC分类号： C12N5/0606 , C12N2500/90 , C12N2500/92 , C12N2500/95 , C12N2510/02
摘要： The present invention relates to a method for producing avian cell lines, comprising gradual or complete withdrawal of growth factors, serum and/or feeder layer so that the established lines are adherent or nonadherent cells capable of proliferating indefinitely in a basic culture medium. The invention also relates to the cells derived from such lines which are particularly useful for the production of substances of interest.
摘要翻译： 本发明涉及一种生产禽类细胞系的方法，包括逐渐或完全撤出生长因子，血清和/或饲养层，使得建立的系是能够在碱性培养基中无限增殖的粘附或非粘附细胞。 本发明还涉及特别可用于生产感兴趣物质的来自这些品系的细胞。
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2.发明申请METHOD FOR REPROGRAMMING IN VITRO STEM CELLS AND SOMATIC CELLS INTO GERMINAL CELLS 审中-公开将体细胞和体细胞转染入终末细胞的方法公开(公告)号：US20110010783A1
公开(公告)日：2011-01-13
申请号：US12808656
申请日：2008-12-11
IPC分类号： A01K67/00 , C12N5/071 , C12N5/0735 , C12N15/00
CPC分类号： C12N5/0611 , A01K67/0271 , A01K2217/052 , A01K2227/30 , C12N2501/60 , C12N2506/02 , C12N2510/00
摘要： The present invention is related to a method for inducing in vitro the differentiation of stem cells or somatic cells towards germinal cells, comprising the following steps: cultivating said stem cells or somatic cells in a medium allowing their differentiation, and collecting the germinal cells from the culture medium, wherein said cells are cells transformed with an exogenous genetic construction comprising at least a Vasa gene. Germinal cells such as obtained and chimeric animals are also an object of this invention.
摘要翻译： 本发明涉及体外诱导干细胞或体细胞向生发细胞分化的方法，其包括以下步骤：在允许其分化的培养基中培养所述干细胞或体细胞，并从生物细胞中收集生发细胞 培养基，其中所述细胞是用至少包含Vasa基因的外源基因构建体转化的细胞。 诸如获得的和嵌合动物的生殖细胞也是本发明的目的。
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3.发明授权
公开(公告)号：US6114168A
公开(公告)日：2000-09-05
申请号：US817671
申请日：1997-05-12
申请人： Jacques Samarut , Bertrand Pain
发明人： Jacques Samarut , Bertrand Pain
IPC分类号： A01K67/027 , C12N5/02 , C12N5/073 , C12N5/0735 , C12N5/00 , C12N5/06 , C12N5/16
CPC分类号： A01K67/0275 , C12N5/0603 , C12N5/0606 , A01K2217/05 , C12N2500/40 , C12N2500/44 , C12N2501/105 , C12N2501/115 , C12N2501/125 , C12N2501/13 , C12N2501/2306 , C12N2501/2311 , C12N2501/235 , C12N2501/237 , C12N2501/385 , C12N2502/1323 , C12N2510/00
摘要： A culture medium for culturing chicken embryonic stem (ES) cells is disclosed. The culture medium is used for supporting avian ES cell cultures. The components of the avian ES cell media include cytokines, fibroblast growth factors, insulin-like growth factors and stem cell growth factors and anti-retinoic acid antibody. The culture medium is substantially free of active retinoic acid. A method for culturing avian ES cells and the resulting products are also disclosed.
摘要翻译： PCT No.PCT / FR95 / 01389第 371日期：1997年5月12日 102（e）日期1997年5月12日PCT 1995年10月20日PCT PCT。 公开号WO96 / 12793 日期1996年5月2日公开了培养鸡胚胎干细胞（ES）细胞的培养基。 培养基用于支持禽类ES细胞培养。 禽类ES细胞培养基的成分包括细胞因子，成纤维细胞生长因子，胰岛素样生长因子和干细胞生长因子以及抗视黄酸抗体。 培养基基本上不含活性视黄酸。 还公开了培养禽ES细胞的方法和所得产物。
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公开(公告)号：US09382513B2
公开(公告)日：2016-07-05
申请号：US12717096
申请日：2010-03-03
申请人： Bertrand Pain , Fabienne Guehenneux
发明人： Bertrand Pain , Fabienne Guehenneux
IPC分类号： C12N5/00 , C12N5/0735
CPC分类号： C12N5/0606 , C12N2500/90 , C12N2500/92 , C12N2500/95 , C12N2510/02
摘要： The present invention relates to a method for producing avian cell lines, comprising gradual or complete withdrawal of growth factors, serum and/or feeder layer so that the established lines are adherent or nonadherent cells capable of proliferating indefinitely in a basic culture medium. The invention also relates to the cells derived from such lines which are particularly useful for the production of substances of interest.
摘要翻译： 本发明涉及一种生产禽类细胞系的方法，包括逐渐或完全撤出生长因子，血清和/或饲养层，使得建立的系是能够在碱性培养基中无限增殖的粘附或非粘附细胞。 本发明还涉及特别可用于生产感兴趣物质的来自这些品系的细胞。
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公开(公告)号：US20090311682A1
公开(公告)日：2009-12-17
申请号：US12294033
申请日：2007-03-19
CPC分类号： C12Q1/6881 , C12Q2600/158
摘要： The present invention relates to a novel combination of avian cell markers which make it possible to characterize the said cells according to their phenotype, whether they are StX cells, stem cells or germ cells. The present invention also relates to a method for characterizing avian cells with the said markers, and to a method for culturing avian cells, in which the cells are characterized by the method according to the invention.
摘要翻译： 本发明涉及鸟类细胞标志物的新型组合，其使得可以根据其表型来表征所述细胞，无论它们是StX细胞，干细胞还是生殖细胞。 本发明还涉及用所述标记表征禽细胞的方法，以及培养禽细胞的方法，其中细胞的特征在于根据本发明的方法。
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6.发明授权Active retinoic acid-free culture medium for avian totipotent embryonic stem cells 失效用于禽类全能胚胎干细胞的无视黄酸无活性培养基公开(公告)号：US06998266B2
公开(公告)日：2006-02-14
申请号：US10097245
申请日：2002-03-15
申请人： Jacques Samarut , Bertrand Pain
发明人： Jacques Samarut , Bertrand Pain
IPC分类号： C12N5/02
CPC分类号： A01K67/0275 , A01K2217/05 , C12N5/0603 , C12N5/0606 , C12N2500/40 , C12N2500/44 , C12N2501/105 , C12N2501/115 , C12N2501/125 , C12N2501/13 , C12N2501/2306 , C12N2501/2311 , C12N2501/235 , C12N2501/237 , C12N2501/385 , C12N2502/1323 , C12N2510/00
摘要： A culture medium for avian embryonic cells and an avian cell culture medium is disclosed. The culture medium is characterized in that it has elements complementary to avian embryonic cells. The complementary elements include insulin-like growth factors and stem cell growth factors. The medium is substantially free of active retinoic acid. A method of culturing avian embryonic cells and the resulting products are disclosed.
摘要翻译： 公开了禽类胚胎细胞和鸟类细胞培养基的培养基。 培养基的特征在于其具有与禽类胚胎细胞互补的元件。 互补元件包括胰岛素样生长因子和干细胞生长因子。 该培养基基本上不含活性视黄酸。 公开了培养禽胚胎细胞的方法和所得产物。
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公开(公告)号：US20100221825A1
公开(公告)日：2010-09-02
申请号：US12717096
申请日：2010-03-03
申请人： Bertrand PAIN , Fabienne Guehenneux
发明人： Bertrand PAIN , Fabienne Guehenneux
IPC分类号： C12N5/071
CPC分类号： C12N5/0606 , C12N2500/90 , C12N2500/92 , C12N2500/95 , C12N2510/02
摘要： The present invention relates to a method for producing avian cell lines, comprising gradual or complete withdrawal of growth factors, serum and/or feeder layer so that the established lines are adherent or nonadherent cells capable of proliferating indefinitely in a basic culture medium. The invention also relates to the cells derived from such lines which are particularly useful for the production of substances of interest.
摘要翻译： 本发明涉及一种生产禽类细胞系的方法，包括逐渐或完全撤出生长因子，血清和/或饲养层，使得建立的系是能够在碱性培养基中无限增殖的粘附或非粘附细胞。 本发明还涉及特别可用于生产感兴趣物质的来自这些品系的细胞。
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公开(公告)号：US20090239297A1
公开(公告)日：2009-09-24
申请号：US12350781
申请日：2009-01-08
申请人： Bertrand PAIN , Fabienne Guehenneux
发明人： Bertrand PAIN , Fabienne Guehenneux
CPC分类号： C12N5/0606 , C12N2500/90 , C12N2500/92 , C12N2500/95 , C12N2510/02
摘要： The present invention relates to a method for producing avian cell lines, comprising gradual or complete withdrawal of growth factors, serum and/or feeder layer so that the established lines are adherent or nonadherent cells capable of proliferating indefinitely in a basic culture medium. The invention also relates to the cells derived from such lines which are particularly useful for the production of substances of interest.
摘要翻译： 本发明涉及一种生产禽类细胞系的方法，包括逐渐或完全撤出生长因子，血清和/或饲养层，使得建立的系是能够在碱性培养基中无限增殖的粘附或非粘附细胞。 本发明还涉及特别可用于生产感兴趣物质的来自这些品系的细胞。
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9.发明授权公开(公告)号：US07432101B2
公开(公告)日：2008-10-07
申请号：US10565281
申请日：2004-07-22
申请人： Fabienne Guehenneux , Bertrand Pain
发明人： Fabienne Guehenneux , Bertrand Pain
IPC分类号： C12N7/00 , A61K39/285 , C12N5/00
CPC分类号： C12N15/86 , A61K2039/525 , A61K2039/5256 , C12N7/00 , C12N2710/24143 , C12N2710/24152 , Y02A50/388 , Y02A50/39 , Y02A50/412
摘要： The present invention relates to a method for replicating pox viruses such as vaccinia virus comprising the steps of inoculating avian embryonic stem cells with viral particles and culturing said cells in a basal medium until cells lysis occurs and newly produced viral particles are released in said medium.
摘要翻译： 本发明涉及一种复制痘病毒痘苗病毒的方法，包括以下步骤：用病毒颗粒接种禽胚胎干细胞，并在基础培养基中培养所述细胞，直到发生细胞裂解，并且新产生的病毒颗粒在所述培养基中释放。
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10.发明申请公开(公告)号：US20060233834A1
公开(公告)日：2006-10-19
申请号：US10565281
申请日：2004-07-22
申请人： Fabienne Guehenneux , Bertrand Pain
发明人： Fabienne Guehenneux , Bertrand Pain
IPC分类号： A61K39/275 , C12N15/86 , C12N7/00
CPC分类号： C12N15/86 , A61K2039/525 , A61K2039/5256 , C12N7/00 , C12N2710/24143 , C12N2710/24152 , Y02A50/388 , Y02A50/39 , Y02A50/412
摘要： The present invention relates to a method for replicating pox viruses such as vaccinia virus comprising the steps of inoculating avian embryonic stem cells with viral particles and culturing said cells in a basal medium until cells lysis occurs and newly produced viral particles are released in said medium.
摘要翻译： 本发明涉及一种复制痘病毒痘苗病毒的方法，包括以下步骤：用病毒颗粒接种禽胚胎干细胞，并在基础培养基中培养所述细胞，直到发生细胞裂解，并且新产生的病毒颗粒在所述培养基中释放。
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